FastQCFastQC Report
Tue 31 May 2016
SRR212500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16750
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTC2771.653731343283582No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAA1701.0149253731343284No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC1320.7880597014925373No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1030.6149253731343283No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT920.5492537313432836No Hit
CATCCCGGGAGTTTCAGCATGGCCCTTTCTTTATGAGCTGGATTTAATAGACTCAAAA560.33432835820895523No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAAT550.3283582089552239No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCC420.2507462686567164No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGACGCTGAAAAACATCGAAC380.22686567164179106No Hit
CATCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.191044776119403No Hit
CATCCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC320.191044776119403No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC310.18507462686567164No Hit
CATCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTT310.18507462686567164No Hit
CATCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1791044776119403No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC250.1492537313432836No Hit
CATCCCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA240.14328358208955225No Hit
CATCCCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA240.14328358208955225No Hit
CATCCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG200.11940298507462686No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT180.10746268656716418No Hit
CATCCCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA170.10149253731343284No Hit
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC170.10149253731343284No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTC170.10149253731343284No Hit
CATCCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.10149253731343284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGC203.2879526E-452.1957868
GGATGCA203.2879526E-452.1957868
CGGGGTG203.2879526E-452.1957866
CGGGGGA457.8216544E-1152.1957866
CGGGGCT401.6516424E-952.1957866
CCCGGGT457.8216544E-1152.1957864
CGGGGAT203.2879526E-452.1957866
CGGGGAA401.6516424E-952.1957866
GGATCTC203.2879526E-452.1957868
GGATCCC203.2879526E-452.1957868
GGGAGTG251.5501517E-552.1957867
GGGAGAT203.2879526E-452.1957867
CGGGATG900.052.1957866
CGGGATA251.5501517E-552.1957866
CGGGAGT503.6379788E-1252.1957866
CGGGAGG900.052.1957866
CGGGACT251.5501517E-552.1957866
CGGGAAC401.6516424E-952.1957866
CCGGGCT203.2879526E-452.1957865
CCGGGAT3600.052.1957865