FastQCFastQC Report
Tue 31 May 2016
SRR212529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340629
Sequences flagged as poor quality0
Sequence length56
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT112083.290383378984173No Hit
AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC36141.060978366492577No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC35451.040721723634805No Hit
AATAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC25960.7621194907068981No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC25230.7406885497124437No Hit
AATAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA22050.6473318478461905No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20880.6129836273482293No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGT19520.5730574907010266No Hit
AATAAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA18170.5334249285879946No Hit
AATAAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC15500.4550405279644423No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.35170229193638824No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11520.3381978633645403No Hit
AATAAAGGGATGGTTATGGGTGAGCATGAGAAACCTCTCCTTCTGTGCCAGGCCGC10510.3085468354133089No Hit
AATAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10340.30355606833240856No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9770.28682231988468393No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.259813462740988No Hit
AATAAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8830.2592263136726468No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.25394197205757585No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.20902506832947282No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.20315357764606068No Hit
AATAAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6580.19317204348425998No Hit
AATAAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA6140.18025476398075324No Hit
AATAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6060.1779061677073884No Hit
AATAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5580.16381459006719923No Hit
AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC5470.16058527019132254No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.14678726708530396No Hit
AATAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4820.14150292547023302No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC4510.13240211491094417No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.1306406677059205No Hit
AATAAAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4230.12418202795416715No Hit
AATAAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4120.12095270807829046No Hit
AATAAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4020.11801696273658438No Hit
AATAAAGGGAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCAC3790.1112647484506604No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3680.1080354285747837No Hit
AATAAAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3670.1077418540406131No Hit
AATAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3650.10715470497227189No Hit
AATAAAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3470.10187036335720094No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC3450.10128321428885972No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG3410.10010891615217729Illumina Single End Adapter 2 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG150.00819593350.35124248
GTCCGCG150.00819593350.35124249
TAGGCCG309.963787E-750.35124250
CGTCCGC150.00819593350.35124248
GATTAGG150.00819593350.35124249
TTATCTC252.013856E-550.2915447
TGATGTA150.00823446550.2915447
ACGGCTT150.0082392950.28408446
GCGTGAC150.00825861550.25429545
CGAAAGG204.0793302E-450.24685742
CGTAGCG150.00826345150.24685342
AATCTCC150.00826345150.24685343
GCCACGT252.0244759E-550.24685342
CCGCATT252.0244759E-550.24685343
GCGTAGC150.00826345150.24685344
CGAATCT150.00828281850.2171141
AATAGCG150.00828281850.2171141
AGACTAC150.00831679150.16514240
TATTGTT150.00831679150.16514239
CTGTTAC150.00831679150.16514239
Produced by FastQC (version 0.11.5)