FastQCFastQC Report
Tue 31 May 2016
SRR212517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134650
Sequences flagged as poor quality0
Sequence length58
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT102867.63906424062384No Hit
GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG21051.5633122911251394No Hit
GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC20261.5046416635722242No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGTGG15371.1414779056813962No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14051.0434459710360193No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC8440.6268102487931675Illumina Single End Adapter 2 (96% over 25bp)
GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT8440.6268102487931675No Hit
GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC8250.6126995915336056No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGGGG6550.4864463423691051No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.43223171184552545No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT5240.3891570738952841Illumina Single End Adapter 2 (96% over 32bp)
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.31340512439658375No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC3990.29632380245079837No Hit
GGACATGGGGGGAATTCCGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3840.28518380987746006No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC3550.26364649090233944Illumina Single End Adapter 2 (100% over 31bp)
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.2584478277014482No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT3200.2376531748978834No Hit
GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2710.20126253249164502No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG2700.2005198663200891Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2690.19977720014853323No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.1960638692907538No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2620.19457853694764204No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT2610.19383587077608616No Hit
GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2590.1923505384329744No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGTGG2420.17972521351652432No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT2370.17601188265874487Illumina Single End Adapter 2 (100% over 24bp)
GGACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2280.16932788711474192No Hit
GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGAAC2150.1596732268845154No Hit
GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2130.15818789454140364Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA2100.155959896026736No Hit
GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCGAA2090.1552172298551801No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA2060.15298923134051245No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTTTT1700.12625324916450056No Hit
GGACATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT1670.1240252506498329No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.12105458596360935No Hit
GGACATGGGGGGAATTCCTTCAGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1590.11808392127738582No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.11511325659116227No Hit
GGACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1540.11437059041960639No Hit
GGACATGGGAAGCAGTGGTATCAACGCGAACGGAAGAGCTCGTATGCCGTCTTCTGCT1530.11362792424805052Illumina Single End Adapter 1 (96% over 31bp)
GGACATGGGGGGAATTCCGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1520.11288525807649462No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGT1490.11065725956182697Illumina Single End Adapter 2 (100% over 24bp)
GGACATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1480.10991459339027107No Hit
GGACATGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1470.10917192721871519No Hit
GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.10323059784626809No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGGGG1370.10174526550315634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG251.6348242E-552.06696752
CGCTCCG1050.052.06696752
AAGATGC1000.052.06696752
CGCCCCG150.007169767352.06696352
TCGAACT800.052.06696352
ATGAAGG203.4149474E-452.06696352
AGGCGAT150.007169767352.06696352
AAGAAGT353.810237E-852.06696350
CAAACAA150.007169767352.06696351
CTTCCGA203.4149474E-452.06696352
CGTTTGG150.007180325752.04760443
GGTTGCG251.6384301E-552.04760448
TTGCAAT251.6384301E-552.04760449
CCGTGGG251.6384301E-552.04760443
CATCATT150.007180325752.04760441
GCGTGTA150.007180325752.04760445
GGTTATG150.007180325752.04760441
CGGGTTG307.8920493E-752.04760446
AGGTGAG150.007180325752.04760449
CGGGTAA150.007180325752.04760449
Produced by FastQC (version 0.11.5)