FastQCFastQC Report
Tue 31 May 2016
SRR212526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66929
Sequences flagged as poor quality0
Sequence length58
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13261.9812039624079247No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13241.9782157211373246No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA12751.9050038100076199No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10251.5314736511825964No Hit
AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7931.1848376637929747No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7561.1295552002868712No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6791.0145079113687638No Hit
AAGCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5030.7515426795559473No Hit
AAGCGGGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4970.7425779557441468No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA4550.6798248890615428No Hit
AAGCGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3910.5842011684023368No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3630.5423657906139342No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3360.5020245334608316No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2450.36605955564852305No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2380.35560071120142245No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2050.3062947302365193No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2020.301812368330619No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1970.29434176515411853No Hit
AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG1880.2808946794364177No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1830.2734240762599172No Hit
AAGCGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1790.26744759371871685No Hit
AAGCGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1790.26744759371871685No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1710.25549462863631606No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.23457693974211477No Hit
AAGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.22710633656561433No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA1500.22411809529501414No Hit
AAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1470.21963573338911382No Hit
AAGCGGGGGCCTGAAGAGGTCACTGAAGAGTAAGGTCCCTGGAGGAGTCAGTAGGGAA1450.21664749211851367No Hit
AAGCGGGGGGAATTTATTTTGTTATACTCTAGCCTAGTAATAATTGGCAATTGGTTCC1430.21365925084791348No Hit
AAGCGGGGGCGGCAGTCTCTGGTCTCACTATTCTTTCTGAGTGCTACGTGGGACTGAG1420.21216513021261335No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1340.20021216513021264No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAAC1320.19722392385961243No Hit
AAGCGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1300.19423568258901225No Hit
AAGCGGGGGAGTTGTGTAATATTTTGTCCTTTGTAACTATGTAAACATTGTCTATTGG1290.19274156195371214No Hit
AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1280.19124744131841206No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA1270.18975332068311196No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1260.18825920004781185No Hit
AAGCGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1250.18676507941251178No Hit
AAGCGGGGGCATTTGTGTAGTTTACCACTAAAGGAGTTTGGATAAGAATATGTGCATC1250.18676507941251178No Hit
AAGCGGGGGATCTTCATCTGTCTAAGGATATCTTAATTAGTAGACCAACAAAGATCTT1250.18676507941251178No Hit
AAGCGGGGGGGGAATGCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1250.18676507941251178No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1230.18377683814191156No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1200.1792944762360113No Hit
AAGCGGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1170.174812114330111No Hit
AAGCGGGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCAGA1160.17331799369481093No Hit
AAGCGGGGGAGTTCTGTGAATTGAGCTACACAGCGTGATGAGATGCGCGTGCCTGGTG1160.17331799369481093No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCTCTTCCGA1140.17032975242421072No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG1140.17032975242421072No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCA1100.16435326988301036No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1090.16285914924771028No Hit
AAGCGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1080.16136502861241017No Hit
AAGCGGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC1060.15837678734181No Hit
AAGCGGGGGGTCACCAGCGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCGCG1050.15688266670650988No Hit
AAGCGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTCGA1020.1524003048006096No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTACTCTTCCGA1000.1494120635300094No Hit
AAGCGGGGGGAGCAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAGACC1000.1494120635300094No Hit
AAGCGGGGGGGCACAGGGCAAAGATGGCGGCGGAGGAGCAGGAGGCGTTGAGGGAGTT990.14791794289470933No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG990.14791794289470933No Hit
AAGCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA990.14791794289470933No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTCCA980.14642382225940923No Hit
AAGCGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCCA960.14343558098880904No Hit
AAGCGGGGGCAGCCAAACTACTGTCTTCTGGATCATATCCAGCCCAACTCCATTGCTC960.14343558098880904No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT940.14044733971820886No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC940.14044733971820886No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA940.14044733971820886No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGTTCTTCCGA940.14044733971820886No Hit
AAGCGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT930.13895321908290875No Hit
AAGCGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG930.13895321908290875No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA910.13596497781230857No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG910.13596497781230857No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTCTG880.1314826159064083No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA850.12700025400050802No Hit
AAGCGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG840.1255061333652079No Hit
AAGCGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA830.1240120127299078No Hit
AAGCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACTGACTGCTCTTCTGA830.1240120127299078No Hit
AAGCGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT830.1240120127299078No Hit
AAGCGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT820.12251789209460771No Hit
AAGCGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.12251789209460771No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA820.12251789209460771No Hit
AAGCGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT810.12102377145930762No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC810.12102377145930762No Hit
AAGCGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA800.11952965082400752No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCGAA800.11952965082400752No Hit
AAGCGGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG780.11654140955340735No Hit
AAGCGGGGGGGAGCCATGATTGGAACTCTTTGGCTTGGCTTCATTCTTCTCTCGCCCG770.11504728891810725No Hit
AAGCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGA750.11205904764750707No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA740.11056492701220696No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA740.11056492701220696No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA740.11056492701220696No Hit
AAGCGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC730.10907080637690687No Hit
AAGCGGGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA720.10757668574160678No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCGGA720.10757668574160678No Hit
AAGCGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC700.10458844447100658No Hit
AAGCGGGGGATCACACACAGAACAAGCCCTGTGAGAAGCAGAATCCACAATGAGGGTC700.10458844447100658No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT690.10309432383570649No Hit
AAGCGGGGGATGCCAGCCTAGGAAGAGCTCTTCTTCAGCAGTGTTTCTGCCTCGGACC690.10309432383570649No Hit
AAGCGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGT680.10160020320040639No Hit
AAGCGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG680.10160020320040639No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA670.10010608256510632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCA150.007159539552.0568927
ACTAAAG203.4057524E-452.0568927
AGCTCGA203.4057524E-452.0568952
TGCGTAA150.007159539552.0568927
CAGTAGC150.007159539552.0568927
CCGCTCC401.8317223E-952.0568952
TGCTGCT150.007159539552.0568922
TAAGATA150.007159539552.0568921
TGCTGAT150.007159539552.0568951
GATCGCC203.4057524E-452.0568921
CTAAGGA150.007159539552.0568922
ACTCTAG150.007159539552.0568926
CTTAGCA150.007159539552.0568927
AAGGGAT150.007159539552.0568921
AGGGATC150.007159539552.0568922
CTCTAGC150.007159539552.0568927
GAGTTAC150.007159539552.0568952
CTGCGTA150.007159539552.0568926
CCACTAA203.4057524E-452.0568925
GCAAAGG150.007159539552.0568952
Produced by FastQC (version 0.11.5)