FastQCFastQC Report
Tue 31 May 2016
SRR212524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62326
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13622.1852838301832302No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA10971.7601001187305458No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC10731.7215929146744537No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8591.3782370118409653No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7561.2129769277669031No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7431.1921188589031864No Hit
AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6050.9707024355806565No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5480.8792478259474377No Hit
AAGCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4050.6498090684465552No Hit
AAGCGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3750.60167506337644No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3640.5840259281840644No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3480.5583544588133363No Hit
AAGCGGGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3270.5246606552642556No Hit
AAGCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3180.5102204537432211No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2790.44764624715207135No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2570.4123479767673202No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2110.3385425023264769No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1910.3064531656130668No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1860.2984308314347142No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1740.27917722940666817No Hit
AAGCGGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1710.27436382889965666No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1610.25831916054295156No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1600.25671469370728106No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.22783429066521194No Hit
AAGCGGGGGGAATTTATTTTGTTATACTCTAGCCTAGTAATAATTGGCAATTGGTT1420.22783429066521194No Hit
AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1400.22462535699387093No Hit
AAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1310.21018515547283637No Hit
AAGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.20858068863716586No Hit
AAGCGGGGGCCTGAAGAGGTCACTGAAGAGTAAGGTCCCTGGAGGAGTCAGTAGGG1190.1909315534447903No Hit
AAGCGGGGGCATTTGTGTAGTTTACCACTAAAGGAGTTTGGATAAGAATATGTGCA1160.18611815293777878No Hit
AAGCGGGGGAGTTGTGTAATATTTTGTCCTTTGTAACTATGTAAACATTGTCTATT1150.18451368610210825No Hit
AAGCGGGGGCGGCAGTCTCTGGTCTCACTATTCTTTCTGAGTGCTACGTGGGACTG1150.18451368610210825No Hit
AAGCGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1120.17970028559509674No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1110.17809581875942623No Hit
AAGCGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1110.17809581875942623No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1090.17488688508808523No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC1090.17488688508808523No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1070.17167795141674422No Hit
AAGCGGGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCA1060.17007348458107371No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1060.17007348458107371No Hit
AAGCGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1060.17007348458107371No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA1050.1684690177454032No Hit
AAGCGGGGGATCTTCATCTGTCTAAGGATATCTTAATTAGTAGACCAACAAAGATC1050.1684690177454032No Hit
AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1050.1684690177454032No Hit
AAGCGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1000.16044668356705066No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGA980.15723774989570966No Hit
AAGCGGGGGAGTTCTGTGAATTGAGCTACACAGCGTGATGAGATGCGCGTGCCTGG930.1492154157173571No Hit
AAGCGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG920.1476109488816866No Hit
AAGCGGGGGGGGAATGCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA920.1476109488816866No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC920.1476109488816866No Hit
AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC910.1460064820460161No Hit
AAGCGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.1460064820460161No Hit
AAGCGGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC900.1444020152103456No Hit
AAGCGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT890.1427975483746751No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCTCTTCC880.1411930815390046No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTC840.13477521419632257No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC840.13477521419632257No Hit
AAGCGGGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG830.13317074736065207No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTT830.13317074736065207No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT830.13317074736065207No Hit
AAGCGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC820.13156628052498154No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTACTCTTCC810.12996181368931103No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC790.12675288001797003No Hit
AAGCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC760.12193947951095852No Hit
AAGCGGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC750.12033501267528801No Hit
AAGCGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC730.117126079003947No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGTTCTTCC720.11552161216827649No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCG710.11391714533260597No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCA710.11391714533260597No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTC700.11231267849693546No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG700.11231267849693546No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC680.10910374482559446No Hit
AAGCGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG680.10910374482559446No Hit
AAGCGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA680.10910374482559446No Hit
AAGCGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC670.10749927798992395No Hit
AAGCGGGGGAAGGCAACAATAGAATTATTAAGATATTAAACTCTTCTGATATGTCA670.10749927798992395No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC660.10589481115425345No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG650.10429034431858293No Hit
AAGCGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA640.10268587748291243No Hit
AAGCGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10268587748291243No Hit
AAGCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC640.10268587748291243No Hit
AAGCGGGGGGTCACCAGCGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCG630.10108141064724192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTA251.9896832E-550.31255348
TGTCTAT251.9896832E-550.31255349
GTCTATT251.9896832E-550.31255350
TTGCAAT150.00818930450.3125549
GAAACAC204.0272257E-450.3125550
CCAGAAA204.0272257E-450.3125548
TCAGCTC150.00818930450.3125550
GCAGTGA150.00818930450.3125550
GCAAAGT150.00818930450.3125549
TGCTCTG150.00818930450.3125549
TCTCGCA204.0272257E-450.3125548
ATTGATA150.00818930450.3125548
TCGCAAA204.0272257E-450.3125550
TTGGTTT150.00818930450.3125549
CAGAAAC204.0272257E-450.3125549
ACAGGAC150.00818930450.3125550
TTCAGCT150.00818930450.3125549
CTCGCAA204.0272257E-450.3125549
AATTGGT150.00818930450.3125549
GTTGCAA150.00818930450.3125548
Produced by FastQC (version 0.11.5)