FastQCFastQC Report
Tue 31 May 2016
SRR212501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16405
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTC2651.6153611703748856No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAA2111.286193233770192No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC1911.164279183175861No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1160.7071014934471198No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1000.609570252971655No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC640.3901249619018592No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCC520.3169765315452606No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAAT500.3047851264858275No Hit
CATCCCGGGAGTTTCAGCATGGCCCTTTCTTTATGAGCTGGATTTAATAGACTCAAAA440.2682109113075282No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGACGCTGAAAAACATCGAAC410.24992380371837855No Hit
CATCCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC400.24382810118866197No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC370.22554099359951232No Hit
CATCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTT360.2194452910697958No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC340.20725388601036268No Hit
CATCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1950624809509296No Hit
CATCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1950624809509296No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT290.17677537336177995No Hit
CATCCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG250.15239256324291375No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTC230.14020115818348067No Hit
CATCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA210.12800975312404755No Hit
CATCCCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA180.1097226455348979No Hit
CATCCCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA180.1097226455348979No Hit
CATCCCGGGAGCGTGGACATCCTGTCAGCATGAATGAATTCTTCAATCCATGCTTTTA170.10362694300518134No Hit
CATCCCGGGACTGGTCGGATGAGGCACCTAAAGATTGTCTACCGCAGATTCAGACATG170.10362694300518134No Hit
CATCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA170.10362694300518134No Hit
CATCCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG170.10362694300518134No Hit
CATCCCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTT170.10362694300518134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCT353.527566E-852.09174351
TAAGATT150.007044885352.09174327
ACTTCCA150.007044885352.09174340
AGCGCAG503.6379788E-1252.09174345
TAGCGCA401.6789272E-952.0917444
ACCGTAG458.0035534E-1152.0917440
CGTAGCG458.0035534E-1152.0917442
TACCGTA458.0035534E-1152.0917439
CTACCGT458.0035534E-1152.0917438
CCCGGGG6250.051.932934
CGGGATG950.051.932936
CCGGGGT950.051.932935
CCGGGAC950.051.932935
ATCCCGG16400.051.9329262
GGATGGG150.00713085651.9329268
GGGTTCG401.724402E-951.93292631
TTCGCTA550.051.93292634
CTGGGTT401.724402E-951.93292629
CGGGGTC203.3697273E-451.9329266
CGGGGGA203.3697273E-451.9329266
Produced by FastQC (version 0.11.5)