FastQCFastQC Report
Tue 31 May 2016
SRR212504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125883
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC16381.3012082648173304No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT15331.2177974786110912No Hit
TTGGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC14481.1502744612060405No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12791.0160228148359984No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC12611.001723822914929No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10630.8444349117831638No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9770.7761175059380535No Hit
TTGGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA9610.7634072908971029No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8660.6879403890914579No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8390.6664919012098536No Hit
TTGGACGGGAAGAGAGAAATTGTATCCCCGTGCATACTATTTAGTGCATTCCCATG6480.5147637091585043No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6010.47742745247571156No Hit
TTGGACGGGAGGTATTTGTAGTTTACCCTAATGCTTCTATAGAAGGAAGTAGGAAC4850.3852783934288188No Hit
TTGGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4560.3622411286670956No Hit
TTGGACGGGATCTTATGAATGCTACAAATATGTAATGTTGGAAGAGTATATTATTT4120.32728803730448114No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4060.3225217066641246No Hit
TTGGACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4050.3217273182240652No Hit
TTGGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3670.29154055750180724No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3460.2748584002605594No Hit
TTGGACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3320.2637369620997275No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3310.2629425736596681No Hit
TTGGACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3100.24626041641842028No Hit
TTGGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3050.2422884742181232No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2940.23355020137746954No Hit
TTGGACGGGGAGAGGGGGGAAAAACAAGTAAAAATAAATCGTGTTGTTGAATTTAA2820.2240175400967565No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2660.2113073250558058No Hit
TTGGACGGGCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGG2470.19621394469467682No Hit
TTGGACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2460.19541955625461738No Hit
TTGGACGGGGGAGCAGGCAGCCTAGGCCTGGGACTGGAACAGCGCGGAGATGGGGG2360.1874756718540232No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2360.1874756718540232No Hit
TTGGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2110.1676159608525377No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.16523279553235942No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA1970.1564945226917058No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1970.1564945226917058No Hit
TTGGACGGGATGCAGTGCTTTGGACGGGAGTTCGTCCCGAATAAGTTCCTGTTACC1960.15570013425164636No Hit
TTGGACGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCA1960.15570013425164636No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1900.15093380361128986No Hit
TTGGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1840.14616747297093333No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1810.14378430765075506No Hit
TTGGACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1790.14219553077063624No Hit
TTGGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1770.14060675389051738No Hit
TTGGACGGGACGCCAACTTGAAAATGATTTGCAAACCCAGAAAAGGTCACACACTG1720.1366348116902203No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1680.1334572579299826No Hit
TTGGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1630.12948531572968552No Hit
TTGGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT1600.12710215040950723No Hit
TTGGACGGGAGAATAACATTAGCCAGTTTAGGTTCCTGTTTTTTCCTTGTTTGAGA1590.12630776196944782No Hit
TTGGACGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA1580.1255133735293884No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1570.12471898508932898No Hit
TTGGACGGGATTTTATTTAAGCCTTTTAGTCCCACAAAAGTAGTTCCTCAATGTAT1570.12471898508932898No Hit
TTGGACGGGGAAGAAAGCGATGCCACGTGATCTCAGCACCATCCAGTTAAAAGGAG1570.12471898508932898No Hit
TTGGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1540.12233581976915071No Hit
TTGGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC1520.12074704288903189No Hit
TTGGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1510.11995265444897246No Hit
TTGGACGGGGGCAGTAGGGTCGGTCGGCCCTGAGCAGGCAGCCTAGGCCTGGGACT1490.11836387756885362No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC1480.1175694891287942No Hit
TTGGACGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC1450.11518632380861595No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1440.11439193536855652No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.11439193536855652No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1420.1128031584884377No Hit
TTGGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA1370.10883121628814058No Hit
TTGGACGGGACCAAGGACTAAGCATTCAAATTTATGAGCCGATAGGGGCCATTCTC1350.10724243940802175No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1340.10644805096796232No Hit
TTGGACGGGGATACAAAAGTTTCTTGAAGGTTCTTCATAATTGCGGTGTAGAAGAA1340.10644805096796232No Hit
TTGGACGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCG1340.10644805096796232No Hit
TTGGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1330.1056536625279029No Hit
TTGGACGGGAGTCGAAACTGATGCACCATAGGATTTCTGGCCTGGGGCAGGTATAA1330.1056536625279029No Hit
TTGGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1310.10406488564778406No Hit
TTGGACGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1310.10406488564778406No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1290.1024761087676652No Hit
TTGGACGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1290.1024761087676652No Hit
TTGGACGGGGAGGAGGGGTGGGGTCCTGAACATGGCGGACCTCAGCTTTTCTGACG1280.1016817203276058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA150.00820927850.311850
CCCCTAT150.00820927850.311850
TCGCAAA150.00820927850.311850
CAACAGT354.9882146E-850.311850
CACGACT252.0017009E-550.311850
AATATTA150.00820927850.311850
TCCCATG700.050.311850
ACCTTAT150.00820927850.311850
GTAGGAA650.050.2916249
GTGTTGA150.00822231450.29161548
TAATATT150.00822231450.29161549
ACACGAC150.00822231450.29161549
CACCTTA150.00822231450.29161549
TGTTGAC150.00822231450.29161549
TTCCCAT700.050.29161549
TCATGCT150.00822231450.29161548
ATATTAT451.2732926E-1050.29161548
TATATTA451.2732926E-1050.25130547
ACTATTA204.067587E-450.251347
AGCATAC150.00824843250.251347
Produced by FastQC (version 0.11.5)