FastQCFastQC Report
Tue 31 May 2016
SRR212505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44901
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5261.171466114340438No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3800.8463063183448031No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3450.7683570521814659No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3180.7082247611411773No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2970.661455201443175No Hit
TTGGACGGGAAGAGAGAAATTGTATCCCCGTGCATACTATTTAGTGCATTCCCATGCA2790.6213670074163159No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC2720.6057771541836484No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2380.5300550099106924No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2140.47660408454154696No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1990.4431972561858311No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1860.41424467161087725No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1590.35411238057058864No Hit
TTGGACGGGATCTTATGAATGCTACAAATATGTAATGTTGGAAGAGTATATTATTTGT1480.32961403977639697No Hit
TTGGACGGGAGGTATTTGTAGTTTACCCTAATGCTTCTATAGAAGGAAGTAGGAACAA1210.2694817487361083No Hit
TTGGACGGGGGAGCAGGCAGCCTAGGCCTGGGACTGGAACAGCGCGGAGATGGGGGCG1190.2650275049553462No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1040.23162067659963032No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1030.22939355470924924No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC990.220485067147725No Hit
TTGGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT880.19598672635353334No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC870.19375960446315227No Hit
TTGGACGGGGAGAGGGGGGAAAAACAAGTAAAAATAAATCGTGTTGTTGAATTTAAAA860.1915324825727712No Hit
TTGGACGGGGGGCGTCGGCTGTCGGAGGAGCTAAGTCCTGACATGTCTCCAGCTGGGG830.18485111690162803No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG810.1803968731208659No Hit
TTGGACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT780.17371550744972272No Hit
TTGGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG770.17148838555934165No Hit
TTGGACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT760.1692612636689606No Hit
TTGGACGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAA760.1692612636689606No Hit
TTGGACGGGAGCCCAAAACAATACAGAAAATCAGTCACCTTTGCTGTCTATCAGTGGC740.16480701988819849No Hit
TTGGACGGGCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGGCA730.1625798979978174No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA690.15367141043629318No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC670.14921716665553106No Hit
TTGGACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT660.14699004476514999No Hit
TTGGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT640.14253580098438787No Hit
TTGGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC640.14253580098438787No Hit
TTGGACGGGAGCTACCTGAGATCTTGGTGCCTTCAAACTAGGCAGCCATGGAATAAGG640.14253580098438787No Hit
TTGGACGGGACGCCAACTTGAAAATGATTTGCAAACCCAGAAAAGGTCACACACTGAC640.14253580098438787No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC630.14030867909400682No Hit
TTGGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA600.13362731342286363No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA600.13362731342286363No Hit
TTGGACGGGAACCAGGAAACTGAACTCTTTCCTGGATTGTGAGTGCTTCGAAGGGACT600.13362731342286363No Hit
TTGGACGGGAGAATAACATTAGCCAGTTTAGGTTCCTGTTTTTTCCTTGTTTGAGACA590.13140019153248256No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.13140019153248256No Hit
TTGGACGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA580.1291730696421015No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA580.1291730696421015No Hit
TTGGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG570.12694594775172044No Hit
TTGGACGGGATGCAGTGCTTTGGACGGGAGTTCGTCCCGAATAAGTTCCTGTTACCAG560.1247188258613394No Hit
TTGGACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC550.12249170397095833No Hit
TTGGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT550.12249170397095833No Hit
TTGGACGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA530.11803746019019622No Hit
TTGGACGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT520.11581033829981516No Hit
TTGGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT520.11581033829981516No Hit
TTGGACGGGGGCAGTAGGGTCGGTCGGCCCTGAGCAGGCAGCCTAGGCCTGGGACTGG520.11581033829981516No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATACTGAAAAACATCGAAC500.11135609451905303No Hit
TTGGACGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG480.10690185073829091No Hit
TTGGACGGGGCTTCCTCCTCGTGGCGGCCCGGGAGCTAGGAGCACCGGTCTGCGCCGG480.10690185073829091No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA470.10467472884790985No Hit
TTGGACGGGGATACAAAAGTTTCTTGAAGGTTCTTCATAATTGCGGTGTAGAAGAAAT470.10467472884790985No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA460.10244760695752879No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA460.10244760695752879No Hit
TTGGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA450.10022048506714773No Hit
TTGGACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC450.10022048506714773No Hit
TTGGACGGGTGCTTCAGGGCTTGCCTAGTGTCCTCTCCTCATTGGTAGCTCTCACTAG450.10022048506714773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT401.7826096E-952.1420644
TAGTGCA401.7826096E-952.1420642
TGTCTCA150.00709807752.1420641
AACCTTA150.00709807752.1420638
AAGGGAC203.3662602E-452.1420651
AGGGACT203.3662602E-452.1420652
AACTCGC150.00709807752.1420638
AAAAGGT150.00709807752.1420641
GCAAAGG150.00709807752.1420652
GCTAAAT150.00709807752.1420652
TGCTTCG203.3662602E-452.1420644
ATTAAGC150.00709807752.1420651
AAAGGTC150.00709807752.1420642
GTCTCAA150.00709807752.1420642
TATTTAG458.54925E-1152.1420638
GACGAAC150.00709807752.1420651
AGGTCAC150.00709807752.1420644
CTAAAGC203.3662602E-452.1420643
ACCTTAA150.00709807752.1420639
AGCCGTC150.00709807752.1420639
Produced by FastQC (version 0.11.5)