FastQCFastQC Report
Tue 31 May 2016
SRR212532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370742
Sequences flagged as poor quality0
Sequence length58
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT111753.0142255261071043No Hit
AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC49941.3470283917117565No Hit
AATAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT35090.9464803016653089No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA31120.8393977482993564No Hit
AATAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG27720.7476897680867017No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG24570.6627250217132129No Hit
AATAAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC23910.6449228843778153No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22860.616601302253319No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGG21240.5729051469755247No Hit
AATAAAGGGATGGTTATGGGTGAGCATGAGAAACCTCTCCTTCTGTGCCAGGCCGCAC15100.4072913238856132No Hit
AATAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC14090.38004865917538344No Hit
AATAAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT13790.3719567785683845No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12820.3457930312724212No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.3217871188049911No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10110.2726963764558642No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9970.26892016550593134No Hit
AATAAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT9130.2462628998063343No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.22792130376380343No Hit
AATAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG8000.21578348285330498No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.2036456619428066No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.18665271266810882No Hit
AATAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6690.1804489375360763No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6110.1648046350292117No Hit
AATAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5910.15941004795787908No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG5320.14349601609744783No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5310.1432262867438812No Hit
AATAAAGGGAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCACTC5260.14187763997604805No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5230.14106845191534814No Hit
AATAAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5210.14052899320821488No Hit
AATAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4940.13324630066191584No Hit
AATAAAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4860.13108846583338277No Hit
AATAAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4730.12758198423701655No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC4390.11841118621575113Illumina Single End Adapter 2 (96% over 25bp)
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4130.1113982230230187No Hit
AATAAAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTTC4080.11004957625518554No Hit
AATAAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA3890.10492471853741955No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG3870.10438525983028628No Hit
AATAAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3870.10438525983028628No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTCCG3810.10276688370888651No Hit
AATAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3750.10114850758748671No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT3720.10033931952678683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTA150.007167754752.08881852
GCCGTGG150.007167754752.08881852
ATCGCCA150.007167754752.08881852
TTAGGCT150.00717158852.0817851
TGACATT150.00717158852.0817851
CATGTGT150.00717158852.0817851
CACGTGC459.094947E-1152.0817851
GCTCTAC203.4202723E-452.0747450
TAGTAAT203.4202723E-452.0747450
ATTAGGC150.007175422352.07473850
TAATCGC150.007175422352.07473850
TGACATG150.00718309652.0606748
TCGTTGC203.427125E-452.0536446
GACGGCT203.427125E-452.0536446
CTACTCC150.007186935352.05363546
GAACTGA251.6442624E-552.04660444
TCTCGTT203.4294117E-452.04660444
CCGAAAT203.4294117E-452.04660445
GTTGAAT307.938579E-752.03957443
CGAACTG251.6455777E-552.03957443
Produced by FastQC (version 0.11.5)