SRR212519_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212519_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55338
Sequences flagged as poor quality	0
Sequence length	56
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	3678	6.646427409736528	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1417	2.5606274169648344	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1104	1.99501246882793	No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	685	1.2378474104593589	No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	547	0.9884708518558676	No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	389	0.7029527630199862	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.4698398930210705	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	244	0.4409266688351585	Illumina Single End Adapter 2 (95% over 23bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGT	240	0.4336983627886805	No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	141	0.2547977881383498	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	136	0.24576240558025225	Illumina Single End Adapter 2 (96% over 30bp)
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	128	0.23130579348729624	No Hit
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	124	0.22407748744081826	No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCT	122	0.22046333441757926	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.20781379883624274	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.20781379883624274	No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.20600672232462322	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGG	111	0.20058549278976473	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	103	0.1861288806968087	No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT	103	0.1861288806968087	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.16805811558061368	No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.16082980953413567	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT	89	0.16082980953413567	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCG	80	0.14456612092956017	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	72	0.13010950883660413	Illumina Single End Adapter 2 (100% over 29bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	70	0.12649535581336513	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT	68	0.12288120279012613	No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCA	66	0.11926704976688712	No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	65	0.11745997325526762	No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA	64	0.11565289674364812	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGAAGCAGG	63	0.11384582023202862	No Hit
CAAAGTGGGATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGC	58	0.10481043767393113	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTCGA	20	4.0792412E-4	50.171978	37
TAACGGC	20	4.0792412E-4	50.171978	37
AGAGGAG	40	2.5247573E-9	50.171978	38
GAGCAGG	20	4.0792412E-4	50.171978	50
CGGCGAG	20	4.0792412E-4	50.171978	40
AGCGGGA	20	4.0792412E-4	50.171978	44
CCTTCTC	40	2.5247573E-9	50.171978	50
CCCGAAT	20	4.0792412E-4	50.171978	40
GGTAGGC	20	4.0792412E-4	50.171978	48
CGAAAAG	20	4.0792412E-4	50.171978	39
GAGAGGA	40	2.5247573E-9	50.171978	37
CTTGAAA	20	4.0792412E-4	50.171978	46
AACGGCG	20	4.0792412E-4	50.171978	38
GTAACGG	20	4.0792412E-4	50.171978	36
CCGAATG	20	4.0792412E-4	50.171978	41
GTGCTCG	20	4.0792412E-4	50.171978	36
CGCTGGC	15	0.008275543	50.171974	50
AGCTCAA	35	5.02896E-8	50.171974	37
CAGCACT	15	0.008275543	50.171974	42
ACTGACG	25	2.0199428E-5	50.171974	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content