FastQCFastQC Report
Tue 31 May 2016
SRR212530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373957
Sequences flagged as poor quality0
Sequence length58
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT97212.5994967335816686No Hit
AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC45631.2201937655933703No Hit
AATAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT31300.836994627724578No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA27350.7313675101682814No Hit
AATAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG24690.6602363373329019No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGG22600.6043475586765323No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG21130.5650382263201384No Hit
AATAAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC20440.5465869070508106No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18980.5075449851186099No Hit
AATAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC12610.3372045449075696No Hit
AATAAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT12280.32838000090919545No Hit
AATAAAGGGATGGTTATGGGTGAGCATGAGAAACCTCTCCTTCTGTGCCAGGCCGCAC12200.3262407175156502No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10800.28880325812860835No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.28505951218990416No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.24040197134964714No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8170.2184743165658084No Hit
AATAAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7520.20109263899325325No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.18531542396585704No Hit
AATAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6790.18157167802715285No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.17675829039167604No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.1722123131803924No Hit
AATAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6260.1673989255449156No Hit
AATAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5490.1468083228820426No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5200.13905342058044107No Hit
AATAAAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4840.12942664530948747No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.1254154889465901No Hit
AATAAAGGGAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCACTC4490.12006728046272701No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG4440.11873022834176122No Hit
AATAAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4280.11445166155467074No Hit
AATAAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4260.1139168407062844No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.11204496773693233No Hit
AATAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4170.111510146888546No Hit
AATAAAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTTC3990.1066967592530692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT150.007048320552.3106610
GGACTAA203.3448072E-452.310668
GAGAGTA251.5957508E-552.310669
AGTGAAT203.3448072E-452.3106610
ACTAATC150.007048320552.3106610
ATGACAG150.007048320552.3106610
GCGGCAA203.3448072E-452.310669
GACTAAT150.007048320552.310669
CTCGTAA459.094947E-1152.1067439
CCGCGTT150.007158047552.10673539
AGCTACG251.6330805E-552.1067338
TTGACTA203.4303602E-452.04377437
CATAAGC150.00719235852.0437737
TCTTACG150.00719999952.02979752
GTCACTC600.052.02979752
CAATGCG150.00719999952.02979752
CCTATTA251.6474281E-552.02979752
TAATATT150.00720382252.02281635
TTAGGCG150.00720382252.02281635
CAATTGC203.4394662E-452.0158444
Produced by FastQC (version 0.11.5)