##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212513_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113610 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.44794472317578 39.0 37.0 39.0 33.0 40.0 2 36.930824751342314 39.0 36.0 39.0 33.0 40.0 3 36.21723439838043 38.0 35.0 39.0 30.0 40.0 4 37.11943490889887 39.0 37.0 39.0 33.0 40.0 5 36.77945603379984 39.0 36.0 39.0 32.0 40.0 6 36.675829592465455 38.0 36.0 39.0 32.0 40.0 7 36.834301557961446 38.0 36.0 39.0 33.0 40.0 8 36.44796232725992 38.0 36.0 39.0 30.0 40.0 9 36.140163717982574 38.0 35.0 39.0 30.0 40.0 10 35.020165478390986 38.0 34.0 39.0 27.0 40.0 11 35.16993222427603 38.0 35.0 39.0 28.0 40.0 12 35.22610685679078 38.0 35.0 39.0 28.0 40.0 13 35.04098230789543 38.0 35.0 39.0 28.0 40.0 14 34.53007657776604 38.0 34.0 39.0 26.0 40.0 15 34.6260628465804 38.0 34.0 39.0 27.0 40.0 16 33.844379896135905 38.0 34.0 39.0 23.0 40.0 17 34.37949124196814 38.0 35.0 39.0 25.0 40.0 18 34.24670363524338 38.0 35.0 39.0 24.0 40.0 19 32.74910659272951 36.0 33.0 39.0 19.0 40.0 20 33.5538420913652 38.0 33.0 39.0 21.0 40.0 21 33.15907050435701 38.0 33.0 39.0 14.0 40.0 22 32.96048763313089 38.0 33.0 39.0 12.0 40.0 23 32.40344159845084 37.0 33.0 39.0 2.0 40.0 24 32.47811812340463 38.0 33.0 39.0 2.0 40.0 25 32.295863040225335 38.0 33.0 39.0 2.0 40.0 26 31.239133879059942 37.0 31.0 39.0 2.0 40.0 27 31.289384737259045 38.0 32.0 39.0 2.0 40.0 28 29.817234398380425 36.0 29.0 39.0 2.0 40.0 29 30.086251210280786 36.0 30.0 39.0 2.0 40.0 30 28.619267670099465 35.0 27.0 38.0 2.0 40.0 31 26.277352345744212 35.0 2.0 39.0 2.0 40.0 32 24.99426106856791 33.0 2.0 39.0 2.0 40.0 33 25.66646421969897 34.0 2.0 39.0 2.0 40.0 34 25.460197165742454 34.0 2.0 39.0 2.0 40.0 35 24.501531555320835 33.0 2.0 39.0 2.0 40.0 36 23.69070504357011 33.0 2.0 39.0 2.0 40.0 37 23.308036264413342 33.0 2.0 39.0 2.0 40.0 38 23.291567643693337 33.0 2.0 39.0 2.0 40.0 39 23.18483408150691 33.0 2.0 39.0 2.0 40.0 40 22.93826247689464 33.0 2.0 39.0 2.0 40.0 41 22.854810315993312 33.0 2.0 39.0 2.0 40.0 42 22.72503300765778 33.0 2.0 39.0 2.0 40.0 43 22.582906434292756 32.0 2.0 39.0 2.0 40.0 44 22.325684358771234 32.0 2.0 39.0 2.0 40.0 45 22.256834785670275 32.0 2.0 39.0 2.0 40.0 46 22.11027198310008 32.0 2.0 39.0 2.0 40.0 47 21.967529266789896 32.0 2.0 39.0 2.0 40.0 48 21.773822726872634 32.0 2.0 39.0 2.0 40.0 49 21.603520816829505 31.0 2.0 39.0 2.0 40.0 50 21.37041633659009 31.0 2.0 39.0 2.0 40.0 51 20.840832673180177 31.0 2.0 38.0 2.0 39.0 52 20.575116627057476 30.0 2.0 38.0 2.0 39.0 53 20.264677405157997 29.0 2.0 38.0 2.0 39.0 54 20.028272159140922 29.0 2.0 38.0 2.0 39.0 55 19.66939529970953 27.0 2.0 38.0 2.0 39.0 56 11.106355074377255 2.0 2.0 21.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.1552541400164955 1 2 -0.40560974230592706 1 3 -0.2857916892883736 1 4 -0.675076741298632 1 5 -0.6052907130011604 1 6 -0.3384671204800753 1 7 -0.4501036056843972 1 8 -0.7736524397081652 1 9 -0.4390201627923034 1 10 0.007125893548554529 1 11 0.16023465449467267 1 12 0.03211847996131212 1 13 0.2663560036354369 1 14 0.054166985185425176 1 15 0.3175338845158251 1 16 -0.001533599640652028 1 17 0.6291045868122964 1 18 0.7033915935908155 1 19 0.05221905610970623 1 20 0.9063173077410411 1 21 1.2706360619104586 1 22 0.20671750641268716 1 23 0.22619872860196466 1 24 0.3630848276250376 1 25 0.9745533431439242 1 26 0.49398054407322434 1 27 0.5538776130335101 1 28 0.7654236476889977 1 29 0.6260407405763715 1 30 -0.9034790530619965 1 31 -0.7494564470405258 1 32 -1.0548364862224062 1 33 -0.4212215805498367 1 34 -1.6782746067053687 1 35 -2.6222452778543506 1 36 -3.100004062215149 1 37 -3.1038683409300667 1 38 -2.9541182236840235 1 39 -3.473446819992173 1 40 -2.9217687226721942 1 41 -3.580856639150639 1 42 -3.470656671634373 1 43 -3.5143543798345043 1 44 -3.68230788413155 1 45 -3.6911564663452907 1 46 -3.1326420960846804 1 47 -3.7401919510222363 1 48 -3.7995568385293943 1 49 -4.82076429977268 1 50 -4.82314557826238 1 51 -5.059011418197416 1 52 -5.148528962933325 1 53 -4.923224835607883 1 54 -4.844928190001845 1 55 -4.792689745552893 1 56 -2.628264265789557 2 1 0.21940758908191782 2 2 0.2932223328970309 2 3 0.17909935187385884 2 4 -0.10457396391649354 2 5 0.21752654949705885 2 6 -0.3363306726806172 2 7 -0.09965495164650662 2 8 -0.03921290118088905 2 9 -0.051824606603723566 2 10 -0.37465732821472386 2 11 -0.2611440664852438 2 12 -0.4067791129741707 2 13 -0.17225672959344962 2 14 -0.20420076869579162 2 15 -0.07300877322524002 2 16 1.1908891321639388 2 17 0.6452703751615374 2 18 0.9732961655891046 2 19 0.7271229159587307 2 20 0.7816911861059452 2 21 0.24620934206998157 2 22 0.06193756053603039 2 23 -0.03352210888557039 2 24 0.07117484329232582 2 25 0.05937032078243476 2 26 -0.9601570431650579 2 27 -0.3396560716267949 2 28 -1.8171144522967566 2 29 -1.446954559238467 2 30 -1.9506233345033834 2 31 -2.35699098627995 2 32 -2.039952566552845 2 33 -1.58174002521584 2 34 -2.2497031781339416 2 35 -2.319510624671043 2 36 -2.147860492923023 2 37 -1.680210742297394 2 38 -1.6981717773088612 2 39 -1.7229839229689574 2 40 -1.779125224594999 2 41 -1.317290195624075 2 42 -1.5463581386618621 2 43 -1.5577954850901676 2 44 -1.814126713358153 2 45 -1.484490749210977 2 46 -1.906107414415402 2 47 -1.8926631089769437 2 48 -1.70028323078121 2 49 -1.2652878719134009 2 50 -1.2611173771514252 2 51 -1.3418771068457573 2 52 -1.3666602832580672 2 53 -0.8030852543516502 2 54 -0.9466943201827291 2 55 -0.35500280637043957 2 56 0.9254317889035057 3 1 0.34155837809419154 3 2 -0.040208409358953645 3 3 -0.04810696022433092 3 4 0.0745610448503129 3 5 -0.06841734296349244 3 6 0.2283975564251719 3 7 0.3435069419829446 3 8 0.4935164968319654 3 9 0.48397317247283667 3 10 -0.1148677314876636 3 11 0.7622340749525094 3 12 1.1065172049685543 3 13 1.1174861108507343 3 14 0.9639757362720616 3 15 1.2205330152025837 3 16 -0.483278021547342 3 17 -0.26121705908744275 3 18 -0.30914100565578195 3 19 -0.05195364745447506 3 20 -0.5343520634557137 3 21 0.015057968604168082 3 22 -1.2523523284177962 3 23 -0.5577769306272415 3 24 -0.5536059866316982 3 25 -0.292470707855788 3 26 -0.3919220928436111 3 27 -0.20742345911948945 3 28 0.022160825318671584 3 29 -0.7174104329965054 3 30 -0.18528142918485813 3 31 -1.8965877133401463 3 32 -1.664587283092878 3 33 -2.350734765134014 3 34 -2.551427316064977 3 35 -2.3535550431397745 3 36 -1.832255583513323 3 37 -1.871327048551045 3 38 -1.759247171814998 3 39 -1.6659997032144283 3 40 -1.451977357850403 3 41 -1.0782486994230247 3 42 -1.0982283899768817 3 43 -1.353821201045747 3 44 -1.1104446560817074 3 45 -1.4311593640560965 3 46 -1.3809759123698164 3 47 -1.3606642778840161 3 48 -1.4190149666082128 3 49 -1.2721552009607429 3 50 -1.3838526196996455 3 51 -1.4222394680380432 3 52 -1.796964199096756 3 53 -1.3410747341880054 3 54 -1.7072144838297199 3 55 -1.4397485690823046 3 56 -0.7402607885365882 4 1 -0.023232910472046342 4 2 -0.07832093204498136 4 3 -0.0727591078347345 4 4 -0.3452816653735269 4 5 -0.19772768779107963 4 6 -0.33618199590392805 4 7 0.11800784942396092 4 8 -0.016465354347708683 4 9 0.5034831543240159 4 10 -1.1266644647000064 4 11 -0.8890060944364819 4 12 -0.5551585361082729 4 13 -0.565060773601779 4 14 -0.11633279367348592 4 15 -0.37913425642476284 4 16 -0.3214510403011275 4 17 0.028559105461866352 4 18 0.6353538521784614 4 19 -0.8583662306365696 4 20 -0.3702564796686616 4 21 0.3810591785255255 4 22 0.5714528742440308 4 23 0.8118540433594461 4 24 0.24705947011361218 4 25 0.5538692800449994 4 26 0.6523912767873625 4 27 0.47330854330035166 4 28 -0.02139634346535857 4 29 0.8184334871486847 4 30 0.6683180868466003 4 31 0.8694437446150864 4 32 1.127457483997258 4 33 1.4410075216173475 4 34 0.373103839409918 4 35 0.576118278094345 4 36 1.1610898490335622 4 37 1.018570108133769 4 38 0.8880607531698779 4 39 0.7350892260051722 4 40 0.895421311236106 4 41 0.4674258317324522 4 42 0.4007129288373932 4 43 0.84853814559672 4 44 0.6968753681167179 4 45 0.9047450521568479 4 46 0.9081952915019329 4 47 1.3303520560542772 4 48 0.9422977980620821 4 49 1.6593330023060453 4 50 1.322438971346937 4 51 0.6437012458155564 4 52 0.5207913967895124 4 53 -0.18741472208878918 4 54 0.3827734169567272 4 55 1.095339150215942 4 56 0.026829350604367974 5 1 0.0632332549414869 5 2 0.5518482408873453 5 3 -0.10722026071694302 5 4 0.460952670466078 5 5 0.45405802649463567 5 6 0.4719320391837911 5 7 0.4558837892735852 5 8 0.5714105855019156 5 9 0.3540470640984523 5 10 -0.07638250981278816 5 11 -0.3938619841607931 5 12 -0.35653698222356667 5 13 -0.6737700467847318 5 14 0.15763337174004732 5 15 -0.08950393061022766 5 16 0.4354411902753199 5 17 -0.2248870098263538 5 18 0.2653663835067803 5 19 -0.13107926321801955 5 20 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-0.20541165079475832 8 14 -0.4935257761296299 8 15 -0.2563272388808997 8 16 0.3364436965409823 8 17 0.5899626142337979 8 18 0.24061701007319414 8 19 0.4047916641002729 8 20 1.0244803624365986 8 21 0.23193637150798452 8 22 0.24689147073525675 8 23 0.74167860476296 8 24 0.6067085929206328 8 25 0.6503605081151704 8 26 1.6611632066119277 8 27 1.166290999440701 8 28 1.0049194460083264 8 29 2.178082609955702 8 30 1.7560373850922133 8 31 0.16768935865017198 8 32 0.13622941382181963 8 33 0.49363910056471383 8 34 0.5660862955502672 8 35 0.26910073423469427 8 36 0.9593354630686513 8 37 0.8519034414671012 8 38 1.0547274198365422 8 39 1.2964927347771003 8 40 1.2199827147448765 8 41 1.2305837264692947 8 42 1.1112392446268693 8 43 1.34853814559672 8 44 1.5302087014500536 8 45 1.5714117188235157 8 46 1.6976689757124603 8 47 1.8829836350016471 8 48 1.2580872717462945 8 49 1.3874031777446412 8 50 1.96278984853992 8 51 1.9331749300260839 8 52 2.090966835386002 8 53 2.0494273831743683 8 54 2.689790960816378 8 55 2.0602514309176954 8 56 0.7636714558675255 9 1 -0.4295583571161856 9 2 0.07715951218485628 9 3 -0.5466042712018719 9 4 -0.3888998852351051 9 5 -0.532590073112182 9 6 -0.6823110800822647 9 7 -0.7113016628889284 9 8 -0.3916464262936259 9 9 -0.8345178113930274 9 10 -1.869059442971377 9 11 -0.6767092973980127 9 12 -0.9210848197071826 9 13 -0.7846809248974438 9 14 -1.1832086990332158 9 15 -1.3140288323140297 9 16 -1.2762232920313679 9 17 -0.9298345851867751 9 18 -0.7875497370698881 9 19 -0.3662529198366329 9 20 -0.7455985044554012 9 21 -0.2965522813167425 9 22 -0.28723053151808386 9 23 0.3594960842414707 9 24 -0.2190599568942737 9 25 -0.10724451361346254 9 26 -0.3673254462127957 9 27 -0.8807072300780483 9 28 -0.3391172512393794 9 29 0.652330155424881 9 30 0.18248193683855263 9 31 0.16897698137671213 9 32 0.023723378090291902 9 33 0.5107805781117918 9 34 -0.047868315748619494 9 35 -0.6752095828424025 9 36 0.5562290732408712 9 37 0.5467368552768548 9 38 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46 -0.6482172916801616 120 47 -0.6796297951253933 120 48 -0.5655666907461452 120 49 -0.6773276147538461 120 50 -0.9010939990099871 120 51 -0.792929123210456 120 52 -1.2021366128898592 120 53 -1.0307299066017954 120 54 -0.8968696562435134 120 55 -1.1811278794271338 120 56 -0.585088374743485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 422.0 3 114.0 4 261.0 5 237.0 6 575.0 7 1819.0 8 908.0 9 1272.0 10 1764.0 11 1216.0 12 1414.0 13 1966.0 14 1699.0 15 1680.0 16 1961.0 17 2357.0 18 2776.0 19 3308.0 20 4384.0 21 4175.0 22 2680.0 23 2880.0 24 2295.0 25 1903.0 26 1346.0 27 1392.0 28 1563.0 29 1913.0 30 2226.0 31 2733.0 32 3353.0 33 4108.0 34 4996.0 35 5907.0 36 7598.0 37 11691.0 38 12677.0 39 8041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 29.803714461755128 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18.27055955073982 58.20401552693889 11.286957899462191 12.238467022859105 24 12.632801978716474 61.37982026071877 14.470684540837434 11.51669321972731 25 13.059708062613351 20.68300670857324 17.0531579596077 49.20412726920571 26 52.36144514628538 19.10828025477707 14.255006122754624 14.275268476182926 27 14.826106494902588 16.953184890165566 15.545118910202751 52.6755897047291 28 15.825886168543882 54.72035675885287 16.975129113567057 12.478627959036187 29 49.12707649984129 19.84975134906359 20.781751490142135 10.241420660952985 30 14.813834480324687 54.18828304217399 22.53485089112405 8.463031586377271 31 47.51595279913153 21.906745628977166 22.760209350149598 7.817092221741702 32 17.751688219976167 22.64818819790793 50.017213223286404 9.582910358829501 33 36.425934756883734 18.672177184337414 22.776806372419152 22.125081686359703 34 24.825387855294874 20.089888831003698 24.466009130162206 30.61871418353922 35 17.019866964656412 49.4448026995751 23.73700343630469 9.798326899463795 36 32.24400678877024 24.380347924474933 22.491337246305072 20.884308040449756 37 14.261352089279244 25.51757044524754 46.72669276240799 13.494384703065219 38 29.013431898769998 21.175357461822106 36.58799706425913 13.223213575148776 39 16.810260946483858 21.95842547545334 34.3343653250774 26.896948252985403 40 17.909271962823635 22.98738658995353 45.936711661872096 13.166629785350741 41 29.129522578589075 21.51804605129204 39.09136781721125 10.261063552907641 42 32.40105213748639 21.602472700220524 36.409447982074695 9.587027180218398 43 17.74730725623583 30.334998582766442 38.99518140589569 12.922512755102042 44 15.353751859987247 25.950364911783463 38.936618720328774 19.759264507900518 45 17.213579503171623 33.331266168184555 37.830894078457774 11.624260250186044 46 16.386673755094808 25.641502746765905 44.95481127060074 13.017012227538544 47 27.002278187024086 23.84206933843931 35.94305418893883 13.21259828559778 48 28.613619029036663 24.204906256097345 34.515848661688274 12.665626053177714 49 30.734863930502616 23.932275507490473 34.139703926956834 11.193156635050086 50 16.885581758356764 31.172563036016783 39.47478115881617 12.467074046810286 51 16.327381057932723 34.264891032574965 36.43891972407923 12.968808185413083 52 25.016633399866933 26.09181636726547 37.61898425371479 11.272565979152805 53 14.123376623376624 27.08732524306295 39.32208501880633 19.4672131147541 54 13.496154221484904 33.678728897011204 37.124404503153805 15.700712378350085 55 24.38532956986384 25.062576556425416 36.1062292520992 14.445864621611545 56 19.236162885646323 28.92480562360209 39.22941740334434 12.60961408740725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 269.5 11 539.0 12 517.0 13 434.5 14 374.0 15 348.5 16 323.0 17 472.5 18 622.0 19 817.0 20 1012.0 21 1016.5 22 1174.5 23 1328.0 24 1639.5 25 1951.0 26 2408.5 27 2866.0 28 3095.0 29 3794.5 30 4265.0 31 4696.5 32 5128.0 33 4414.5 34 3701.0 35 8458.5 36 13216.0 37 8231.0 38 2769.5 39 2293.0 40 2128.0 41 1963.0 42 2005.0 43 2047.0 44 2490.5 45 2934.0 46 3624.0 47 3660.5 48 3007.0 49 2821.0 50 2635.0 51 3442.0 52 4249.0 53 5579.5 54 7552.5 55 8195.0 56 8120.5 57 8046.0 58 7070.0 59 6094.0 60 5590.5 61 5087.0 62 4466.5 63 3322.0 64 2798.0 65 2434.5 66 2071.0 67 1789.5 68 1508.0 69 1337.5 70 1167.0 71 889.0 72 433.0 73 255.0 74 174.5 75 94.0 76 62.0 77 30.0 78 23.0 79 11.0 80 6.0 81 6.0 82 6.0 83 5.5 84 5.0 85 6.0 86 7.0 87 6.0 88 2.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0017604084147522226 23 8.802042073761113E-4 24 8.802042073761113E-4 25 0.02112490097702667 26 0.08714021653023502 27 0.10650470909250946 28 0.12674940586216002 29 0.17428043306047003 30 0.23765513599155003 31 0.27022269166446616 32 0.2860663673972362 33 0.3274359651439134 34 0.3159933104480239 35 0.3582431124020773 36 0.4242584279552856 37 0.38464923862336065 38 0.458586392042954 39 0.49291435613062234 40 0.5589296716838307 41 0.5712525305870961 42 0.6135023325411495 43 0.6284658040665434 44 0.6214241704075345 45 0.6460698882140656 46 0.6601531555320834 47 0.7050435701082651 48 0.7534548015139513 49 0.704163365900889 50 0.7543350057213273 51 0.7252882668779157 52 0.7789807235278585 53 0.7763401109057301 54 0.7825015403573629 55 0.8344335885925533 56 0.8291523633482968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 113610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.089076665786465 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37668850898709 38.652407358507176 2 9.958999890178276 9.578382184666841 3 3.8510817439689573 5.555848956958014 4 1.7425046674232163 3.3518176216882316 5 0.9481275396273383 2.2797288971041283 6 0.6351356298275799 1.8325851597570635 7 0.47772449390489435 1.6081330868761554 8 0.31299190979975844 1.20411935569052 9 0.2745543068418933 1.1882756799577503 >10 1.2483069151078083 10.798345216090134 >50 0.08785737818940587 2.902033271719039 >100 0.06955375773327964 5.72660857318898 >500 0.009151810228063112 2.919637355866561 >1k 0.005491086136837867 3.979403221547399 >5k 0.0018303620456126223 8.42267406038201 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 9569 8.42267406038201 No Hit GGACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1687 1.4849044978434998 No Hit GGACATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1502 1.3220667194789193 No Hit GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 1332 1.1724320042249803 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGT 850 0.7481735762696945 No Hit GGACATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 659 0.5800545726608574 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGG 624 0.5492474254026933 No Hit GGACATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 606 0.5334037496699234 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 578 0.5087580318633923 Illumina Single End Adapter 2 (95% over 23bp) GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 475 0.41809699850365284 Illumina Single End Adapter 2 (96% over 30bp) GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 450 0.39609189331925004 No Hit GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 329 0.28958718422674057 No Hit GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 306 0.26934248745709005 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT 291 0.2561394243464484 Illumina Single End Adapter 2 (100% over 29bp) GGACATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 223 0.1962855382448728 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA 217 0.19100431300061613 No Hit GGACATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 216 0.19012410879324004 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT 189 0.16635859519408502 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC 184 0.16195757415720446 Illumina Single End Adapter 2 (96% over 28bp) GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 0.16195757415720446 No Hit GGACATGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC 159 0.1399524689728017 No Hit GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 156 0.13731185635067333 No Hit GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA 153 0.13467124372854503 Illumina Single End Adapter 2 (97% over 34bp) GGACATGGGTGGGAATTCCTTCGGCAACATTATCTGGTTGATGCTGAAAAACATCG 153 0.13467124372854503 No Hit GGACATGGGGGGAATTCCTTCGGCAACATCAACTGGTTGATGCTGAAAAACATCGA 151 0.1329108353137928 No Hit GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.13115042689904058 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCC 147 0.12939001848428835 Illumina Single End Adapter 2 (100% over 22bp) GGACATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT 141 0.12410879324003168 No Hit GGACATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 134 0.1179473637883989 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGAGTTTTTTTTTTTTTTTTTTTT 134 0.1179473637883989 No Hit GGACATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC 132 0.11618695537364669 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGG 131 0.11530675116627057 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGGACTTTTTTTTTTTTTTTTTTTT 129 0.11354634275151836 No Hit GGACATGGGTAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT 125 0.11002552592201391 No Hit GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.10826511750726169 No Hit GGACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.10738491329988559 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGGACATGGGAAGCAGT 122 0.10738491329988559 No Hit GGACATGGGAAGCAGTGGTATCAACGCGAACGGAAGAGCTCGTATGCCGTCTTCTG 115 0.10122348384825279 Illumina Single End Adapter 1 (96% over 29bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.08537980811548279 0.0 0.0 0.0 0.0 11 0.18308247513423115 0.0 0.0 0.0 0.0 12 0.19012410879324004 0.0 0.0 0.0 0.0 13 0.19100431300061615 0.0 0.0 0.0 0.0 14 0.29046738843411674 0.0 0.0 0.0 0.0 15 0.2957486136783734 0.0 0.0 0.0 0.0 16 0.3071912683742628 0.0 0.0 0.0 0.0 17 0.31159228941114336 0.0 0.0 0.0 0.0 18 0.31687351465540003 0.0 0.0 0.0 0.0 19 0.32567555672916115 0.0 0.0 0.0 0.0 20 0.38817005545286504 0.0 0.0 0.0 0.0 21 0.3996127101487545 0.0 0.0 0.0 0.0 22 0.4233782237479095 0.0 0.0 0.0 0.0 23 0.466508229909339 0.0 0.0 0.0 0.0 24 0.5228412991814101 0.0 0.0 0.0 0.0 25 0.5404453833289323 0.0 0.0 0.0 0.0 26 0.5466068127805651 0.0 0.0 0.0 0.0 27 0.5554088548543262 0.0 0.0 0.0 0.0 28 0.8828448199982396 0.0 0.0 0.0 0.0 29 0.8951676789015052 0.0 0.0 0.0 0.0 30 1.0324795352521785 0.0 0.0 0.0 0.0 31 1.0412815773259396 0.0 0.0 0.0 0.0 32 1.0500836193997007 0.0 0.0 0.0 0.0 33 1.060646069888214 0.0 0.0 0.0 0.0 34 1.0703283161693513 0.0 0.0 0.0 0.0 35 1.407446527594402 0.0 0.0 0.0 0.0 36 1.432092245400933 0.0 0.0 0.0 0.0 37 1.4444151043041986 0.0 0.0 0.0 0.0 38 1.4523369421705836 0.0 0.0 0.0 0.0 39 1.4620191884517209 0.0 0.0 0.0 0.0 40 1.4743420473549864 0.0 0.0 0.0 0.0 41 1.4822638852213714 0.0 0.0 0.0 0.0 42 1.495466948332013 0.0 0.0 0.0 0.0 43 1.5051491946131503 0.0 0.0 0.0 0.0 44 1.5201126661385442 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGCTC 50 7.2759576E-12 50.305916 50 CATCGAA 50 7.2759576E-12 50.305916 50 GCGTGGA 15 0.008211003 50.305912 50 AGGCCAC 15 0.008211003 50.305912 48 GGGTTGC 15 0.008225452 50.283554 47 CGGGTGG 20 4.0530166E-4 50.283554 46 ATCAGAG 15 0.008225452 50.283554 47 CTGGTAG 25 2.0071024E-5 50.283554 46 CTCGTAG 20 4.0530166E-4 50.283554 46 CGTGGGA 35 5.022048E-8 50.26122 44 CGCGGGA 45 1.2732926E-10 50.26122 44 CAAGGGA 55 0.0 50.26122 44 ACAAAGG 15 0.00823992 50.261215 43 TGGCAAT 15 0.00823992 50.261215 42 GTCATGG 25 2.0123905E-5 50.261215 42 TAAGCCG 15 0.00823992 50.261215 43 TATGCGG 25 2.0123905E-5 50.261215 43 CGAAGGC 15 0.00823992 50.261215 43 GCGTGGG 25 2.0123905E-5 50.261215 43 CGTGGGG 15 0.00823992 50.261215 44 >>END_MODULE