##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212532_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 370742 Sequences flagged as poor quality 0 Sequence length 58 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.62168570056805 39.0 38.0 40.0 33.0 40.0 2 37.342672262651654 39.0 38.0 40.0 33.0 40.0 3 36.56867039612453 39.0 36.0 40.0 31.0 40.0 4 37.17141839877866 39.0 37.0 40.0 33.0 40.0 5 37.263377227290135 39.0 38.0 40.0 33.0 40.0 6 36.22871430806329 39.0 35.0 40.0 29.0 40.0 7 36.02434577145292 39.0 35.0 40.0 29.0 40.0 8 36.79974753332506 39.0 37.0 40.0 31.0 40.0 9 36.67288572646207 39.0 37.0 40.0 30.0 40.0 10 36.34201412302895 39.0 35.0 40.0 30.0 40.0 11 36.79688300759018 39.0 36.0 40.0 32.0 40.0 12 36.61913675817684 39.0 35.0 40.0 31.0 40.0 13 36.5265899196746 38.0 35.0 40.0 31.0 40.0 14 36.514031320972535 38.0 35.0 40.0 31.0 40.0 15 36.50459618818478 38.0 35.0 40.0 31.0 40.0 16 36.23044866780672 38.0 35.0 40.0 30.0 40.0 17 36.10502182110471 38.0 35.0 40.0 30.0 40.0 18 35.91537241531847 38.0 35.0 40.0 29.0 40.0 19 35.70033068818747 38.0 35.0 40.0 29.0 40.0 20 35.82500768728658 38.0 35.0 40.0 29.0 40.0 21 35.832398271574306 38.0 35.0 40.0 29.0 40.0 22 35.737529063337846 38.0 35.0 40.0 29.0 40.0 23 35.459753683154325 38.0 35.0 40.0 28.0 40.0 24 35.29495714000572 38.0 35.0 40.0 28.0 40.0 25 35.40259803313355 38.0 35.0 40.0 28.0 40.0 26 35.191426922226235 38.0 35.0 40.0 28.0 40.0 27 35.114257354170824 38.0 35.0 39.0 28.0 40.0 28 34.590693797843244 38.0 34.0 39.0 26.0 40.0 29 34.73033268418469 38.0 35.0 39.0 27.0 40.0 30 34.265656440327774 38.0 33.0 39.0 25.0 40.0 31 33.75425767784605 38.0 33.0 39.0 23.0 40.0 32 33.39409076932206 38.0 33.0 39.0 20.0 40.0 33 33.34459003835551 38.0 33.0 39.0 20.0 40.0 34 32.85221528718084 38.0 32.0 39.0 17.0 40.0 35 32.61894525033581 37.0 32.0 39.0 17.0 40.0 36 32.813843589342454 38.0 33.0 39.0 9.0 40.0 37 32.61987851389915 38.0 33.0 39.0 5.0 40.0 38 32.483479077094046 38.0 33.0 39.0 2.0 40.0 39 32.39395590464528 38.0 32.0 39.0 2.0 40.0 40 32.19352002200991 38.0 32.0 39.0 2.0 40.0 41 32.20578191842306 38.0 33.0 39.0 2.0 40.0 42 32.01076759579438 38.0 32.0 39.0 2.0 40.0 43 31.944149840050493 38.0 32.0 39.0 2.0 40.0 44 31.807197458070572 38.0 32.0 39.0 2.0 40.0 45 31.573514735314586 38.0 31.0 39.0 2.0 40.0 46 31.17700449369103 38.0 31.0 39.0 2.0 40.0 47 31.121755830199977 38.0 31.0 39.0 2.0 40.0 48 31.061619670822296 37.0 31.0 39.0 2.0 40.0 49 30.865049009823544 37.0 30.0 39.0 2.0 40.0 50 30.677473822766235 37.0 30.0 39.0 2.0 40.0 51 30.38390848622492 37.0 30.0 39.0 2.0 40.0 52 30.271477199777742 37.0 30.0 39.0 2.0 40.0 53 30.104387957123823 37.0 29.0 39.0 2.0 40.0 54 29.83231195818116 36.0 29.0 39.0 2.0 40.0 55 29.59889896477874 36.0 28.0 39.0 2.0 40.0 56 29.34583618796899 37.0 28.0 39.0 2.0 40.0 57 29.03591176613386 36.0 27.0 39.0 2.0 40.0 58 28.77006651525859 36.0 25.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -1.4309545714416743 1 2 -2.8871572552767333 1 3 -3.577214412001581 1 4 -2.0069308286950758 1 5 -2.561198495183014 1 6 -4.877318948292594 1 7 -4.240218784898687 1 8 -3.747037079399334 1 9 -4.020487181300183 1 10 -2.893105206370514 1 11 -2.5271950506840355 1 12 -2.1809834870271416 1 13 -2.9675477135414354 1 14 -2.1664372399525647 1 15 -2.241356442465886 1 16 -2.7041102691296146 1 17 -2.93325657681131 1 18 -2.577259886908955 1 19 -3.0283820568974775 1 20 -2.3668939359430965 1 21 -2.3017205698125807 1 22 -2.423764536479702 1 23 -2.580295445659509 1 24 -2.5032265211892835 1 25 -2.665629320483511 1 26 -2.01226372468426 1 27 -1.7725725408806738 1 28 -2.0620950483225613 1 29 -2.3381168277888165 1 30 -3.3088314677579262 1 31 -2.541304265433034 1 32 -2.3804015208658704 1 33 -2.837185114202505 1 34 -2.920731142389684 1 35 -3.0475061282297418 1 36 -2.91441504392926 1 37 -2.7598563229791075 1 38 -2.7673253792710533 1 39 -2.7046126914729953 1 40 -2.8577344590711284 1 41 -2.6487157526546525 1 42 -2.700126531590417 1 43 -2.787929635483515 1 44 -3.225340844756225 1 45 -2.4120655353159215 1 46 -2.525185082012765 1 47 -2.4929161265483195 1 48 -2.651481814870529 1 49 -2.113442379273682 1 50 -2.280213637522195 1 51 -2.5026624031545097 1 52 -2.4931171807636474 1 53 -3.1487183448662606 1 54 -3.0853492840758783 1 55 -2.7769255368307917 1 56 -2.4341199668027578 1 57 -2.559662098772197 1 58 -2.483420348872187 2 1 -0.8110536390407432 2 2 -1.7507728063922912 2 3 -2.187916210203383 2 4 -1.4535467128109616 2 5 -1.9211302301147484 2 6 -2.9989056948793404 2 7 -2.0346535168334228 2 8 -1.9782641856264362 2 9 -1.9105554463684484 2 10 -1.1070162952816034 2 11 -0.7580267190157031 2 12 -0.7755555815992352 2 13 -0.8898545733482948 2 14 -0.9983761343914637 2 15 -0.741460505069945 2 16 -0.7013422038615502 2 17 -0.8396210456757842 2 18 -0.9719901566392224 2 19 -0.9534153569307762 2 20 -0.6700907391399014 2 21 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117 14 -1.0459951820105076 117 15 -1.3902700288794705 117 16 -1.1834850610044043 117 17 -1.294978188532923 117 18 -1.418418728067799 117 19 -0.8046058331212507 117 20 -1.2296145486637116 117 21 -1.1194861375781429 117 22 -1.1684989687141396 117 23 -1.1325348728989368 117 24 -1.2369511299138978 117 25 -1.0069546618088552 117 26 -1.109021133941674 117 27 -1.3852307160388548 117 28 -1.1975637462912658 117 29 -1.2913094684814581 117 30 -1.794907891334347 117 31 -0.9746625737913419 117 32 -1.2429556334199816 117 33 -0.9612693632867533 117 34 -1.0556170897756303 117 35 -1.604990310713923 117 36 -0.6397730192872331 117 37 -0.6599978481206321 117 38 -0.8006670376127119 117 39 -0.7619928113531174 117 40 -0.5110811124177808 117 41 -0.7093218132607113 117 42 -0.7987154426793275 117 43 -0.857152079705962 117 44 -0.5235218304372111 117 45 -0.7356794214298077 117 46 -0.08533326716094791 117 47 -0.010960582092774729 117 48 -0.13674655013526404 117 49 -0.12158007491137468 117 50 -0.522325692134249 117 51 -0.26702304251514875 117 52 -0.3327150828615508 117 53 -0.11849856464647956 117 54 -0.5566488178754128 117 55 0.005208995303739528 117 56 -0.07296029314308328 117 57 0.40366623955614145 117 58 0.07362677067493095 118 1 -0.44600108648818804 118 2 -0.7390021071215855 118 3 -1.841310676097848 118 4 -0.8037451255093728 118 5 -1.2238436578281764 118 6 -1.8549224258960706 118 7 -1.5982796429595467 118 8 -1.3663486987109508 118 9 -1.5995837603967615 118 10 -0.6238009370662496 118 11 -0.030576054065043934 118 12 -0.20305450909815903 118 13 0.002117756124029313 118 14 -0.15568522920055727 118 15 -0.32884252995197016 118 16 -0.26046382548317126 118 17 -0.1550167985715376 118 18 -0.2661235757726459 118 19 -0.33013135864677423 118 20 -0.04954161859077999 118 21 -0.20839647648848825 118 22 -0.26783938055454826 118 23 0.13532226995820196 118 24 -0.310792828755595 118 25 -0.5434196982738868 118 26 0.048877836457300816 118 27 -0.02044628875442811 118 28 -0.4241564103839295 118 29 -0.3130008026727893 118 30 -0.2821719410983974 118 31 -0.6212198453486124 118 32 -0.96120960167395 118 33 -0.8393796163970038 118 34 -0.5757532974118362 118 35 -0.6637097444333548 118 36 -1.0178296473438628 118 37 -0.8058043689271557 118 38 -0.6303002422459159 118 39 -1.1957926826529892 118 40 -1.1670227683594376 118 41 -1.0877001916390903 118 42 -1.1856470171109024 118 43 -1.2602516078054897 118 44 -0.9941621135774952 118 45 -1.2432673665177525 118 46 -1.1702217270494089 118 47 -1.1200607536929468 118 48 -1.0733617367504493 118 49 -1.0722718381031378 118 50 -1.063482920791479 118 51 -0.7093761098682165 118 52 -1.1880078756543426 118 53 -0.865281061428977 118 54 -0.7422719409985348 118 55 -0.8681286280338831 118 56 -0.3160158486986404 118 57 -0.5495190886291859 118 58 -0.37159523704707453 119 1 -0.7789927607972302 119 2 -1.5313723216474884 119 3 -3.3317891785523983 119 4 -1.4098662166604043 119 5 -1.9516762808703803 119 6 -3.101807035056126 119 7 -2.450197429009066 119 8 -1.6613622751616575 119 9 -2.0910872399241924 119 10 -1.1830428136733957 119 11 -0.8443718858249483 119 12 -1.12357383081509 119 13 -1.2331144821022164 119 14 -1.5849708021987041 119 15 -1.533073707237044 119 16 -0.8474250381928883 119 17 -0.9095347896164725 119 18 -0.9748772396161272 119 19 -1.0531088271332294 119 20 -1.0071309444435812 119 21 -0.7883626702271371 119 22 -0.9371224360651453 119 23 -1.1121827200617531 119 24 -1.5115804427168626 119 25 -1.0341859135910028 119 26 -1.3683417783671104 119 27 -1.3174199090813374 119 28 -1.3831033813069453 119 29 -1.7847689780337817 119 30 -1.8064027587423936 119 31 -1.702813414966137 119 32 -1.7353280314810036 119 33 -2.0568995315217116 119 34 -1.4512365651106762 119 35 -1.6100872596731506 119 36 -1.0986409976162896 119 37 -1.0872504857026115 119 38 -1.0137765329078334 119 39 -0.8856561988638099 119 40 -1.2903796581833937 119 41 -0.9238926715441451 119 42 -0.8413266488384394 119 43 -1.0172960774248097 119 44 -0.8592967092510122 119 45 -0.4542957601808766 119 46 -0.8503389700409052 119 47 -1.0275880414597545 119 48 -1.0646764047118253 119 49 -1.5568702089290554 119 50 -1.8261288002038256 119 51 -0.9273096122328575 119 52 -1.1287480169932884 119 53 -0.8513899247833479 119 54 -1.066254606298564 119 55 -1.3976281874735612 119 56 -1.8822167801690313 119 57 -1.7189100364632495 119 58 -1.462818196533508 120 1 -1.2841014245885276 120 2 -1.8032411713606535 120 3 -2.7100815448687143 120 4 -1.6537839755482224 120 5 -2.1101495441340674 120 6 -3.618577689551337 120 7 -4.01294605762596 120 8 -3.386897219259474 120 9 -3.660347321160323 120 10 -3.0014968147621275 120 11 -2.4327894562784422 120 12 -2.534130340173995 120 13 -3.013002258995982 120 14 -2.7713323448476714 120 15 -2.9336641347735792 120 16 -3.375438940458281 120 17 -2.954235597790337 120 18 -2.874462684111755 120 19 -3.1262841547995706 120 20 -3.17458624363541 120 21 -3.112909381001387 120 22 -3.025163137878309 120 23 -2.957917823281889 120 24 -3.6186111365739 120 25 -3.284510439364631 120 26 -2.973802186222727 120 27 -3.3879571562652906 120 28 -2.548109034336548 120 29 -2.95000493967693 120 30 -2.896244055170513 120 31 -2.2965490206777908 120 32 -2.5412406817050304 120 33 -2.0749473519647417 120 34 -2.2424094640680075 120 35 -1.7048487855723984 120 36 -1.4039255334397502 120 37 -1.602513665636451 120 38 -1.5855071974528698 120 39 -1.4878294746897787 120 40 -1.4486435499802184 120 41 -1.7466178505567491 120 42 -2.000825832289717 120 43 -2.060656908210788 120 44 -1.9386275580429384 120 45 -1.7337438569942414 120 46 -1.9132969701246516 120 47 -1.5873217209539128 120 48 -1.906726570115282 120 49 -2.218337484168785 120 50 -1.9969968543054115 120 51 -1.9711938716859798 120 52 -1.9756346632811308 120 53 -1.5438232399711538 120 54 -1.791642990369585 120 55 -1.581121341026595 120 56 -1.7837703164531078 120 57 -1.5876341267442236 120 58 -1.472930838382677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1115.0 3 124.0 4 286.0 5 209.0 6 434.0 7 868.0 8 753.0 9 1142.0 10 1254.0 11 1196.0 12 1647.0 13 1952.0 14 2110.0 15 2385.0 16 2485.0 17 2604.0 18 2931.0 19 3175.0 20 3754.0 21 3397.0 22 4034.0 23 4550.0 24 4483.0 25 4472.0 26 4996.0 27 5397.0 28 5837.0 29 7136.0 30 8549.0 31 10007.0 32 12186.0 33 15003.0 34 18120.0 35 22409.0 36 30065.0 37 45365.0 38 62183.0 39 71700.0 40 429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 45.933020806922336 36.260795917376505 6.494003916470213 11.312179359230948 11 30.420615953951806 35.0070938819988 13.153082197323208 21.419207966726187 12 47.285983244412556 17.39484601151205 20.67448522152872 14.644685522546677 13 23.220730319197717 20.11290870740299 25.381262441266433 31.28509853213286 14 20.47488549988941 34.84552599921239 28.54923369890652 16.130354801991682 15 33.30132545004343 21.135182957420522 29.545344201628087 16.018147390907963 16 23.239134598007773 22.51167256925994 35.172802576461734 19.07639025627055 17 35.45925592259825 21.16598919461295 19.880455628053006 23.494299254735786 18 33.90120353237561 22.70177104293552 22.589293902498234 20.807731522190632 19 22.482453916242736 26.81084755271917 32.54697387373293 18.15972465730516 20 23.3601273163758 36.78903784425323 21.42044075202978 18.430394087341192 21 23.288502621868325 25.732073110204784 32.482305086208754 18.497119181718134 22 26.251550952149756 26.140691589793384 21.53935372498247 26.06840373307439 23 26.756380585541116 36.608727551290144 21.832634719232658 14.802257143936085 24 24.76877388797031 37.226257683935685 22.859084913726836 15.145883514367172 25 23.82444887401178 27.780849703701506 20.358147601694984 28.036553820591735 26 29.954164452226085 25.97842303054174 23.949410936323932 20.118001580908242 27 18.2248297255378 25.475217479263605 26.43280059343179 29.867152201766807 28 21.20280142877184 37.08062201215104 24.966007316520443 16.750569242556683 29 31.097125067779558 25.774305546188565 26.429305586653967 16.69926379937791 30 20.55228133803957 33.20542296773637 29.96367777381993 16.27861792040413 31 32.01475441242738 23.755467470406177 30.210927655349014 14.018850461817426 32 27.43044128040918 21.97742612505193 38.380982697319084 12.21114989721981 33 32.02074886908953 20.778001667030825 30.950935884052345 16.250313579827306 34 25.323049697048177 21.829796647297172 36.366628359312195 16.480525296342456 35 19.334353089077553 34.34472600134349 36.40878716510601 9.91213374447295 36 23.613914282322213 29.76805931810087 33.277929478808026 13.34009692076889 37 20.577782934733627 28.007037445795746 39.24860155050474 12.16657806896589 38 23.05460492934178 27.917282924354144 36.904209264917036 12.123902881387039 39 20.92241930652591 27.20923768582909 35.55154370710988 16.316799300535124 40 20.567701263267548 26.13865447568055 39.52346126132447 13.770182999727428 41 18.916220264198298 28.555545060787196 39.0182294126378 13.510005262376707 42 17.25284722053508 29.62540052746802 37.97148865295898 15.150263599037924 43 18.02339234957337 23.86149224079058 40.445334621811085 17.669780787824966 44 16.36315550853861 25.972063300157604 39.097076793540445 18.567704397763336 45 19.349093264248705 27.787402849740932 37.85810664939551 15.005397236614854 46 18.517488934470475 29.487207168304003 38.99600561373205 12.999298283493468 47 21.117648488317776 26.149118804155 38.31494077208255 14.418291935444675 48 21.730350243926143 24.726308656340866 38.88914386917891 14.654197230554079 49 20.78437620676898 25.157774495216135 38.5501759351025 15.507673362912394 50 14.866985149758166 27.317801656508156 39.616037937115344 18.199175256618336 51 15.041712789222172 27.397607926779884 41.79756472906936 15.76311455492859 52 17.231689507824118 25.984122313790802 42.27265242659742 14.511535751787658 53 13.134152631394183 26.38874261781123 42.59296764663788 17.884137104156707 54 13.86257332051462 28.590679787843108 42.16240156417098 15.384345327471294 55 17.004729914072776 26.40648087369698 39.26666072388485 17.322128488345395 56 14.068616723806745 28.245569955395617 37.325304004128135 20.360509316669503 57 17.857384050591193 27.77761269426203 37.63030717239852 16.73469608274826 58 18.65348246609391 29.31161182255363 36.1276814178419 15.907224293510563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 626.0 5 1252.0 6 1560.5 7 1869.0 8 2781.5 9 3369.0 10 3044.0 11 3646.0 12 4248.0 13 4621.5 14 4995.0 15 5365.5 16 6304.0 17 6872.0 18 8003.0 19 9134.0 20 10865.0 21 12596.0 22 13576.5 23 15178.5 24 15800.0 25 15116.5 26 14433.0 27 13125.0 28 16692.5 29 21568.0 30 15851.0 31 10134.0 32 8994.5 33 7855.0 34 7417.0 35 6967.5 36 6956.0 37 7499.0 38 8042.0 39 8514.5 40 8987.0 41 10047.0 42 10569.5 43 10032.0 44 10991.5 45 11951.0 46 13594.5 47 16076.0 48 16914.0 49 15775.5 50 14637.0 51 14562.5 52 14488.0 53 14088.5 54 15975.5 55 18262.0 56 18820.0 57 19378.0 58 20784.5 59 15819.0 60 9447.0 61 7058.0 62 4669.0 63 3943.5 64 3218.0 65 2577.0 66 1682.5 67 1429.0 68 1136.5 69 844.0 70 570.5 71 297.0 72 253.0 73 156.5 74 104.0 75 85.5 76 67.0 77 54.5 78 28.5 79 15.0 80 9.5 81 4.0 82 4.5 83 5.0 84 2.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.6972935356663123E-4 17 2.6972935356663123E-4 18 0.0 19 0.001078917414266525 20 0.003236752242799575 21 0.0037762109499328376 22 5.394587071332625E-4 23 0.00215783482853305 24 2.6972935356663123E-4 25 2.6972935356663123E-4 26 0.018611325396097556 27 0.004585399010632731 28 0.019959972163930712 29 0.014835114446164716 30 0.04585399010632731 31 0.038031838852895 32 0.0129470089711983 33 0.0062037751320325185 34 0.01510484379973135 35 0.01753240798183103 36 0.03506481596366206 37 0.041268591095694585 38 0.060958833906058656 39 0.046663178167027204 40 0.05259722394549309 41 0.05043938911696005 42 0.07903070059502296 43 0.07525448964509011 44 0.05124857717765994 45 0.04909074234912688 46 0.05987991649179213 47 0.10600363595168608 48 0.09251716827335452 49 0.11733226880148459 50 0.1200295623371509 51 0.09494473245545419 52 0.07687286576648991 53 0.11463497526581828 54 0.12137820910498406 55 0.14700249769381404 56 0.16156788278641213 57 0.15077870864374687 58 0.162377070847112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 370742.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.77215674979585 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68499340764423 27.832411211012115 2 11.69861151798867 8.837635760248705 3 5.1151508085954545 5.796308344233376 4 2.6774388529497832 4.045305601664513 5 1.522524402906854 2.875451520099351 6 0.9742070122882394 2.2078739984941 7 0.7378599007620641 1.9509391881681444 8 0.5069302890807045 1.5318280272300553 9 0.4338180435748558 1.4747618828519298 >10 2.3349467244197784 15.698943431393197 >50 0.16463328573414887 4.347850925913954 >100 0.12812764411483762 9.311609096976342 >500 0.010021156522948193 2.57015551904347 >1k 0.010021156522948193 8.49751923080341 >5k 0.0 0.0 >10k+ 7.157968944962995E-4 3.021406261867323 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 11175 3.0142255261071043 No Hit AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 4994 1.3470283917117565 No Hit AATAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 3509 0.9464803016653089 No Hit AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 3112 0.8393977482993564 No Hit AATAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 2772 0.7476897680867017 No Hit AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 2457 0.6627250217132129 No Hit AATAAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 2391 0.6449228843778153 No Hit AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2286 0.616601302253319 No Hit AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGG 2124 0.5729051469755247 No Hit AATAAAGGGATGGTTATGGGTGAGCATGAGAAACCTCTCCTTCTGTGCCAGGCCGCAC 1510 0.4072913238856132 No Hit AATAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1409 0.38004865917538344 No Hit AATAAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 1379 0.3719567785683845 No Hit AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1282 0.3457930312724212 No Hit AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1193 0.3217871188049911 No Hit AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1011 0.2726963764558642 No Hit AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 997 0.26892016550593134 No Hit AATAAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 913 0.2462628998063343 No Hit AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.22792130376380343 No Hit AATAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 800 0.21578348285330498 No Hit AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.2036456619428066 No Hit AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.18665271266810882 No Hit AATAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 669 0.1804489375360763 No Hit AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 611 0.1648046350292117 No Hit AATAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 591 0.15941004795787908 No Hit AATAAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG 532 0.14349601609744783 No Hit AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 531 0.1432262867438812 No Hit AATAAAGGGAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCACTC 526 0.14187763997604805 No Hit AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 523 0.14106845191534814 No Hit AATAAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 521 0.14052899320821488 No Hit AATAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 494 0.13324630066191584 No Hit AATAAAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 486 0.13108846583338277 No Hit AATAAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 473 0.12758198423701655 No Hit AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 439 0.11841118621575113 Illumina Single End Adapter 2 (96% over 25bp) AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 413 0.1113982230230187 No Hit AATAAAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTTC 408 0.11004957625518554 No Hit AATAAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA 389 0.10492471853741955 No Hit AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG 387 0.10438525983028628 No Hit AATAAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 387 0.10438525983028628 No Hit AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTCCG 381 0.10276688370888651 No Hit AATAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 375 0.10114850758748671 No Hit AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT 372 0.10033931952678683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.039650214974294794 0.0 0.0 0.0 0.0 11 0.08658312249488863 0.0 0.0 0.0 0.0 12 0.09116852150552136 0.0 0.0 0.0 0.0 13 0.09332635633405441 0.0 0.0 0.0 0.0 14 0.11975983298358427 0.0 0.0 0.0 0.0 15 0.12461496134778363 0.0 0.0 0.0 0.0 16 0.12731225488344994 0.0 0.0 0.0 0.0 17 0.12973981906554963 0.0 0.0 0.0 0.0 18 0.1337857593690491 0.0 0.0 0.0 0.0 19 0.13675278225828205 0.0 0.0 0.0 0.0 20 0.15941004795787905 0.0 0.0 0.0 0.0 21 0.1621073414935454 0.0 0.0 0.0 0.0 22 0.16804138727201126 0.0 0.0 0.0 0.0 23 0.18260677236460934 0.0 0.0 0.0 0.0 24 0.19636296939650755 0.0 0.0 0.0 0.0 25 0.2047245793570731 0.0 0.0 0.0 0.0 26 0.2176715883282714 0.0 0.0 0.0 0.0 27 0.22819103311737002 0.0 0.0 0.0 0.0 28 0.30668227500525974 0.0 0.0 0.0 0.0 29 0.314504426258692 0.0 0.0 0.0 0.0 30 0.350378430283054 0.0 0.0 0.0 0.0 31 0.35766112282935303 0.0 0.0 0.0 0.0 32 0.36494381537565207 0.0 0.0 0.0 0.0 33 0.37006867309341807 0.0 0.0 0.0 0.0 34 0.3762724482254506 0.0 0.0 0.0 0.0 35 0.48443391900566973 0.0 0.0 0.0 0.0 36 0.4938744463805018 0.0 0.0 0.0 0.0 37 0.500347950866101 0.0 0.0 0.0 0.0 38 0.5062819966445669 0.0 0.0 0.0 0.0 39 0.5122160424230328 0.0 0.0 0.0 0.0 40 0.5246235926870978 0.0 0.0 0.0 0.0 41 0.5313668265262635 0.0 0.0 0.0 0.0 42 0.5378403310118627 0.0 0.0 0.0 0.0 43 0.5448532942045952 0.0 0.0 0.0 0.0 44 0.5526754454580274 0.0 0.0 0.0 0.0 45 0.561037055418593 0.0 0.0 0.0 0.0 46 0.5691289360255919 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCTA 15 0.0071677547 52.088818 52 GCCGTGG 15 0.0071677547 52.088818 52 ATCGCCA 15 0.0071677547 52.088818 52 TTAGGCT 15 0.007171588 52.08178 51 TGACATT 15 0.007171588 52.08178 51 CATGTGT 15 0.007171588 52.08178 51 CACGTGC 45 9.094947E-11 52.08178 51 GCTCTAC 20 3.4202723E-4 52.07474 50 TAGTAAT 20 3.4202723E-4 52.07474 50 ATTAGGC 15 0.0071754223 52.074738 50 TAATCGC 15 0.0071754223 52.074738 50 TGACATG 15 0.007183096 52.06067 48 TCGTTGC 20 3.427125E-4 52.05364 46 GACGGCT 20 3.427125E-4 52.05364 46 CTACTCC 15 0.0071869353 52.053635 46 GAACTGA 25 1.6442624E-5 52.046604 44 TCTCGTT 20 3.4294117E-4 52.046604 44 CCGAAAT 20 3.4294117E-4 52.046604 45 GTTGAAT 30 7.938579E-7 52.039574 43 CGAACTG 25 1.6455777E-5 52.039574 43 >>END_MODULE