FastQCFastQC Report
Tue 31 May 2016
SRR212523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60662
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11891.960040882265669No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11571.9072895717252976No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA10121.6682601958392405No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC9981.645181497477828No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8521.404503643137384No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7381.2165770993373115No Hit
AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6271.0335959909003989No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6000.9890870726319606No Hit
AAGCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4840.797863571923115No Hit
AAGCGGGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3740.6165309419405889No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3650.6016946358511095No Hit
AAGCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3470.5720220236721506No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3300.5439978899475784No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3140.5176222346773928No Hit
AAGCGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3130.5159737562230061No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2450.40387722132471726No Hit
AAGCGGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1990.32804721242293367No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1970.3247502555141604No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1950.32145329860538724No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1870.30826547097029444No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1610.2654050311562428No Hit
AAGCGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1570.2588111173386964No Hit
AAGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.25551416042992314No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1550.25551416042992314No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1510.2489202466123768No Hit
AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1430.23573241897728398No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.22749002670535098No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1350.22254459134219115No Hit
AAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1290.21265372061587154No Hit
AAGCGGGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1220.20111437143516533No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1140.18792654380007254No Hit
AAGCGGGGGCATTTGTGTAGTTTACCACTAAAGGAGTTTGGATAAGAATATGTGCA1140.18792654380007254No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1140.18792654380007254No Hit
AAGCGGGGGGAATTTATTTTGTTATACTCTAGCCTAGTAATAATTGGCAATTGGTT1130.18627806534568592No Hit
AAGCGGGGGATCTTCATCTGTCTAAGGATATCTTAATTAGTAGACCAACAAAGATC1130.18627806534568592No Hit
AAGCGGGGGCGGCAGTCTCTGGTCTCACTATTCTTTCTGAGTGCTACGTGGGACTG1120.18462958689129935No Hit
AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1100.18133262998252614No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1070.17638719461936633No Hit
AAGCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1070.17638719461936633No Hit
AAGCGGGGGCCTGAAGAGGTCACTGAAGAGTAAGGTCCCTGGAGGAGTCAGTAGGG1020.16814480234743331No Hit
AAGCGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1020.16814480234743331No Hit
AAGCGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1010.16649632389304672No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCTCTTCC980.16155088852988692No Hit
AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGA980.16155088852988692No Hit
AAGCGGGGGAGTTCTGTGAATTGAGCTACACAGCGTGATGAGATGCGCGTGCCTGG970.15990241007550032No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA960.1582539316211137No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC950.1566054531667271No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCAAGCGAC940.1549569747123405No Hit
AAGCGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC930.15330849625795392No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC900.1483630608947941No Hit
AAGCGGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC900.1483630608947941No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC900.1483630608947941No Hit
AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC880.1450661039860209No Hit
AAGCGGGGGGGGAATGCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA870.1434176255316343No Hit
AAGCGGGGGAGTTGTGTAATATTTTGTCCTTTGTAACTATGTAAACATTGTCTATT870.1434176255316343No Hit
AAGCGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.1401206686228611No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT850.1401206686228611No Hit
AAGCGGGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCA840.13847219016847448No Hit
AAGCGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT830.13682371171408791No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC830.13682371171408791No Hit
AAGCGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC830.13682371171408791No Hit
AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG820.1351752332597013No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTACTCTTCC800.13187827635092808No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTC790.13022979789654152No Hit
AAGCGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC730.12033892717022189No Hit
AAGCGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG720.11869044871583528No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTT720.11869044871583528No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC700.11539349180706207No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA690.11374501335267549No Hit
AAGCGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG690.11374501335267549No Hit
AAGCGGGGGGCCCCCGCTGCCGCGGAATTAGGCATCGTCTGCCAGGATTGCGAGAC680.11209653489828889No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA680.11209653489828889No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG670.11044805644390228No Hit
AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC670.11044805644390228No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG660.10879957798951567No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCG650.10715109953512907No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC650.10715109953512907No Hit
AAGCGGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC650.10715109953512907No Hit
AAGCGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG640.10550262108074249No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGTTCTTCC640.10550262108074249No Hit
AAGCGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10550262108074249No Hit
AAGCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC630.10385414262635588No Hit
AAGCGGGGGGAGCAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAGA620.10220566417196927No Hit
AAGCGGGGGAAGGCAACAATAGAATTATTAAGATATTAAACTCTTCTGATATGTCA620.10220566417196927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA203.9720983E-450.45067250
AACAAGC251.9569254E-550.4506750
CCTATGT150.00809980150.4506749
GCGACTG309.69274E-750.4506750
GATACAA150.00809980150.4506750
TTCAACT150.00809980150.4506750
ATTGGTT150.00809980150.4506750
TGATACA150.00809980150.4506749
GAACAAG251.9569254E-550.4506749
CAGGCTA150.00809980150.4506749
CTATGTC150.00809980150.4506750
TGGGACT150.00809980150.4506749
CAAGAAC251.9666444E-550.4085246
GTCATCA150.00812663650.4085246
GGCAATT150.00812663650.4085246
TATGTTC150.00812663650.4085246
CCTGATA150.00812663650.4085247
TGTTCAA150.00812663650.4085248
CTGATAC150.00812663650.4085248
AGAACAA251.9666444E-550.4085248
Produced by FastQC (version 0.11.5)