FastQCFastQC Report
Tue 31 May 2016
SRR212520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49156
Sequences flagged as poor quality0
Sequence length58
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT22124.499959313206933No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG15633.179672878183742No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9922.018064936121735No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7531.5318577589714377No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6001.2206037920091137No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3920.7974611441126209No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.439417365123281No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG1860.3783871755228253No Hit
CAAAGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1680.34176906176255184No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1530.31125396696232405Illumina Single End Adapter 2 (96% over 25bp)
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1380.2807388721620962No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1330.27056717389535356No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1310.26649849458865654No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.24208641874847425No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCTTC1040.21157132394824638No Hit
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1020.20750264464154933No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.18105622914801855No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.17291887053462446No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT840.17088453088127592No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.16478151192123036No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.16478151192123036No Hit
CAAAGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT810.16478151192123036No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCGAA800.16274717226788185No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCAAT660.1342664171210025No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGTGG640.1301977378143055No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAAT600.1220603792009114No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT600.1220603792009114No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT590.12002603954756287Illumina Single End Adapter 2 (96% over 32bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT560.1139230205875173No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG540.10985434128082025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATGC203.323905E-452.2854238
GTGGGTT401.7444108E-952.2854235
GTGGGCG401.7444108E-952.2854235
GGAAGCG401.7444108E-952.2854238
GGGACCA203.323905E-452.2854237
TGGGTTA203.323905E-452.2854236
GGTGGTC203.323905E-452.2854238
TGGGTCT203.323905E-452.2854236
GTGGTTG203.323905E-452.2854239
TGGGATT750.052.2854236
GGATGGT251.5796933E-552.285428
GGGCACC150.00702519152.285427
GGACCAT150.00702519152.285428
GAAGCGT150.00702519152.285429
GGATTGG150.00702519152.285428
GGAAGTG353.621608E-852.285428
GCATGCC150.00702519152.285429
GAACAGT150.00702519152.285429
AAGCGAT150.00702519152.2854210
TGGGCTC251.5796933E-552.285426