SRR212528_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212528_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302871
Sequences flagged as poor quality	0
Sequence length	56
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	9490	3.1333472006233674	No Hit
AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	3686	1.2170197873021849	No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	3034	1.0017466181971861	No Hit
AATAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	2597	0.8574607671252777	No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	2205	0.7280327268044811	No Hit
AATAAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	2181	0.7201085610705548	No Hit
AATAAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	2158	0.7125145689088754	No Hit
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1882	0.6213866629687226	No Hit
AATAAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1693	0.5589838578140528	No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGT	1255	0.41436783316989745	No Hit
AATAAAGGGATGGTTATGGGTGAGCATGAGAAACCTCTCCTTCTGTGCCAGGCCGC	1210	0.3995100224187855	No Hit
AATAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	1019	0.3364468701196219	No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.33380548154164646	No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	995	0.3285227043856956	No Hit
AATAAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	924	0.3050803807561635	No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	824	0.2720630235314705	No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	817	0.269751808525742	No Hit
AATAAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	799	0.26380868422529724	No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	692	0.2284801119948757	No Hit
AATAAAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	652	0.21527316910499847	No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.20933004480455375	No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	594	0.19612310191467652	No Hit
AATAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	556	0.18357650616929322	No Hit
AATAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	554	0.18291615902479935	No Hit
AATAAAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	536	0.1769730347243546	No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	498	0.16442643897897125	No Hit
AATAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	456	0.15055914894460015	No Hit
AATAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	438	0.14461602464415543	No Hit
AATAAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	405	0.13372029676000674	No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	400	0.13206942889877207	No Hit
AATAAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	388	0.12810734603180893	No Hit
AATAAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC	381	0.12579613102608042	No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	371	0.1224943953036111	Illumina Single End Adapter 2 (95% over 23bp)
AATAAAGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	362	0.11952283315338874	No Hit
AATAAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	361	0.11919265958114181	No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	333	0.10994779955822775	No Hit
AATAAAGGGAGAGCAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGTCAC	324	0.1069762374080054	No Hit
AATAAAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	323	0.10664606383575846	No Hit
AATAAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	316	0.10433484883002994	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGAC	15	0.008241933	50.27865	50
TAGTAAT	35	5.0460585E-8	50.27865	50
ATAGTAA	35	5.0527888E-8	50.270275	49
TGCACGT	15	0.008247361	50.270275	49
CTAATCG	15	0.008247361	50.270275	49
CGATGCA	15	0.008252792	50.2619	48
TATTTGT	20	4.0759292E-4	50.25354	46
GTAGCCT	30	1.0094445E-6	50.25354	46
ACATCCC	20	4.0759292E-4	50.25354	46
CATCCCC	20	4.0759292E-4	50.25354	47
ATTCTAA	15	0.008258225	50.25354	46
CAAGTAT	15	0.0082691	50.23681	45
AACCCGT	15	0.0082691	50.23681	44
TTTAGAT	20	4.0893423E-4	50.220097	43
CCGATTG	25	2.0303267E-5	50.220097	43
TAACGGG	20	4.0893423E-4	50.220097	43
ATAACGG	20	4.0893423E-4	50.220097	42
CCACGTG	25	2.0303267E-5	50.220097	43
GCCACAT	20	4.0893423E-4	50.220097	43
TCGAACT	20	4.0893423E-4	50.220097	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content