FastQCFastQC Report
Tue 31 May 2016
SRR212502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16015
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTC2491.5547923821417422No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAA2041.273805807055885No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC1951.2176084920387136No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1250.7805182641273806No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1210.7555416796753044No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC730.45582266625039025No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAAT550.34342803621604745No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGACGCTGAAAAACATCGAAC480.29971901342491414No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCC480.29971901342491414No Hit
CATCCCGGGAGTTTCAGCATGGCCCTTTCTTTATGAGCTGGATTTAATAGACTCAAAA440.27474242897283796No Hit
CATCCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC390.2435216984077427No Hit
CATCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTT340.21230096784264751No Hit
CATCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.19981267561660943No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC300.18732438339057134No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC260.16234779893849516No Hit
CATCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1561036528254761No Hit
CATCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA230.14361536059943802No Hit
CATCCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG200.1248829222603809No Hit
CATCCCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTT200.1248829222603809No Hit
CATCCCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA190.11863877614736185No Hit
CATCCCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC180.11239463003434282No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTC180.11239463003434282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCT950.052.13166451
TTCGAGG203.3040004E-452.13166444
CCCTGTA203.3040004E-452.13166451
AGGCCGC950.052.13166450
AGGCCCT203.3040004E-452.13166448
GCCGCTC950.052.13166452
CAGGCCG950.052.13166449
TTTCGAG203.3040004E-452.13166443
GCCCTGT203.3040004E-452.13166450
TAGCGCA1000.052.1316644
CGCAGGC1000.052.1316647
CCCGGGC950.051.9687544
CGGGATC950.051.9687546
CCGGGGG3800.051.9687545
GGCACTG1100.051.9687521
TGGCACT1100.051.9687520
GGGTTCG550.051.9687531
TTCGCTA1100.051.9687534
TAGTGAC203.3557735E-451.9687514
CGGGGTT251.588036E-551.968756
Produced by FastQC (version 0.11.5)