FastQCFastQC Report
Tue 31 May 2016
SRR212511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37592
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC8942.378165567141945No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3310.8805064907427113No Hit
CTGTGTGGGAGAGTGCAGTACTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCCTTT2730.7262183443285806No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2560.6809959565865078No Hit
CTGTGTGGGATTTGGCTCTGGTTATCTTCAGGGATGCTGTTGGCTCATGATCCAAACT1880.5001064056182166No Hit
CTGTGTGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1800.47882528197488833No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1720.4575441583315599No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1580.42030219195573526No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1540.4096616301340711No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1270.33783783783783783No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1190.3165567141945095No Hit
CTGTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1130.3005958714620132No Hit
CTGTGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1090.28995530964034905No Hit
CTGTGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1030.27399446690785273No Hit
CTGTGTGGGAGTTTTTTATAAAAATATATATTTTTGTCCAAAAAAAAGGCATACATAT960.2553734837199404No Hit
CTGTGTGGGAAACAAATGGTTCTGGTCTAACTGGATGTGTGCATGTAGAAGGATGCAA930.24739306235369227No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG920.24473292189827622No Hit
CTGTGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCCAA880.23409236007661205No Hit
CTGTGTGGGAACAGAGAACTGTAGATTTCAAGAGCTCACATTTAAAGACAGCTGTTCA830.2207916577995318No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC820.2181315173441158No Hit
CTGTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG820.2181315173441158No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.21281123643328367No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1862098318791232No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG690.18354969142370717No Hit
CTGTGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGG640.17024898914662695No Hit
CTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT620.16492870823579486No Hit
CTGTGTGGGGTGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC610.1622685677803788No Hit
CTGTGTGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC600.15960842732496275No Hit
CTGTGTGGGTAAGTTGGGAATCTTTACTCTCTCTTCTATACCTATTATCCATAGGTTT580.15428814641413066No Hit
CTGTGTGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC570.15162800595871462No Hit
CTGTGTGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA560.14896786550329857No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC550.14630772504788253No Hit
CTGTGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA520.1383273036816344No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA490.13034688231538624No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA490.13034688231538624No Hit
CTGTGTGGGGGAGCCGCCGTGGGTAGGGTTAGTGTGAGAGGTGCGGCGGGGGGGGTCT490.13034688231538624No Hit
CTGTGTGGGGAACATGTTTTTACAAGTAAACTCGCACTGGGCAAAGTCAATATAGACA480.1276867418599702No Hit
CTGTGTGGGGTCACCAGGGGGCTGGAGCGATGGCTCAGTGGTTAAGAGCACTGACTGC460.12236646094913811No Hit
CTGTGTGGGGCCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGCGGTGCAGATTTCC460.12236646094913811No Hit
CTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC460.12236646094913811No Hit
CTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC450.11970632049372208No Hit
CTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCCGAGTGCTGAG440.11704618003830602No Hit
CTGTGTGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT440.11704618003830602No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT430.11438603958288998No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11438603958288998No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC420.11172589912747392No Hit
CTGTGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA410.1090657586720579No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA390.1037454777612258No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG390.1037454777612258No Hit
CTGTGTGGGGGCGCCACAGTCCTCTACCCCTGCGTGGTGTATGACCGTGGGCCCGAGA380.10108533730580974No Hit
CTGTGTGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG380.10108533730580974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATG1000.052.06675736
GTAGAGC150.007129742852.06675740
CATGATC251.6121021E-552.06675746
TTGTCAT150.007129742852.06675744
CCTATTG150.007129742852.06675740
TCAAGGA150.007129742852.06675741
ATCCATA150.007129742852.06675747
CTATACC150.007129742852.06675736
TATACCT150.007129742852.06675737
GCGACTG150.007129742852.06675752
AAACATC1000.052.06675748
ATAGGTT150.007129742852.06675751
GGTCCAC150.007129742852.06675740
TTATCCA150.007129742852.06675745
TGTCATC150.007129742852.06675745
TATTGTC150.007129742852.06675742
CGTAGAG150.007129742852.06675739
ACATCGA1000.052.06675750
AGAACCA150.007129742852.06675737
TATCCAT150.007129742852.06675746
Produced by FastQC (version 0.11.5)