FastQCFastQC Report
Tue 31 May 2016
SRR212506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43184
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5301.2273064097814006No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4200.9725824379399778No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3270.7572248981104113No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3150.7294368284549834No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3140.7271211559836976No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3130.724805483512412No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2940.6808077065579845No Hit
TTGGACGGGAAGAGAGAAATTGTATCCCCGTGCATACTATTTAGTGCATTCCCATGCA2850.6599666543164134No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2130.49323823638384584No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2040.4723971841422749No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1850.42839940718784736No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1750.4052426824749908No Hit
TTGGACGGGAGGTATTTGTAGTTTACCCTAATGCTTCTATAGAAGGAAGTAGGAACAA1540.3566135605779919No Hit
TTGGACGGGATCTTATGAATGCTACAAATATGTAATGTTGGAAGAGTATATTATTTGT1250.28945905891070767No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1170.2709336791404224No Hit
TTGGACGGGGGAGCAGGCAGCCTAGGCCTGGGACTGGAACAGCGCGGAGATGGGGGCG1130.26167098925527976No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1030.23851426454242314No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA900.20841052241570954No Hit
TTGGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT830.1922008151167099No Hit
TTGGACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT810.18756947017413858No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA800.1852537977028529No Hit
TTGGACGGGGGGCGTCGGCTGTCGGAGGAGCTAAGTCCTGACATGTCTCCAGCTGGGG780.18062245276028158No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC730.16904409040385326No Hit
TTGGACGGGAGAATAACATTAGCCAGTTTAGGTTCCTGTTTTTTCCTTGTTTGAGACA730.16904409040385326No Hit
TTGGACGGGGAGAGGGGGGAAAAACAAGTAAAAATAAATCGTGTTGTTGAATTTAAAA730.16904409040385326No Hit
TTGGACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT710.16441274546128196No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA700.1620970729899963No Hit
TTGGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT670.1551500555761393No Hit
TTGGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC670.1551500555761393No Hit
TTGGACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC660.15283438310485364No Hit
TTGGACGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAA660.15283438310485364No Hit
TTGGACGGGATGCAGTGCTTTGGACGGGAGTTCGTCCCGAATAAGTTCCTGTTACCAG660.15283438310485364No Hit
TTGGACGGGAACCAGGAAACTGAACTCTTTCCTGGATTGTGAGTGCTTCGAAGGGACT640.14820303816228234No Hit
TTGGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA630.14588736569099667No Hit
TTGGACGGGGCTTCCTCCTCGTGGCGGCCCGGGAGCTAGGAGCACCGGTCTGCGCCGG630.14588736569099667No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC630.14588736569099667No Hit
TTGGACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT630.14588736569099667No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA610.14125602074842536No Hit
TTGGACGGGACGCCAACTTGAAAATGATTTGCAAACCCAGAAAAGGTCACACACTGAC610.14125602074842536No Hit
TTGGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG600.1389403482771397No Hit
TTGGACGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA600.1389403482771397No Hit
TTGGACGGGCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGGCA590.13662467580585402No Hit
TTGGACGGGAGCCCAAAACAATACAGAAAATCAGTCACCTTTGCTGTCTATCAGTGGC590.13662467580585402No Hit
TTGGACGGGTGCTTCAGGGCTTGCCTAGTGTCCTCTCCTCATTGGTAGCTCTCACTAG590.13662467580585402No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA580.13430900333456836No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG570.1319933308632827No Hit
TTGGACGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG560.12967765839199705No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA560.12967765839199705No Hit
TTGGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT550.12736198592071138No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.12736198592071138No Hit
TTGGACGGGAGCTACCTGAGATCTTGGTGCCTTCAAACTAGGCAGCCATGGAATAAGG540.1250463134494257No Hit
TTGGACGGGGGGAAAAAGGGGTAAATTCGAATCAAAAACATTTTTTAGGATATTTCTT540.1250463134494257No Hit
TTGGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG540.1250463134494257No Hit
TTGGACGGGGGCAGTAGGGTCGGTCGGCCCTGAGCAGGCAGCCTAGGCCTGGGACTGG540.1250463134494257No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.12273064097814004No Hit
TTGGACGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA520.12041496850685439No Hit
TTGGACGGGGTGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA520.12041496850685439No Hit
TTGGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA510.11809929603556872No Hit
TTGGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG500.11578362356428308No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA480.11115227862171174No Hit
TTGGACGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCACGC480.11115227862171174No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT470.10883660615042608No Hit
TTGGACGGGGAAGAAAGCGATGCCACGTGATCTCAGCACCATCCAGTTAAAAGGAGGT470.10883660615042608No Hit
TTGGACGGGGAGGAGGGGTGGGGTCCTGAACATGGCGGACCTCAGCTTTTCTGACGGC470.10883660615042608No Hit
TTGGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT460.10652093367914041No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG460.10652093367914041No Hit
TTGGACGGGAGCAGTGTGTTCGCAGGTTTTTAGACGTCTTGGTGTTTCTAATATTAAC460.10652093367914041No Hit
TTGGACGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG460.10652093367914041No Hit
TTGGACGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC450.10420526120785477No Hit
TTGGACGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA440.1018895887365691No Hit
TTGGACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC440.1018895887365691No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT440.1018895887365691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGC150.00715626652.03132231
ACAGTGC150.00715626652.03132250
ACCGGTC203.4004016E-452.03132244
ACAGCAT150.00715626652.03132233
TGTTGGA150.00715626652.03132236
TGCATTC307.783128E-752.03132245
TGCATAC307.783128E-752.03132231
GTGCATT307.783128E-752.03132244
GTGCATA307.783128E-752.03132230
CAGCATG150.00715626652.03132234
TATATTA150.00715626652.03132247
GTCATCC150.00715626652.03132250
CGGAGAT150.00715626652.03132245
TAGTGCA307.783128E-752.03132242
TTCGTCC150.00715626652.03132231
ACTGACG150.00715626652.03132245
CCTGTTA150.00715626652.03132248
TTACCAG150.00715626652.03132252
TGACTAC150.00715626652.03132243
GACAGCA150.00715626652.03132232
Produced by FastQC (version 0.11.5)