FastQCFastQC Report
Tue 31 May 2016
SRR212514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133762
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT112878.438121439571777No Hit
GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA17281.2918467128182893No Hit
GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA14951.1176567336014713No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA14311.0698105590526457No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGT13981.0451398753009076No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGG9240.6907791450486686No Hit
GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6830.5106083940132474No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG6760.5053752186719696Illumina Single End Adapter 2 (95% over 23bp)
GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT6470.4836949208295331No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5050.37753622104932644No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG4680.3498751513882866Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.273620310701096No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.24147366217610383No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT3080.23025971501622286Illumina Single End Adapter 2 (100% over 29bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT2940.21979336433366725No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA2770.2070842242191355No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC2200.16447122501158776Illumina Single End Adapter 2 (96% over 28bp)
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.15475247080635757No Hit
GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2060.15400487432903218No Hit
GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2020.15101448841973056No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT1970.14727650603310358No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC1840.1375577518278734Illumina Single End Adapter 2 (100% over 22bp)
GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGA1790.13381976944124638No Hit
GGACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.12335341875869081No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTT1650.12335341875869081No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGGAGCAGT1600.1196154363720638No Hit
GGACATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT1580.11812024341741302No Hit
GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC1570.11737264694008762No Hit
GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.11737264694008762No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.11438226103078603No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGG1530.11438226103078603No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1520.11363466455346063No Hit
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10840148921218283No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGGAGCAGG1440.10765389273485744No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.10541110330288123No Hit
GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCG1370.10242071739357964No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTGGACATGGGAAGCAGT1350.10092552443892884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAGT150.00824398450.25990750
ATGAAAG150.00824398450.25990748
GAGTAGG150.00824398450.25990749
ATCATCG150.00824398450.25990749
GCCGCTC700.050.25990750
TCATCGA150.00824398450.25990750
GGAGTAG150.00824398450.25990748
GGTCGTC204.065082E-450.25990348
CGTTTGG150.00825628850.2409543
AGGGGTC150.00825628850.2409545
AAATCAT150.00825628850.2409547
TGACAAG150.00825628850.2409541
TGGAGTA150.00825628850.2409547
GCGTGGG150.00825628850.2409543
ACCTGGG355.04715E-850.2409543
ACGAGGG150.00825628850.2409543
GACAAGG650.050.2409542
TCGCATG150.00825628850.2409541
GGGCGCT150.00825628850.2409541
GGACGAG150.00825628850.2409541
Produced by FastQC (version 0.11.5)