FastQCFastQC Report
Tue 31 May 2016
SRR212522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46179
Sequences flagged as poor quality0
Sequence length58
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT22004.764070248381299No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG17063.694319928972044No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11822.55960501526668No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8571.8558219103921696No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6321.3685874531713549No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4961.074081292362329No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.5478680785638494No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG1810.39195305225318866No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1790.3876220793001148No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1670.36163624158167135No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.3334849173866909No Hit
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1400.3031681067151736No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1400.3031681067151736Illumina Single End Adapter 2 (96% over 25bp)
CAAAGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1360.29450616080902575No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1310.28367872842634095No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.22954156651291713No Hit
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1010.21871413413023238No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCGAA990.21438316117715844No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCTTC980.2122176747006215No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.19922475584139976No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAAT810.17540440459949327No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.16674245869334545No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.14941856688104982No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAT680.14725308040451288No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGTGG670.1450875939279759No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT650.14075662097490202No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT600.12992918859221725Illumina Single End Adapter 2 (96% over 32bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT580.12559821563914333No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.12343272916260636No Hit
CAAAGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT570.12343272916260636No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12126724268606943No Hit
CAAAGTGGGATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGG490.10610883735031075No Hit
CAAAGTGGGAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGT480.1039433508737738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTT150.00717653352.00000437
ATCCTCA150.00717653352.00000424
CTTCAGC150.00717653352.00000441
ATGGGCC150.00717653352.00000447
GGATGGT307.827766E-752.0000048
GTATGAG150.00717653352.00000418
TCCTCAA150.00717653352.00000425
GGGCAGC150.00717653352.0000047
GGCATAT150.00717653352.00000446
AGAAGAC150.00717653352.00000423
AAACAGG150.00717653352.00000441
CTGGGCC150.00717653352.00000435
GTGTAAG150.00717653352.00000410
TCTGGGC150.00717653352.00000434
AAGAGAG150.00717653352.00000414
ACCGCGA150.00717653352.00000428
ATGCGTT150.00717653352.00000433
ACTCTTA150.00717653352.00000452
ACACTCT150.00717653352.00000450
GTCGAGT150.00717653352.00000431
Produced by FastQC (version 0.11.5)