FastQCFastQC Report
Tue 31 May 2016
SRR212533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139022
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT21171.5227805671044872No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17961.2918818604249687No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA17941.290443239199551No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC16851.2120383824142942No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11860.8531023866726131No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8150.5862381493576556No Hit
GAGGAGGGGGACACATTTTTAAAAAATATAAATTGTATGTTCATTATAAAGAAATT6730.48409604235300885No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6520.46899051948612447No Hit
GAGGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5560.3999367006660816No Hit
GAGGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT5430.39058566270086753No Hit
GAGGAGGGGACACCTATGTCAACACACCAACTGGGGATGAGGATGGCAGAAGTCGC4330.31146149530290174No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.3085842528520666No Hit
GAGGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3980.2862856238580944No Hit
GAGGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3540.2546359568989081No Hit
GAGGAGGGGATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGA3200.23017939606680957No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2800.20140697155845835No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC2800.20140697155845835No Hit
GAGGAGGGGGCTCATTATATTCAAATATTCATTTTAGGAGCCATTCCGTAGTGCCA2760.19852972910762326No Hit
GAGGAGGGGGTCTCCTGCTTTAACTATTTCTAAAGTTCTTTTATCCATTTGTTCAC2450.17623110011365106No Hit
GAGGAGGGGGGTCACAACTGAAATCAGCATCCTGATTACTTTGTGTTCCAGTGTTC2440.1755117895009423No Hit
GAGGAGGGGACGATACCTTTTTCTTGGTGATTATGTGGACAGAGGTTATTTTAGTA2380.17119592582468962No Hit
GAGGAGGGGAACTATCTGTAAACCAACCTTTCAGTTGCTATATAACTTCCATAGAC2380.17119592582468962No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.15896764540864036No Hit
GAGGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2150.1546517817323877No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2120.15249384989426135No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGATCTTCCA2030.14602005437988233No Hit
GAGGAGGGGGACTTCCTGTCAGCGAGCTGAATCCCCTGGAGATGGGGGGGTGGGCT2010.14458143315446476No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1940.13954625886550331No Hit
GAGGAGGGGCCACCTTCCATGAGCAGCTTGCCCAGGCACTCCAGGTCTGAGAAGCA1940.13954625886550331No Hit
GAGGAGGGGAACCTAGGCTGGCCCTGAACTCAGGGCAGTCCTTCTGTCTCAGCTTC1820.13091453151299795No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.12947591028758038No Hit
GAGGAGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCC1800.12947591028758038No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCC1790.1287565996748716No Hit
GAGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1780.12803728906216283No Hit
GAGGAGGGGAGGTTGACTTTGAAATCGTAGAGCTCTACCTACTTTTACTTCCACAA1690.12156349354778381No Hit
GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1680.12084418293507501No Hit
GAGGAGGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCGGCCAAAGTGAACAACT1670.12012487232236624No Hit
GAGGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1640.11796694048423992No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAGCACCCGACTGCTCTTC1610.11580900864611357No Hit
GAGGAGGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1520.10933521313173454No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1520.10933521313173454No Hit
GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1520.10933521313173454No Hit
GAGGAGGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1520.10933521313173454No Hit
GAGGAGGGGATGGCAGGACATTTAAGTTCCGATACAAACACTAAGAAGAAAGTATA1510.10861590251902575No Hit
GAGGAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG1510.10861590251902575No Hit
GAGGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC1490.1071772812936082No Hit
GAGGAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1490.1071772812936082No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTAAAAAACATCGA1450.10430003884277309No Hit
GAGGAGGGGTGCTCCCCAACCACCCCTCCACTCCCTCAACACCTTAGCATTCTCCC1450.10430003884277309No Hit
GAGGAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA1420.10214210700464675No Hit
GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1410.10142279639193796No Hit
GAGGAGGGGAACTTCTTAAAAGACAAAAAAGAGAGAAATTGTATCCCCGTGCATGC1400.10070348577922918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA355.0484232E-850.24193250
AAATAAT150.00825634450.2419348
CATTGTT150.00825634450.2419346
TTATGCT150.00825634450.2419348
ACACATA150.00825634450.2419348
ATTGTTT150.00825634450.2419347
ATATGTG150.00825634450.2419349
TTATCTC204.0728343E-450.2419347
TCGGCAT150.00825634450.2419350
TAGTTGC150.00825634450.2419348
CTGAAAC150.00825634450.2419350
TGGTTCG150.00825634450.2419347
GAAAGTA204.0728343E-450.2419348
CCGTAGT204.0728343E-450.2419346
CGTAGTG204.0728343E-450.2419347
TTCCAAG150.00825634450.2419347
TTTATGC150.00825634450.2419347
GATCTGG150.00825634450.2419348
CACAACC150.00825634450.2419347
GTAGTTG150.00825634450.2419347
Produced by FastQC (version 0.11.5)