SRR212518_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212518_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47097
Sequences flagged as poor quality	0
Sequence length	56
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	2989	6.3464764210034605	No Hit
CAAAGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1408	2.9895747075185257	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1075	2.282523302970465	No Hit
CAAAGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	640	1.3588975943266026	No Hit
CAAAGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	548	1.1635560651421535	No Hit
CAAAGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	354	0.751640231861902	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	189	0.40129944582457483	Illumina Single End Adapter 2 (95% over 23bp)
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	182	0.3864365033866276	No Hit
CAAAGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	145	0.3078752362146209	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGT	145	0.3078752362146209	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	116	0.2463001889716967	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	112	0.23780707900715545	Illumina Single End Adapter 2 (96% over 30bp)
CAAAGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	112	0.23780707900715545	No Hit
CAAAGTGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	112	0.23780707900715545	No Hit
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT	107	0.22719069155147886	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.21869758158693758	No Hit
CAAAGTGGGCTGGGACATCTGTCTGGCATTTGCCTGTAGTGCGTTGTATTGCAGCT	98	0.208081194131261	No Hit
CAAAGTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96	0.2038346391489904	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96	0.2038346391489904	No Hit
CAAAGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.18684841921990786	No Hit
CAAAGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.17835530925536658	No Hit
CAAAGTGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA	69	0.14650614688833685	No Hit
CAAAGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCCGAAAAACATCG	68	0.14438286939720152	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAGCAGG	59	0.12527337197698366	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGGGGGAAGCAGG	58	0.12315009448584835	No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.12102681699471304	No Hit
CAAAGTGGGTAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT	57	0.12102681699471304	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	56	0.11890353950357772	Illumina Single End Adapter 2 (100% over 29bp)
CAAAGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGAT	56	0.11890353950357772	No Hit
CAAAGTGGGATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCA	55	0.11678026201244242	No Hit
CAAAGTGGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTGAAGCAGCA	54	0.11465698452130708	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT	53	0.11253370703017176	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	52	0.11041042953903646	No Hit
CAAAGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT	48	0.1019173195744952	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGCCG	20	4.0130963E-4	50.322235	49
GGCCTTC	80	0.0	50.322235	48
GTTGCAA	20	4.0130963E-4	50.322235	48
CGGGGAT	15	0.008170487	50.32223	50
ATGGCGG	15	0.008170487	50.32223	46
TTACACT	15	0.008170487	50.32223	48
CAATTGA	15	0.008170487	50.32223	46
CTTGGTA	15	0.008170487	50.32223	48
CACTTGG	15	0.008170487	50.32223	46
GGCGGAA	15	0.008170487	50.32223	48
ACTGACG	20	4.0344556E-4	50.26824	42
AACTGAC	20	4.0344556E-4	50.26824	41
CTGACGG	20	4.0344556E-4	50.26824	43
AGAGCTG	20	4.0344556E-4	50.26824	42
CCAAGTG	20	4.0344556E-4	50.26824	41
CTCGTTG	20	4.0344556E-4	50.26824	45
TGACGGC	20	4.0344556E-4	50.26824	44
TTACACG	15	0.008205285	50.268238	44
GCGCGGG	15	0.008205285	50.268238	43
CCGTCTC	15	0.008205285	50.268238	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content