SRR212499_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212499_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25523
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT	186	0.7287544567644869	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGC	164	0.6425576930611605	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGC	116	0.45449202679935746	No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	113	0.4427379226579948	No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	101	0.39572150609254403	No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	100	0.3918034713787565	No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	92	0.3604591936684559	No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	72	0.2820984993927046	No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	50	0.19590173568937824	No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	49	0.19198370097559064	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGC	42	0.1645574579790777	No Hit
CATCCCGGGAGTTTCAGCATGGCCCTTTCTTTATGAGCTGGATTTAATAGACTCAA	40	0.15672138855150258	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGC	39	0.15280335383771498	No Hit
CATCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTT	39	0.15280335383771498	No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGACGCTGAAAAACATCGA	36	0.1410492496963523	No Hit
CATCCCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC	35	0.13713121498256475	No Hit
CATCCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	32	0.12537711084120207	No Hit
CATCCCGGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTCT	30	0.11754104141362694	No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	28	0.1097049719860518	No Hit
CATCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.1097049719860518	No Hit
CATCCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	26	0.10186890255847666	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGGGT	45	1.2369128E-10	50.08268	28
GGTTCGC	45	1.2369128E-10	50.08268	32
GTTCGCT	35	4.955109E-8	50.08268	33
ACCGGAG	30	9.961841E-7	50.082676	40
GAGCGCA	15	0.008282739	50.082676	44
CGGAGCG	30	9.961841E-7	50.082676	42
ATCTCAT	15	0.008282739	50.082676	49
CACAATT	20	4.073156E-4	50.082676	42
TTATCTC	15	0.008282739	50.082676	47
TTATCAC	15	0.008282739	50.082676	38
ACCGTAG	20	4.073156E-4	50.082676	40
CAATTAT	15	0.008282739	50.082676	44
CGTAGCG	15	0.008282739	50.082676	42
CGCTACC	40	2.4720066E-9	50.082676	36
GCTACCG	40	2.4720066E-9	50.082676	37
CCGTAGC	20	4.073156E-4	50.082676	41
TACCGTA	15	0.008282739	50.082676	39
TACCGGA	25	2.0100953E-5	50.082676	39
TATCTCA	15	0.008282739	50.082676	48
TCGCTAC	40	2.4720066E-9	50.082676	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content