FastQCFastQC Report
Tue 31 May 2016
SRR212516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133384
Sequences flagged as poor quality0
Sequence length58
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT102467.681580999220296No Hit
GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG21091.581149163317939No Hit
GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC19701.4769387632699573No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13751.0308582738559346No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGTGG12470.9348947399988005No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC8430.6320098362622204Illumina Single End Adapter 2 (96% over 25bp)
GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT8160.6117675283392311No Hit
GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC7940.5952737959575362No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT5780.433335332573622Illumina Single End Adapter 2 (96% over 32bp)
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.42283932105799793No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGGGG5020.37635698434594855No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC4200.3148803454687219Illumina Single End Adapter 2 (100% over 31bp)
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.2946380375457326No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC3470.260151142565825No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.24290769507587118No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT3130.23466082888502368No Hit
GGACATGGGGGGAATTCCGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3050.22866310801895282No Hit
GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2880.21591795117855214No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG2830.21216937563725782Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2790.2091705152042224No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT2630.19717507347208063No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.189677922389492No Hit
GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2530.189677922389492No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT2330.17468362022431477Illumina Single End Adapter 2 (100% over 24bp)
GGACATGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2270.1701853295747616No Hit
GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2250.16868589935824385Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGAAC2250.16868589935824385No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGTGG2160.1619384633839141No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA2150.16118874827565527No Hit
GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA2000.1499430216517723No Hit
GGACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1930.1446950158939603No Hit
GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCGAA1850.1386972950278894No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTTTT1630.12220356264619443No Hit
GGACATGGGGGGAATTCCTTCAGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1620.12145384753793559No Hit
GGACATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1620.12145384753793559No Hit
GGACATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT1580.11845498710490014No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGGGG1580.11845498710490014No Hit
GGACATGGGGGGAATTCCGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1550.11620584178012355No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.11545612667186468No Hit
GGACATGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1520.11395669645534696No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.11170755113057039No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGT1440.10795897558927607Illumina Single End Adapter 2 (100% over 24bp)
GGACATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1420.10645954537275835No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCT1370.10271096983146405Illumina Single End Adapter 2 (100% over 29bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT1360.10196125472320518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGAG900.052.1173427
CGCGATC850.052.1173425
TAAACGA150.00714223852.11733623
AGAAGGT150.00714223852.11733626
AACGACG150.00714223852.11733625
GGCGAGC150.00714223852.11733627
GCGTGTC150.00714223852.11733623
GTCTCCG150.00714223852.11733627
CAAAGAG150.00714223852.11733627
CGACGTA150.00714223852.11733627
CGTGTCT150.00714223852.11733624
ACGACGT150.00714223852.11733626
ACGAAGA150.00714223852.11733624
ACGCGAA307.818817E-752.11733624
CGCGATG150.00714223852.11733625
AACGCAC251.6254004E-552.11733223
ACGCACG251.6254004E-552.11733224
GCGAACG251.6254004E-552.11733226
ACGCGGT251.6254004E-552.11733224
ACGCGAT1000.052.11733224
Produced by FastQC (version 0.11.5)