##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212519_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55338
Sequences flagged as poor quality	0
Sequence length	56
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.7165781199176	38.0	36.0	38.0	33.0	40.0
2	35.80255882034045	38.0	35.0	38.0	30.0	40.0
3	36.054826701362536	38.0	35.0	38.0	30.0	40.0
4	35.91725396653294	38.0	35.0	38.0	30.0	40.0
5	35.9132964689725	38.0	35.0	38.0	30.0	40.0
6	33.788716614261446	36.0	33.0	38.0	26.0	39.0
7	36.7470454299035	38.0	36.0	40.0	33.0	40.0
8	36.45981061838158	38.0	36.0	39.0	31.0	40.0
9	36.225613502475696	38.0	36.0	39.0	31.0	40.0
10	34.76545953955691	38.0	33.0	39.0	27.0	40.0
11	34.22723987133615	38.0	33.0	39.0	26.0	40.0
12	34.45623260688858	38.0	33.0	39.0	26.0	40.0
13	34.340778488561206	38.0	33.0	39.0	26.0	40.0
14	34.253117206982544	38.0	33.0	39.0	26.0	40.0
15	34.46718349054899	38.0	33.0	39.0	26.0	40.0
16	34.893942679533055	38.0	35.0	39.0	28.0	40.0
17	35.19886877010373	38.0	35.0	39.0	28.0	40.0
18	35.04244822725794	38.0	35.0	39.0	28.0	40.0
19	34.077523582348476	38.0	33.0	39.0	25.0	40.0
20	34.682641223029385	38.0	35.0	39.0	27.0	40.0
21	34.40961003288879	38.0	35.0	39.0	26.0	40.0
22	34.21453612345947	38.0	35.0	39.0	25.0	40.0
23	33.81067259387763	38.0	33.0	39.0	23.0	40.0
24	33.75261122555929	38.0	33.0	39.0	23.0	40.0
25	33.69201995012469	38.0	33.0	39.0	23.0	40.0
26	32.85846976760996	38.0	33.0	39.0	13.0	40.0
27	32.77747659835917	38.0	33.0	39.0	10.0	40.0
28	31.867577433228522	36.0	32.0	39.0	2.0	40.0
29	31.988633488741915	37.0	32.0	39.0	2.0	40.0
30	30.94457696338863	36.0	31.0	39.0	2.0	40.0
31	29.58984784415772	36.0	28.0	39.0	2.0	40.0
32	28.739582203910512	35.0	25.0	39.0	2.0	40.0
33	29.139759297408652	36.0	27.0	39.0	2.0	40.0
34	29.14689724962955	36.0	27.0	39.0	2.0	40.0
35	28.29878203043117	35.0	23.0	39.0	2.0	40.0
36	27.530955220644042	35.0	11.0	39.0	2.0	40.0
37	26.945173298637464	35.0	2.0	39.0	2.0	40.0
38	26.791626007445156	35.0	2.0	39.0	2.0	40.0
39	26.8013842206079	35.0	2.0	39.0	2.0	40.0
40	26.482886985434963	35.0	2.0	39.0	2.0	40.0
41	26.434963316346813	35.0	2.0	39.0	2.0	40.0
42	26.40277928367487	35.0	2.0	39.0	2.0	40.0
43	26.115707109038997	35.0	2.0	39.0	2.0	40.0
44	25.892406664498175	35.0	2.0	39.0	2.0	40.0
45	25.79008999241028	35.0	2.0	39.0	2.0	40.0
46	25.63406700639705	35.0	2.0	39.0	2.0	40.0
47	25.27930174563591	35.0	2.0	39.0	2.0	40.0
48	25.282699049477756	35.0	2.0	39.0	2.0	40.0
49	25.10634645270881	34.0	2.0	39.0	2.0	40.0
50	24.850789692435576	34.0	2.0	39.0	2.0	40.0
51	24.36674617803318	34.0	2.0	39.0	2.0	40.0
52	24.02753984603708	34.0	2.0	39.0	2.0	40.0
53	23.605298348332067	33.0	2.0	38.0	2.0	40.0
54	23.381853337670318	33.0	2.0	38.0	2.0	39.0
55	22.817051573963642	33.0	2.0	38.0	2.0	40.0
56	12.747226137554664	16.0	2.0	21.0	2.0	25.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.6392977834302158
1	2	-1.153470101423217
1	3	-1.4817120601396354
1	4	-1.3589926047263248
1	5	-2.650469161602942
1	6	-3.015283898558046
1	7	-2.411344934396638
1	8	-1.9781247160548503
1	9	-1.956066722661589
1	10	-3.803123591380519
1	11	-3.8185876808544883
1	12	-4.014748297819871
1	13	-3.3481512533075204
1	14	-3.4364316027579704
1	15	-3.5271369789120257
1	16	-2.5287065296441185
1	17	-2.6269677117362065
1	18	-2.7920295344089823
1	19	-2.8315010834671597
1	20	-1.7639287973505446
1	21	-2.2345345982655367
1	22	-3.213556619842443
1	23	-2.52995907050947
1	24	-2.922720654666829
1	25	-3.7880243678293475
1	26	-4.488414310399637
1	27	-3.482421998711345
1	28	-2.4746261981218787
1	29	-2.6823060138668
1	30	-2.4173316707020156
1	31	-2.374475016953216
1	32	-1.8730413092399871
1	33	-1.6111940744085054
1	34	-2.036137913601255
1	35	-2.646520881939619
1	36	-0.6374546478699301
1	37	-0.39399705842780364
1	38	0.07886158265811716
1	39	-0.6657492877602458
1	40	-0.0580423802149852
1	41	0.35195445427912375
1	42	0.4756435980921232
1	43	0.4911594999376092
1	44	0.8713882295033102
1	45	0.40682725919042895
1	46	0.2983510133980687
1	47	-0.7510323449236935
1	48	-0.5256215613619943
1	49	-1.1477571475826487
1	50	-0.8411716401860403
1	51	0.1421632900933183
1	52	-0.4489013078802877
1	53	0.41783838426684383
1	54	-0.8557786134936975
1	55	0.3256110770221099
1	56	0.537476918579971
2	1	-0.4338432379756725
2	2	-0.2134701014232192
2	3	-0.6135302419578181
2	4	-0.29717442290814233
2	5	0.4295308383970564
2	6	0.3192615559874099
2	7	-0.22770857076027085
2	8	-0.36812471605485086
2	9	-0.44152126811613357
2	10	-1.1276690459259697
2	11	-2.532224044490853
2	12	-2.519293752365325
2	13	-3.1808785260347925
2	14	-2.0709770573034234
2	15	-3.7971369789120253
2	16	-3.3705247114622985
2	17	-2.4815131662816583
2	18	-1.914756807136257
2	19	-2.1315010834671604
2	20	-2.5312015246232704
2	21	-1.7408982346291708
2	22	-2.1008293471151696
2	23	-1.4699590705094678
2	24	-1.8363570183031932
2	25	-1.4298425496475318
2	26	-0.8020506740359998
2	27	-1.5851492714386168
2	28	-1.333717107212788
2	29	-2.270487832048616
2	30	-2.819149852520198
2	31	-5.291747744225944
2	32	-3.84576858196726
2	33	-4.517557710772142
2	34	-3.8706833681467074
2	35	-4.149248154666893
2	36	-4.315636466051746
2	37	-2.9658152402459876
2	38	-3.419320235523699
2	39	-3.199385651396611
2	40	-3.104406016578622
2	41	-3.032591000266329
2	42	-2.525265492816967
2	43	-2.977022318244206
2	44	-2.2677026795876003
2	45	-2.5722636499004814
2	46	-3.0080126229655697
2	47	-3.1683050721964214
2	48	-2.6847124704529044
2	49	-2.1941207839462855
2	50	-2.404808003822403
2	51	-1.5178367099066818
2	52	-1.1143558533348354
2	53	-2.896707070278609
2	54	-2.715778613493697
2	55	-2.6707525593415262
2	56	-1.7325230814200285
3	1	-0.20922785336028227
3	2	-0.6534701014232169
3	3	-1.1442994727270488
3	4	-0.1771744229081449
3	5	-0.7081614692952485
3	6	-0.27227690555105255
3	7	0.06767604462434207
3	8	-0.016586254516390397
3	9	0.32540180880694436
3	10	-0.08459212284905249
3	11	1.875468263201455
3	12	1.4153216322500626
3	13	0.8598907047344397
3	14	0.10902294269657631
3	15	0.10747840570336109
3	16	-0.40283240376999174
3	17	-0.6584362432047399
3	18	-0.10321834559779575
3	19	-0.4084241603902399
3	20	0.43187539845364853
3	21	0.032947919216979926
3	22	0.1360937298079108
3	23	-0.2799590705094701
3	24	-0.4402031721493458
3	25	-0.4313810111859908
3	26	-0.12128144326676704
3	27	-0.44745696374631194
3	28	0.23166750817182802
3	29	0.5379737064129237
3	30	-0.11838062175096908
3	31	-0.23636312884132593
3	32	0.5850006488019694
3	33	0.5024422892278579
3	34	0.701624324160985
3	35	1.1384441530254144
3	36	0.04436353394825332
3	37	0.23187706744631953
3	38	0.3568336106301473
3	39	0.6436912716803143
3	40	0.2479016757290715
3	41	0.5897166920413639
3	42	0.019349891798416508
3	43	0.36528537406348605
3	44	0.15460501272009353
3	45	-0.3484174960543278
3	46	0.18121814626520205
3	47	0.8870795431881966
3	48	0.8729798372394022
3	49	1.2351099852844847
3	50	0.7567304577160563
3	51	1.6806248285548584
3	52	0.6017979928190123
3	53	0.655600622029084
3	54	1.3942213865063025
3	55	1.2661705175815499
3	56	1.152861533964586
4	1	-0.046673426654912475
4	2	-0.08743236557415912
4	3	-0.2531528834672514
4	4	0.1907501053937395
4	5	-0.06556350122558285
4	6	-0.42866297231447703
4	7	-0.019406683967815752
4	8	0.2058375480960919
4	9	0.48904476961971
4	10	-1.3378577251712542
4	11	-0.5737334784531143
4	12	-0.9551428089691001
4	13	-1.724652110940454
4	14	-0.19286384975625737
4	15	-0.635627544949763
4	16	-1.5407133907075803
4	17	-1.2890603360929802
4	18	-1.6638134109098388
4	19	-1.9635765551652717
4	20	-1.2270505812270471
4	21	-1.4416529516103083
4	22	-1.242716139568003
4	23	-2.4356194478679605
4	24	-2.2533381503786636
4	25	-2.601918021345643
4	26	-2.0548808627152475
4	27	-1.9013756865329583
4	28	-1.5205095600429779
4	29	-2.146714247142956
4	30	-3.0897158902560484
4	31	-1.4460873668674523
4	32	-1.155202544231411
4	33	-1.695670918319312
4	34	-2.2737022360712373
4	35	-1.7730217395725525
4	36	-3.946202503787596
4	37	-2.127702032698817
4	38	-2.663848537410491
4	39	-2.930329047623026
4	40	-2.1425192241257918
4	41	-2.4846664719644416
4	42	-2.3275296437603643
4	43	-2.5109845823951495
4	44	-2.8254385286442023
4	45	-1.9598108197118016
4	46	-2.154050358814626
4	47	-2.681135260875667
4	48	-2.7696181308302634
4	49	-3.4869509726255323
4	50	-3.025562720803535
4	51	-3.5842518042463034
4	52	-3.4094501929574754
4	53	-3.1216127306559684
4	54	-3.035023896512566
4	55	-2.4333940687754883
4	56	-1.3917683644388976
5	1	0.575968082779049
5	2	1.5729449929164048
5	3	1.1996773052119991
5	4	1.228485954450349
5	5	1.3872666874536606
5	6	1.4581294805157086
5	7	1.1126687877302928
5	8	1.2435733971526943
5	9	1.3569692979216015
5	10	1.5678026521872326
5	11	1.954568408339334
5	12	2.384479832540336
5	13	2.2187441154746423
5	14	2.3543059615644992
5	15	2.0058818890124996
5	16	2.081928118726381
5	17	2.069430229944757
5	18	2.5059979098448792
5	19	1.7534045769101994
5	20	2.2446475319805046
5	21	2.5583470483896917
5	22	1.681812162318792
5	23	2.6964560238301516
5	24	2.576850528866622
5	25	2.266006506956245
5	26	3.0583266844545633
5	27	2.8156054455425163
5	28	2.856848930523057
5	29	3.3438517905928897
5	30	2.3631142984231985
5	31	2.9124031991702815
5	32	2.56177858784406
5	33	2.6250837986618194
5	34	2.933844933740083
5	35	2.6420726000500885
5	36	3.1481371188539136
5	37	2.92890174088609
5	38	2.864453349381961
5	39	3.069670952376974
5	40	2.838612851345907
5	41	3.326654282752539
5	42	3.578130733598126
5	43	2.9229776817557926
5	44	3.91041052795957
5	45	3.794906161420272
5	46	3.7893458676004705
5	47	3.1490534183696184
5	48	4.192646020113134
5	49	3.2111622349216375
5	50	3.634814637687029
5	51	4.208201025942376
5	52	3.515078108929316
5	53	4.029330665570448
5	54	3.7951647827327193
5	55	4.000568195375454
5	56	1.6459674846177066
6	1	-0.4338432379756725
6	2	-0.6734701014232201
6	3	-0.5735302419578119
6	4	-0.5571744229081474
6	5	-0.19046916160294103
6	6	-0.6807384440125901
6	7	-0.32770857076027227
6	8	-0.7281247160548503
6	9	-0.16152126811613243
6	10	-2.3076690459259694
6	11	-1.4522240444908547
6	12	-1.2992937523653225
6	13	-1.0608785260347915
6	14	-1.730977057303427
6	15	-2.6971369789120274
6	16	-3.4905247114622995
6	17	-4.261513166281659
6	18	-2.974756807136256
6	19	-2.9515010834671607
6	20	-3.4712015246232717
6	21	-3.020898234629172
6	22	-3.800829347115169
6	23	-3.329959070509471
6	24	-2.736357018303192
6	25	-3.049842549647529
6	26	-2.2220506740360015
6	27	-1.76514927143862
6	28	-1.4337171072127894
6	29	-1.8304878320486146
6	30	-1.9991498525201976
6	31	-1.5317477442259424
6	32	-1.905768581967262
6	33	-2.657557710772142
6	34	-1.6106833681467094
6	35	-1.7692481546668937
6	36	-1.9156364660517475
6	37	-2.0258152402459864
6	38	-2.2393202355236994
6	39	-1.4393856513966092
6	40	-2.2844060165786217
6	41	-2.0125910002663296
6	42	-1.7852654928169684
6	43	-1.3170223182442058
6	44	-1.567702679587601
6	45	-1.47226364990048
6	46	-1.3880126229655687
6	47	-2.3883050721964203
6	48	-1.8047124704529054
6	49	-2.4141207839462844
6	50	-1.8848080038224033
6	51	-1.1578367099066824
6	52	-0.894355853334833
6	53	-1.2167070702786091
6	54	-0.5157786134936977
6	55	-0.7307525593415285
6	56	-1.4325230814200296
7	1	0.40615676202433093
7	2	0.21609511596808773
7	3	0.12994801891174745
7	4	-0.2641309446472775
7	5	0.3495308383970581
7	6	0.5914354690308841
7	7	0.5827262118484242
7	8	-0.03247254214180373
7	9	0.6289135144925595
7	10	0.18798312798707428
7	11	0.763428129422195
7	12	0.47635842154772234
7	13	0.6834693000521597
7	14	0.8264142470444042
7	15	0.2228630210879743
7	16	-0.688785581027517
7	17	-0.9945566445425271
7	18	-1.5321481114840836
7	19	-1.135848909554113
7	20	-1.0129406550580526
7	21	-1.381767799846564
7	22	-0.636481521028216
7	23	-0.356046027031212
7	24	-0.12331354004232509
7	25	-0.27419037573448435
7	26	0.15881889118139014
7	27	-0.6355840540473139
7	28	0.17063071887416825
7	29	0.48864260273399296
7	30	0.03128493008849631
7	31	-0.786530352921595
7	32	-0.08055119066291283
7	33	-0.8236446672938804
7	34	-0.773292063798884
7	35	-2.147509024232111
7	36	-0.80346255300827
7	37	-1.1953804576372917
7	38	-1.00018980074109
7	39	-1.3228639122661754
7	40	-1.3574494948394928
7	41	-0.7438953480924155
7	42	-0.604395927599576
7	43	-1.4031092747659457
7	44	-1.0703113752397755
7	45	-0.7305245194656997
7	46	-0.06627349253078663
7	47	0.22821666693401355
7	48	-0.554277687844209
7	49	0.18327052040153902
7	50	-0.8787210473006652
7	51	-0.29261931860233403
7	52	-0.7334862881174438
7	53	-0.5367070702786094
7	54	0.14422138650630245
7	55	0.03707352761499649
7	56	-0.4951317770722028
8	1	0.23307983894741113
8	2	0.6734529754998633
8	3	0.06723898881141821
8	4	0.7651332693995485
8	5	0.311069299935518
8	6	0.36233847906432715
8	7	0.18306066000896237
8	8	-0.3435093314394635
8	9	-0.25152126811613584
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114	25	-0.6535160190352869
114	26	-0.31633638832171584
114	27	0.8826058306021949
114	28	0.5907726887055773
114	29	-1.036202117762901
114	30	-1.4358845463977516
114	31	-1.086441621776963
114	32	-2.135564500334606
114	33	-2.017965874037447
114	34	-1.8070098987589525
114	35	-0.9243501954832176
114	36	-1.9250242211537874
114	37	-0.9890805463684345
114	38	-2.392381460013496
114	39	-1.6977529983353854
114	40	-1.3627733635173982
114	41	0.4841436936112231
114	42	-0.28363283975574305
114	43	0.004610334817016337
114	44	0.008215687759339119
114	45	-0.1400187519412981
114	46	0.840558805605859
114	47	0.9145520706607222
114	48	0.039777325465461644
114	49	-0.870855477823838
114	50	-0.010930452801996893
114	51	-0.05171426092708842
114	52	-0.28007013904911915
114	53	0.5449255827826143
114	54	0.2768744477307905
114	55	0.3525127467809206
114	56	0.7568646736820117
115	1	0.2539828489808542
115	2	-0.04477444924930296
115	3	0.04299149717262196
115	4	0.0836951423092458
115	5	-0.020034378994246538
115	6	0.06969633859610269
115	7	-0.04770857076027113
115	8	-0.5107334117070224
115	9	-0.4363038768117846
115	10	-0.6163646980998863
115	11	-1.171354479273461
115	12	-0.13233723062619163
115	13	-0.14261765646957514
115	14	0.4568490296530996
115	15	-0.733658718042463
115	16	-1.8192203636362123
115	17	-1.7336870793251364
115	18	-1.227800285397123
115	19	-3.2228054312932457
115	20	-2.186853698536318
115	21	-2.186115625933521
115	22	-2.0712641297238648
115	23	-2.1603938531181655
115	24	-1.5798352791727588
115	25	-1.469842549647531
115	26	-1.819441978383825
115	27	-2.352975358395142
115	28	-2.677195368082355
115	29	-3.402661745092093
115	30	-4.36001941773759
115	31	-4.830008613791161
115	32	-6.167507712402042
115	33	-4.410601189033013
115	34	-5.099379020320622
115	35	-4.886639459014717
115	36	-4.173027770399571
115	37	-3.8040761098112057
115	38	-3.391494148567176
115	39	-3.7141682600922614
115	40	-3.792232103535145
115	41	-3.135199695918505
115	42	-2.2783089710778377
115	43	-2.229196231287684
115	44	-2.722485288283252
115	45	-2.360959302074395
115	46	-2.8271430577481773
115	47	-2.8587398548051155
115	48	-3.2716689921920334
115	49	-3.5775990448158517
115	50	-2.2048080038224036
115	51	-3.3143584490371154
115	52	-3.12479063594353
115	53	-2.7758375050612187
115	54	-2.3123003526241312
115	55	-2.6151003854284838
115	56	-2.190783950985246
116	1	-0.13230477643720917
116	2	0.19268374473062977
116	3	0.3557005272729512
116	4	-0.04255903829275809
116	5	0.08030006916629162
116	6	0.40080001752586725
116	7	1.0484452753935756
116	8	1.0218752839451497
116	9	1.2292479626530977
116	10	1.146177107920181
116	11	0.7985451862783819
116	12	0.5499370168654423
116	13	0.552198397042126
116	14	1.6282537119273428
116	15	0.819016867241821
116	16	0.02024451930693516
116	17	-0.3699747047431998
116	18	0.31985857747913116
116	19	-0.23534723731331297
116	20	0.7011061676844221
116	21	-0.2555136192445602
116	22	-0.7292908855767095
116	23	-1.02995907050947
116	24	-0.267126249072426
116	25	-0.6429194727244507
116	26	-0.5251275971129203
116	27	-0.9859185022078485
116	28	-0.4029478764435588
116	29	-0.577410908971693
116	30	-0.9260729294432757
116	31	-1.1209785134567127
116	32	-0.0880762742749539
116	33	0.6178269046124747
116	34	-0.7406833681467084
116	35	-1.3807866162053521
116	36	-2.0133287737440533
116	37	-2.556584471015217
116	38	-2.4508586970621593
116	39	-2.817847189858149
116	40	-3.5020983242709285
116	41	-3.756437154112483
116	42	-3.30757318512466
116	43	-4.038560779782667
116	44	-4.6146257565106765
116	45	-3.983032880669711
116	46	-3.5303203152732614
116	47	-4.26676661065796
116	48	-2.7231740089144445
116	49	-2.9956592454847453
116	50	-3.1855772345916336
116	51	-3.8193751714451416
116	52	-3.7635866225656045
116	53	-4.113630147201686
116	54	-4.1057786134936975
116	55	-4.9069064054953735
116	56	-2.097138466035414
117	1	0.09243127182825361
117	2	0.1896671534787444
117	3	-0.7566674968597766
117	4	0.1071393025820484
117	5	-0.04262602434803853
117	6	-1.0313266793067086
117	7	0.04052672335737384
117	8	-0.38498746115288895
117	9	0.1749493201191612
117	10	-1.1155121831808756
117	11	-0.9126162013535932
117	12	-0.43498002687513093
117	13	0.33010186612207093
117	14	-0.38117313573479805
117	15	-0.21831344950025766
117	16	-0.6517011820505303
117	17	-0.9442582643208723
117	18	-0.48142347380291994
117	19	-1.0079716717024567
117	20	-1.3931623089369936
117	21	-1.571878626786038
117	22	-1.4114175824092854
117	23	-0.7456453450192768
117	24	-1.1834158418326055
117	25	-0.17572490258870488
117	26	-0.7196977328595295
117	27	-0.4071100557523408
117	28	-2.6533249503500436
117	29	-2.2210760673427323
117	30	-2.5403263231084345
117	31	-3.9988065677553557
117	32	-3.7634156407907895
117	33	-3.448538102929003
117	34	-4.382840230891805
117	35	-3.6986599193727763
117	36	-3.2595580346791984
117	37	-3.7669917108342226
117	38	-4.053045725719777
117	39	-5.034287612180922
117	40	-5.267935428343328
117	41	-4.411414529678094
117	42	-6.149579218307164
117	43	-5.449571337852049
117	44	-5.042604640371913
117	45	-5.418930316567149
117	46	-5.097816544534197
117	47	-4.738108993765049
117	48	-4.792555607707808
117	49	-4.759610980024718
117	50	-4.645984474410639
117	51	-4.828424945200798
117	52	-5.033571539609344
117	53	-4.438667854592335
117	54	-5.218523711532914
117	55	-5.124478049537604
117	56	-2.8693858265180676
118	1	-0.08403931640704343
118	2	0.8367259770081574
118	3	0.6943128952970881
118	4	0.08753145944479712
118	5	0.3887465246715678
118	6	-0.24701295381651533
118	7	-0.11633602174066482
118	8	-0.05165412781955325
118	9	-0.4132859739984909
118	10	-0.60570826161225
118	11	-0.01065541703987094
118	12	0.4081572280268304
118	13	0.19284696416128355
118	14	-0.616467253381856
118	15	-0.13988207695123833
118	16	-0.2007207898936727
118	17	-0.3560229702032274
118	18	-0.3245607287048813
118	19	-0.027579514839707997
118	20	0.5872298479257481
118	21	0.48694490262573
118	22	0.8238765352377726
118	23	0.8818056353728849
118	24	0.2479567071870008
118	25	0.21643196015639177
118	26	1.1626552083169415
118	27	0.3575958266005941
118	28	1.2682436771009336
118	29	1.5240219718729513
118	30	0.1851638729699978
118	31	0.6090365694995477
118	32	-0.3320430917711832
118	33	-0.15442045587018072
118	34	0.18578722008858506
118	35	0.2817322374899689
118	36	-0.416420779777237
118	37	-0.39444269122638076
118	38	0.24107192133904576
118	39	-0.09311114159268641
118	40	0.1242214344017718
118	41	1.4317227252238673
118	42	0.8112050954183267
118	43	0.2759188582263796
118	44	0.996611045902597
118	45	0.5026383108838317
118	46	0.45120306330894167
118	47	0.3795380650584832
118	48	0.6584247844490569
118	49	0.24038901997528228
118	50	0.4912704275501447
118	51	0.328437799897241
118	52	0.31936963686124287
118	53	0.4240772434468809
118	54	1.1736331512121865
118	55	-0.04604667698858478
118	56	0.3266926048544807
119	1	0.46384906971663753
119	2	0.38499143703832317
119	3	0.3172389888114182
119	4	0.9189794232457018
119	5	0.3687616076278246
119	6	0.7277230944489474
119	7	-0.37463164768335133
119	8	0.1564906685605365
119	9	-0.32844434503921605
119	10	-1.2384382766952058
119	11	-1.6629932752600851
119	12	-1.7000629831345577
119	13	-1.1401092952655603
119	14	-1.4102078265341902
119	15	-1.0463677481427922
119	16	-1.7297554806930648
119	17	-1.9661285508970465
119	18	-1.8724491148285622
119	19	-2.1199626219286998
119	20	-1.6450476784694246
119	21	-1.5439751577060932
119	22	-1.0177524240382496
119	23	-1.52995907050947
119	24	-1.517126249072426
119	25	-2.431381011185991
119	26	-2.6212814432667706
119	27	-3.0436108099001586
119	28	-2.6337171072127887
119	29	-3.6351032166639996
119	30	-4.272226775597122
119	31	-4.870978513456713
119	32	-4.4149993511980306
119	33	-5.074480787695219
119	34	-5.221452598915938
119	35	-4.976940462359199
119	36	-5.494098004513287
119	37	-5.595046009476757
119	38	-4.989320235523699
119	39	-5.298616420627379
119	40	-6.040559862732469
119	41	-6.487206384881713
119	42	-6.192188569740043
119	43	-6.807791549013437
119	44	-5.8646257565106765
119	45	-7.271494419131251
119	46	-6.126474161427108
119	47	-5.785997379888727
119	48	-5.511635547375981
119	49	-6.130274630100132
119	50	-5.858654157668557
119	51	-6.377067479137452
119	52	-7.167432776411758
119	53	-6.382860916432456
119	54	-6.0865478442629275
119	55	-5.887675636264603
119	56	-2.5394461583431056
120	1	-1.343843237975669
120	2	-1.8125610105141234
120	3	-1.390802969230542
120	4	-0.7908107865445118
120	5	-2.7413782525120354
120	6	-1.5607384440125927
120	7	-1.5022540253057244
120	8	-2.023579261509397
120	9	-1.706066722661589
120	10	-0.5303963186532457
120	11	-0.13676949903630486
120	12	0.14434261127104264
120	13	-0.3481512533075204
120	14	-0.11824978457615032
120	15	-0.9135006152756588
120	16	-0.6650701660077516
120	17	-0.7178768026452929
120	18	-0.9056658980453491
120	19	-0.7633192652853396
120	20	-0.036656070077818015
120	21	-1.2118073255382669
120	22	-0.9635566198424428
120	23	-2.166322706873107
120	24	-2.399993381939556
120	25	-1.9471152769202575
120	26	-2.079323401308727
120	27	-2.527876544165892
120	28	-1.2928080163036988
120	29	-1.068669650230433
120	30	-2.849149852520199
120	31	-2.0790204714986693
120	32	-0.9184958546945339
120	33	-1.247557710772142
120	34	-1.8997742772376185
120	35	-0.5556117910305289
120	36	-1.3874546478699301
120	37	-0.8258152402459871
120	38	-1.6484111446146095
120	39	-1.5521129241238825
120	40	-0.7625878347604385
120	41	0.12468172700639713
120	42	-1.4107200382715135
120	43	-0.963385954607844
120	44	-0.49224813413305313
120	45	-0.9113545589913912
120	46	-0.5425580775110213
120	47	0.08987674598539641
120	48	-0.04834883408926771
120	49	0.30678830696280457
120	50	1.2042829052685065
120	51	0.21034510827513486
120	52	0.6420077830288022
120	53	0.41783838426684383
120	54	0.9169486592335758
120	55	1.4165201679311998
120	56	1.6738405549436077
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	420.0
3	40.0
4	154.0
5	71.0
6	263.0
7	653.0
8	312.0
9	307.0
10	425.0
11	390.0
12	450.0
13	632.0
14	532.0
15	582.0
16	734.0
17	790.0
18	918.0
19	1122.0
20	1438.0
21	1292.0
22	894.0
23	1214.0
24	1138.0
25	1290.0
26	829.0
27	776.0
28	835.0
29	1021.0
30	1291.0
31	1548.0
32	1871.0
33	2290.0
34	2926.0
35	3597.0
36	4915.0
37	6885.0
38	7054.0
39	3439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	36.77219993494524	50.09938920813908	6.322960714156638	6.805450142759044
11	25.134627200115656	48.65372799884347	16.35765658317973	9.853988217861144
12	60.60211789367161	15.627597672485452	13.012757960172033	10.757526473670895
13	17.55574831038346	21.650583685713254	21.464454805016445	39.32921319888684
14	19.391738046188873	50.44092666883516	18.238823231775633	11.928512053200333
15	46.55751924536485	17.01181828038599	24.5364848747696	11.894177599479562
16	14.098810943655355	17.716578119917596	50.72463768115942	17.459973255267627
17	43.026491741660344	16.406447648993456	15.242690375510499	25.3243702338357
18	43.302974447938126	20.555495319671834	16.865445082944813	19.276085149445226
19	16.153456937366727	17.73826303805703	53.49669304998373	12.611586974592504
20	18.265929379449926	43.846904477935595	19.389930969677255	18.49723517293722
21	17.91535653619574	21.386750515016807	44.09266688351585	16.6052260652716
22	21.43915573385377	24.541906104304456	13.262134518775525	40.75680364306625
23	22.696880985940947	51.39325599045863	13.744623947377931	12.165239076222486
24	17.01543243340923	55.459178141602514	16.56727745852759	10.95811196646066
25	14.110884144688082	26.618340895533183	20.157631193622443	39.11314376615629
26	44.28509422360473	21.454407349802874	15.898650848193293	18.361847578399104
27	17.67930809871897	18.48085691539408	19.13946587537092	44.70036911051603
28	19.70826697553207	47.707036339944985	16.7456927754452	15.839003909077748
29	41.85857049750163	22.635599971033386	22.22282569338837	13.283003838076619
30	16.093682075061587	46.95334009563831	25.119547891609912	11.833429937690191
31	38.8141333285039	23.70103410181647	25.76380462538711	11.72102794429252
32	20.812642719924607	24.92297654862445	43.6623291891696	10.602051542281345
33	34.783002791776944	21.793988615351147	21.672528189695804	21.750480403176102
34	24.278745897328957	22.8607177180989	24.918853246776795	27.941683137795348
35	15.778495247768992	44.12682289777262	28.51882754117391	11.57585431328448
36	26.875340228633643	28.724369442932318	21.912538559245146	22.487751769188897
37	16.550810388950396	26.11757445958945	39.88057425994156	17.451040891518595
38	23.064773800014525	24.684118800377604	33.63590153220536	18.615205867402512
39	18.257886521218282	23.41634297745671	32.174102443097254	26.151668058227756
40	19.541920953211275	25.864574251671023	41.208950886370246	13.384553908747456
41	17.69246136243939	25.33461671176652	37.77491237309991	19.198009552694188
42	19.999636436349093	31.1028703350239	36.00189053098472	12.895602697642289
43	18.47280791042606	32.717754834957105	34.07372400756144	14.7357132470554
44	16.74421986331249	34.58630216664243	37.42547622509815	11.244001744946925
45	25.926127993889462	28.08118282503137	32.81321039518431	13.17947878589485
46	20.041465100207326	28.303568180991522	36.587494998726946	15.067471720074202
47	23.50372136189107	26.708277983003658	34.485833348497806	15.302167306607464
48	27.689366351056083	25.82119446467589	33.441369264384555	13.048069919883467
49	26.559572106900504	25.260610912001745	34.649880837593464	13.529936143504283
50	15.489085910903167	30.60314223816199	37.83065411713303	16.077117733801817
51	19.98579985071638	32.46918749658651	33.92561306413734	13.619399588559777
52	23.445525079226314	28.696317342366957	36.58252285724693	11.2756347211598
53	14.750992750191264	31.427374403439106	38.387555102189516	15.434077744180117
54	14.942319257895791	35.20256601847971	34.24759891381604	15.607515809808458
55	23.243105395460674	27.24142956027542	35.92480600386171	13.590659040402201
56	20.760079433037586	30.787589498806682	35.42057607170836	13.031754996447376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	147.5
9	295.0
10	236.0
11	177.0
12	145.5
13	108.0
14	102.0
15	169.0
16	236.0
17	334.0
18	432.0
19	392.5
20	353.0
21	445.5
22	651.5
23	765.0
24	952.5
25	1140.0
26	1244.5
27	1349.0
28	1783.0
29	2684.0
30	3151.0
31	2529.0
32	1907.0
33	3665.0
34	5423.0
35	3507.5
36	1592.0
37	1445.5
38	1277.5
39	1256.0
40	1319.5
41	1383.0
42	1391.0
43	1399.0
44	1744.5
45	2090.0
46	1914.5
47	1730.0
48	1721.0
49	2361.5
50	3002.0
51	3912.5
52	4823.0
53	4458.5
54	3809.0
55	3524.0
56	3179.5
57	2835.0
58	2509.5
59	2184.0
60	1779.0
61	1374.0
62	1425.0
63	1084.5
64	693.0
65	480.5
66	268.0
67	219.5
68	171.0
69	134.0
70	97.0
71	78.5
72	41.0
73	22.0
74	20.0
75	18.0
76	12.5
77	7.0
78	6.0
79	5.0
80	5.0
81	3.0
82	1.0
83	0.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.03433445372077054
26	0.07951136651125809
27	0.12649535581336513
28	0.14818027395279915
29	0.1843218041851892
30	0.23491994651053524
31	0.21865625790595974
32	0.28913224185912034
33	0.31804546604503237
34	0.3451516137193249
35	0.3722577613936174
36	0.41201344464924644
37	0.43550543930029995
38	0.45899743395135345
39	0.4409266688351585
40	0.5095955762766996
41	0.4969460406953631
42	0.5909140192995771
43	0.5818786367414797
44	0.5818786367414797
45	0.6342838555784452
46	0.6360909320900647
47	0.6957244569735083
48	0.7553579818569518
49	0.6704253858108352
50	0.7390942932523763
51	0.7390942932523763
52	0.7806570530196248
53	0.7951136651125809
54	0.8439047309263074
55	0.7951136651125809
56	0.8113773537171564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	55338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.0612598937439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91835097850498	34.20976544146879
2	10.984119345524542	9.899165130651632
3	4.387231312159128	5.9308251111352055
4	2.5064164260506896	4.517691279048755
5	1.4517163939685596	3.2708084860312985
6	1.082771896053898	2.9274639488235934
7	0.6817452678857876	2.1504210488272073
8	0.5774783445620789	2.081752141385666
9	0.47321142123837023	1.9191152553399111
>10	1.7965992941931346	13.370559109472696
>50	0.06015399422521655	1.875745419061043
>100	0.06015399422521655	4.418302070909682
>500	0.008020532563362208	2.2263182623152264
>1k	0.012030798845043312	11.202067295529293
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	3678	6.646427409736528	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1417	2.5606274169648344	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1104	1.99501246882793	No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	685	1.2378474104593589	No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	547	0.9884708518558676	No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	389	0.7029527630199862	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.4698398930210705	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	244	0.4409266688351585	Illumina Single End Adapter 2 (95% over 23bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGT	240	0.4336983627886805	No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	141	0.2547977881383498	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	136	0.24576240558025225	Illumina Single End Adapter 2 (96% over 30bp)
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	128	0.23130579348729624	No Hit
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	124	0.22407748744081826	No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCT	122	0.22046333441757926	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.20781379883624274	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.20781379883624274	No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.20600672232462322	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGG	111	0.20058549278976473	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	103	0.1861288806968087	No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT	103	0.1861288806968087	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.16805811558061368	No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.16082980953413567	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT	89	0.16082980953413567	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCG	80	0.14456612092956017	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	72	0.13010950883660413	Illumina Single End Adapter 2 (100% over 29bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	70	0.12649535581336513	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT	68	0.12288120279012613	No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCA	66	0.11926704976688712	No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	65	0.11745997325526762	No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA	64	0.11565289674364812	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGAAGCAGG	63	0.11384582023202862	No Hit
CAAAGTGGGATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGC	58	0.10481043767393113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.03614153023239004	0.0	0.0	0.0	0.0
11	0.07770428999963859	0.0	0.0	0.0	0.0
12	0.0831255195344971	0.0	0.0	0.0	0.0
13	0.0831255195344971	0.0	0.0	0.0	0.0
14	0.10481043767393111	0.0	0.0	0.0	0.0
15	0.10481043767393111	0.0	0.0	0.0	0.0
16	0.10661751418555061	0.0	0.0	0.0	0.0
17	0.10661751418555061	0.0	0.0	0.0	0.0
18	0.11203874372040912	0.0	0.0	0.0	0.0
19	0.11565289674364812	0.0	0.0	0.0	0.0
20	0.15360150348765766	0.0	0.0	0.0	0.0
21	0.16444396255737467	0.0	0.0	0.0	0.0
22	0.1879359572084282	0.0	0.0	0.0	0.0
23	0.20419964581300373	0.0	0.0	0.0	0.0
24	0.22949871697567675	0.0	0.0	0.0	0.0
25	0.24576240558025228	0.0	0.0	0.0	0.0
26	0.24937655860349128	0.0	0.0	0.0	0.0
27	0.2566048646499693	0.0	0.0	0.0	0.0
28	0.460804510462973	0.0	0.0	0.0	0.0
29	0.4662257399978315	0.0	0.0	0.0	0.0
30	0.5439300299974701	0.0	0.0	0.0	0.0
31	0.5493512595323286	0.0	0.0	0.0	0.0
32	0.5493512595323286	0.0	0.0	0.0	0.0
33	0.5529654125555676	0.0	0.0	0.0	0.0
34	0.5529654125555676	0.0	0.0	0.0	0.0
35	0.8366764248798294	0.0	0.0	0.0	0.0
36	0.8529401134844049	0.0	0.0	0.0	0.0
37	0.8583613430192635	0.0	0.0	0.0	0.0
38	0.8637825725541219	0.0	0.0	0.0	0.0
39	0.8692038020889804	0.0	0.0	0.0	0.0
40	0.8728179551122195	0.0	0.0	0.0	0.0
41	0.878239184647078	0.0	0.0	0.0	0.0
42	0.8872745672051755	0.0	0.0	0.0	0.0
43	0.8945028732516535	0.0	0.0	0.0	0.0
44	0.9089594853446095	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTCGA	20	4.0792412E-4	50.171978	37
TAACGGC	20	4.0792412E-4	50.171978	37
AGAGGAG	40	2.5247573E-9	50.171978	38
GAGCAGG	20	4.0792412E-4	50.171978	50
CGGCGAG	20	4.0792412E-4	50.171978	40
AGCGGGA	20	4.0792412E-4	50.171978	44
CCTTCTC	40	2.5247573E-9	50.171978	50
CCCGAAT	20	4.0792412E-4	50.171978	40
GGTAGGC	20	4.0792412E-4	50.171978	48
CGAAAAG	20	4.0792412E-4	50.171978	39
GAGAGGA	40	2.5247573E-9	50.171978	37
CTTGAAA	20	4.0792412E-4	50.171978	46
AACGGCG	20	4.0792412E-4	50.171978	38
GTAACGG	20	4.0792412E-4	50.171978	36
CCGAATG	20	4.0792412E-4	50.171978	41
GTGCTCG	20	4.0792412E-4	50.171978	36
CGCTGGC	15	0.008275543	50.171974	50
AGCTCAA	35	5.02896E-8	50.171974	37
CAGCACT	15	0.008275543	50.171974	42
ACTGACG	25	2.0199428E-5	50.171974	42
>>END_MODULE