FastQCFastQC Report
Tue 31 May 2016
SRR212525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66101
Sequences flagged as poor quality0
Sequence length58
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT12621.9091995582517662No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC12211.847173265154839No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11571.7503517344669521No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9911.4992208892452459No Hit
AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7891.1936279330116035No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6460.9772923253808565No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6260.9470355970408919No Hit
AAGCGGGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4800.7261614801591504No Hit
AAGCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4400.6656480234792211No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA3930.5945447118803044No Hit
AAGCGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3460.5234414002813875No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3020.45687659793346547No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2790.4220813603425062No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC2640.39938881408753274No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2570.3887989591685451No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2030.3071057926506407No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1930.2919774284806584No Hit
AAGCGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1830.2768490643106761No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1730.26172070014069376No Hit
AAGCGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1710.25869502730669736No Hit
AAGCGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1710.25869502730669736No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1640.24810517238770974No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1630.24659233597071148No Hit
AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG1450.21936128046474335No Hit
AAGCGGGGGCGGCAGTCTCTGGTCTCACTATTCTTTCTGAGTGCTACGTGGGACTGAG1420.21482277121374865No Hit
AAGCGGGGGGAATTTATTTTGTTATACTCTAGCCTAGTAATAATTGGCAATTGGTTCC1350.20423291629476106No Hit
AAGCGGGGGCCTGAAGAGGTCACTGAAGAGTAAGGTCCCTGGAGGAGTCAGTAGGGAA1330.2012072434607646No Hit
AAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1300.1966687342097699No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA1300.1966687342097699No Hit
AAGCGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1290.19515589779277165No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1280.19364306137577345No Hit
AAGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.19213022495877521No Hit
AAGCGGGGGATCTTCATCTGTCTAAGGATATCTTAATTAGTAGACCAACAAAGATCTT1240.18759171570778052No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1240.18759171570778052No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1170.17700186078879293No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAAC1160.17548902437179467No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1130.1709505151208No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCA1130.1709505151208No Hit
AAGCGGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1100.16641200586980529No Hit
AAGCGGGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCAGA1100.16641200586980529No Hit
AAGCGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1100.16641200586980529No Hit
AAGCGGGGGCATTTGTGTAGTTTACCACTAAAGGAGTTTGGATAAGAATATGTGCATC1090.16489916945280708No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG1090.16489916945280708No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTCCA1080.16338633303580885No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA1050.15884782378481416No Hit
AAGCGGGGGAGTTCTGTGAATTGAGCTACACAGCGTGATGAGATGCGCGTGCCTGGTG1040.15733498736781593No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAA1020.15430931453381946No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1000.151283641699823No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1000.151283641699823No Hit
AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG990.14977080528282477No Hit
AAGCGGGGGGGGAATGCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC990.14977080528282477No Hit
AAGCGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA970.1467451324488283No Hit
AAGCGGGGGAGTTGTGTAATATTTTGTCCTTTGTAACTATGTAAACATTGTCTATTGG970.1467451324488283No Hit
AAGCGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCCA920.13918095036383715No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT920.13918095036383715No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.13918095036383715No Hit
AAGCGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT920.13918095036383715No Hit
AAGCGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA910.13766811394683892No Hit
AAGCGGGGGCAGCCAAACTACTGTCTTCTGGATCATATCCAGCCCAACTCCATTGCTC910.13766811394683892No Hit
AAGCGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA900.1361552775298407No Hit
AAGCGGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC890.13464244111284246No Hit
AAGCGGGGGGAGCAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAGACC880.13312960469584423No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCTCTTCCGA880.13312960469584423No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTACTCTTCCGA870.13161676827884602No Hit
AAGCGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT870.13161676827884602No Hit
AAGCGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT860.1301039318618478No Hit
AAGCGGGGGGGCACAGGGCAAAGATGGCGGCGGAGGAGCAGGAGGCGTTGAGGGAGTT840.1270782590278513No Hit
AAGCGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTCGA780.11800124052586194No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG760.11497556769186547No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGTTCTTCCGA760.11497556769186547No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA750.11346273127486725No Hit
AAGCGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.11346273127486725No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA740.11194989485786902No Hit
AAGCGGGGGGGAGCCATGATTGGAACTCTTTGGCTTGGCTTCATTCTTCTCTCGCCCG740.11194989485786902No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA740.11194989485786902No Hit
AAGCGGGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT730.1104370584408708No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG720.10892422202387256No Hit
AAGCGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT710.10741138560687433No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA710.10741138560687433No Hit
AAGCGGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCCTCG700.1058985491898761No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTCTG700.1058985491898761No Hit
AAGCGGGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA700.1058985491898761No Hit
AAGCGGGGGGTCACCAGCGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCGCG700.1058985491898761No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC690.10438571277287788No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCGAA690.10438571277287788No Hit
AAGCGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC680.10287287635587963No Hit
AAGCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACTGACTGCTCTTCTGA680.10287287635587963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAATC203.30623E-452.36898410
GGGCATG150.006991670552.3689847
GGTCTTT203.30623E-452.3689848
GGACTGG251.5708316E-552.3689848
GGACTAA203.30623E-452.3689848
GAGTTCT150.006991670552.3689849
AACCATA150.006991670552.36898410
GACTGGT150.006991670552.3689849
GGGATGC251.5708316E-552.3689847
GGGATCA203.30623E-452.3689847
AAGTGGT150.006991670552.36898410
TGGGAAT150.006991670552.36898410
GGGGATA150.006991670552.3689846
AGTTCTG150.006991670552.36898410
GTGTGCT203.30623E-452.36898410
GGTGCTT353.6019628E-852.3689848
GGAGCAA203.30623E-452.3689848
AAGCATG353.6019628E-852.36898410
GACTAAT203.30623E-452.3689849
ACCCAGT150.0071623652.05087750
Produced by FastQC (version 0.11.5)