FastQCFastQC Report
Tue 31 May 2016
SRR212515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138458
Sequences flagged as poor quality0
Sequence length58
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT92326.667725952996577No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGTGG19621.4170362131476693No Hit
GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG17831.2877551315200277No Hit
GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC17281.2480318941484059No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12140.8768001848936139No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC7870.5684034147539326Illumina Single End Adapter 2 (96% over 25bp)
GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7620.5503473977668318No Hit
GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC7150.5164020858310824No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGGGG5400.3900099669213769No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4850.35028672954975515No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC4200.30334108538329313No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT3880.2802293836398041Illumina Single End Adapter 2 (96% over 32bp)
GGACATGGGGGGAATTCCGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG3830.27661818024238394No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT3420.2470063123835387No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.23400598015282612No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.2152277224862413No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTTTT2900.20944979705036906No Hit
GGACATGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2830.20439411229398086No Hit
GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2710.1957272241401725No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC2640.19067153938378426Illumina Single End Adapter 2 (100% over 31bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGT2450.17694896647358765Illumina Single End Adapter 2 (100% over 24bp)
GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG2280.16467087492235913Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2210.1596151901659709No Hit
GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2200.15889294948648686No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.15600398676855073No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2140.1545595054095827No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTTTT1790.1292810816276416No Hit
GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1780.12855884094815756No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA1780.12855884094815756No Hit
GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGAAC1740.12566987823022144No Hit
GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCGAA1710.12350315619176935No Hit
GGACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1690.12205867483280129No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGTGG1640.11844747143538112No Hit
GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1560.11266954599950887Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1440.10400265784570051No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCCACTTTTTTTTTTTTTTTTTTTTTT1390.10039145444828035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAT150.007042619452.3036848
GACAGTA150.007042619452.3036849
GGCTCTC150.007042619452.3036849
GATTCAC150.007042619452.3036849
GGTGCGT150.007042619452.3036849
GGCGTGA251.5916934E-552.303689
GGGATTC251.5916934E-552.303687
GAACAGT401.7807906E-952.303689
AAGTGTA203.3395717E-452.3036810
CTCGAAT150.007155018452.09477239
GCGGGCC307.8442827E-752.09477238
GCGGGAC401.8444553E-952.09477238
GTAGAGC203.4062532E-452.09477239
CTGGCTT150.007155018452.09477239
CGGGCTC203.4062532E-452.09477239
AGTACGT150.007155018452.09477238
CTGGCCT307.8442827E-752.09477239
GCCGGAC150.007155018452.09477238
GTACGTA150.007155018452.09477239
AGCTCGA150.007175597452.0569737
Produced by FastQC (version 0.11.5)