##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212507_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40585
Sequences flagged as poor quality	0
Sequence length	58
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.47197240359739	39.0	37.0	39.0	33.0	40.0
2	37.098854256498704	39.0	37.0	39.0	33.0	40.0
3	35.81631144511519	38.0	35.0	39.0	29.0	40.0
4	36.96530737957374	38.0	36.0	39.0	33.0	40.0
5	35.491413083651594	38.0	33.0	39.0	29.0	40.0
6	37.06137735616607	39.0	36.0	39.0	33.0	40.0
7	37.14488111371196	39.0	36.0	39.0	33.0	40.0
8	37.35074534926697	39.0	38.0	39.0	34.0	40.0
9	37.32265615375139	39.0	38.0	39.0	34.0	40.0
10	36.30861155599359	38.0	35.0	39.0	31.0	40.0
11	36.54552174448688	38.0	35.0	39.0	31.0	40.0
12	36.090944930393	38.0	35.0	39.0	30.0	40.0
13	35.949907601330544	38.0	35.0	39.0	30.0	40.0
14	35.86285573487742	38.0	35.0	39.0	30.0	40.0
15	35.95113958359	38.0	35.0	39.0	30.0	40.0
16	35.63284464703708	38.0	35.0	39.0	29.0	40.0
17	35.208549956880624	38.0	33.0	39.0	28.0	40.0
18	35.13748922015523	38.0	33.0	39.0	28.0	40.0
19	35.01264013798201	38.0	33.0	39.0	28.0	40.0
20	34.800714549710484	38.0	33.0	39.0	27.0	40.0
21	34.595983737834175	38.0	33.0	39.0	27.0	40.0
22	34.36754958728594	37.0	33.0	39.0	26.0	40.0
23	34.12903782185536	37.0	33.0	38.0	26.0	40.0
24	34.03856104472096	37.0	33.0	38.0	26.0	40.0
25	34.16986571393372	37.0	33.0	38.0	26.0	40.0
26	33.61488234569422	36.0	33.0	38.0	24.0	40.0
27	33.45760749045214	36.0	32.0	38.0	24.0	39.0
28	33.07837871134656	36.0	31.0	38.0	23.0	39.0
29	33.03355919674757	36.0	31.0	38.0	23.0	39.0
30	32.646618208697795	36.0	31.0	38.0	22.0	39.0
31	31.98750769988912	35.0	30.0	38.0	19.0	39.0
32	31.954786251077984	35.0	30.0	38.0	19.0	39.0
33	31.7067882222496	35.0	29.0	38.0	18.0	39.0
34	31.180189725267955	35.0	29.0	38.0	16.0	39.0
35	30.633066403843785	35.0	28.0	38.0	14.0	39.0
36	31.73083651595417	36.0	30.0	38.0	16.0	39.0
37	31.664531230750278	36.0	30.0	38.0	16.0	39.0
38	31.356683503757544	35.0	30.0	38.0	15.0	39.0
39	31.162005667118393	35.0	29.0	38.0	13.0	39.0
40	31.005691758038683	35.0	29.0	38.0	11.0	39.0
41	31.2461500554392	36.0	30.0	38.0	7.0	39.0
42	31.10481705063447	35.0	30.0	38.0	5.0	39.0
43	30.885524208451397	35.0	29.0	38.0	2.0	39.0
44	30.847283479117902	35.0	29.0	38.0	2.0	39.0
45	30.406701983491438	35.0	29.0	38.0	2.0	39.0
46	30.151730935074536	35.0	28.0	38.0	2.0	39.0
47	29.855242084513982	35.0	28.0	38.0	2.0	39.0
48	29.838511765430578	35.0	28.0	38.0	2.0	39.0
49	29.644449919921154	35.0	27.0	38.0	2.0	39.0
50	29.25585807564371	35.0	27.0	38.0	2.0	39.0
51	28.94079093261057	35.0	26.0	38.0	2.0	39.0
52	28.6819021806086	35.0	26.0	38.0	2.0	39.0
53	28.483380559319947	35.0	26.0	38.0	2.0	39.0
54	28.213995318467415	34.0	25.0	38.0	2.0	39.0
55	28.006209190587654	34.0	24.0	38.0	2.0	39.0
56	27.766662560059135	35.0	23.0	38.0	2.0	39.0
57	27.447455956634226	35.0	23.0	38.0	2.0	39.0
58	26.859923617099913	34.0	20.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
4	1	-1.5770254776446393
4	2	-20.75012952723573
4	3	-13.210053910101529
4	4	-20.766699918594227
4	5	-18.580229590801874
4	6	-10.843814583188646
4	7	-10.430323023603314
4	8	-11.734138725619125
4	9	-11.776818901400667
4	10	-10.21374219560849
4	11	-8.659276651836386
4	12	-7.611388226299965
4	13	-7.527822207032607
4	14	-7.268148666356723
4	15	-7.485138787739928
4	16	-11.240910885208361
4	17	-10.939333340883739
4	18	-10.195769702064204
4	19	-15.844983457737047
4	20	-8.308389107765201
4	21	-2.783365558037527
4	22	-4.599372091671668
4	23	-2.4361026031680986
4	24	-2.4565493021706075
4	25	-1.6755791092945103
4	26	-0.8933431249734092
4	27	-3.0611284396202088
4	28	-0.5668118115939293
4	29	-0.057710557921030414
4	30	0.27752469880034525
4	31	4.934732248135692
4	32	9.228897249952112
4	33	5.256476253473629
4	34	5.683218505595015
4	35	5.3206372665511665
4	36	-6.5927748368763375
4	37	9.093856876458347
4	38	7.0941948700368656
4	39	9.371396547952841
4	40	6.990835782233983
4	41	6.493529221656175
4	42	6.088005217215024
4	43	-3.320343930422961
4	44	-3.111233089309337
4	45	-4.502164525267901
4	46	-2.1175172529437702
4	47	-1.2873949122604422
4	48	3.6568592842319774
4	49	2.60492981595819
4	50	-0.6867397840698466
4	51	7.838285709886474
4	52	9.440539958427003
4	53	2.594743158887148
4	54	14.55259333697828
4	55	9.078553355591882
4	56	9.643230820308283
4	57	9.809007687044236
4	58	10.589282405071394
6	1	-32.57702547764464
6	2	-30.75012952723573
6	3	-28.21005391010153
6	4	-30.766699918594227
6	5	-28.580229590801874
6	6	-30.843814583188646
6	7	-30.430323023603314
6	8	-31.734138725619125
6	9	-31.776818901400667
6	10	-30.21374219560849
6	11	-31.659276651836386
6	12	-30.611388226299965
6	13	-30.527822207032607
6	14	-30.268148666356723
6	15	-30.485138787739928
6	16	-29.24091088520836
6	17	-28.93933334088374
6	18	-28.195769702064204
6	19	-28.844983457737047
6	20	-28.3083891077652
6	21	-27.783365558037527
6	22	-27.59937209167167
6	23	-27.4361026031681
6	24	-27.456549302170608
6	25	-26.67557910929451
6	26	-25.89334312497341
6	27	-26.06112843962021
6	28	-25.56681181159393
6	29	-25.05771055792103
6	30	-24.722475301199655
6	31	-24.065267751864308
6	32	-23.771102750047888
6	33	-23.74352374652637
6	34	-23.316781494404985
6	35	-23.679362733448833
6	36	-24.592774836876337
6	37	-23.906143123541653
6	38	-23.905805129963134
6	39	-23.62860345204716
6	40	-23.009164217766017
6	41	-23.506470778343825
6	42	-23.911994782784976
6	43	-23.32034393042296
6	44	-23.111233089309337
6	45	-22.5021645252679
6	46	-23.11751725294377
6	47	-22.287394912260442
6	48	-22.343140715768023
6	49	-22.39507018404181
6	50	-21.686739784069847
6	51	-21.161714290113526
6	52	-20.559460041572997
6	53	-20.405256841112852
6	54	-20.44740666302172
6	55	-19.921446644408118
6	56	-19.356769179691717
6	57	-19.190992312955764
6	58	-18.410717594928606
11	1	-32.57702547764464
11	2	-30.75012952723573
11	3	-28.21005391010153
11	4	-30.766699918594227
11	5	-28.580229590801874
11	6	-30.843814583188646
11	7	-30.430323023603314
11	8	-31.734138725619125
11	9	-31.776818901400667
11	10	-30.21374219560849
11	11	-31.659276651836386
11	12	-30.611388226299965
11	13	-30.527822207032607
11	14	-30.268148666356723
11	15	-30.485138787739928
11	16	-29.24091088520836
11	17	-28.93933334088374
11	18	-28.195769702064204
11	19	-28.844983457737047
11	20	-28.3083891077652
11	21	-27.783365558037527
11	22	-27.59937209167167
11	23	-27.4361026031681
11	24	-27.456549302170608
11	25	-26.67557910929451
11	26	-25.89334312497341
11	27	-26.06112843962021
11	28	-25.56681181159393
11	29	-25.05771055792103
11	30	-24.722475301199655
11	31	-24.065267751864308
11	32	-23.771102750047888
11	33	-23.74352374652637
11	34	-23.316781494404985
11	35	-23.679362733448833
11	36	-24.592774836876337
11	37	-23.906143123541653
11	38	-23.905805129963134
11	39	-23.62860345204716
11	40	-23.009164217766017
11	41	-23.506470778343825
11	42	-23.911994782784976
11	43	-23.32034393042296
11	44	-23.111233089309337
11	45	-22.5021645252679
11	46	-23.11751725294377
11	47	-22.287394912260442
11	48	-22.343140715768023
11	49	-22.39507018404181
11	50	-21.686739784069847
11	51	-21.161714290113526
11	52	-20.559460041572997
11	53	-20.405256841112852
11	54	-20.44740666302172
11	55	-19.921446644408118
11	56	-19.356769179691717
11	57	-19.190992312955764
11	58	-18.410717594928606
14	1	-1.5770254776446393
14	2	0.24987047276427177
14	3	-4.210053910101529
14	4	0.23330008140577263
14	5	-10.580229590801874
14	6	-4.843814583188646
14	7	-4.430323023603314
14	8	-0.7341387256191254
14	9	-3.7768189014006666
14	10	-1.2137421956084893
14	11	1.340723348163614
14	12	-6.611388226299965
14	13	2.4721777929673934
14	14	2.7318513336432773
14	15	2.5148612122600724
14	16	-13.240910885208361
14	17	-8.939333340883739
14	18	-8.195769702064204
14	19	2.1550165422629526
14	20	-15.308389107765201
14	21	-10.783365558037527
14	22	-13.599372091671668
14	23	-2.4361026031680986
14	24	-4.4565493021706075
14	25	-17.67557910929451
14	26	-10.89334312497341
14	27	2.9388715603797912
14	28	-6.566811811593929
14	29	-10.05771055792103
14	30	-5.722475301199655
14	31	-15.065267751864308
14	32	-7.771102750047888
14	33	-10.743523746526371
14	34	-6.316781494404985
14	35	3.3206372665511665
14	36	4.4072251631236625
14	37	-18.906143123541653
14	38	-7.9058051299631344
14	39	2.371396547952841
14	40	-6.009164217766017
14	41	-10.506470778343825
14	42	-1.9119947827849764
14	43	-10.320343930422961
14	44	-8.111233089309337
14	45	-7.502164525267901
14	46	-7.11751725294377
14	47	-1.2873949122604422
14	48	-1.3431407157680226
14	49	-1.3950701840418098
14	50	-5.686739784069847
14	51	-0.161714290113526
14	52	-4.559460041572997
14	53	-8.405256841112852
14	54	0.5525933369782798
14	55	-3.9214466444081175
14	56	3.6432308203082826
14	57	3.809007687044236
14	58	12.589282405071394
16	1	2.4229745223553607
16	2	4.249870472764272
16	3	-3.2100539101015286
16	4	4.233300081405773
16	5	-1.5802295908018742
16	6	3.156185416811354
16	7	-9.430323023603314
16	8	2.2658612743808746
16	9	3.2231810985993334
16	10	1.7862578043915107
16	11	4.340723348163614
16	12	-1.6113882262999653
16	13	5.472177792967393
16	14	5.731851333643277
16	15	5.514861212260072
16	16	1.7590891147916388
16	17	5.0606666591162615
16	18	-3.1957697020642044
16	19	-3.8449834577370474
16	20	-1.3083891077652012
16	21	8.216634441962473
16	22	6.400627908328332
16	23	8.563897396831901
16	24	2.5434506978293925
16	25	5.32442089070549
16	26	0.10665687502659082
16	27	1.9388715603797912
16	28	5.433188188406071
16	29	-17.05771055792103
16	30	3.2775246988003452
16	31	-5.065267751864308
16	32	-6.771102750047888
16	33	-14.743523746526371
16	34	-5.316781494404985
16	35	-7.6793627334488335
16	36	-7.5927748368763375
16	37	-6.9061431235416535
16	38	0.09419487003686555
16	39	2.371396547952841
16	40	-12.009164217766017
16	41	-2.506470778343825
16	42	-1.9119947827849764
16	43	-1.3203439304229612
16	44	-12.111233089309337
16	45	-1.5021645252679008
16	46	2.8824827470562298
16	47	7.712605087739558
16	48	7.656859284231977
16	49	7.60492981595819
16	50	-5.686739784069847
16	51	3.838285709886474
16	52	5.440539958427003
16	53	9.594743158887148
16	54	-20.44740666302172
16	55	-19.921446644408118
16	56	-19.356769179691717
16	57	-19.190992312955764
16	58	-18.410717594928606
18	1	-6.577025477644639
18	2	-21.75012952723573
18	3	-13.210053910101529
18	4	-21.766699918594227
18	5	-20.580229590801874
18	6	-10.843814583188646
18	7	-10.430323023603314
18	8	-10.734138725619125
18	9	-11.776818901400667
18	10	-10.21374219560849
18	11	0.34072334816361405
18	12	-1.6113882262999653
18	13	-1.5278222070326066
18	14	1.7318513336432773
18	15	1.5148612122600724
18	16	-0.24091088520836124
18	17	0.06066665911626146
18	18	-13.195769702064204
18	19	-13.844983457737047
18	20	3.6916108922347988
18	21	1.2166344419624728
18	22	1.4006279083283317
18	23	1.5638973968319014
18	24	1.5434506978293925
18	25	-26.67557910929451
18	26	-25.89334312497341
18	27	-26.06112843962021
18	28	-25.56681181159393
18	29	-25.05771055792103
18	30	-24.722475301199655
18	31	-24.065267751864308
18	32	-23.771102750047888
18	33	-23.74352374652637
18	34	-23.316781494404985
18	35	-23.679362733448833
18	36	-24.592774836876337
18	37	-23.906143123541653
18	38	-23.905805129963134
18	39	-23.62860345204716
18	40	-23.009164217766017
18	41	-23.506470778343825
18	42	-23.911994782784976
18	43	-23.32034393042296
18	44	-23.111233089309337
18	45	-22.5021645252679
18	46	-23.11751725294377
18	47	-22.287394912260442
18	48	-22.343140715768023
18	49	-22.39507018404181
18	50	-21.686739784069847
18	51	-21.161714290113526
18	52	-20.559460041572997
18	53	-20.405256841112852
18	54	-20.44740666302172
18	55	-19.921446644408118
18	56	-19.356769179691717
18	57	-19.190992312955764
18	58	-18.410717594928606
20	1	-1.5770254776446393
20	2	0.24987047276427177
20	3	-4.210053910101529
20	4	0.23330008140577263
20	5	-4.580229590801874
20	6	-6.843814583188646
20	7	-0.4303230236033144
20	8	0.2658612743808746
20	9	0.22318109859933344
20	10	-8.21374219560849
20	11	2.340723348163614
20	12	0.38861177370003475
20	13	-2.5278222070326066
20	14	0.7318513336432773
20	15	3.5148612122600724
20	16	1.7590891147916388
20	17	5.0606666591162615
20	18	-1.1957697020642044
20	19	5.155016542262953
20	20	5.691610892234799
20	21	8.216634441962473
20	22	-2.5993720916716683
20	23	8.563897396831901
20	24	8.543450697829392
20	25	6.32442089070549
20	26	5.106656875026591
20	27	4.938871560379791
20	28	-4.566811811593929
20	29	8.94228944207897
20	30	3.2775246988003452
20	31	-1.065267751864308
20	32	-0.7711027500478878
20	33	4.256476253473629
20	34	-0.31678149440498515
20	35	7.3206372665511665
20	36	-11.592774836876337
20	37	1.0938568764583465
20	38	-5.9058051299631344
20	39	1.3713965479528412
20	40	-2.009164217766017
20	41	4.493529221656175
20	42	4.088005217215024
20	43	4.679656069577039
20	44	4.888766910690663
20	45	9.4978354747321
20	46	12.88248274705623
20	47	9.712605087739558
20	48	6.656859284231977
20	49	10.60492981595819
20	50	14.313260215930153
20	51	-21.161714290113526
20	52	-20.559460041572997
20	53	-20.405256841112852
20	54	-20.44740666302172
20	55	-19.921446644408118
20	56	-19.356769179691717
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117	10	-10.21374219560849
117	11	-2.659276651836386
117	12	-15.611388226299965
117	13	-15.527822207032607
117	14	-1.2681486663567227
117	15	-1.4851387877399276
117	16	-29.24091088520836
117	17	-28.93933334088374
117	18	-28.195769702064204
117	19	-28.844983457737047
117	20	-28.3083891077652
117	21	-27.783365558037527
117	22	-27.59937209167167
117	23	-27.4361026031681
117	24	-27.456549302170608
117	25	-26.67557910929451
117	26	-25.89334312497341
117	27	-26.06112843962021
117	28	-25.56681181159393
117	29	-25.05771055792103
117	30	-24.722475301199655
117	31	-24.065267751864308
117	32	-23.771102750047888
117	33	-23.74352374652637
117	34	-23.316781494404985
117	35	-23.679362733448833
117	36	-24.592774836876337
117	37	-23.906143123541653
117	38	-23.905805129963134
117	39	-23.62860345204716
117	40	-23.009164217766017
117	41	-23.506470778343825
117	42	-23.911994782784976
117	43	-23.32034393042296
117	44	-23.111233089309337
117	45	-22.5021645252679
117	46	-23.11751725294377
117	47	-22.287394912260442
117	48	-22.343140715768023
117	49	-22.39507018404181
117	50	-21.686739784069847
117	51	-21.161714290113526
117	52	-20.559460041572997
117	53	-20.405256841112852
117	54	-20.44740666302172
117	55	-19.921446644408118
117	56	-19.356769179691717
117	57	-19.190992312955764
117	58	-18.410717594928606
119	1	-5.577025477644639
119	2	-23.75012952723573
119	3	-21.21005391010153
119	4	-21.766699918594227
119	5	-15.580229590801874
119	6	-16.843814583188646
119	7	-16.430323023603314
119	8	-17.734138725619125
119	9	-15.776818901400667
119	10	-18.21374219560849
119	11	-2.659276651836386
119	12	-1.6113882262999653
119	13	-1.5278222070326066
119	14	-1.2681486663567227
119	15	-1.4851387877399276
119	16	-0.24091088520836124
119	17	0.06066665911626146
119	18	0.8042302979357956
119	19	0.15501654226295258
119	20	-28.3083891077652
119	21	-27.783365558037527
119	22	-27.59937209167167
119	23	-27.4361026031681
119	24	-27.456549302170608
119	25	-26.67557910929451
119	26	-25.89334312497341
119	27	-26.06112843962021
119	28	-25.56681181159393
119	29	-25.05771055792103
119	30	-24.722475301199655
119	31	-24.065267751864308
119	32	-23.771102750047888
119	33	-23.74352374652637
119	34	-23.316781494404985
119	35	-23.679362733448833
119	36	-24.592774836876337
119	37	-23.906143123541653
119	38	-23.905805129963134
119	39	-23.62860345204716
119	40	-23.009164217766017
119	41	-23.506470778343825
119	42	-23.911994782784976
119	43	-23.32034393042296
119	44	-23.111233089309337
119	45	-22.5021645252679
119	46	-23.11751725294377
119	47	-22.287394912260442
119	48	-22.343140715768023
119	49	-22.39507018404181
119	50	-21.686739784069847
119	51	-21.161714290113526
119	52	-20.559460041572997
119	53	-20.405256841112852
119	54	-20.44740666302172
119	55	-19.921446644408118
119	56	-19.356769179691717
119	57	-19.190992312955764
119	58	-18.410717594928606
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	122.0
3	4.0
4	12.0
5	20.0
6	43.0
7	62.0
8	38.0
9	77.0
10	126.0
11	122.0
12	174.0
13	182.0
14	227.0
15	245.0
16	273.0
17	294.0
18	316.0
19	349.0
20	371.0
21	367.0
22	425.0
23	542.0
24	621.0
25	678.0
26	774.0
27	934.0
28	1114.0
29	1260.0
30	1497.0
31	1671.0
32	1952.0
33	2198.0
34	2742.0
35	3354.0
36	4277.0
37	5358.0
38	4871.0
39	2892.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	60.569175803868426	25.115190341259087	3.306640384378465	11.008993470494024
11	38.88382407293335	20.283355919674754	14.828138474805963	26.004681532585934
12	37.826783294320556	13.450782308734755	27.241591721079217	21.480842675865468
13	23.66391523962055	16.713071331772824	32.47505235924603	27.147961069360598
14	20.413946039177038	19.031661944068006	39.46531969939633	21.08907231735863
15	24.836762350622152	15.522976469138843	38.2407293334976	21.399531846741407
16	28.02266847357398	15.490944930393002	32.66231366268325	23.82407293334976
17	31.71615128742146	16.582481212270544	22.422077122089444	29.279290378218555
18	31.28988542564987	18.942959221387213	25.166933596156216	24.600221756806704
19	27.756560305531604	22.446716767278552	27.926573857336457	21.870149069853394
20	29.367993100899348	22.91487002587163	25.415794012566217	22.301342860662807
21	31.022077666075297	21.077271831263552	26.320717524147447	21.5799329785137
22	34.32795367746704	18.8049772083282	25.381298509301466	21.48577060490329
23	34.842922261919426	20.574103732906245	23.395343107059258	21.187630898115067
24	32.91856597264999	23.952199088333128	21.83811753110755	21.291117407909326
25	30.048047308118765	20.79832450412714	20.142909942096836	29.010718245657262
26	29.1879235982748	18.58287122612446	22.06531115218731	30.163894023413434
27	24.023654059381546	19.386472834791178	22.587162744856474	34.0027103609708
28	24.628465804066543	25.165742452248924	22.528650646950094	27.67714109673444
29	30.80486963379171	19.685543890778252	24.759722016856426	24.74986445857361
30	27.46388601291722	22.208253216979738	25.654488980919982	24.67337178918306
31	31.68248490077653	18.83150499198817	26.614076174041664	22.871933933193638
32	38.45130126182965	14.994085173501578	26.717764195583598	19.836849369085176
33	36.595943914639854	15.965599664867794	25.775609275276608	21.662847145215743
34	30.44485520640789	21.454097350585336	29.831176833025264	18.269870609981513
35	26.986395899053626	27.42754337539432	30.47614353312303	15.109917192429023
36	27.211320382605265	30.75880090720836	25.547283305393947	16.482595404792427
37	29.54679948712891	26.671762501232866	24.21836473024953	19.563073281388697
38	28.525980911983034	27.37182174652889	24.338454709117364	19.76374263237071
39	29.832823749876713	25.42657066771871	24.154255843771573	20.586349738633
40	28.505560075942498	22.797544197055995	26.17550607786572	22.521389649135784
41	26.111618042368494	23.946336531110507	27.10547732373178	22.83656810278922
42	25.17328991835023	22.30444756900762	27.038160783443104	25.484101729199043
43	24.658378965023925	20.43066449607814	26.92763060529821	27.983325933599723
44	27.58033886600735	18.12464547315461	27.56307495005796	26.731940710780083
45	28.49956848723955	20.811243989643692	26.265565281716185	24.423622241400565
46	29.75065972821664	22.904283917429154	24.647939428317756	22.69711692603645
47	24.49548527162382	22.958503971974146	28.494597128336707	24.051413628065326
48	26.414209942025412	21.887257925249784	27.963488343406933	23.73504378931787
49	26.287476866132014	20.31832202344232	28.967304133251083	24.426896977174582
50	22.08978060758619	19.372670960736407	30.59154512475013	27.94600330692727
51	21.710704857770214	20.346384427503516	32.06523079959539	25.87767991513088
52	21.766374737880845	19.861847785864068	35.48044899469594	22.89132848155915
53	19.737458977965307	20.26550201100501	34.312927184346236	25.68411182668345
54	22.673313754041313	21.333201707840765	32.65381672795479	23.339667810163135
55	22.661630748722505	20.444839418400846	30.203164728825694	26.69036510405095
56	22.843103363129043	23.07027507531236	25.181490443972542	28.905131117586052
57	25.977136367003283	23.177205501098737	24.36976864768771	26.47588948421027
58	24.886397313049493	26.65958707892917	26.664526326187886	21.78948928183345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	12.5
10	25.0
11	35.5
12	46.0
13	44.0
14	42.0
15	40.0
16	74.5
17	111.0
18	141.0
19	171.0
20	178.0
21	185.0
22	210.0
23	264.5
24	294.0
25	367.5
26	441.0
27	492.0
28	592.5
29	642.0
30	713.5
31	785.0
32	681.0
33	577.0
34	735.5
35	698.5
36	503.0
37	485.0
38	467.0
39	533.5
40	600.0
41	518.5
42	587.5
43	738.0
44	1079.0
45	1420.0
46	1362.5
47	1100.5
48	896.0
49	957.5
50	1019.0
51	1122.5
52	1226.0
53	1601.5
54	1966.0
55	1955.0
56	1963.0
57	1971.0
58	2383.0
59	3215.0
60	3635.0
61	3552.0
62	3469.0
63	3392.5
64	3316.0
65	2887.5
66	2125.0
67	1791.0
68	1418.5
69	1046.0
70	951.5
71	857.0
72	753.5
73	536.0
74	422.0
75	347.0
76	272.0
77	209.5
78	112.0
79	77.0
80	66.5
81	56.0
82	36.5
83	17.0
84	16.5
85	12.0
86	8.0
87	7.0
88	6.0
89	4.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0024639645189109276
22	0.0
23	0.0
24	0.0
25	0.0
26	0.024639645189109275
27	0.0
28	0.024639645189109275
29	0.017247751632376494
30	0.04681532585930763
31	0.04927929037821855
32	0.022175680670198348
33	0.00985585807564371
34	0.024639645189109275
35	0.022175680670198348
36	0.051743254897129484
37	0.0714549710484169
38	0.08870272268079339
39	0.0714549710484169
40	0.06899100652950597
41	0.08870272268079339
42	0.11334236786990268
43	0.1059504743131699
44	0.09363065171861526
45	0.07391893556732783
46	0.09363065171861526
47	0.1256621904644573
48	0.12319822594554639
49	0.14783787113465566
50	0.15769372921029937
51	0.12812615498336824
52	0.12319822594554639
53	0.1429099420968338
54	0.16262165824812125
55	0.18726130343723052
56	0.2143649131452507
57	0.20697301958851794
58	0.22914870025871628
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	40585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.51533817913022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63932577601044	30.156461746950843
2	11.39533503472016	9.461623752617962
3	5.1516410469464065	6.416163607244056
4	2.9912754466140425	4.96735247012443
5	1.7330405365303578	3.5973881976099547
6	1.3116505430589354	3.26721695207589
7	0.7596890023146775	2.207712208944191
8	0.611312244050092	2.0303067635826046
9	0.4866757671078402	1.8184058149562647
>10	2.5936257344649536	20.298139706788223
>50	0.23740281322333667	6.470370826660096
>100	0.08309098462816784	8.017740544536158
>500	0.005935070330583418	1.2911174079093262
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTGGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	524	1.2911174079093262	No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	376	0.9264506591105087	No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	322	0.7933965750893187	No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	309	0.7613650363434766	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	307	0.7564371073056548	No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	302	0.7441172847111002	No Hit
TTGGACGGGAAGAGAGAAATTGTATCCCCGTGCATACTATTTAGTGCATTCCCATGCA	291	0.7170136750030799	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	253	0.6233830232844647	No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	200	0.49279290378218554	No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	191	0.4706172231119872	No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	181	0.4459775779228779	No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	160	0.3942343230257484	No Hit
TTGGACGGGATCTTATGAATGCTACAAATATGTAATGTTGGAAGAGTATATTATTTGT	137	0.3375631390907971	No Hit
TTGGACGGGAGGTATTTGTAGTTTACCCTAATGCTTCTATAGAAGGAAGTAGGAACAA	124	0.305531600344955	No Hit
TTGGACGGGGGAGCAGGCAGCCTAGGCCTGGGACTGGAACAGCGCGGAGATGGGGGCG	101	0.2488604164100037	No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	93	0.22914870025871628	No Hit
TTGGACGGGGGGCGTCGGCTGTCGGAGGAGCTAAGTCCTGACATGTCTCCAGCTGGGG	92	0.22668473573980535	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	89	0.21929284218307254	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	84	0.20697301958851794	No Hit
TTGGACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	82	0.20204509055069605	No Hit
TTGGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	80	0.1971171615128742	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	80	0.1971171615128742	No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC	74	0.18233337439940864	No Hit
TTGGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	72	0.17740544536158678	No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70	0.17247751632376493	No Hit
TTGGACGGGCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGGCA	69	0.170013551804854	No Hit
TTGGACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	69	0.170013551804854	No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	69	0.170013551804854	No Hit
TTGGACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	68	0.16754958728594307	No Hit
TTGGACGGGAGCCCAAAACAATACAGAAAATCAGTCACCTTTGCTGTCTATCAGTGGC	68	0.16754958728594307	No Hit
TTGGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	66	0.16262165824812125	No Hit
TTGGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	66	0.16262165824812125	No Hit
TTGGACGGGTGCTTCAGGGCTTGCCTAGTGTCCTCTCCTCATTGGTAGCTCTCACTAG	66	0.16262165824812125	No Hit
TTGGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	65	0.1601576937292103	No Hit
TTGGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	65	0.1601576937292103	No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	65	0.1601576937292103	No Hit
TTGGACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	64	0.15769372921029937	No Hit
TTGGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	64	0.15769372921029937	No Hit
TTGGACGGGACGCCAACTTGAAAATGATTTGCAAACCCAGAAAAGGTCACACACTGAC	64	0.15769372921029937	No Hit
TTGGACGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAA	62	0.1527658001724775	No Hit
TTGGACGGGGAGAGGGGGGAAAAACAAGTAAAAATAAATCGTGTTGTTGAATTTAAAA	62	0.1527658001724775	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	60	0.14783787113465566	No Hit
TTGGACGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA	59	0.14537390661574473	No Hit
TTGGACGGGATGCAGTGCTTTGGACGGGAGTTCGTCCCGAATAAGTTCCTGTTACCAG	58	0.1429099420968338	No Hit
TTGGACGGGAGAATAACATTAGCCAGTTTAGGTTCCTGTTTTTTCCTTGTTTGAGACA	56	0.13798201305901195	No Hit
TTGGACGGGAGCTACCTGAGATCTTGGTGCCTTCAAACTAGGCAGCCATGGAATAAGG	54	0.1330540840211901	No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.13059011950227917	No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATACTGAAAAACATCGAAC	53	0.13059011950227917	No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG	53	0.13059011950227917	No Hit
TTGGACGGGGGCAGTAGGGTCGGTCGGCCCTGAGCAGGCAGCCTAGGCCTGGGACTGG	53	0.13059011950227917	No Hit
TTGGACGGGACCAAGGACTAAGCATTCAAATTTATGAGCCGATAGGGGCCATTCTCAT	52	0.12812615498336824	No Hit
TTGGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA	52	0.12812615498336824	No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT	52	0.12812615498336824	No Hit
TTGGACGGGGAGGAGGGGTGGGGTCCTGAACATGGCGGACCTCAGCTTTTCTGACGGC	52	0.12812615498336824	No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	51	0.1256621904644573	No Hit
TTGGACGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG	50	0.12319822594554639	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	49	0.12073426142663544	No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG	49	0.12073426142663544	No Hit
TTGGACGGGAGCAGTGTGTTCGCAGGTTTTTAGACGTCTTGGTGTTTCTAATATTAAC	49	0.12073426142663544	No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	48	0.11827029690772452	No Hit
TTGGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	47	0.11580633238881359	No Hit
TTGGACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	47	0.11580633238881359	No Hit
TTGGACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC	46	0.11334236786990268	No Hit
TTGGACGGGGGGAAAAAGGGGTAAATTCGAATCAAAAACATTTTTTAGGATATTTCTT	46	0.11334236786990268	No Hit
TTGGACGGGAACCAGGAAACTGAACTCTTTCCTGGATTGTGAGTGCTTCGAAGGGACT	46	0.11334236786990268	No Hit
TTGGACGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA	45	0.11087840335099175	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA	45	0.11087840335099175	No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	45	0.11087840335099175	No Hit
TTGGACGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC	45	0.11087840335099175	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA	44	0.10841443883208082	No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA	44	0.10841443883208082	No Hit
TTGGACGGGGAACCAAATATTCAATAGTGAGCTGGTGTAGGTTATGTACACAGTGCTT	44	0.10841443883208082	No Hit
TTGGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA	44	0.10841443883208082	No Hit
TTGGACGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	44	0.10841443883208082	No Hit
TTGGACGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT	42	0.10348650979425897	No Hit
TTGGACGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAA	42	0.10348650979425897	No Hit
TTGGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG	42	0.10348650979425897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0024639645189109276	0.0	0.0	0.0	0.0
11	0.0024639645189109276	0.0	0.0	0.0	0.0
12	0.0024639645189109276	0.0	0.0	0.0	0.0
13	0.004927929037821855	0.0	0.0	0.0	0.0
14	0.017247751632376494	0.0	0.0	0.0	0.0
15	0.017247751632376494	0.0	0.0	0.0	0.0
16	0.017247751632376494	0.0	0.0	0.0	0.0
17	0.017247751632376494	0.0	0.0	0.0	0.0
18	0.01971171615128742	0.0	0.0	0.0	0.0
19	0.01971171615128742	0.0	0.0	0.0	0.0
20	0.027103609708020206	0.0	0.0	0.0	0.0
21	0.027103609708020206	0.0	0.0	0.0	0.0
22	0.027103609708020206	0.0	0.0	0.0	0.0
23	0.03942343230257484	0.0	0.0	0.0	0.0
24	0.044351361340396696	0.0	0.0	0.0	0.0
25	0.044351361340396696	0.0	0.0	0.0	0.0
26	0.04681532585930762	0.0	0.0	0.0	0.0
27	0.04681532585930762	0.0	0.0	0.0	0.0
28	0.06159911297277319	0.0	0.0	0.0	0.0
29	0.06159911297277319	0.0	0.0	0.0	0.0
30	0.08131082912406061	0.0	0.0	0.0	0.0
31	0.08377479364297154	0.0	0.0	0.0	0.0
32	0.08623875816188246	0.0	0.0	0.0	0.0
33	0.08623875816188246	0.0	0.0	0.0	0.0
34	0.08623875816188246	0.0	0.0	0.0	0.0
35	0.10841443883208082	0.0	0.0	0.0	0.0
36	0.10841443883208082	0.0	0.0	0.0	0.0
37	0.10841443883208082	0.0	0.0	0.0	0.0
38	0.10841443883208082	0.0	0.0	0.0	0.0
39	0.11334236786990268	0.0	0.0	0.0	0.0
40	0.11334236786990268	0.0	0.0	0.0	0.0
41	0.1158063323888136	0.0	0.0	0.0	0.0
42	0.11827029690772453	0.0	0.0	0.0	0.0
43	0.12073426142663546	0.0	0.0	0.0	0.0
44	0.12319822594554639	0.0	0.0	0.0	0.0
45	0.12319822594554639	0.0	0.0	0.0	0.0
46	0.12812615498336824	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCATG	60	0.0	52.084057	50
TTATTTG	15	0.0071244994	52.084057	51
TCGAACT	60	0.0	52.084057	52
CCATGCA	60	0.0	52.084057	52
ATGAAGC	15	0.0071244994	52.084057	52
AGGAACA	15	0.0071244994	52.084057	51
AACTTTG	15	0.0071244994	52.084057	50
TTCCAAA	25	1.6112606E-5	52.084057	52
TCTGAAG	15	0.0071244994	52.084057	51
TATTTCT	15	0.0071244994	52.084057	51
ATATGGG	15	0.0071244994	52.084057	52
ATGTTTT	15	0.0071244994	52.084057	51
AGATGAA	15	0.0071244994	52.084057	50
ATATTTC	15	0.0071244994	52.084057	50
TGTTACC	20	3.380943E-4	52.084053	50
ACAGCGC	20	3.401674E-4	52.019753	39
ACAAATA	15	0.00715945	52.019753	24
ATCCCCG	60	0.0	52.019753	24
TAGAACT	15	0.00715945	52.019753	47
CTACAAA	15	0.00715945	52.019753	22
>>END_MODULE