FastQCFastQC Report
Tue 31 May 2016
SRR212521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46972
Sequences flagged as poor quality0
Sequence length58
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT22034.6900281018479095No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG17023.623435238014136No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10972.335433875500298No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8521.8138465468789917No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5941.2645831559226774No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4530.9644043259814358No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.6088733713701779No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG1750.37256237758664734No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1710.3640466660989526Illumina Single End Adapter 2 (96% over 25bp)
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1690.3597888103551052No Hit
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1660.3534020267393341No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1430.30443668568508897No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.2874052627096994No Hit
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1060.2256663544239121No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1060.2256663544239121No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.21927957080814103No Hit
CAAAGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1030.21927957080814103No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.21289278719236993No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCGAA870.18521672485736185No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.18308779698543814No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT820.17457208549774333Illumina Single End Adapter 2 (96% over 32bp)
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.17457208549774333No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCTTC780.16605637401004852No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAAT670.14263816741888785No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAT630.13412245593119307No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.13199352805926937No Hit
CAAAGTGGGATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGG610.12986460018734566No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT590.12560674444349826No Hit
CAAAGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT530.11283317721195606No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG480.10218853785233756No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.10005960998041386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGA251.5954134E-552.19251623
CGCGAAG150.007073005752.19251325
TATCGGG150.007073005752.19251325
GCGAAGG150.007073005752.19251326
CATTTGC203.351823E-452.19251327
CTGGCAT203.351823E-452.19251323
CAAAGAC150.007073005752.19251323
AATATCG150.007073005752.19251323
ATATCGG150.007073005752.19251324
ACGCGAA150.007073005752.19251324
TGATGCC203.4233584E-451.97018439
ATGGGAT203.4233584E-451.97018436
TAGTGCG203.4233584E-451.97018437
AGCCAGC203.4233584E-451.97018448
TGCCGAA203.4233584E-451.97018442
ACCCGAG203.4233584E-451.97018443
TATTGCA203.4233584E-451.97018447
TGGGGCC800.051.9701846
TCTGTCT203.4233584E-451.97018418
CGGCGGT401.8389983E-951.97018447
Produced by FastQC (version 0.11.5)