##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 133384 Sequences flagged as poor quality 0 Sequence length 58 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.15654801175553 38.0 36.0 39.0 33.0 40.0 2 36.67995411743537 38.0 35.0 39.0 31.0 40.0 3 35.68033647214059 38.0 35.0 39.0 30.0 40.0 4 36.83700443831344 38.0 36.0 39.0 33.0 40.0 5 36.42220206321598 38.0 35.0 39.0 31.0 40.0 6 36.032275235410545 38.0 35.0 39.0 30.0 40.0 7 35.95023391111378 38.0 35.0 39.0 30.0 40.0 8 35.236939962814134 38.0 34.0 39.0 26.0 40.0 9 34.76791819108739 38.0 33.0 39.0 25.0 40.0 10 34.33961344689018 37.0 33.0 39.0 25.0 40.0 11 35.158962094404124 38.0 35.0 39.0 28.0 40.0 12 35.082236250224916 38.0 34.0 39.0 28.0 40.0 13 34.71544563066035 38.0 33.0 39.0 27.0 40.0 14 34.49798326635879 38.0 33.0 39.0 26.0 40.0 15 34.64042913692797 38.0 33.0 39.0 27.0 40.0 16 33.98917411383674 37.0 33.0 39.0 25.0 40.0 17 34.66503478678102 38.0 35.0 39.0 27.0 40.0 18 34.4857029328855 38.0 35.0 39.0 27.0 40.0 19 32.84359443411503 36.0 32.0 39.0 21.0 40.0 20 33.699994002279134 38.0 33.0 39.0 23.0 40.0 21 33.597035626461945 38.0 33.0 39.0 22.0 40.0 22 33.37338811251724 38.0 33.0 39.0 22.0 40.0 23 33.00569033767169 38.0 33.0 39.0 21.0 40.0 24 32.96420860073172 38.0 33.0 39.0 19.0 40.0 25 32.75518053139807 38.0 33.0 39.0 17.0 40.0 26 31.747428477178673 37.0 32.0 39.0 2.0 40.0 27 31.51230282492653 37.0 32.0 39.0 2.0 40.0 28 30.366640676542914 36.0 29.0 38.0 2.0 40.0 29 30.673416601691358 36.0 30.0 39.0 2.0 40.0 30 29.28825796197445 35.0 28.0 38.0 2.0 40.0 31 27.272041624182812 35.0 17.0 38.0 2.0 40.0 32 25.930224014874348 33.0 10.0 38.0 2.0 40.0 33 26.635585977328613 35.0 11.0 38.0 2.0 40.0 34 26.16528219276675 34.0 2.0 38.0 2.0 40.0 35 25.24574911533617 33.0 2.0 38.0 2.0 40.0 36 24.456104180411444 34.0 2.0 39.0 2.0 40.0 37 24.186214238589336 33.0 2.0 39.0 2.0 40.0 38 24.132129790679542 33.0 2.0 39.0 2.0 40.0 39 24.066567204462306 33.0 2.0 39.0 2.0 40.0 40 23.95681640976429 33.0 2.0 39.0 2.0 40.0 41 24.26430456426558 35.0 2.0 39.0 2.0 39.0 42 24.067316919570562 34.0 2.0 39.0 2.0 40.0 43 23.96156960355065 34.0 2.0 39.0 2.0 39.0 44 23.82035326575901 33.0 2.0 39.0 2.0 39.0 45 23.779126431955856 34.0 2.0 39.0 2.0 39.0 46 23.744512085407546 34.0 2.0 39.0 2.0 40.0 47 23.601728843039645 34.0 2.0 39.0 2.0 40.0 48 23.403766568703894 34.0 2.0 39.0 2.0 40.0 49 23.23459335452528 33.0 2.0 39.0 2.0 40.0 50 23.11098782462664 33.0 2.0 39.0 2.0 39.0 51 22.7260166136868 33.0 2.0 39.0 2.0 39.0 52 22.512872608408806 33.0 2.0 39.0 2.0 39.0 53 22.283924608648714 32.0 2.0 39.0 2.0 39.0 54 22.041661668565943 32.0 2.0 39.0 2.0 39.0 55 21.733971090985424 32.0 2.0 39.0 2.0 39.0 56 21.243717387392792 31.0 2.0 38.0 2.0 39.0 57 21.075286391171353 31.0 2.0 38.0 2.0 39.0 58 20.87746656270617 30.0 2.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.011003220167602 1 2 -2.2462171150392862 1 3 -2.432155643173452 1 4 -1.9191642112294005 1 5 -2.4258098164317374 1 6 -2.857417263252991 1 7 -3.2043383978815285 1 8 -3.034233665709259 1 9 -2.8004116599088604 1 10 -3.3176075381770147 1 11 -2.3809207920408255 1 12 -2.4810646404095635 1 13 -2.131548944293492 1 14 -2.6265418821906543 1 15 -2.808584446616205 1 16 -1.1615952535059009 1 17 -1.2727654668252697 1 18 -0.9954635504772895 1 19 -1.9858004767686204 1 20 -1.2217230463031257 1 21 -1.4248277771302753 1 22 -1.7071113026357025 1 23 -1.4069302402874975 1 24 -1.5180530647047057 1 25 -1.6112663413914845 1 26 -2.4109276861424007 1 27 -2.5330726485247688 1 28 -2.712098573397668 1 29 -3.2818265107858586 1 30 -2.7103254850921594 1 31 -2.6495982631386177 1 32 -3.0793425034001025 1 33 -2.201513661218815 1 34 -2.875694434293006 1 35 -3.597041745346143 1 36 -2.014067242566732 1 37 -2.324752914617619 1 38 -2.3103399706597365 1 39 -2.299615572238814 1 40 -2.313830137399563 1 41 -2.063992948383376 1 42 -2.842065338696081 1 43 -2.7688423753252174 1 44 -2.3882331358921007 1 45 -2.599709324335411 1 46 -1.762879749091038 1 47 -1.8999943934820642 1 48 -2.101235109782671 1 49 -2.0770593333535885 1 50 -1.3886249190393052 1 51 -1.2123514540469778 1 52 -1.194363340252746 1 53 -1.452226823928111 1 54 -1.949716765320396 1 55 -1.3687635502649833 1 56 -1.5974437577136094 1 57 -2.4642678478530406 1 58 -2.0031859253994533 2 1 -0.2141081909694833 2 2 -0.10699193344122193 2 3 0.04448586088579276 2 4 -0.14520038841214244 2 5 -0.06446527861660911 2 6 -0.2030945172054217 2 7 -0.21003559201341915 2 8 -0.5062461995363492 2 9 -1.6318459285315612 2 10 -0.023133816484950387 2 11 -0.37864191438806927 2 12 -1.19150474865625 2 13 -1.1363203443789516 2 14 -0.4315554130267216 2 15 -0.8067328585233398 2 16 -1.1432218024305527 2 17 -0.9231001769805189 2 18 -0.23431841445678003 2 19 -0.7742209296955522 2 20 0.20307399115592517 2 21 -0.9890636409673306 2 22 -0.6870999082474398 2 23 -0.5796264374104112 2 24 -0.6137659261204576 2 25 -0.9145707139879811 2 26 -0.32069837407859225 2 27 0.1312914021802598 2 28 -0.41392167551987313 2 29 -0.3514747090482153 2 30 -0.7056252999333523 2 31 -1.5455532553049736 2 32 -1.531699717472172 2 33 -1.8178788513626678 2 34 -1.1639012424399944 2 35 -1.266889345403115 2 36 -0.9093813003932496 2 37 -0.7361615458667288 2 38 -0.5281152163512317 2 39 -0.15590385026188613 2 40 0.04110505701718736 2 41 0.5129903873237893 2 42 0.2218856654343817 2 43 0.8921033589006768 2 44 0.2121086957558127 2 45 0.3557101315825477 2 46 0.46308521316505136 2 47 0.3283918762800795 2 48 0.592469490701589 2 49 0.3908939496545294 2 50 0.502985962601489 2 51 0.8571543143620808 2 52 0.8600448637068041 2 53 1.3962990270902615 2 54 1.1402989019634653 2 55 0.8113389992293918 2 56 0.9583887447789117 2 57 1.5793156872559173 2 58 1.6683708328970859 3 1 -0.2764947455913287 3 2 -0.20642050486979002 3 3 -0.6718166601226088 3 4 -0.4734692959751641 3 5 -0.4338098164317401 3 6 -1.4010104835919748 3 7 -1.490304499576439 3 8 -1.7797251911329894 3 9 -2.1650896260105554 3 10 -2.0272346568210864 3 11 -0.7017343513628589 3 12 -1.0634375217654934 3 13 -1.171345554462988 3 14 -1.495152051682183 3 15 -1.4922454635653608 3 16 -1.8951545755397987 3 17 -1.9745959752998417 3 18 -1.7471923640366143 3 19 -2.439834375073705 3 20 -1.9588755886760048 3 21 -2.456895573740443 3 22 -2.909755370432311 3 23 -3.0297776979146143 3 24 -2.0998835731792838 3 25 -2.7444866803745356 3 26 -4.161639550549179 3 27 -2.9777506146264656 3 28 -3.573047725940043 3 29 -3.585962104006196 3 30 -4.047003451193856 3 31 -3.740242330935228 3 32 -4.061850977976373 3 33 -4.132971288337458 3 34 -3.884304603784532 3 35 -3.4849061521258022 3 36 -3.924440123922661 3 37 -3.4743800332616885 3 38 -3.3055942079478697 3 39 -3.261920656984575 3 40 -3.0685420018063425 3 41 -3.466975999230833 3 42 -3.1998958471706587 3 43 -2.924300002443861 3 44 -3.3075551697904046 3 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0.3553092144333654 4 28 -0.8615263584186756 4 29 -0.03150056554465763 4 30 0.30198800179759644 4 31 -0.96055002324292 4 32 -1.1491672172926108 4 33 -0.8224755618417312 4 34 -1.1466635781435066 4 35 -0.7106155538352041 4 36 -0.6077221752047137 4 37 -0.4447731956548502 4 38 -0.9948249771786415 4 39 -0.7791172381811577 4 40 -1.4355163607806993 4 41 -1.1465657428205773 4 42 -1.368647983922795 4 43 -1.6889495750934351 4 44 -1.3321705544057885 4 45 -1.357785523089575 4 46 -1.5140748816421024 4 47 -1.2092077789666327 4 48 -1.2170543188222211 4 49 -0.8777981425669736 4 50 -1.1074428242978875 4 51 -1.231763303967302 4 52 -1.343718692997932 4 53 -1.8280779031690209 4 54 -2.0327241534125307 4 55 -1.9878422117165258 4 56 -1.273888781616261 4 57 -1.4487673408271107 4 58 -1.0708376710173013 5 1 -0.12216141225800214 5 2 -0.15342050486979275 5 3 -0.4451499934559422 5 4 0.007530704024837576 5 5 -0.6341431497650731 5 6 -0.7430104835919735 5 7 -1.1573044995764405 5 8 -1.3120585244663232 5 9 -1.7160896260105574 5 10 -0.8085679901544154 5 11 -0.034734351362857296 5 12 -0.07343752176549145 5 13 0.21732111220367756 5 14 -0.18148538501551315 5 15 -0.31324546356535876 5 16 0.11651209112687155 5 17 0.3124040247001574 5 18 0.6391409692967187 5 19 0.18383229159296377 5 20 0.5457910779906641 5 21 0.8227710929262244 5 22 1.0409112962343556 5 23 0.5588889687520506 5 24 0.6504497601540464 5 25 0.17051331962546357 5 26 0.0680271161174879 5 27 0.10958271870686787 5 28 -0.5207143926067097 5 29 -0.13662877067286416 5 30 -1.5926701178605214 5 31 -0.9515756642685602 5 32 -1.9765176446430388 5 33 -1.5406379550041258 5 34 -3.097304603784533 5 35 -3.3589061521258046 5 36 -3.031440123922664 5 37 -3.4157133665950212 5 38 -3.3589275412812043 5 39 -3.314587323651242 5 40 -3.7015420018063416 5 41 -3.3029759992308314 5 42 -3.6652291805039923 5 43 -3.1829666691105274 5 44 -3.1392218364570716 5 45 -3.0464607367647893 5 46 -2.406382573949795 5 47 -2.405361625120481 5 48 -2.3837209854888854 5 49 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5.767618771415073E-4 7 15 -0.31705902288739196 7 16 -0.2463410162177624 7 17 0.057743007751000164 7 18 0.17402797494644062 7 19 -1.2908852225313296 7 20 -0.7132484700319353 7 21 -0.4841498110285798 7 22 -0.9359248619577372 7 23 -0.5848963419824145 7 24 -0.3994089969081003 7 25 -0.5095714261372493 7 26 -0.029571753939009682 7 27 -0.27036078411799025 7 28 -2.341666180072366E-4 7 29 -0.5106400701078933 7 30 -1.439139044414194 7 31 -3.047903347884379 7 32 -2.672562842383151 7 33 -3.1506662035916975 7 34 -2.384169010564193 7 35 -2.469923101278347 7 36 -2.5649147001938495 7 37 -2.5620410502108406 7 38 -2.8442382757444804 7 39 -3.121649470543897 7 40 -3.7121352221453243 7 41 -4.284331931434224 7 42 -4.214946694628285 7 43 -4.785791527867591 7 44 -4.167894152841253 7 45 -4.192929663318463 7 46 -4.389998393158834 7 47 -4.340672359583756 7 48 -4.491065618257249 7 49 -4.924516960472236 7 50 -5.024218139378288 7 51 -4.263198911674095 7 52 -4.143515882625628 7 53 -3.748836993419637 7 54 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-0.48382831308367713 118 14 -0.6041865344407995 118 15 -1.4503144290825958 118 16 -1.4665338858846226 118 17 -1.9988028718515665 118 18 -1.2985027088641985 118 19 -1.8681792026599098 118 20 -1.4208755886760045 118 21 -1.4395852289128541 118 22 -1.1016174393978275 118 23 -1.0267432151559959 118 24 -1.106159435248248 118 25 -1.0148315079607428 118 26 -0.31157058503193724 118 27 -0.051336821523015885 118 28 -0.6514615190434903 118 29 -0.6216862419372333 118 30 -1.6596241408490258 118 31 -1.7682423309352266 118 32 -1.8408854607349916 118 33 -1.5662126676478039 118 34 -1.6119597761983258 118 35 -1.3347682210913199 118 36 -2.3150608135778334 118 37 -1.5410007229168592 118 38 -2.3807666217409746 118 39 -1.5341275535362975 118 40 -1.8524040707718576 118 41 -1.5926311716446264 118 42 -2.0767234333775555 118 43 -1.2800931058921385 118 44 -1.3699689628938536 118 45 -1.4220354494084688 118 46 -2.2092561371681825 118 47 -1.8513386366147344 118 48 -2.007284203879692 118 49 -1.847515207111627 118 50 -1.9248610382678244 118 51 -1.6825443237138415 118 52 -2.2284077587214774 118 53 -2.0897195182589137 118 54 -1.9016454619890126 118 55 -2.8762153328482682 118 56 -2.2895828576551622 118 57 -1.9847237216110791 118 58 -2.3120696191458023 119 1 -0.10049474559133387 119 2 -0.5664205048697895 119 3 -0.3198166601226049 119 4 -0.497469295975165 119 5 -0.5778098164317385 119 6 -0.7610104835919742 119 7 -1.0743044995764421 119 8 -0.8837251911329886 119 9 -1.501089626010554 119 10 -0.6512346568210816 119 11 -0.589734351362857 119 12 -0.5194375217654965 119 13 -0.6913455544629841 119 14 -0.6231520516821831 119 15 -0.4682454635653599 119 16 -0.007154575539793484 119 17 0.13740402470015312 119 18 -0.05919236403661188 119 19 -0.17583437507370547 119 20 -0.022875588676001257 119 21 -0.6808955737404432 119 22 -0.17375537043231049 119 23 -0.20577769791461265 119 24 -0.3318835731792831 119 25 -0.06448668037453587 119 26 -0.049639550549180456 119 27 0.05424938537353441 119 28 0.5389522740599588 119 29 -0.08996210400619731 119 30 0.968996548806146 119 31 0.01175766906477449 119 32 0.9541490220236284 119 33 0.5630287116625396 119 34 0.5236953962154693 119 35 0.9710938478741973 119 36 2.427559876077339 119 37 2.109619966738311 119 38 2.230405792052128 119 39 2.1700793430154235 119 40 1.8594579981936583 119 41 2.453024000769169 119 42 1.9761041528293397 119 43 1.7716999975561407 119 44 2.228444830209593 119 45 2.3592059299018757 119 46 1.8549507593835415 119 47 2.1259717082128518 119 48 1.593612347844445 119 49 2.2893813446125115 119 50 2.4890699962149334 119 51 2.9228349866309884 119 52 2.992212930933693 119 53 2.3063494472583272 119 54 2.3621476414592664 119 55 2.495439839565524 119 56 2.09862403889656 119 57 2.01017283011306 119 58 1.8880683118886807 120 1 -1.0654504978037238 120 2 -1.5102789119494346 120 3 -1.5082768371137618 120 4 -1.684725933143305 120 5 -1.8063407898830661 120 6 -2.2053998641229455 120 7 -2.0681452075410434 120 8 -3.3453181114869714 120 9 -3.590647148134451 120 10 -2.425677134697189 120 11 -2.481415767292063 120 12 -2.8021985837123964 120 13 -3.035628740303693 120 14 -2.4320016092043026 120 15 -2.614794136131728 120 16 -2.240641301203514 120 17 -1.9900296036184315 120 18 -1.7112277622667023 120 19 -3.9317281803834376 120 20 -1.9363623143397213 120 21 -2.0290725648908854 120 22 -2.024870414680098 120 23 -1.4533529191535521 120 24 -1.1910694138872486 120 25 -1.7133362378966588 120 26 -1.655940435504931 120 27 -0.9958745084317755 120 28 -1.2998441861170313 120 29 -0.19828068807699495 120 30 -0.6642777874770402 120 31 -1.5956759592538106 120 32 -1.8744527478878759 120 33 -2.1153429697533888 120 34 -2.4200922144040007 120 35 -1.5389946477010241 120 36 -3.698033044276645 120 37 -3.1673357854740765 120 38 -3.322939340691235 120 39 -3.419159595037673 120 40 -3.4570729752576703 120 41 -3.588816707195434 120 42 -4.028356024161809 120 43 -3.505680533417312 120 44 -3.2838383556311115 120 45 -3.205679025850337 120 46 -2.2801288866341594 120 47 -2.5804353714331647 120 48 -2.576936324721924 120 49 -2.5056629031751 120 50 -1.6320627471478986 120 51 -2.711076517793792 120 52 -2.8348313168539185 120 53 -2.486500110263794 120 54 -2.5387373142929484 120 55 -3.361338921496422 120 56 -2.1524910053512265 120 57 -2.2955262849311886 120 58 -2.114480360677689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 469.0 3 122.0 4 446.0 5 247.0 6 648.0 7 1188.0 8 909.0 9 1413.0 10 1514.0 11 1350.0 12 1816.0 13 1990.0 14 1981.0 15 2263.0 16 2878.0 17 3254.0 18 3760.0 19 4331.0 20 4320.0 21 2693.0 22 3501.0 23 2474.0 24 2138.0 25 1685.0 26 1606.0 27 1805.0 28 2201.0 29 2620.0 30 3105.0 31 3928.0 32 4653.0 33 5406.0 34 5859.0 35 6753.0 36 8524.0 37 13067.0 38 15297.0 39 11128.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 31.207641096383377 57.81877886403167 5.197774845558688 5.77580519402627 11 23.04474299766089 57.75205421939663 12.622203562646195 6.580999220296288 12 70.05113057038326 14.517483356324595 8.92460864871349 6.50677742457866 13 15.489863851736342 19.811971450848677 13.54660229112937 51.15156240628561 14 15.564835362562226 62.58246866190847 13.081029208900619 8.771666766628682 15 56.63347927787441 15.501859293468481 19.372638397408984 8.492023031248126 16 13.14625442331914 14.773885923349129 58.52501049601152 13.554849157320218 17 53.146554309362436 14.517483356324595 11.626581898878426 20.709380435434536 18 53.08345767134277 16.399121261424725 13.71267048053204 16.804750586700457 19 15.079472184735343 15.976158344579398 58.15039736092368 10.793972109761583 20 16.76962754153422 53.5791399268278 15.901457446170456 13.749775085467522 21 16.93906315600072 17.93393510466023 50.74596653271757 14.381035206621483 22 19.54072108139718 20.43813679404422 10.520830990456055 49.500311134102546 23 18.743627421579802 57.793288550350866 10.93309542373898 12.529988604330356 24 12.526055334782935 61.219914523506034 14.512259128739597 11.741771012971434 25 13.49432457153139 21.371699329744644 16.929570706692058 48.20440539203191 26 51.93249210883436 19.95021630416039 13.689016846982613 14.428274740022642 27 15.240050536955149 17.432241374123876 15.195680293595645 52.13202779532533 28 16.10008097651681 54.78961101280628 16.692409201331614 12.41789880934529 29 48.47746376431244 20.366968349542226 21.07856002039546 10.07700786574987 30 14.869182993789194 54.27930630982027 22.52917879324312 8.32233190314741 31 46.48801847464236 22.580451669015986 23.19977206610083 7.73175779024083 32 17.547280171573405 23.003434467657513 49.967005114207296 9.482280246561784 33 35.76646715569357 19.33081319472571 22.57774177579767 22.32497787378306 34 25.09768334845769 20.840114295142456 24.28771777199469 29.774484584405165 35 17.939641207175857 48.93502129957401 23.121287574248512 10.00404991900162 36 31.80126926019834 25.53673503068129 21.977255337344157 20.684740371776215 37 14.874291200384027 26.078575500285016 46.3652455671897 12.68188773214125 38 27.83337334213527 22.132119066194562 36.798595691052036 13.235911900618136 39 17.68165960160558 23.154143376974154 33.94605544509885 25.21814157632142 40 18.42500075014253 23.422450265550452 45.118822576289496 13.033726408017523 41 27.027189244343074 22.81750795270392 39.1460596602845 11.009243142668506 42 30.23910091454037 22.874012498968423 36.67164324673084 10.215243339760374 43 17.55783598883788 30.545053560176434 38.92264530260749 12.974465148378192 44 15.99159758430549 27.87801492929217 39.48235117596309 16.648036310439252 45 17.75605584354206 32.43636581871104 37.905942190981314 11.90163614676559 46 16.760950137298742 26.1407799768918 43.821556652611676 13.276713233197784 47 24.842394404250847 24.871664014889976 36.39263306415298 13.893308516706194 48 26.282691081010096 25.604292521856593 34.895501106900305 13.217515290233012 49 28.92923621952684 25.198901164885314 34.23051519154557 11.641347424042273 50 16.863201351097768 30.48752111090261 40.084068305498214 12.565209232501406 51 16.575601555111906 33.27504165478317 36.532370645011184 13.616986145093742 52 22.512982919580946 26.84687659472278 38.789661693633114 11.850478792063159 53 14.367518480993658 27.71961424443694 40.300198881759165 17.61266839281024 54 13.610191503577084 32.78982651322358 37.607819291489314 15.992162691710018 55 23.537142170556606 25.00750953711213 37.217265927728214 14.238082364603047 56 18.319073980875693 28.909553891338476 40.27897753306943 12.492394594716401 57 24.09193330328977 26.183716388763706 36.72525161484152 12.999098693105001 58 24.65113484445646 24.69244288224956 36.178330554428975 14.478091718865006 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 335.5 10 671.0 11 634.0 12 597.0 13 547.5 14 498.0 15 450.5 16 579.5 17 756.0 18 900.5 19 1045.0 20 1084.5 21 1124.0 22 1274.5 23 1709.5 24 1994.0 25 2491.0 26 2988.0 27 3241.0 28 4033.5 29 4573.0 30 5055.0 31 5537.0 32 4946.5 33 4356.0 34 9517.0 35 9334.0 36 3990.0 37 3338.5 38 2687.0 39 2566.5 40 2446.0 41 2384.0 42 2456.0 43 2590.0 44 3346.0 45 4102.0 46 4801.5 47 4578.0 48 3655.0 49 3831.0 50 4007.0 51 4261.0 52 4515.0 53 6564.0 54 8435.5 55 8258.0 56 8849.0 57 9440.0 58 8473.5 59 6557.5 60 5608.0 61 4931.0 62 4254.0 63 3639.0 64 3024.0 65 2788.0 66 2287.5 67 2023.0 68 1626.5 69 1230.0 70 890.5 71 551.0 72 378.0 73 138.5 74 72.0 75 55.5 76 39.0 77 31.5 78 17.5 79 11.0 80 7.5 81 4.0 82 4.5 83 5.0 84 3.5 85 3.5 86 5.0 87 4.0 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.008246866190847477 19 0.0029988604330354464 20 0.0 21 0.0 22 7.497151082588616E-4 23 0.0 24 0.010496011515624064 25 0.0014994302165177232 26 0.005248005757812032 27 0.308882624602651 28 0.00899658129910634 29 0.015744017273436096 30 0.05098062736160259 31 0.00899658129910634 32 0.022491453247765848 33 0.04348347627901397 34 0.03373717987164877 35 0.03598632519642536 36 0.05847777844419121 37 0.044982906495531695 38 0.05997720866070893 39 0.0742217957176273 40 0.05697834822767348 41 0.07197265039285072 42 0.06972350506807413 43 0.05697834822767348 44 0.06672464463503867 45 0.06222635398548551 46 0.07347208060936844 47 0.10496011515624064 48 0.09671324896539316 49 0.11395669645534696 50 0.11920470221315901 51 0.1094584058057938 52 0.09896239429016973 53 0.10421040004798177 54 0.13120014394530077 55 0.16493732381694956 56 0.19117735260600974 57 0.18293048641516224 58 0.17843219576560906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 133384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.10085167636298 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8149621965778 38.06453547651893 2 9.537604456824512 8.984585857374197 3 3.6657381615598887 5.179781682960475 4 1.867091126144051 3.5176632879505787 5 0.9884600079586152 2.3278654111437653 6 0.6796657381615598 1.9207701073592036 7 0.38519697572622363 1.2700173933905117 8 0.3326701153999204 1.2535236610088165 9 0.23716673298846003 1.0053679601751333 >10 1.3004377238360525 10.59722305523901 >50 0.0939116593712694 3.0056078690097765 >100 0.07958615200955034 7.096053499670125 >500 0.00955033824114604 3.0715827985365562 >1k 0.006366892160764028 5.023840940442632 >5k 0.0 0.0 >10k+ 0.001591723040191007 7.681580999220296 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 10246 7.681580999220296 No Hit GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 2109 1.581149163317939 No Hit GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1970 1.4769387632699573 No Hit GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1375 1.0308582738559346 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGTGG 1247 0.9348947399988005 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 843 0.6320098362622204 Illumina Single End Adapter 2 (96% over 25bp) GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 816 0.6117675283392311 No Hit GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 794 0.5952737959575362 No Hit GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 578 0.433335332573622 Illumina Single End Adapter 2 (96% over 32bp) GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 564 0.42283932105799793 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGGGG 502 0.37635698434594855 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC 420 0.3148803454687219 Illumina Single End Adapter 2 (100% over 31bp) GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 393 0.2946380375457326 No Hit GGACATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC 347 0.260151142565825 No Hit GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 324 0.24290769507587118 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT 313 0.23466082888502368 No Hit GGACATGGGGGGAATTCCGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG 305 0.22866310801895282 No Hit GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 288 0.21591795117855214 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG 283 0.21216937563725782 Illumina Single End Adapter 2 (96% over 30bp) GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC 279 0.2091705152042224 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT 263 0.19717507347208063 No Hit GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 253 0.189677922389492 No Hit GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 253 0.189677922389492 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT 233 0.17468362022431477 Illumina Single End Adapter 2 (100% over 24bp) GGACATGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG 227 0.1701853295747616 No Hit GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA 225 0.16868589935824385 Illumina Single End Adapter 2 (97% over 34bp) GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGAAC 225 0.16868589935824385 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGTGG 216 0.1619384633839141 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA 215 0.16118874827565527 No Hit GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 200 0.1499430216517723 No Hit GGACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 193 0.1446950158939603 No Hit GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCGAA 185 0.1386972950278894 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTTTT 163 0.12220356264619443 No Hit GGACATGGGGGGAATTCCTTCAGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 162 0.12145384753793559 No Hit GGACATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 162 0.12145384753793559 No Hit GGACATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 158 0.11845498710490014 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGGGG 158 0.11845498710490014 No Hit GGACATGGGGGGAATTCCGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG 155 0.11620584178012355 No Hit GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 154 0.11545612667186468 No Hit GGACATGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 152 0.11395669645534696 No Hit GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.11170755113057039 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTATGCCGT 144 0.10795897558927607 Illumina Single End Adapter 2 (100% over 24bp) GGACATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG 142 0.10645954537275835 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCT 137 0.10271096983146405 Illumina Single End Adapter 2 (100% over 29bp) GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT 136 0.10196125472320518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.08021951658369819 0.0 0.0 0.0 0.0 11 0.17468362022431477 0.0 0.0 0.0 0.0 12 0.17918191087386792 0.0 0.0 0.0 0.0 13 0.17993162598212678 0.0 0.0 0.0 0.0 14 0.26240028789060155 0.0 0.0 0.0 0.0 15 0.26539914832363704 0.0 0.0 0.0 0.0 16 0.2721465842979668 0.0 0.0 0.0 0.0 17 0.2751454447310022 0.0 0.0 0.0 0.0 18 0.27964373538055537 0.0 0.0 0.0 0.0 19 0.2863911713548851 0.0 0.0 0.0 0.0 20 0.3658609728303245 0.0 0.0 0.0 0.0 21 0.3786061296707251 0.0 0.0 0.0 0.0 22 0.4078450188928207 0.0 0.0 0.0 0.0 23 0.45207821028009354 0.0 0.0 0.0 0.0 24 0.5277994362142386 0.0 0.0 0.0 0.0 25 0.5382954477298626 0.0 0.0 0.0 0.0 26 0.5487914592454867 0.0 0.0 0.0 0.0 27 0.5577880405445931 0.0 0.0 0.0 0.0 28 0.9641336292208961 0.0 0.0 0.0 0.0 29 0.9821267918191088 0.0 0.0 0.0 0.0 30 1.1500629760690937 0.0 0.0 0.0 0.0 31 1.1628081329094944 0.0 0.0 0.0 0.0 32 1.1688058537755652 0.0 0.0 0.0 0.0 33 1.1793018652911893 0.0 0.0 0.0 0.0 34 1.1897978768068134 0.0 0.0 0.0 0.0 35 1.626132069813471 0.0 0.0 0.0 0.0 36 1.6516223834942723 0.0 0.0 0.0 0.0 37 1.6666166856594493 0.0 0.0 0.0 0.0 38 1.6763629820668147 0.0 0.0 0.0 0.0 39 1.6853595633659209 0.0 0.0 0.0 0.0 40 1.7003538655310981 0.0 0.0 0.0 0.0 41 1.7138487374797577 0.0 0.0 0.0 0.0 42 1.728093324536676 0.0 0.0 0.0 0.0 43 1.7378396209440412 0.0 0.0 0.0 0.0 44 1.7520842080009595 0.0 0.0 0.0 0.0 45 1.7640796497331015 0.0 0.0 0.0 0.0 46 1.7753253763569843 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGAGAG 90 0.0 52.11734 27 CGCGATC 85 0.0 52.11734 25 TAAACGA 15 0.007142238 52.117336 23 AGAAGGT 15 0.007142238 52.117336 26 AACGACG 15 0.007142238 52.117336 25 GGCGAGC 15 0.007142238 52.117336 27 GCGTGTC 15 0.007142238 52.117336 23 GTCTCCG 15 0.007142238 52.117336 27 CAAAGAG 15 0.007142238 52.117336 27 CGACGTA 15 0.007142238 52.117336 27 CGTGTCT 15 0.007142238 52.117336 24 ACGACGT 15 0.007142238 52.117336 26 ACGAAGA 15 0.007142238 52.117336 24 ACGCGAA 30 7.818817E-7 52.117336 24 CGCGATG 15 0.007142238 52.117336 25 AACGCAC 25 1.6254004E-5 52.117332 23 ACGCACG 25 1.6254004E-5 52.117332 24 GCGAACG 25 1.6254004E-5 52.117332 26 ACGCGGT 25 1.6254004E-5 52.117332 24 ACGCGAT 100 0.0 52.117332 24 >>END_MODULE