SRR212513_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212513_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113610
Sequences flagged as poor quality	0
Sequence length	56
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	9569	8.42267406038201	No Hit
GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1687	1.4849044978434998	No Hit
GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1502	1.3220667194789193	No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1332	1.1724320042249803	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGT	850	0.7481735762696945	No Hit
GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	659	0.5800545726608574	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGG	624	0.5492474254026933	No Hit
GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	606	0.5334037496699234	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	578	0.5087580318633923	Illumina Single End Adapter 2 (95% over 23bp)
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	475	0.41809699850365284	Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450	0.39609189331925004	No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.28958718422674057	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	306	0.26934248745709005	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	291	0.2561394243464484	Illumina Single End Adapter 2 (100% over 29bp)
GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	223	0.1962855382448728	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	217	0.19100431300061613	No Hit
GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	216	0.19012410879324004	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT	189	0.16635859519408502	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC	184	0.16195757415720446	Illumina Single End Adapter 2 (96% over 28bp)
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	184	0.16195757415720446	No Hit
GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC	159	0.1399524689728017	No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	156	0.13731185635067333	No Hit
GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	153	0.13467124372854503	Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCG	153	0.13467124372854503	No Hit
GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGA	151	0.1329108353137928	No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149	0.13115042689904058	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC	147	0.12939001848428835	Illumina Single End Adapter 2 (100% over 22bp)
GGACATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT	141	0.12410879324003168	No Hit
GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	134	0.1179473637883989	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT	134	0.1179473637883989	No Hit
GGACATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC	132	0.11618695537364669	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGG	131	0.11530675116627057	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTT	129	0.11354634275151836	No Hit
GGACATGGGTAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT	125	0.11002552592201391	No Hit
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.10826511750726169	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	122	0.10738491329988559	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGT	122	0.10738491329988559	No Hit
GGACATGGGAAGCAGTGGTATCAACGCGAACGGAAGAGCTCGTATGCCGTCTTCTG	115	0.10122348384825279	Illumina Single End Adapter 1 (96% over 29bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCTC	50	7.2759576E-12	50.305916	50
CATCGAA	50	7.2759576E-12	50.305916	50
GCGTGGA	15	0.008211003	50.305912	50
AGGCCAC	15	0.008211003	50.305912	48
GGGTTGC	15	0.008225452	50.283554	47
CGGGTGG	20	4.0530166E-4	50.283554	46
ATCAGAG	15	0.008225452	50.283554	47
CTGGTAG	25	2.0071024E-5	50.283554	46
CTCGTAG	20	4.0530166E-4	50.283554	46
CGTGGGA	35	5.022048E-8	50.26122	44
CGCGGGA	45	1.2732926E-10	50.26122	44
CAAGGGA	55	0.0	50.26122	44
ACAAAGG	15	0.00823992	50.261215	43
TGGCAAT	15	0.00823992	50.261215	42
GTCATGG	25	2.0123905E-5	50.261215	42
TAAGCCG	15	0.00823992	50.261215	43
TATGCGG	25	2.0123905E-5	50.261215	43
CGAAGGC	15	0.00823992	50.261215	43
GCGTGGG	25	2.0123905E-5	50.261215	43
CGTGGGG	15	0.00823992	50.261215	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content