FastQCFastQC Report
Tue 31 May 2016
SRR212512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34842
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8752.511336892256472No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3460.993054359680845No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2540.7290052235807359No Hit
CTGTGTGGGAGAGTGCAGTACTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCCTTT2520.723265024969864No Hit
CTGTGTGGGATTTGGCTCTGGTTATCTTCAGGGATGCTGTTGGCTCATGATCCAAACT1700.4879168819241146No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1630.46782618678606286No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1550.44486539234257505No Hit
CTGTGTGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1530.4391251937317031No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1490.42764479650995924No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1480.42477469720452327No Hit
CTGTGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1110.31858102290339246No Hit
CTGTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1040.2984903277653407No Hit
CTGTGTGGGAAACAAATGGTTCTGGTCTAACTGGATGTGTGCATGTAGAAGGATGCAA1020.29275012915446874No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1000.2870099305435968No Hit
CTGTGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA950.27265943401641696No Hit
CTGTGTGGGAGTTTTTTATAAAAATATATATTTTTGTCCAAAAAAAAGGCATACATAT920.26404913610010905No Hit
CTGTGTGGGAACAGAGAACTGTAGATTTCAAGAGCTCACATTTAAAGACAGCTGTTCA890.25543883818380114No Hit
CTGTGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCCAA830.23821824235118536No Hit
CTGTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG750.2152574479076976No Hit
CTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT750.2152574479076976No Hit
CTGTGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGG730.20951724929682566No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.19229665346420985No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC610.17507605763159406No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC590.16933585902072212No Hit
CTGTGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA590.16933585902072212No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG580.16646575971528613No Hit
CTGTGTGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT570.16359566040985019No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG530.1521152631881063No Hit
CTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCCGAGTGCTGAG520.14924516388267034No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.14637506457723437No Hit
CTGTGTGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG510.14637506457723437No Hit
CTGTGTGGGTAAGTTGGGAATCTTTACTCTCTCTTCTATACCTATTATCCATAGGTTT500.1435049652717984No Hit
CTGTGTGGGGGAGCCGCCGTGGGTAGGGTTAGTGTGAGAGGTGCGGCGGGGGGGGTCT480.13776476666092646No Hit
CTGTGTGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACC460.13202456805005452No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.13202456805005452No Hit
CTGTGTGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC450.12915446874461856No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA430.12341427013374663No Hit
CTGTGTGGGGTCACCAGGGGGCTGGAGCGATGGCTCAGTGGTTAAGAGCACTGACTGC420.12054417082831066No Hit
CTGTGTGGGGTGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC400.11480397221743871No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.11480397221743871No Hit
CTGTGTGGGATCTAAAAAATAAATAAATAAATAAGATCCAAGGTAAAATTTTCCAATT400.11480397221743871No Hit
CTGTGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA390.11193387291200274No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC390.11193387291200274No Hit
CTGTGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG390.11193387291200274No Hit
CTGTGTGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC390.11193387291200274No Hit
CTGTGTGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA370.10619367430113082No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10619367430113082No Hit
CTGTGTGGGGGCGCCACAGTCCTCTACCCCTGCGTGGTGTATGACCGTGGGCCCGAGA370.10619367430113082No Hit
CTGTGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG360.10332357499569485No Hit
CTGTGTGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTGCTGAAGG360.10332357499569485No Hit
CTGTGTGGGGCCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGCGGTGCAGATTTCC360.10332357499569485No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC360.10332357499569485No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT350.10045347569025889No Hit
CTGTGTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC350.10045347569025889No Hit
CTGTGTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC350.10045347569025889No Hit
CTGTGTGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACTT350.10045347569025889No Hit
CTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC350.10045347569025889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTCC251.621982E-552.00000452
CTTTTGG150.007161753752.012
ACAAATG150.007161753752.012
CTTTTCG150.007161753752.011
GGAGACC150.007161753752.027
GTGTCTC150.007161753752.025
ACCGGAC150.007161753752.035
AACAAGC150.007161753752.050
TAGGATT150.007161753752.034
CCGGACA150.007161753752.036
CACACGC150.007161753752.036
TCGATTC650.052.020
TAAACCC150.007161753752.048
CACACAT150.007161753752.016
ACATGCA150.007161753752.019
AGGATTT150.007161753752.035
TGACCCT150.007161753752.016
ACGCATG150.007161753752.025
TCGGCTT150.007161753752.015
TTCCGCG150.007161753752.023
Produced by FastQC (version 0.11.5)