FastQCFastQC Report
Tue 31 May 2016
SRR212519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55338
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT36786.646427409736528No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA14172.5606274169648344No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA11041.99501246882793No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6851.2378474104593589No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5470.9884708518558676No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3890.7029527630199862No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.4698398930210705No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG2440.4409266688351585Illumina Single End Adapter 2 (95% over 23bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGT2400.4336983627886805No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1410.2547977881383498No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG1360.24576240558025225Illumina Single End Adapter 2 (96% over 30bp)
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG1280.23130579348729624No Hit
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1240.22407748744081826No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCT1220.22046333441757926No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.20781379883624274No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.20781379883624274No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.20600672232462322No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGG1110.20058549278976473No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1030.1861288806968087No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT1030.1861288806968087No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA930.16805811558061368No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.16082980953413567No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT890.16082980953413567No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCG800.14456612092956017No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT720.13010950883660413Illumina Single End Adapter 2 (100% over 29bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA700.12649535581336513No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT680.12288120279012613No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCA660.11926704976688712No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC650.11745997325526762No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA640.11565289674364812No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGAAGCAGG630.11384582023202862No Hit
CAAAGTGGGATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGC580.10481043767393113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCGA204.0792412E-450.17197837
TAACGGC204.0792412E-450.17197837
AGAGGAG402.5247573E-950.17197838
GAGCAGG204.0792412E-450.17197850
CGGCGAG204.0792412E-450.17197840
AGCGGGA204.0792412E-450.17197844
CCTTCTC402.5247573E-950.17197850
CCCGAAT204.0792412E-450.17197840
GGTAGGC204.0792412E-450.17197848
CGAAAAG204.0792412E-450.17197839
GAGAGGA402.5247573E-950.17197837
CTTGAAA204.0792412E-450.17197846
AACGGCG204.0792412E-450.17197838
GTAACGG204.0792412E-450.17197836
CCGAATG204.0792412E-450.17197841
GTGCTCG204.0792412E-450.17197836
CGCTGGC150.00827554350.17197450
AGCTCAA355.02896E-850.17197437
CAGCACT150.00827554350.17197442
ACTGACG252.0199428E-550.17197442