SRR212520_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212520_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49156
Sequences flagged as poor quality	0
Sequence length	58
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2212	4.499959313206933	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1563	3.179672878183742	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	992	2.018064936121735	No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	753	1.5318577589714377	No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	600	1.2206037920091137	No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	392	0.7974611441126209	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	216	0.439417365123281	No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG	186	0.3783871755228253	No Hit
CAAAGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	168	0.34176906176255184	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	153	0.31125396696232405	Illumina Single End Adapter 2 (96% over 25bp)
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	138	0.2807388721620962	No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	133	0.27056717389535356	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	131	0.26649849458865654	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	119	0.24208641874847425	No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCTTC	104	0.21157132394824638	No Hit
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG	102	0.20750264464154933	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.18105622914801855	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.17291887053462446	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	84	0.17088453088127592	No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.16478151192123036	No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81	0.16478151192123036	No Hit
CAAAGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	81	0.16478151192123036	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCGAA	80	0.16274717226788185	No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAAT	66	0.1342664171210025	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGTGG	64	0.1301977378143055	No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAT	60	0.1220603792009114	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT	60	0.1220603792009114	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	59	0.12002603954756287	Illumina Single End Adapter 2 (96% over 32bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT	56	0.1139230205875173	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	54	0.10985434128082025	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCATGC	20	3.323905E-4	52.285423	8
GTGGGTT	40	1.7444108E-9	52.285423	5
GTGGGCG	40	1.7444108E-9	52.285423	5
GGAAGCG	40	1.7444108E-9	52.285423	8
GGGACCA	20	3.323905E-4	52.285423	7
TGGGTTA	20	3.323905E-4	52.285423	6
GGTGGTC	20	3.323905E-4	52.285423	8
TGGGTCT	20	3.323905E-4	52.285423	6
GTGGTTG	20	3.323905E-4	52.285423	9
TGGGATT	75	0.0	52.285423	6
GGATGGT	25	1.5796933E-5	52.28542	8
GGGCACC	15	0.007025191	52.28542	7
GGACCAT	15	0.007025191	52.28542	8
GAAGCGT	15	0.007025191	52.28542	9
GGATTGG	15	0.007025191	52.28542	8
GGAAGTG	35	3.621608E-8	52.28542	8
GCATGCC	15	0.007025191	52.28542	9
GAACAGT	15	0.007025191	52.28542	9
AAGCGAT	15	0.007025191	52.28542	10
TGGGCTC	25	1.5796933E-5	52.28542	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content