FastQCFastQC Report
Tue 31 May 2016
SRR212507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40585
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5241.2911174079093262No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3760.9264506591105087No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3220.7933965750893187No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3090.7613650363434766No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3070.7564371073056548No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3020.7441172847111002No Hit
TTGGACGGGAAGAGAGAAATTGTATCCCCGTGCATACTATTTAGTGCATTCCCATGCA2910.7170136750030799No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2530.6233830232844647No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2000.49279290378218554No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1910.4706172231119872No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1810.4459775779228779No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1600.3942343230257484No Hit
TTGGACGGGATCTTATGAATGCTACAAATATGTAATGTTGGAAGAGTATATTATTTGT1370.3375631390907971No Hit
TTGGACGGGAGGTATTTGTAGTTTACCCTAATGCTTCTATAGAAGGAAGTAGGAACAA1240.305531600344955No Hit
TTGGACGGGGGAGCAGGCAGCCTAGGCCTGGGACTGGAACAGCGCGGAGATGGGGGCG1010.2488604164100037No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC930.22914870025871628No Hit
TTGGACGGGGGGCGTCGGCTGTCGGAGGAGCTAAGTCCTGACATGTCTCCAGCTGGGG920.22668473573980535No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA890.21929284218307254No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC840.20697301958851794No Hit
TTGGACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT820.20204509055069605No Hit
TTGGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT800.1971171615128742No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA800.1971171615128742No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC740.18233337439940864No Hit
TTGGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC720.17740544536158678No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.17247751632376493No Hit
TTGGACGGGCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGGCA690.170013551804854No Hit
TTGGACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT690.170013551804854No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC690.170013551804854No Hit
TTGGACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC680.16754958728594307No Hit
TTGGACGGGAGCCCAAAACAATACAGAAAATCAGTCACCTTTGCTGTCTATCAGTGGC680.16754958728594307No Hit
TTGGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT660.16262165824812125No Hit
TTGGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT660.16262165824812125No Hit
TTGGACGGGTGCTTCAGGGCTTGCCTAGTGTCCTCTCCTCATTGGTAGCTCTCACTAG660.16262165824812125No Hit
TTGGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA650.1601576937292103No Hit
TTGGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG650.1601576937292103No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA650.1601576937292103No Hit
TTGGACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT640.15769372921029937No Hit
TTGGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG640.15769372921029937No Hit
TTGGACGGGACGCCAACTTGAAAATGATTTGCAAACCCAGAAAAGGTCACACACTGAC640.15769372921029937No Hit
TTGGACGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAA620.1527658001724775No Hit
TTGGACGGGGAGAGGGGGGAAAAACAAGTAAAAATAAATCGTGTTGTTGAATTTAAAA620.1527658001724775No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA600.14783787113465566No Hit
TTGGACGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA590.14537390661574473No Hit
TTGGACGGGATGCAGTGCTTTGGACGGGAGTTCGTCCCGAATAAGTTCCTGTTACCAG580.1429099420968338No Hit
TTGGACGGGAGAATAACATTAGCCAGTTTAGGTTCCTGTTTTTTCCTTGTTTGAGACA560.13798201305901195No Hit
TTGGACGGGAGCTACCTGAGATCTTGGTGCCTTCAAACTAGGCAGCCATGGAATAAGG540.1330540840211901No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.13059011950227917No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATACTGAAAAACATCGAAC530.13059011950227917No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG530.13059011950227917No Hit
TTGGACGGGGGCAGTAGGGTCGGTCGGCCCTGAGCAGGCAGCCTAGGCCTGGGACTGG530.13059011950227917No Hit
TTGGACGGGACCAAGGACTAAGCATTCAAATTTATGAGCCGATAGGGGCCATTCTCAT520.12812615498336824No Hit
TTGGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA520.12812615498336824No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT520.12812615498336824No Hit
TTGGACGGGGAGGAGGGGTGGGGTCCTGAACATGGCGGACCTCAGCTTTTCTGACGGC520.12812615498336824No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC510.1256621904644573No Hit
TTGGACGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG500.12319822594554639No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA490.12073426142663544No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG490.12073426142663544No Hit
TTGGACGGGAGCAGTGTGTTCGCAGGTTTTTAGACGTCTTGGTGTTTCTAATATTAAC490.12073426142663544No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA480.11827029690772452No Hit
TTGGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT470.11580633238881359No Hit
TTGGACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC470.11580633238881359No Hit
TTGGACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC460.11334236786990268No Hit
TTGGACGGGGGGAAAAAGGGGTAAATTCGAATCAAAAACATTTTTTAGGATATTTCTT460.11334236786990268No Hit
TTGGACGGGAACCAGGAAACTGAACTCTTTCCTGGATTGTGAGTGCTTCGAAGGGACT460.11334236786990268No Hit
TTGGACGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA450.11087840335099175No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA450.11087840335099175No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT450.11087840335099175No Hit
TTGGACGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC450.11087840335099175No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA440.10841443883208082No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA440.10841443883208082No Hit
TTGGACGGGGAACCAAATATTCAATAGTGAGCTGGTGTAGGTTATGTACACAGTGCTT440.10841443883208082No Hit
TTGGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA440.10841443883208082No Hit
TTGGACGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA440.10841443883208082No Hit
TTGGACGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT420.10348650979425897No Hit
TTGGACGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAA420.10348650979425897No Hit
TTGGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG420.10348650979425897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCATG600.052.08405750
TTATTTG150.007124499452.08405751
TCGAACT600.052.08405752
CCATGCA600.052.08405752
ATGAAGC150.007124499452.08405752
AGGAACA150.007124499452.08405751
AACTTTG150.007124499452.08405750
TTCCAAA251.6112606E-552.08405752
TCTGAAG150.007124499452.08405751
TATTTCT150.007124499452.08405751
ATATGGG150.007124499452.08405752
ATGTTTT150.007124499452.08405751
AGATGAA150.007124499452.08405750
ATATTTC150.007124499452.08405750
TGTTACC203.380943E-452.08405350
ACAGCGC203.401674E-452.01975339
ACAAATA150.0071594552.01975324
ATCCCCG600.052.01975324
TAGAACT150.0071594552.01975347
CTACAAA150.0071594552.01975322
Produced by FastQC (version 0.11.5)