FastQCFastQC Report
Tue 31 May 2016
SRR212534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150306
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT24181.6087182148417227No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC20141.339933202932684No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA18141.2068713158490014No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA13060.8688941226564476No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC12920.8595797905605898No Hit
GAGGAGGGGGACACATTTTTAAAAAATATAAATTGTATGTTCATTATAAAGAAATT9270.6167418466328689No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8970.5967825635703166No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC7150.47569624632416535No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.3552752385134326No Hit
GAGGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4360.2900749138424281No Hit
GAGGAGGGGACACCTATGTCAACACACCAACTGGGGATGAGGATGGCAGAAGTCGC4030.2681197024736205No Hit
GAGGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3630.24150732505688396No Hit
GAGGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3420.22753582691309726No Hit
GAGGAGGGGAACTATCTGTAAACCAACCTTTCAGTTGCTATATAACTTCCATAGAC3370.22420927973600524No Hit
GAGGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3150.20957247215680014No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3050.20291937780261599No Hit
GAGGAGGGGGGTCACAACTGAAATCAGCATCCTGATTACTTTGTGTTCCAGTGTTC2860.19027849852966616No Hit
GAGGAGGGGGCTCATTATATTCAAATATTCATTTTAGGAGCCATTCCGTAGTGCCA2850.18961318909424774No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC2830.1882825702234109No Hit
GAGGAGGGGACGATACCTTTTTCTTGGTGATTATGTGGACAGAGGTTATTTTAGTA2740.18229478530464518No Hit
GAGGAGGGGATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGA2720.1809641664338084No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.17031921546711376No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2380.1583436456295823No Hit
GAGGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2300.153021170146235No Hit
GAGGAGGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCGGCCAAAGTGAACAACT2250.14969462296914296No Hit
GAGGAGGGGGACTTCCTGTCAGCGAGCTGAATCCCCTGGAGATGGGGGGGTGGGCT2240.14902931353372453No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGATCTTCCA2240.14902931353372453No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG2200.14636807579205088No Hit
GAGGAGGGGAACTTCTTAAAAGACAAAAAAGAGAGAAATTGTATCCCCGTGCATGC2170.14437214748579563No Hit
GAGGAGGGGGTCTCCTGCTTTAACTATTTCTAAAGTTCTTTTATCCATTTGTTCAC2110.14038029087328516No Hit
GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2040.13572312482535626No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.13306188708368263No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCC1990.1323965776482642No Hit
GAGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1940.12907003047117213No Hit
GAGGAGGGGAACCTAGGCTGGCCCTGAACTCAGGGCAGTCCTTCTGTCTCAGCTTC1870.12441286442324326No Hit
GAGGAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1800.11975569837531436No Hit
GAGGAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA1750.1164291511982223No Hit
GAGGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1750.1164291511982223No Hit
GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1750.1164291511982223No Hit
GAGGAGGGGAGGTTGACTTTGAAATCGTAGAGCTCTACCTACTTTTACTTCCACAA1660.11044136627945658No Hit
GAGGAGGGGCAATTGGGTTGTGCCTTTGCGTTCGAATGCTGACCCATGCAATATGG1650.10977605684403817No Hit
GAGGAGGGGCCACCTTCCATGAGCAGCTTGCCCAGGCACTCCAGGTCTGAGAAGCA1640.10911074740861974No Hit
GAGGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1610.1071148191023645No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1600.10644950966694611No Hit
GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1560.10378827192527244No Hit
GAGGAGGGGAAGGTGGGCAGCCGTTCAGTGAAGTAGGAGAGGTGAAAGACTTTCCA1530.1017923436190172No Hit
GAGGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1520.1011270341835988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGTA150.00821483150.3080350
TTGAGTG150.00821483150.3080350
ACAGGAC150.00821483150.3080350
TATAAAC150.00821483150.3080350
AGAAGTC650.050.29113848
TACTTGT150.00822574950.29113449
CGTGCAT355.0133167E-850.29113448
TTATGCT204.0542672E-450.29113448
ACGTGGT150.00822574950.29113448
CAATATG150.00822574950.29113449
GAAATAG150.00825856650.24052443
CCTTACT150.00825856650.24052439
AAATACT150.00825856650.24052446
CATGCAA150.00825856650.24052445
CCGTGCA355.0531526E-850.24052447
TTACTTC355.0531526E-850.24052445
TTCAATT150.00825856650.24052442
TCCTCAT150.00825856650.24052441
ATGCTAA301.0077856E-650.24052440
GAATAGC150.00825856650.24052445
Produced by FastQC (version 0.11.5)