FastQCFastQC Report
Tue 31 May 2016
SRR212503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118633
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC18081.524027884315494No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC17161.446477792856962No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT13621.1480785278969596No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13451.1337486196926656No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12281.0351251338160545No Hit
TTGGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA10060.8479933913835105No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9670.8151188960913068No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9520.8024748594404592No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9310.7847732081292725No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7810.658332841620797No Hit
TTGGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA6220.5243060531218127No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA5410.4560282552072358No Hit
TTGGACGGGAAGAGAGAAATTGTATCCCCGTGCATACTATTTAGTGCATTCCCATG5340.45012770477017355No Hit
TTGGACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4780.402923301273676No Hit
TTGGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4660.3928080719529979No Hit
TTGGACGGGAGGTATTTGTAGTTTACCCTAATGCTTCTATAGAAGGAAGTAGGAAC3920.3304308244754832No Hit
TTGGACGGGATCTTATGAATGCTACAAATATGTAATGTTGGAAGAGTATATTATTT3860.32537320981514417No Hit
TTGGACGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3750.3161009162711893No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3560.30008513651344904No Hit
TTGGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3270.2756399989884771No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3170.2672106412212453No Hit
TTGGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3110.26215302656090633No Hit
TTGGACGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3090.26046715500746No Hit
TTGGACGGGGAGAGGGGGGAAAAACAAGTAAAAATAAATCGTGTTGTTGAATTTAA2560.2157915588411319No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2510.21157687995751606No Hit
TTGGACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2430.20483339374373064No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2330.19640403597649894No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2090.17617357733514283No Hit
TTGGACGGGGAAGAAAGCGATGCCACGTGATCTCAGCACCATCCAGTTAAAAGGAG2090.17617357733514283No Hit
TTGGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC2080.17533064155841965No Hit
TTGGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2080.17533064155841965No Hit
TTGGACGGGGGAGCAGGCAGCCTAGGCCTGGGACTGGAACAGCGCGGAGATGGGGG2010.16943009112135746No Hit
TTGGACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA1970.16605834801446478No Hit
TTGGACGGGCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGG1890.1593148618006794No Hit
TTGGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1870.15762899024723306No Hit
TTGGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1840.15510018291706357No Hit
TTGGACGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA1810.15257137558689404No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1800.15172843981017087No Hit
TTGGACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1790.1508855040334477No Hit
TTGGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1770.14919963248000134No Hit
TTGGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1740.14667082514983185No Hit
TTGGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1710.14414201781966232No Hit
TTGGACGGGACGCCAACTTGAAAATGATTTGCAAACCCAGAAAAGGTCACACACTG1700.14329908204293915No Hit
TTGGACGGGATGCAGTGCTTTGGACGGGAGTTCGTCCCGAATAAGTTCCTGTTACC1690.142456146266216No Hit
TTGGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA1610.1357126600524306No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1600.13486972427570743No Hit
TTGGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTC1580.1331838527222611No Hit
TTGGACGGGAGAATAACATTAGCCAGTTTAGGTTCCTGTTTTTTCCTTGTTTGAGA1570.13234091694553793No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1560.13149798116881475No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.13065504539209158No Hit
TTGGACGGGGACTTTTACCTATTTTTATGTAATGTATACAGGTTATACAGTGCTAA1520.12812623806192205No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.12728330228519888No Hit
TTGGACGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCA1430.12053981607141352No Hit
TTGGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1400.118011008741244No Hit
TTGGACGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC1370.11548220141107449No Hit
TTGGACGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC1370.11548220141107449No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC1360.11463926563435131No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1330.11211045830418181No Hit
TTGGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT1320.11126752252745864No Hit
TTGGACGGGAGCCCAAAACAATACAGAAAATCAGTCACCTTTGCTGTCTATCAGTG1320.11126752252745864No Hit
TTGGACGGGGGCAGTAGGGTCGGTCGGCCCTGAGCAGGCAGCCTAGGCCTGGGACT1280.10789577942056595No Hit
TTGGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1260.1062099078671196No Hit
TTGGACGGGCGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA1250.10536697209039643No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGC1230.1036811005369501No Hit
TTGGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1220.10283816476022692No Hit
TTGGACGGGAGTCGAAACTGATGCACCATAGGATTTCTGGCCTGGGGCAGGTATAA1220.10283816476022692No Hit
TTGGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1210.10199522898350374No Hit
TTGGACGGGACCAAGGACTAAGCATTCAAATTTATGAGCCGATAGGGGCCATTCTC1200.10115229320678057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG204.078291E-450.2221850
CCCCTAT402.5447662E-950.2221850
TTGTGCA204.078291E-450.2221850
GTAGGAA550.050.22217649
AGGTATA150.00826620150.2221749
AACAAGC150.00826620150.2221750
GGAATAA150.00826620150.2221750
TCACAAG150.00826620150.2221750
TATTATT301.0084968E-650.2221749
CTGTTAC301.0084968E-650.2221749
ATCATCT150.00826620150.2221750
CTAATCG150.00826620150.2221749
GAAATGA204.0954296E-450.17954345
CACATCC402.564775E-950.17954345
GATCCTC150.008294011550.17954345
TATATTA301.0144267E-650.17954347
AAGGTAA150.008294011550.17954346
CATTCTA150.008294011550.17954345
TTATCTC252.0324267E-550.17954347
CATTCCC402.564775E-950.17954347
Produced by FastQC (version 0.11.5)