##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212523_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60662 Sequences flagged as poor quality 0 Sequence length 56 %GC 58 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.12203685997824 39.0 38.0 40.0 35.0 40.0 2 38.071659358412184 39.0 38.0 40.0 35.0 40.0 3 37.31695295242491 39.0 37.0 39.0 33.0 40.0 4 37.183887771586825 39.0 37.0 39.0 33.0 40.0 5 37.665688569450396 39.0 38.0 40.0 35.0 40.0 6 37.58690778411526 39.0 38.0 40.0 33.0 40.0 7 38.1293890738848 39.0 38.0 40.0 35.0 40.0 8 37.33808644621015 39.0 35.0 40.0 34.0 40.0 9 37.29867132636576 39.0 35.0 40.0 34.0 40.0 10 36.391085028518674 39.0 35.0 39.0 31.0 40.0 11 36.46030463881837 39.0 35.0 39.0 31.0 40.0 12 36.6900036266526 39.0 35.0 40.0 32.0 40.0 13 36.59099601068214 39.0 35.0 39.0 31.0 40.0 14 36.84683986680294 39.0 36.0 40.0 32.0 40.0 15 36.91361972899014 39.0 36.0 40.0 33.0 40.0 16 36.765883089908016 38.0 36.0 40.0 32.0 40.0 17 36.201592430186935 38.0 35.0 39.0 30.0 40.0 18 36.06888991460882 38.0 35.0 39.0 30.0 40.0 19 35.8447957535195 38.0 35.0 39.0 29.0 40.0 20 35.78022485246118 38.0 35.0 39.0 29.0 40.0 21 35.25434374072731 38.0 35.0 39.0 29.0 40.0 22 35.09953512907586 38.0 34.0 39.0 28.0 40.0 23 34.99830206719198 38.0 34.0 39.0 28.0 40.0 24 34.76223995252382 38.0 33.0 39.0 27.0 40.0 25 34.60797533876232 38.0 33.0 39.0 27.0 40.0 26 34.02756255975734 37.0 33.0 39.0 26.0 40.0 27 33.689344235270845 36.0 33.0 39.0 25.0 40.0 28 33.20980185288978 36.0 32.0 39.0 23.0 40.0 29 33.05222379743497 36.0 32.0 38.0 23.0 40.0 30 32.61836075302496 36.0 31.0 38.0 21.0 40.0 31 31.710659061686062 35.0 30.0 38.0 17.0 40.0 32 31.81800797863572 35.0 30.0 38.0 17.0 40.0 33 31.438313936236852 35.0 30.0 38.0 17.0 39.0 34 31.020605980679832 35.0 29.0 38.0 15.0 40.0 35 30.163347730045167 34.0 28.0 38.0 7.0 39.0 36 30.493109360060664 35.0 30.0 38.0 2.0 40.0 37 29.765058850680823 34.0 29.0 38.0 2.0 39.0 38 29.315683624015033 34.0 28.0 38.0 2.0 39.0 39 29.187399030694667 34.0 27.0 38.0 2.0 39.0 40 29.001879265438 34.0 27.0 38.0 2.0 39.0 41 28.19316870528502 34.0 25.0 38.0 2.0 39.0 42 27.788566153440375 33.0 23.0 38.0 2.0 39.0 43 27.731776070686756 33.0 24.0 38.0 2.0 39.0 44 27.54678381853549 33.0 23.0 38.0 2.0 39.0 45 26.77874122185223 33.0 21.0 37.0 2.0 39.0 46 25.80236391810359 32.0 15.0 36.0 2.0 39.0 47 25.47373973822162 32.0 13.0 36.0 2.0 39.0 48 25.47105271834097 32.0 12.0 36.0 2.0 39.0 49 25.036579736902837 32.0 2.0 36.0 2.0 39.0 50 24.573456199927467 31.0 2.0 36.0 2.0 39.0 51 23.75043684679041 31.0 2.0 36.0 2.0 39.0 52 23.483300913257064 31.0 2.0 35.0 2.0 39.0 53 23.064274174936532 31.0 2.0 35.0 2.0 39.0 54 22.901569351488575 31.0 2.0 35.0 2.0 39.0 55 22.448254261316805 31.0 2.0 35.0 2.0 39.0 56 13.034914773663909 17.0 2.0 22.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.6801483211806456 1 2 -2.3430107048298865 1 3 -2.000709240377624 1 4 -1.7137490184151716 1 5 -1.82319493685565 1 6 -1.7782926356892688 1 7 -2.208086734019453 1 8 -2.3944541026729596 1 9 -2.1795682033171175 1 10 -2.0943335149677154 1 11 -2.267781290426221 1 12 -2.5263298176159807 1 13 -1.725372971554897 1 14 -1.3446266428035898 1 15 -2.4889983712348496 1 16 -2.002507014473686 1 17 -1.1769974128448482 1 18 -2.1835917694758606 1 19 -1.131060111515957 1 20 -1.7512286572972044 1 21 -0.12148278376316313 1 22 -0.4843047739176356 1 23 -0.8872115035056396 1 24 -0.7705305985776718 1 25 -0.20555201019866587 1 26 -0.4687574538932182 1 27 -0.8215941103581201 1 28 0.1759489160962957 1 29 -0.6717995430912396 1 30 -0.5177972958875792 1 31 -0.7809206837701943 1 32 0.11734114812203345 1 33 -1.2088141068855265 1 34 -0.14241728322512515 1 35 0.599925621896034 1 36 -0.6416180444974309 1 37 0.26100815853633463 1 38 0.5028841681387668 1 39 0.006043397263088224 1 40 0.18713423735757218 1 41 0.06006517078550644 1 42 1.2237866741567132 1 43 0.49426060329013666 1 44 0.2921878120768753 1 45 1.403385898060833 1 46 1.0468574246228783 1 47 1.760593354524925 1 48 1.395948600046399 1 49 2.412255191492463 1 50 1.7153266263131393 1 51 1.093232870642062 1 52 1.6544525148678666 1 53 2.2954640423571924 1 54 1.9059645130773504 1 55 2.598357979091908 1 56 1.352344858450504 2 1 -1.0530054640377884 2 2 -1.3037249905441683 2 3 -1.432852097520481 2 4 -2.3487490184151696 2 5 -1.4446235082842165 2 6 -1.166149778546412 2 7 -0.5030867340194547 2 8 -1.265168388387245 2 9 -1.1109967747456864 2 10 -1.9443335149677168 2 11 -1.852781290426222 2 12 -1.4177583890445504 2 13 -2.5668015429834696 2 14 -1.106769499946445 2 15 -1.241855514091995 2 16 -0.36107844304511616 2 17 -0.483425984273417 2 18 -0.19359176947585865 2 19 0.34608274562689445 2 20 -0.4176572287257727 2 21 -0.9393399266203062 2 22 -0.0021619167747815027 2 23 0.4506456393515066 2 24 -0.40910202714910326 2 25 -0.06412343877009619 2 26 -1.3387574538932157 2 27 -0.2573083960724034 2 28 -1.0004796553322706 2 29 -0.8167995430912391 2 30 -0.8549401530304372 2 31 -0.5244921123416226 2 32 -0.10837313759225253 2 33 -2.070956964028383 2 34 -1.9452744260822676 2 35 -1.5850743781039647 2 36 -0.8823323302117139 2 37 -1.2561346986065232 2 38 -1.3199729747183753 2 39 0.09032911154880452 2 40 -1.8414371912138563 2 41 -0.6327919720716366 2 42 0.6923581027281394 2 43 -0.31359653956700484 2 44 -0.11352647363741042 2 45 -0.21089981622488096 2 46 -0.29028543251997974 2 47 1.5548790688106386 2 48 0.2609486000463974 2 49 0.5301123343496066 2 50 -0.906816230829719 2 51 -0.651767129357939 2 52 -0.6562617708464167 2 53 -1.8252502433570932 2 54 -0.7897497726369345 2 55 0.6169294076633349 2 56 1.506630572736217 3 1 -0.2994205583774132 3 2 -0.30561178299700487 3 3 -0.27058794657708063 3 4 0.11879815139614891 3 5 -0.7578310554540337 3 6 -0.3574705332633954 3 7 0.18106420937677115 3 8 -0.1542249921608274 3 9 -0.47741186908530864 3 10 -1.2499938923262093 3 11 -1.3361775168413175 3 12 -1.2015319739502104 3 13 -1.509065693926864 3 14 -2.2041279905124824 3 15 -0.9573272122052003 3 16 -0.37919165059228277 3 17 -1.6139920220092634 3 18 -1.1411389392871811 3 19 -0.056936122297628344 3 20 -1.452374209857851 3 21 -0.5733021907712512 3 22 -0.13423738847289712 3 23 -0.8504864361201925 3 24 -0.7132529705453265 3 25 -0.591670608581417 3 26 -0.5678140576668014 3 27 -0.6591951885252385 3 28 -0.5487815421247291 3 29 -0.4005731279968998 3 30 0.3986447526299415 3 31 -1.3203411689453937 3 32 -0.9891278545733826 3 33 -1.134353190443477 3 34 -0.8418781996671747 3 35 -0.15714984980207802 3 36 0.7165355943165856 3 37 0.026506810827438443 3 38 0.7656874026401148 3 39 0.1590083568318228 3 40 -0.3074749270629127 3 41 1.7709816128340243 3 42 2.105188291407387 3 43 0.6886676113763919 3 44 1.79892635655127 3 45 1.7457039573600248 3 46 0.8217900391781328 3 47 1.0503507669238488 3 48 1.6247221849520592 3 49 1.2225651645382847 3 50 0.5822403729438648 3 51 0.9399309838496102 3 52 0.8003420027384891 3 53 0.2102214547561161 3 54 1.0494955103819343 3 55 1.2674954453991845 3 56 1.3307815161324434 4 1 0.07514268411035374 4 2 0.23701575019656929 4 3 -0.3069261715945544 4 4 0.189028759362607 4 5 0.5775987139380021 4 6 -0.24985348225011705 4 7 0.23172808079536367 4 8 -0.06442764764650377 4 9 -0.6028486265975417 4 10 -0.5850742557084558 4 11 -0.6520405496854806 4 12 0.23557494428878556 4 13 0.0828280866461597 4 14 -0.8082509814279248 4 15 -0.011485143721621682 4 16 0.022625260658585944 4 17 0.5802777194302848 4 18 0.39974156385747506 4 19 -0.273917254373103 4 20 -0.3458053768739191 4 21 -0.13933992662030903 4 22 1.3311714165585542 4 23 0.5565715652774301 4 24 -0.5972501752972477 4 25 0.6899506353039797 4 26 1.6516129164771556 4 27 1.568617529853519 4 28 2.312853678001062 4 29 1.3143115680198747 4 30 1.6065413284510441 4 31 1.4895819617324548 4 32 2.0908861216670083 4 33 1.9201541470827301 4 34 2.3073181665103277 4 35 3.2341848811552936 4 36 1.6050750771956928 4 37 1.4779393754675496 4 38 2.5266936919482923 4 39 2.5999587411784333 4 40 2.795599845823183 4 41 2.4635043242246617 4 42 2.8930988434688807 4 43 3.2264034604329943 4 44 3.710177230066293 4 45 4.244655739330675 4 46 5.560084937850391 4 47 6.715619809551381 4 48 4.113541192638991 4 49 5.707890112127384 4 50 4.788739324725839 4 51 3.468232870642062 4 52 2.762256747672101 4 53 2.9125275344206862 4 54 2.8332131903260276 4 55 4.073225703959633 4 56 0.36292686903251337 5 1 0.12699453596221133 5 2 -0.2946340814532604 5 3 -0.16012482479320767 5 4 -0.7614762911424435 5 5 -0.5173507810114941 5 6 0.30839567599903717 5 7 0.37145872052600026 5 8 0.149377066158209 5 9 0.36718504343613034 5 10 0.4920301213959206 5 11 -0.6291449267898557 5 12 0.12951433822817648 5 13 -0.01952881571074272 5 14 0.027775954599007946 5 15 0.5181444859080031 5 16 -0.17198753395420852 5 17 -0.5234259842734161 5 18 -0.1335917694758635 5 19 -0.7920990725549188 5 20 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6 6 0.022477672433979023 6 7 0.56397208950996 6 8 0.6164002390637364 6 9 0.690179695842545 6 10 0.7733135438558136 6 11 0.058199101730643577 6 12 -0.06834662433866612 6 13 0.9630023785851591 6 14 0.1732305000535561 6 15 0.6867719368883982 6 16 0.10323528244507685 6 17 0.30794656474618876 6 18 0.6154278383672747 6 19 0.6182396083719937 6 20 0.4592055163722648 6 21 0.2920326223992973 6 22 0.48803416165659286 6 23 0.01299858052797731 6 24 -0.03733732126674738 6 25 0.16489616907303883 6 26 0.08516411473423346 6 27 -0.43573976862142416 6 28 -0.49106789062638967 6 29 0.8698671235754247 6 30 0.42898141559701486 6 31 1.2782529856975913 6 32 1.645352352603826 6 33 1.8482587222461255 6 34 1.2931569464667518 6 35 1.7984550336607406 6 36 1.7957068854745621 6 37 2.031316281785635 6 38 2.1737525154777018 6 39 2.891897738999784 6 40 2.2389549656488903 6 41 3.9123060671440513 6 42 3.69157378900265 6 43 2.1577760094526006 6 44 3.4574539185194517 6 45 2.7871393994613918 6 46 2.003440057676098 6 47 2.778800637438092 6 48 2.118987815732673 6 49 3.077171157879018 6 50 3.2233798476016524 6 51 3.6643113020146103 6 52 3.077071562486914 6 53 3.879847795858595 6 54 4.5075051293238495 6 55 4.973007839035883 6 56 1.576434494304845 7 1 -0.17300546403779293 7 2 -0.3237249905441715 7 3 -0.8528520975204756 7 4 -0.708749018415169 7 5 -0.24462350828422075 7 6 -0.1461497785464161 7 7 -0.08308673401945299 7 8 -0.1851683883872468 7 9 -0.05099677474568409 7 10 -0.9043335149677176 7 11 -0.012781290426218561 7 12 -0.6377583890445493 7 13 0.2131984570165315 7 14 -0.04676949994644275 7 15 -0.7018555140919958 7 16 -1.561078443045119 7 17 -0.8634259842734195 7 18 -0.9135917694758575 7 19 -0.15391725437310555 7 20 -1.1976572287257738 7 21 -1.0193399266203116 7 22 -1.4221619167747832 7 23 -0.32935436064849455 7 24 -1.0091020271491047 7 25 -2.2241234387701 7 26 -0.49875745389321935 7 27 0.5426916039275937 7 28 0.019520344667725453 7 29 -0.5767995430912407 7 30 0.06505984696956091 7 31 -0.7444921123416215 7 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0.33815232618565005 8 17 0.934266323418889 8 18 1.0856389997549059 8 19 0.6683904379345904 8 20 0.43695815588961295 8 21 0.45681391953353767 8 22 -0.3290849936978617 8 23 -0.5808928221869536 8 24 0.3585902805432042 8 25 0.48126117661451673 8 26 0.9735502384144752 8 27 1.1619223731583617 8 28 1.0179818831292664 8 29 1.222431226139527 8 30 1.7019829238926434 8 31 1.1655078876583786 8 32 1.485473016253902 8 33 1.385966112894696 8 34 1.695494804686966 8 35 1.186464083434494 8 36 1.9861292082498245 8 37 2.576942224470397 8 38 2.0138731791277777 8 39 1.9318675730872634 8 40 1.3931781934015284 8 41 1.378746489466824 8 42 0.4023581027281402 8 43 0.822557306586841 8 44 1.5833966032856672 8 45 1.5352540299289643 8 46 0.9012530290184806 8 47 0.2056482995798703 8 48 0.02094860004639898 8 49 1.8531892574265285 8 50 1.5024145384010517 8 51 2.6220790244882153 8 52 1.8783536137689651 8 53 1.6155189874121376 8 54 2.3510194581322956 8 55 2.392314023047952 8 56 1.5858613419669876 9 1 0.1412802502479238 9 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49.0 3 45.0 4 60.0 5 32.0 6 23.0 7 50.0 8 49.0 9 94.0 10 194.0 11 185.0 12 311.0 13 407.0 14 468.0 15 417.0 16 474.0 17 553.0 18 619.0 19 673.0 20 797.0 21 854.0 22 1008.0 23 1376.0 24 1609.0 25 1529.0 26 1427.0 27 1509.0 28 1605.0 29 1861.0 30 2110.0 31 2429.0 32 2888.0 33 3680.0 34 4788.0 35 5920.0 36 6923.0 37 6693.0 38 4960.0 39 1993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 67.84807622564372 13.730177046586 4.371764861033267 14.049981866737001 11 42.728561537700706 13.730177046586 13.651050080775445 29.890211334937856 12 40.16352906267515 8.164913784576836 28.650555537239132 23.021001615508887 13 20.680162210279914 14.989614585737366 34.33121229105536 29.99901091292737 14 16.53259041904322 17.39309617223303 45.46668425043684 20.6076291582869 15 25.037090765223695 10.972272592397218 45.84748277340015 18.14315386897893 16 30.60729946259602 11.265701757278032 34.2768125020606 23.850186278065348 17 26.34927961491543 12.238304045366128 24.277142197751473 37.13527414196696 18 32.20797204180542 14.706076291582871 24.141966964491772 28.943984702119945 19 31.431538689789324 21.181299660413437 28.962117965118196 18.42504368467904 20 34.31637598496587 17.749167518380535 30.00230786983614 17.932148626817447 21 29.88691437802908 19.572384688932114 27.64168672315453 22.899014209884278 22 34.903234314727506 16.570505423494115 30.378160957436286 18.14809930434209 23 42.457262491551404 16.363066879873394 24.16709253062099 17.012578097954204 24 35.67036481429584 19.29081287812598 24.58086744366232 20.457954863915862 25 27.519168933959932 23.80080798087229 21.7116002968093 26.96842278835848 26 26.68085597848504 17.84883433153492 26.174330544968573 29.29597914501147 27 22.689616476335072 15.911941382269458 23.054327018285033 38.344115123110434 28 27.221259387637204 29.25971775191879 18.714203185606998 24.804819674837006 29 34.32623879266219 18.13813714644255 24.58431715734029 22.951306903554975 30 27.280087238120416 18.338179895578612 28.94554226422576 25.43619060207521 31 36.919771957365946 16.138147566718995 25.09790960918781 21.844170866727257 32 45.93388429752066 12.31900826446281 22.474380165289258 19.272727272727273 33 44.518113126436404 11.980622013524908 24.74330781567765 18.75795704436104 34 30.367920628358824 16.99049193881769 35.61967755270773 17.02190988011575 35 24.52730310499413 27.238589932341895 33.87206166978214 14.36204529288184 36 32.58635242713626 32.263616954370164 20.835471111037553 14.314559507456018 37 35.3935001323802 21.953600741329097 22.641977760127084 20.010921366163622 38 29.704445732262602 26.43596324199023 24.973921682258464 18.8856693434887 39 35.147580084142184 20.785437439957597 23.655878358233675 20.411104117666547 40 31.11195798054778 20.950077046708532 21.491889384123407 26.446075588620282 41 31.02099663589812 21.057952040833236 25.878726612863133 22.042324710405513 42 27.410637310166457 18.80927117182837 26.225213873599046 27.55487764440613 43 25.850148392552185 16.581831445956922 22.910483643658914 34.65753651783198 44 25.52803474916277 14.188136211412845 26.421632016976695 33.862197022447695 45 32.14054753179564 19.085678279470045 23.159832191951182 25.613941996783122 46 27.129944439837466 21.24720126046936 23.593996185421677 28.0288581142715 47 26.21299615027214 18.223151466879063 26.666002920483205 28.897849462365592 48 28.58992662438992 15.711012981838707 27.590557455426808 28.108502938344564 49 27.06805587815642 17.999469091150413 27.65537379301191 27.277101237681258 50 22.129065462454136 17.883884249497783 29.347699765909052 30.639350522139026 51 22.648366967604886 15.553637812002124 32.91290493892725 28.885090281465747 52 20.66989936563818 19.088976717924876 34.815171543392346 25.425952373044602 53 15.868059592419739 18.708166553173115 39.41769502898238 26.006078825424773 54 19.761861268412577 18.47152797382799 33.54036235614527 28.226248401614164 55 20.608124948076764 16.58552795547063 29.635291185511342 33.17105591094126 56 21.038555457731857 21.734580308643 21.95883652552368 35.268027708101464 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 10.0 13 30.5 14 41.0 15 48.0 16 55.0 17 72.0 18 89.0 19 259.0 20 429.0 21 409.5 22 381.5 23 373.0 24 399.0 25 425.0 26 511.0 27 597.0 28 623.5 29 631.0 30 612.0 31 657.0 32 702.0 33 775.0 34 848.0 35 751.5 36 655.0 37 745.5 38 699.5 39 563.0 40 574.5 41 586.0 42 589.5 43 593.0 44 544.5 45 496.0 46 507.0 47 1075.0 48 1632.0 49 1305.5 50 979.0 51 947.0 52 915.0 53 1104.0 54 1881.5 55 2470.0 56 2628.5 57 2787.0 58 3151.0 59 3515.0 60 4307.0 61 5099.0 62 5654.5 63 6597.0 64 6984.0 65 7055.5 66 7127.0 67 5888.0 68 4649.0 69 3639.5 70 2630.0 71 2134.0 72 1371.0 73 1104.0 74 1007.0 75 910.0 76 745.0 77 580.0 78 437.5 79 226.0 80 157.0 81 123.5 82 90.0 83 75.0 84 60.0 85 43.0 86 26.0 87 20.0 88 11.5 89 9.0 90 7.5 91 6.0 92 4.5 93 3.0 94 1.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0016484784543866014 24 0.0016484784543866014 25 0.02802413372457222 26 0.08736935808248986 27 0.10879957798951567 28 0.1269328409877683 29 0.1631993669842735 30 0.22749002670535098 31 0.24232633279483037 32 0.2670535096106294 33 0.29837460024397483 34 0.30826547097029444 35 0.34782895387557283 36 0.3972833075071709 37 0.37915004450891826 38 0.44014374732122247 39 0.47476179486334114 40 0.5093798424054597 41 0.5258646269493258 42 0.570373545217764 43 0.5753189805809239 44 0.5670765883089908 45 0.5852098513072435 46 0.6049915927598826 47 0.6560944248458673 48 0.6989548646599189 49 0.6396096403020013 50 0.7071972569318519 51 0.6692822524809601 52 0.7319244337476509 53 0.7467607398371303 54 0.7335729122020376 55 0.7863242227424088 56 0.7632455243809964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 60662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.23042431835416 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37793613090525 22.603936566549077 2 10.261282660332542 6.409284230655106 3 4.8403272631301135 4.5349642280175395 4 2.7025600422275007 3.3760838745837596 5 1.9107944048561625 2.983746002439748 6 1.2826603325415677 2.4034815864956647 7 0.9765109527579837 2.1347795984306486 8 0.6967537608867775 1.7407932478322508 9 0.5911850092372658 1.661666282021694 >10 3.7846397466349964 22.22313804358577 >50 0.35365531802586436 7.709933731166134 >100 0.17946687780416995 10.39365665490752 >500 0.026392187912377938 6.2889453034848835 >1k 0.015835312747426764 5.5355906498302065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1189 1.960040882265669 No Hit AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1157 1.9072895717252976 No Hit AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1012 1.6682601958392405 No Hit AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 998 1.645181497477828 No Hit AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 852 1.404503643137384 No Hit AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 738 1.2165770993373115 No Hit AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 627 1.0335959909003989 No Hit AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 600 0.9890870726319606 No Hit AAGCGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 484 0.797863571923115 No Hit AAGCGGGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 374 0.6165309419405889 No Hit AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 365 0.6016946358511095 No Hit AAGCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 347 0.5720220236721506 No Hit AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 330 0.5439978899475784 No Hit AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 314 0.5176222346773928 No Hit AAGCGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 313 0.5159737562230061 No Hit AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 245 0.40387722132471726 No Hit AAGCGGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 199 0.32804721242293367 No Hit AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 197 0.3247502555141604 No Hit AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 195 0.32145329860538724 No Hit AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 187 0.30826547097029444 No Hit AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 161 0.2654050311562428 No Hit AAGCGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 157 0.2588111173386964 No Hit AAGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.25551416042992314 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 155 0.25551416042992314 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 151 0.2489202466123768 No Hit AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 143 0.23573241897728398 No Hit AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.22749002670535098 No Hit AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 135 0.22254459134219115 No Hit AAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 129 0.21265372061587154 No Hit AAGCGGGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 122 0.20111437143516533 No Hit AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 114 0.18792654380007254 No Hit AAGCGGGGGCATTTGTGTAGTTTACCACTAAAGGAGTTTGGATAAGAATATGTGCA 114 0.18792654380007254 No Hit AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 114 0.18792654380007254 No Hit AAGCGGGGGGAATTTATTTTGTTATACTCTAGCCTAGTAATAATTGGCAATTGGTT 113 0.18627806534568592 No Hit AAGCGGGGGATCTTCATCTGTCTAAGGATATCTTAATTAGTAGACCAACAAAGATC 113 0.18627806534568592 No Hit AAGCGGGGGCGGCAGTCTCTGGTCTCACTATTCTTTCTGAGTGCTACGTGGGACTG 112 0.18462958689129935 No Hit AAGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 110 0.18133262998252614 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 107 0.17638719461936633 No Hit AAGCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 107 0.17638719461936633 No Hit AAGCGGGGGCCTGAAGAGGTCACTGAAGAGTAAGGTCCCTGGAGGAGTCAGTAGGG 102 0.16814480234743331 No Hit AAGCGGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 102 0.16814480234743331 No Hit AAGCGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG 101 0.16649632389304672 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGCTCTTCC 98 0.16155088852988692 No Hit AAGCGGGGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGA 98 0.16155088852988692 No Hit AAGCGGGGGAGTTCTGTGAATTGAGCTACACAGCGTGATGAGATGCGCGTGCCTGG 97 0.15990241007550032 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA 96 0.1582539316211137 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC 95 0.1566054531667271 No Hit AAGCGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCAAGCGAC 94 0.1549569747123405 No Hit AAGCGGGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 93 0.15330849625795392 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 90 0.1483630608947941 No Hit AAGCGGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC 90 0.1483630608947941 No Hit AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 90 0.1483630608947941 No Hit AAGCGGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC 88 0.1450661039860209 No Hit AAGCGGGGGGGGAATGCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 87 0.1434176255316343 No Hit AAGCGGGGGAGTTGTGTAATATTTTGTCCTTTGTAACTATGTAAACATTGTCTATT 87 0.1434176255316343 No Hit AAGCGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.1401206686228611 No Hit AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 85 0.1401206686228611 No Hit AAGCGGGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCA 84 0.13847219016847448 No Hit AAGCGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT 83 0.13682371171408791 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC 83 0.13682371171408791 No Hit AAGCGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 83 0.13682371171408791 No Hit AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 82 0.1351752332597013 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTACTCTTCC 80 0.13187827635092808 No Hit AAGCGGGGGGGGCTGGAGAGATGGCTCAACAGTTAAGAGCACTGACTGCCCTCTTC 79 0.13022979789654152 No Hit AAGCGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC 73 0.12033892717022189 No Hit AAGCGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 72 0.11869044871583528 No Hit AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTT 72 0.11869044871583528 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC 70 0.11539349180706207 No Hit AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA 69 0.11374501335267549 No Hit AAGCGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 69 0.11374501335267549 No Hit AAGCGGGGGGCCCCCGCTGCCGCGGAATTAGGCATCGTCTGCCAGGATTGCGAGAC 68 0.11209653489828889 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA 68 0.11209653489828889 No Hit AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG 67 0.11044805644390228 No Hit AAGCGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 67 0.11044805644390228 No Hit AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 66 0.10879957798951567 No Hit AAGCGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCG 65 0.10715109953512907 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC 65 0.10715109953512907 No Hit AAGCGGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 65 0.10715109953512907 No Hit AAGCGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 64 0.10550262108074249 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTCGACTGTTCTTCC 64 0.10550262108074249 No Hit AAGCGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 0.10550262108074249 No Hit AAGCGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC 63 0.10385414262635588 No Hit AAGCGGGGGGAGCAGTTCCGGTGAAGCTCCGGGAGTTTGTTTACGTTCCGAGAAGA 62 0.10220566417196927 No Hit AAGCGGGGGAAGGCAACAATAGAATTATTAAGATATTAAACTCTTCTGATATGTCA 62 0.10220566417196927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0016484784543866011 0.0 0.0 0.0 0.0 11 0.004945435363159803 0.0 0.0 0.0 0.0 12 0.004945435363159803 0.0 0.0 0.0 0.0 13 0.004945435363159803 0.0 0.0 0.0 0.0 14 0.008242392271933005 0.0 0.0 0.0 0.0 15 0.008242392271933005 0.0 0.0 0.0 0.0 16 0.008242392271933005 0.0 0.0 0.0 0.0 17 0.008242392271933005 0.0 0.0 0.0 0.0 18 0.008242392271933005 0.0 0.0 0.0 0.0 19 0.008242392271933005 0.0 0.0 0.0 0.0016484784543866011 20 0.008242392271933005 0.0 0.0 0.0 0.0016484784543866011 21 0.008242392271933005 0.0 0.0 0.0 0.0016484784543866011 22 0.008242392271933005 0.0 0.0 0.0 0.0016484784543866011 23 0.009890870726319606 0.0 0.0 0.0 0.0016484784543866011 24 0.009890870726319606 0.0 0.0 0.0 0.0016484784543866011 25 0.009890870726319606 0.0 0.0 0.0 0.0016484784543866011 26 0.009890870726319606 0.0 0.0 0.0 0.0016484784543866011 27 0.011539349180706208 0.0 0.0 0.0 0.0016484784543866011 28 0.01483630608947941 0.0 0.0 0.0 0.0016484784543866011 29 0.01483630608947941 0.0 0.0 0.0 0.0016484784543866011 30 0.023078698361412416 0.0 0.0 0.0 0.0016484784543866011 31 0.023078698361412416 0.0 0.0 0.0 0.0016484784543866011 32 0.02472717681579902 0.0 0.0 0.0 0.0016484784543866011 33 0.026375655270185618 0.0 0.0 0.0 0.0016484784543866011 34 0.026375655270185618 0.0 0.0 0.0 0.0016484784543866011 35 0.02802413372457222 0.0 0.0 0.0 0.0016484784543866011 36 0.02802413372457222 0.0 0.0 0.0 0.0016484784543866011 37 0.02967261217895882 0.0 0.0 0.0 0.0016484784543866011 38 0.02967261217895882 0.0 0.0 0.0 0.0016484784543866011 39 0.03132109063334542 0.0 0.0 0.0 0.0016484784543866011 40 0.03132109063334542 0.0 0.0 0.0 0.0016484784543866011 41 0.03461804754211863 0.0 0.0 0.0 0.0016484784543866011 42 0.036266525996505226 0.0 0.0 0.0 0.0016484784543866011 43 0.036266525996505226 0.0 0.0 0.0 0.0016484784543866011 44 0.036266525996505226 0.0 0.0 0.0 0.0016484784543866011 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCGAA 20 3.9720983E-4 50.450672 50 AACAAGC 25 1.9569254E-5 50.45067 50 CCTATGT 15 0.008099801 50.45067 49 GCGACTG 30 9.69274E-7 50.45067 50 GATACAA 15 0.008099801 50.45067 50 TTCAACT 15 0.008099801 50.45067 50 ATTGGTT 15 0.008099801 50.45067 50 TGATACA 15 0.008099801 50.45067 49 GAACAAG 25 1.9569254E-5 50.45067 49 CAGGCTA 15 0.008099801 50.45067 49 CTATGTC 15 0.008099801 50.45067 50 TGGGACT 15 0.008099801 50.45067 49 CAAGAAC 25 1.9666444E-5 50.40852 46 GTCATCA 15 0.008126636 50.40852 46 GGCAATT 15 0.008126636 50.40852 46 TATGTTC 15 0.008126636 50.40852 46 CCTGATA 15 0.008126636 50.40852 47 TGTTCAA 15 0.008126636 50.40852 48 CTGATAC 15 0.008126636 50.40852 48 AGAACAA 25 1.9666444E-5 50.40852 48 >>END_MODULE