FastQCFastQC Report
Tue 31 May 2016
SRR212510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39337
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7972.0260823143605258No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3260.8287363042428247No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2520.6406182474515087No Hit
CTGTGTGGGAGAGTGCAGTACTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCCTTT2410.6126547525230699No Hit
CTGTGTGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1750.4448737829524366No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1560.39657320080331493No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1520.3864046571929735No Hit
CTGTGTGGGATTTGGCTCTGGTTATCTTCAGGGATGCTGTTGGCTCATGATCCAAACT1460.37115184177746147No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1270.32285125962833977No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1260.3203091237257544No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1110.2821770851869741No Hit
CTGTGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1100.2796349492843887No Hit
CTGTGTGGGAAACAAATGGTTCTGGTCTAACTGGATGTGTGCATGTAGAAGGATGCAA1040.26438213386887666No Hit
CTGTGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCCAA980.24912931845336453No Hit
CTGTGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA930.23641863894043774No Hit
CTGTGTGGGAGTTTTTTATAAAAATATATATTTTTGTCCAAAAAAAAGGCATACATAT890.22625009533009635No Hit
CTGTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA860.2186236876223403No Hit
CTGTGTGGGAACAGAGAACTGTAGATTTCAAGAGCTCACATTTAAAGACAGCTGTTCA840.2135394158171696No Hit
CTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT810.20591300810941354No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC800.20337087220682817No Hit
CTGTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG780.19828660040165746No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC660.16778096957063326No Hit
CTGTGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGG650.1652388336680479No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1601545618628772No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA600.15252815415512114No Hit
CTGTGTGGGTAAGTTGGGAATCTTTACTCTCTCTTCTATACCTATTATCCATAGGTTT590.1499860182525358No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG560.14235961054477972No Hit
CTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCCGAGTGCTGAG540.13727533873960904No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG540.13727533873960904No Hit
CTGTGTGGGGAACATGTTTTTACAAGTAAACTCGCACTGGGCAAAGTCAATATAGACA520.13219106693443833No Hit
CTGTGTGGGGGAGCCGCCGTGGGTAGGGTTAGTGTGAGAGGTGCGGCGGGGGGGGTCT520.13219106693443833No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.12964893103185296No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT480.1220225233240969No Hit
CTGTGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA480.1220225233240969No Hit
CTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC480.1220225233240969No Hit
CTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC480.1220225233240969No Hit
CTGTGTGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC480.1220225233240969No Hit
CTGTGTGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC480.1220225233240969No Hit
CTGTGTGGGGTGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC460.1169382515189262No Hit
CTGTGTGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA450.11439611561634086No Hit
CTGTGTGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA450.11439611561634086No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC430.10931184381117015No Hit
CTGTGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC420.1067697079085848No Hit
CTGTGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC410.10422757200599943No Hit
CTGTGTGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACC410.10422757200599943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAG550.052.359449
GGGGGAC203.2925393E-452.359448
CCATCTC203.2925393E-452.3594410
TGGGACC203.2925393E-452.359446
GGAGCCT203.2925393E-452.359448
GGGCCAT203.2925393E-452.359447
TGGGATT700.052.3594366
GAGGCAT150.00697553752.35943210
GGGTCGG150.00697553752.3594327
GTTTCCT600.052.3594329
GGGATTG150.00697553752.3594327
GAGAGTG600.052.3594329
TGGGATA458.185452E-1152.3594326
GGGGACC307.433955E-752.3594327
GGTCGGG150.00697553752.3594328
TAAGGAG203.3770647E-452.09195739
GGGTCAC150.00711852552.09195344
CTTTAAC150.00711852552.09195344
CCGTTGT353.7036443E-852.09195338
TGCCTCT150.00711852552.09195344