FastQCFastQC Report
Tue 31 May 2016
SRR212508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59726
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC10311.7262163881726549No Hit
CTGTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9121.5269731775106319No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5330.8924086662425075No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5060.8472022234872584No Hit
CTGTGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA4100.6864682048019288No Hit
CTGTGTGGGAGAGTGCAGTACTCTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGCCT4020.673073703244818No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3670.6144727589324582No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2860.4788534306667113No Hit
CTGTGTGGGATTTGGCTCTGGTTATCTTCAGGGATGCTGTTGGCTCATGATCCAAA2650.4436928640792955No Hit
CTGTGTGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2340.39178917054549106No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2160.36165154204199174No Hit
CTGTGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1960.32816528814921475No Hit
CTGTGTGGGAACAGAGAACTGTAGATTTCAAGAGCTCACATTTAAAGACAGCTGTT1820.30472491042427086No Hit
CTGTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1440.2411010280279945No Hit
CTGTGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCC1340.22435790108160603No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1320.2210092756923283No Hit
CTGTGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1290.21598633760841177No Hit
CTGTGTGGGAGACATTATTTGAAGATTCCTTCCAACAGATTATGGCATTAAAACCA1280.21431202491377288No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.21431202491377288No Hit
CTGTGTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1270.21263771221913405No Hit
CTGTGTGGGAAACAAATGGTTCTGGTCTAACTGGATGTGTGCATGTAGAAGGATGC1240.2076147741352175No Hit
CTGTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1150.19254595988346782No Hit
CTGTGTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1080.18082577102099587No Hit
CTGTGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA1030.17245420754780164No Hit
CTGTGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1000.1674312694638851No Hit
CTGTGTGGGTAAGTTGGGAATCTTTACTCTCTCTTCTATACCTATTATCCATAGGT980.16408264407460738No Hit
CTGTGTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC970.16240833137996852No Hit
CTGTGTGGGATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGT920.15403676790677429No Hit
CTGTGTGGGAGCCTTGGGTGTTTCCCCACTCTTGAAGCCCAGGGCCCGGGACCTAG920.15403676790677429No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA900.15068814251749657No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC890.1490138298228577No Hit
CTGTGTGGGAGTTTTTTATAAAAATATATATTTTTGTCCAAAAAAAAGGCATACAT880.14733951712821886No Hit
CTGTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG880.14733951712821886No Hit
CTGTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC850.1423165790443023No Hit
CTGTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT850.1423165790443023No Hit
CTGTGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAA780.13059639018183036No Hit
CTGTGTGGGGATTATTTTCTCTACCACCTCTGTGGTAGTCTTTCAGTCTTTTGCAC760.12724776479255265No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA760.12724776479255265No Hit
CTGTGTGGGTGTTGAATGTAACTTTATTTTTGTGTGCAAACACTAAATTTTTAATT740.12389913940327496No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA740.12389913940327496No Hit
CTGTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG730.1222248267086361No Hit
CTGTGTGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTA700.11720188862471956No Hit
CTGTGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG680.11385326323544184No Hit
CTGTGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC650.1088303251515253No Hit
CTGTGTGGGGGAGCCGCCGTGGGTAGGGTTAGTGTGAGAGGTGCGGCGGGGGGGGT650.1088303251515253No Hit
CTGTGTGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG650.1088303251515253No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA650.1088303251515253No Hit
CTGTGTGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCCTCCCGAGTGCTG630.10548169976224761No Hit
CTGTGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG630.10548169976224761No Hit
CTGTGTGGGGTGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT630.10548169976224761No Hit
CTGTGTGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTG620.10380738706760875No Hit
CTGTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC610.1021330743729699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAAA550.050.29476550
GATCCAA550.050.29476549
GACCTAG251.9928379E-550.29476550
CCGGAAG402.4774636E-950.2947649
CTGATAC150.00819909350.2947649
ACATCGA950.050.2947650
CATAGGT150.00819909350.2947650
CCATAGG150.00819909350.2947649
CAGCTGT402.4774636E-950.2947649
TGATCCA550.050.20995348
CGGGACC252.012875E-550.20995347
CAAGAAC150.00825403950.2099546
ATCCATA150.00825403950.2099547
CGCCTGA150.00825403950.2099546
GCGAATG150.00825403950.2099546
CCTGATA150.00825403950.2099548
CCAGAGA150.00825403950.2099547
TATCCAT150.00825403950.2099546
CGAATGA150.00825403950.2099547
AAGGCAT150.00825403950.2099546
Produced by FastQC (version 0.11.5)