SRR212509_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212509_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64826
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1024	1.5796131181933175	No Hit
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	792	1.221732021102644	No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	605	0.9332675161200753	No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	512	0.7898065590966588	No Hit
CTGTGTGGGAGAGTGCAGTACTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCCT	424	0.6540585567519205	No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	391	0.6031530558726437	No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	332	0.5121401906642397	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	313	0.4828309628852621	No Hit
CTGTGTGGGATTTGGCTCTGGTTATCTTCAGGGATGCTGTTGGCTCATGATCCAAA	307	0.4735754172708481	No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	248	0.38256255206244405	No Hit
CTGTGTGGGAACAGAGAACTGTAGATTTCAAGAGCTCACATTTAAAGACAGCTGTT	218	0.33628482399037424	No Hit
CTGTGTGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	213	0.3285718693116959	No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	166	0.25607009533211983	No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	166	0.25607009533211983	No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	163	0.25144232252491283	No Hit
CTGTGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCC	140	0.21596273100299263	No Hit
CTGTGTGGGAGACATTATTTGAAGATTCCTTCCAACAGATTATGGCATTAAAACCA	138	0.21287754913152132	No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	137	0.21133495819578565	No Hit
CTGTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	121	0.18665350322401505	No Hit
CTGTGTGGGAAACAAATGGTTCTGGTCTAACTGGATGTGTGCATGTAGAAGGATGC	121	0.18665350322401505	No Hit
CTGTGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	117	0.18048313948107242	No Hit
CTGTGTGGGTAAGTTGGGAATCTTTACTCTCTCTTCTATACCTATTATCCATAGGT	113	0.17431277573812975	No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	110	0.16968500293092278	No Hit
CTGTGTGGGAGTTTTTTATAAAAATATATATTTTTGTCCAAAAAAAAGGCATACAT	109	0.16814241199518712	No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	104	0.16042945731650882	No Hit
CTGTGTGGGAGCCTTGGGTGTTTCCCCACTCTTGAAGCCCAGGGCCCGGGACCTAG	102	0.15734427544503748	No Hit
CTGTGTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	97	0.14963132076635918	No Hit
CTGTGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA	97	0.14963132076635918	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	94	0.1450035479591522	No Hit
CTGTGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGT	91	0.1403757751519452	No Hit
CTGTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	89	0.13729059328047388	No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	88	0.13574800234473822	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	87	0.13420541140900258	No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	81	0.12494986579458858	No Hit
CTGTGTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	81	0.12494986579458858	No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	81	0.12494986579458858	No Hit
CTGTGTGGGGATTATTTTCTCTACCACCTCTGTGGTAGTCTTTCAGTCTTTTGCAC	80	0.12340727485885294	No Hit
CTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	78	0.1203220929873816	No Hit
CTGTGTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	73	0.11260913830870331	No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC	71	0.10952395643723198	No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	71	0.10952395643723198	No Hit
CTGTGTGGGTGTTGAATGTAACTTTATTTTTGTGTGCAAACACTAAATTTTTAATT	70	0.10798136550149631	No Hit
CTGTGTGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC	70	0.10798136550149631	No Hit
CTGTGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	69	0.10643877456576066	No Hit
CTGTGTGGGGTGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	67	0.10335359269428933	No Hit
CTGTGTGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	66	0.10181100175855366	No Hit
CTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG	65	0.10026841082281801	No Hit
CTGTGTGGGAACGGGCGGTCTACACAGCTGCAAAATGAGATCGACATGAGATTCCC	65	0.10026841082281801	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCAAA	25	1.9959987E-5	50.289497	50
ATTATAT	15	0.00820566	50.289497	50
AGGATGC	20	4.037577E-4	50.289494	50
TGCCTAA	20	4.037577E-4	50.289494	50
AAGAAAC	65	0.0	50.250393	49
CCGGAAG	70	0.0	50.25039	49
GGACCTA	15	0.008230957	50.25039	49
TAAAACC	25	2.0052223E-5	50.25039	49
GATCCAA	30	9.974174E-7	50.25039	49
GTGCCTA	20	4.0531278E-4	50.250385	49
TTATGGC	25	2.0237752E-5	50.17236	40
TGGCATT	25	2.0237752E-5	50.17236	43
CCGGAGC	25	2.0237752E-5	50.17236	40
CATTGAC	30	1.0081894E-6	50.17236	41
ACGCCAC	15	0.008281726	50.17236	41
TCAAGGA	15	0.008281726	50.17236	41
ACAGATT	25	2.0237752E-5	50.17236	35
TCGACAT	15	0.008281726	50.17236	41
TGACCGT	15	0.008281726	50.17236	42
CCCGGGA	15	0.008281726	50.17236	45

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content