##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25523 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.51424205618462 39.0 38.0 40.0 35.0 40.0 2 36.967049328057044 39.0 36.0 40.0 31.0 40.0 3 38.063550523057636 39.0 38.0 40.0 35.0 40.0 4 38.23731536261411 39.0 38.0 40.0 35.0 40.0 5 38.40105003330329 39.0 38.0 40.0 35.0 40.0 6 38.52501665164753 39.0 38.0 40.0 36.0 40.0 7 38.538377150021546 39.0 38.0 40.0 36.0 40.0 8 38.577322415076594 39.0 38.0 40.0 36.0 40.0 9 38.55279551776829 39.0 38.0 40.0 36.0 40.0 10 37.176076480037615 39.0 36.0 40.0 33.0 40.0 11 37.623594405046425 39.0 38.0 40.0 33.0 40.0 12 37.35501312541629 39.0 37.0 40.0 33.0 40.0 13 37.36739411511186 39.0 37.0 40.0 33.0 40.0 14 37.141793676291975 39.0 36.0 40.0 33.0 40.0 15 37.368608705873136 39.0 37.0 40.0 33.0 40.0 16 37.310700152803356 39.0 37.0 40.0 33.0 40.0 17 37.07710692316734 39.0 36.0 40.0 33.0 40.0 18 37.01238098969557 39.0 36.0 40.0 32.0 40.0 19 37.020256239470285 39.0 36.0 40.0 32.0 40.0 20 36.914038318379504 39.0 36.0 40.0 32.0 40.0 21 36.84664812130235 39.0 36.0 40.0 32.0 40.0 22 36.71817576303726 38.0 36.0 40.0 31.0 40.0 23 36.46644203267641 38.0 35.0 40.0 31.0 40.0 24 36.401010852956155 38.0 35.0 40.0 31.0 40.0 25 36.3965051130353 38.0 35.0 40.0 31.0 40.0 26 35.92007209183873 38.0 35.0 40.0 30.0 40.0 27 35.89037338870823 38.0 35.0 40.0 30.0 40.0 28 35.578380284449324 38.0 35.0 39.0 29.0 40.0 29 35.525800258590294 38.0 35.0 39.0 29.0 40.0 30 35.21490420405125 38.0 35.0 39.0 29.0 40.0 31 34.82122007600987 38.0 34.0 39.0 28.0 40.0 32 34.64976687693453 38.0 34.0 39.0 27.0 40.0 33 33.988480977941464 38.0 33.0 39.0 23.0 40.0 34 34.633663754260866 38.0 34.0 39.0 27.0 40.0 35 34.378717235434706 38.0 33.0 39.0 27.0 40.0 36 33.51839517298123 38.0 33.0 39.0 22.0 40.0 37 33.23845159268111 38.0 33.0 39.0 20.0 40.0 38 32.96951768992673 37.0 33.0 39.0 18.0 40.0 39 32.88394781177761 37.0 33.0 39.0 16.0 40.0 40 32.581788974650316 37.0 32.0 39.0 15.0 40.0 41 32.11609136856953 36.0 32.0 39.0 2.0 40.0 42 31.973161462210555 36.0 32.0 39.0 2.0 40.0 43 31.680327547702074 36.0 31.0 39.0 2.0 40.0 44 31.52082435450378 36.0 31.0 39.0 2.0 40.0 45 31.296634408180857 36.0 31.0 39.0 2.0 40.0 46 30.692473455314815 36.0 30.0 39.0 2.0 40.0 47 30.454492026799358 35.0 30.0 39.0 2.0 40.0 48 30.281471613838498 35.0 30.0 38.0 2.0 40.0 49 30.015554597813736 35.0 29.0 38.0 2.0 40.0 50 29.73055675273283 35.0 29.0 38.0 2.0 40.0 51 29.190612388825766 35.0 29.0 38.0 2.0 40.0 52 28.98797163342867 35.0 29.0 38.0 2.0 40.0 53 28.67186459272029 35.0 28.0 38.0 2.0 39.0 54 28.26098029228539 34.0 27.0 38.0 2.0 39.0 55 27.847941072757905 34.0 26.0 38.0 2.0 39.0 56 15.684833287622928 20.0 2.0 23.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.2108268982346857 1 2 -0.23253911602640187 1 3 -0.6775952725714731 1 4 -1.1522230844658452 1 5 -0.9836382177541907 1 6 -0.46858596835472355 1 7 -0.7394009405371449 1 8 -0.7492776471220495 1 9 -0.7505882001609905 1 10 -0.2775350758194577 1 11 -0.33904689080368655 1 12 0.8530357370617452 1 13 0.2720061676398302 1 14 -0.13231047206704716 1 15 0.8560460833801002 1 16 0.1931970879122531 1 17 -0.49179691061615927 1 18 0.18380939449635747 1 19 -0.30161500819046694 1 20 0.05241952278782236 1 21 -0.637611198388278 1 22 -1.111408867748601 1 23 -0.770148429959896 1 24 -0.8354776702358322 1 25 0.0739740597974432 1 26 -1.7636627258342443 1 27 -1.0636757242179655 1 28 0.0918674787764786 1 29 -0.6256494139480893 1 30 -0.38857199694565026 1 31 -0.7816881519750609 1 32 0.03182414124286481 1 33 -1.092532558523537 1 34 -0.2020653251594382 1 35 0.3002796705967228 1 36 -0.5902684496933759 1 37 -1.7093794930992203 1 38 -1.4319419950165013 1 39 0.19877219688891756 1 40 0.7636457794872769 1 41 1.4025587274575102 1 42 1.9909710065278077 1 43 -1.2746789189950043 1 44 -1.0484359573770483 1 45 -1.8832332555002367 1 46 -4.48016616812567 1 47 -4.802913048716693 1 48 -4.900481034888237 1 49 -5.232990161010331 1 50 -5.546783272766319 1 51 -7.858675984642765 1 52 -5.486181169057691 1 53 -4.972070770650511 1 54 -5.444913718925076 1 55 -6.035894890318261 1 56 -0.4908093716499291 2 1 -0.06161892219874687 2 2 0.03061877871044061 2 3 -0.10483985461482348 2 4 -0.963368595301759 2 5 -0.9341026140390198 2 6 -0.3447469590667964 2 7 -0.8229922718064984 2 8 -1.1269866254502219 2 9 -2.416222875083591 2 10 -1.2249034968720878 2 11 -0.6610283149522971 2 12 -0.41631410813949543 2 13 -0.3595727797285875 2 14 -0.8320008745438301 2 15 -0.9365235760626263 2 16 -0.2030877418091066 2 17 -0.29365449575547586 2 18 -0.25581908847578205 2 19 -2.2087357512245234 2 20 -1.3005216536827646 2 21 -1.4890043872427654 2 22 -0.9720899822996856 2 23 -0.6772691729939524 2 24 -0.6961587847869168 2 25 -0.6164284169827425 2 26 -0.7512788249054552 2 27 -1.0234280461993919 2 28 -3.415872459304012 2 29 -0.7959280517189882 2 30 -2.7477051238806354 2 31 -0.6454652417583375 2 32 -0.9929436606147206 2 33 -1.909870019823849 2 34 -0.19896934992723914 2 35 -0.25389989596674667 2 36 1.991774893959878 2 37 0.5290105997800367 2 38 0.9240951566862847 2 39 0.4402582650003737 2 40 1.0484755008495057 2 41 0.8638590370550361 2 42 2.1674415947631047 2 43 1.00395885190283 2 44 0.30140924386134316 2 45 0.5254354751499193 2 46 1.9625582900786611 2 47 0.9896566107260334 2 48 -0.42679682436192223 2 49 2.277845752302362 2 50 2.360337470267737 2 51 2.8719741701559975 2 52 2.6345618649980374 2 53 3.520189291268995 2 54 3.877067705223535 2 55 3.363485914635298 2 56 1.273896510703013 3 1 1.151616371918898 3 2 0.7806187787104406 3 3 1.3289836747969446 3 4 1.367513757639415 3 5 1.1578091506668642 3 6 1.1037824526979065 3 7 1.0961253752523277 3 8 1.086248668667423 3 9 1.0323065366811122 3 10 1.8375965031279122 3 11 1.210295214459471 3 12 1.9550094212722726 3 13 1.7654272202714125 3 14 1.815057948985583 3 15 1.4053881886432578 3 16 1.4366181405438354 3 17 2.261492563068053 3 18 2.108151499759515 3 19 1.7839113075990056 3 20 1.6774195227878224 3 21 1.7867309068748796 3 22 1.8293806059356115 3 23 1.5028778858295766 3 24 1.8553118034483802 3 25 0.8798951124290255 3 26 1.0685741162710158 3 27 2.344219012624137 3 28 2.032656952460691 3 29 3.009219007104541 3 30 2.4140595820017197 3 31 2.8104171111828364 3 32 1.9298504570323374 3 33 3.042335862529093 3 34 2.584118885366877 3 35 2.9483059863861953 3 36 2.274863129253994 3 37 2.1393047174270947 3 38 2.9167422155098137 3 39 2.5836406179415476 3 40 1.6550931479083317 3 41 1.294006095878565 3 42 1.9284710065278077 3 43 2.606900028373417 3 44 2.4153798320966366 3 45 2.0805825339734483 3 46 3.631675937137487 3 47 2.3977448460201494 3 48 4.135703175638078 3 49 2.174904575831775 3 50 3.180190411444208 3 51 2.180797699567762 3 52 2.3000030414686243 3 53 2.656218703033698 3 54 2.630744175811767 3 55 1.8193682675764755 3 56 -0.1783093716499291 4 1 -0.64729667155936 4 2 -1.3606855691156454 4 3 -0.6981902382465321 4 4 0.0033833228568056484 4 5 -0.44816911020269856 4 6 -0.43426102556296087 4 7 -0.5288746247476723 4 8 -1.0604904617673583 4 9 -0.9405195502754111 4 10 -2.0509904533938297 4 11 -1.3413352203231383 4 12 -2.3140123178581646 4 13 -2.098703214511197 4 14 -3.402333355362245 4 15 -2.059285724400219 4 16 -1.3949035985866018 4 17 -1.5238335238884702 4 18 -1.5141311089361338 4 19 -0.7432626054444711 4 20 -1.078015259820873 4 21 -0.6398995279077297 4 22 -2.0130106984122165 4 23 -0.6900568967791187 4 24 -0.8675142835081431 4 25 -0.8619527136579279 4 26 -1.6515345793811562 4 27 -1.7318679438976048 4 28 -0.8532126127566997 4 29 -2.2000201233302406 4 30 -1.205505635389585 4 31 -1.7221915844693356 4 32 -1.7576495429676626 4 33 -1.4929902244274302 4 34 -0.8153376363722558 4 35 -1.1848461875268512 4 36 -0.4072020881373106 4 37 1.4599568913401413 4 38 1.4330465633358997 4 39 1.102662357071985 4 40 2.255636626169199 4 41 -0.6706678171649116 4 42 0.03444926739737042 4 43 -1.2083173629309307 4 44 0.5213580929661994 4 45 0.25177818614736225 4 46 2.908849850180964 4 47 0.938505715585368 4 48 1.921029262594601 4 49 2.750991532353517 4 50 2.633994759270294 4 51 4.756884656089504 4 52 2.944024780599058 4 53 2.1698056595554363 4 54 2.557374610594376 4 55 3.025890006706909 4 56 3.509190628350071 5 1 -0.7461109008083753 5 2 -1.1057448576531925 5 3 -0.5914708706576022 5 4 0.015241030366688335 5 5 -0.4046908493331358 5 6 -0.06667209275664021 5 7 0.1074890116159608 5 8 0.14306685048560297 5 9 0.08912471849929204 5 10 0.0023692304006388554 5 11 -0.9658411491768959 5 12 -0.6302178514550008 5 13 -0.45048187063768097 5 14 -0.9576693237416904 5 15 -0.8162027204476487 5 16 -0.48383640491071134 5 17 -1.0850983460228534 5 18 -0.7157121366041181 5 19 -2.5910886924009944 5 20 -1.5839441135758108 5 21 -1.4462236385796672 5 22 -0.8410739395189353 5 23 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-0.67069302122853 7 33 1.5939662973117024 7 34 0.5324884505842675 7 35 0.6412407689948907 7 36 2.5927979118626894 7 37 2.503435152209704 7 38 0.25913351985764166 7 39 -2.2451637298845384 7 40 -2.222624243396016 7 41 -1.2793634693388256 7 42 -0.7916376891243679 7 43 -1.2083173629309307 7 44 -1.696033211381625 7 45 -0.7482218138526378 7 46 -2.2215849324277315 7 47 -1.974537762675503 7 48 -2.731144650448879 7 49 -1.8577041198204007 7 50 -2.192092197251444 7 51 -1.069202300432238 7 52 -0.6211926107052896 7 53 -0.04758564479238814 7 54 -0.26871234592736215 7 55 -2.191501297640915 7 56 0.2048428022631139 8 1 -0.993383628081105 8 2 -1.4493812212895563 8 3 0.07398367479694201 8 4 -0.6174862423605845 8 5 -0.8846908493331327 8 6 -0.3212175473020906 8 7 -0.7688746247476743 8 8 -0.05875133133258004 8 9 -0.1526934633188901 8 10 -0.30490349687208607 8 11 -0.4022047855405262 8 12 -1.8774905787277305 8 13 -0.9595727797285889 8 14 0.3750579489855852 8 15 -0.4471118113567414 8 16 0.04161814054383228 8 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116 29 1.139900825286361 116 30 0.027695945638086528 116 31 1.0604171111828364 116 32 1.3787140933959705 116 33 1.7639267716199996 116 34 1.8170734308214236 116 35 2.101715077295289 116 36 2.3714540383449005 116 37 2.855213808336188 116 38 3.2690149427825403 116 39 3.918867890668821 116 40 3.2516840569992382 116 41 4.299687914060385 116 42 3.900061915618714 116 43 3.2659909374643235 116 44 3.8415161957330035 116 45 5.552173443064355 116 46 3.2112213916829404 116 47 3.505699391474696 116 48 3.6186577210926245 116 49 1.6976318485590483 116 50 1.0608722296260247 116 51 1.4762522450223088 116 52 1.4420484960140776 116 53 2.298264157579151 116 54 1.0455169030844935 116 55 1.342095540303749 116 56 -0.8999002807408374 117 1 -0.378145532843007 117 2 -0.4931907450990849 117 3 -1.1412544204411503 117 4 -0.49558148045582584 117 5 0.2619758173335285 117 6 -0.045027071111618966 117 7 0.37588728001423277 117 8 0.4136296210483721 117 9 0.16921129858587136 117 10 0.29890602693743773 117 11 0.44541426207852197 117 12 -0.21463343587058148 117 13 0.3547129345571278 117 14 0.4341055680332033 117 15 0.5928881886432578 117 16 0.3235229024485946 117 17 -0.7474360083605163 117 18 -0.126967547859536 117 19 0.09938749807519542 117 20 -0.13805666768836744 117 21 -0.42457861693464594 117 22 -0.9384765369215344 117 23 -0.4643840189323285 117 24 0.5279308510674312 117 25 -0.4623667923328796 117 26 0.6548836400805413 117 27 0.6180285364336626 117 28 0.1219426667464063 117 29 1.0836237690093 117 30 1.0926310105731503 117 31 -3.1300590792933534 117 32 -2.6624114477295677 117 33 -1.192783185089958 117 34 -2.2998096860616926 117 35 -2.251098775518564 117 36 -2.5852559183650534 117 37 -1.0452190920967155 117 38 -0.005876832109237284 117 39 -0.18719271539178806 117 40 -0.08597828066309887 117 41 -0.8280177136452451 117 42 -1.5328385172817178 117 43 -1.1192904478170576 117 44 -0.9506915964747904 117 45 -2.071203180312267 117 46 -0.9338002533387026 117 47 -1.0159456301703251 117 48 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13 -0.7881442083000181 119 14 0.14839128231891863 119 15 -0.5023499065948371 119 16 -0.10504852612282889 119 17 0.6335163725918633 119 18 0.5396991188071354 119 19 0.05176845045615153 119 20 -1.2809138105455133 119 21 0.14684995449393057 119 22 1.2043806059356115 119 23 0.011806457258145997 119 24 1.1469784701150445 119 25 0.8709665410004561 119 26 1.0834550686519648 119 27 1.284695203100327 119 28 0.7886093334130706 119 29 0.7502904356759714 119 30 0.8545357724779095 119 31 2.536607587373311 119 32 1.7661599808418629 119 33 2.3310263387195675 119 34 2.4144760282240227 119 35 1.4631869387671443 119 36 1.2242678911587532 119 37 1.9071618602842406 119 38 0.9941231678907627 119 39 0.765188236989168 119 40 2.104497909813091 119 41 0.5053156196880906 119 42 1.9909710065278077 119 43 2.118804790278176 119 44 0.8112131654299723 119 45 0.3573682482591636 119 46 0.5423902228517719 119 47 0.841197226972529 119 48 1.9541555565904574 119 49 0.9725236234508223 119 50 1.9569761257299234 119 51 3.121273890043952 119 52 3.368455422421004 119 53 4.581813941128939 119 54 5.294434652002241 119 55 5.831273029481235 119 56 3.223476342635786 120 1 -2.7400502947477676 120 2 -3.502714554622891 120 3 -3.0460163252030554 120 4 -2.390819575693918 120 5 -2.0713575159998 120 6 -2.2545508806354277 120 7 -2.728874624747675 120 8 -2.605417997999247 120 9 -2.459360129985555 120 10 -1.6915701635387563 120 11 -2.0022047855405276 120 12 -1.9574905787277288 120 13 -2.2929061130619175 120 14 -1.8516087176810814 120 15 -1.8737784780234108 120 16 -3.3050485261228317 120 17 -3.242674103598617 120 18 -2.3460151669071507 120 19 -2.624422025734326 120 20 -3.414247143878846 120 21 -2.234102426458456 120 22 -2.262286060731057 120 23 -1.2929554475037577 120 24 -2.186354863218284 120 25 -3.157604887570976 120 26 -1.8689258837289842 120 27 -1.3724476540425314 120 28 -1.3256763808726433 120 29 -0.9449476595621249 120 30 -0.6026070846649461 120 31 0.060417111182836436 120 32 -0.35764954296766405 120 33 -2.078497470804237 120 34 0.27161888536687684 120 35 -0.022527346947136095 120 36 -1.0709702040793374 120 37 -1.2356952825729053 120 38 -3.2915911178235184 120 39 -0.7205260487251195 120 40 -3.457406852091669 120 41 -1.7518272374547692 120 42 -2.142362326805525 120 43 -2.309766638293251 120 44 -1.0554535012366983 120 45 -0.8235841326932167 120 46 -1.2766573961958478 120 47 -0.5397551539798506 120 48 -0.49346349102858866 120 49 -0.6084287575015601 120 50 0.4426904114442074 120 51 -0.602535633765573 120 52 1.387503041468623 120 53 0.21038536970036503 120 54 1.284910842478432 120 55 0.2693682675764748 120 56 1.7758572950167366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 2.0 4 2.0 5 3.0 6 16.0 7 14.0 8 17.0 9 24.0 10 29.0 11 38.0 12 44.0 13 59.0 14 66.0 15 98.0 16 93.0 17 122.0 18 138.0 19 138.0 20 175.0 21 206.0 22 225.0 23 304.0 24 571.0 25 468.0 26 399.0 27 395.0 28 411.0 29 502.0 30 556.0 31 681.0 32 836.0 33 1082.0 34 1406.0 35 1982.0 36 2961.0 37 4304.0 38 4710.0 39 2433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 27.71225953061944 67.49206597970459 1.6847549269286528 3.110919562747326 11 27.68483328762293 13.536809936136034 47.91364651490812 10.864710261332917 12 46.88320338518199 13.078399874622889 22.254437174313367 17.783959565881755 13 30.705638051953137 20.581436351526076 28.221604043411823 20.49132155310896 14 21.991928848489597 27.79453825960898 29.777063824785486 20.436469067115933 15 23.637503428280375 31.36386788386945 27.70442346119187 17.294205226658306 16 28.444932022097714 26.595619637189987 28.029620342436235 16.929827998276064 17 26.427144144497124 24.280061121341532 27.688751322336714 21.604043411824627 18 26.27825882537319 24.765897425851193 25.251733730360854 23.704110018414763 19 24.413274301610315 26.047094777259726 26.815029581162086 22.724601339967872 20 26.544685185910748 25.09893037652314 28.362653293108174 19.99373114445794 21 31.007326724914787 23.151667123770718 26.552521255338323 19.28848489597618 22 28.488030403949377 24.88735650197861 25.83160286800141 20.793010226070603 23 22.70892920111272 26.20381616581123 27.598636523919602 23.488618109156448 24 23.284880304039493 29.420522665830816 25.46722563961917 21.82737139051052 25 21.673003802281368 30.841597742150444 24.115087609266592 23.370310846301596 26 22.153387703889585 30.32465495608532 26.125313676286073 21.39664366373902 27 25.115731659474307 24.252648097293054 27.183209101608476 23.448411141624167 28 24.764595103578156 26.8793157564344 27.067639673571875 21.288449466415567 29 23.052470468191984 25.297280326517797 26.72579569090695 24.924453514383266 30 22.010602788140584 25.0147260946397 31.034753583349694 21.93991753387002 31 25.70497211530909 26.447254732542614 27.825779593119158 20.02199355902914 32 27.642020900447868 24.758387679735993 28.137031507818023 19.462559911998113 33 28.436652384134597 23.53079916663391 28.027831282676207 20.004717166555288 34 22.91904218928164 25.27424999017025 32.27303110132505 19.533676719223056 35 21.722713864306783 25.99410029498525 31.92527040314651 20.357915437561456 36 21.58290369554095 28.088472588452913 28.356094297296234 21.972529418709904 37 26.35805384978743 26.27538970240907 26.9248937175248 20.441662730278694 38 22.838582677165352 26.830708661417326 27.27952755905512 23.051181102362207 39 23.004842710342928 25.363203275719513 28.827906610496477 22.80404740344108 40 23.091467738123374 28.496021429134167 27.133065469156225 21.279445363586227 41 22.992082561941153 25.158545712372476 28.471264820577463 23.378106905108915 42 22.441736661540283 24.795930438897432 28.10047714815253 24.661855751409757 43 24.417458502543074 24.969443677798367 29.345897567322478 21.267200252336078 44 22.62134773865384 24.84523480935294 29.916012775521473 22.617404676471747 45 24.015623767063836 26.765564586127987 27.80320366132723 21.415607985480946 46 25.08089337858101 27.266987609502014 26.667192802462313 20.98492620945466 47 22.066079816839697 27.339833418860778 26.949038803142145 23.645047961157385 48 24.327526958170402 25.129359718766047 28.53813643006675 22.0049768929968 49 23.117006158218853 26.219801042160114 27.767250907942525 22.895941891678508 50 22.034433738745854 27.021797504343702 29.25683146422366 21.68693729268678 51 24.036334913112164 24.37993680884676 28.649289099526065 22.93443917851501 52 24.245417193426043 26.576327433628315 27.259797724399494 21.918457648546145 53 20.61089817046667 25.581854822776307 29.38317461571897 24.424072391038052 54 20.483023044389107 26.3844420728092 29.775880469583775 23.356654413217914 55 24.742145820983996 26.291246789172103 28.314562339458604 20.652045050385297 56 22.376378512984704 25.657140598442624 27.732321435629864 24.234159452942805 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 13.0 13 52.5 14 79.0 15 97.0 16 115.0 17 111.0 18 107.0 19 101.5 20 96.0 21 79.5 22 71.0 23 79.0 24 108.0 25 137.0 26 176.0 27 215.0 28 274.0 29 413.0 30 493.0 31 515.5 32 538.0 33 571.0 34 604.0 35 614.5 36 625.0 37 713.5 38 840.0 39 878.0 40 915.5 41 953.0 42 918.0 43 883.0 44 927.5 45 972.0 46 1052.0 47 1254.5 48 1377.0 49 1429.0 50 1481.0 51 1341.5 52 1202.0 53 1279.0 54 1469.5 55 1583.0 56 1599.5 57 1616.0 58 1426.0 59 1236.0 60 1243.0 61 1250.0 62 1292.0 63 1244.5 64 1155.0 65 999.0 66 843.0 67 676.0 68 509.0 69 515.5 70 522.0 71 480.0 72 345.5 73 253.0 74 181.0 75 109.0 76 82.0 77 55.0 78 41.0 79 24.5 80 22.0 81 15.0 82 8.0 83 7.0 84 6.0 85 7.5 86 9.0 87 5.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.04701641656545077 26 0.07444265956196372 27 0.1292951455549896 28 0.13713121498256475 29 0.1645574579790777 30 0.22724601339967873 31 0.23900011754104142 32 0.2703443952513419 33 0.3291149159581554 34 0.35262312424088077 35 0.3839674019511813 36 0.4466559573717823 37 0.4662461309407201 38 0.4819182697958704 39 0.4858363045096579 40 0.5367707557888962 41 0.5328527210751087 42 0.6425576930611605 43 0.6268855542060103 44 0.6347216236335854 45 0.6934921443403989 46 0.7091642831955491 47 0.7444265956196372 48 0.8071151510402382 49 0.7483446303334248 50 0.7796889080437253 51 0.7953610468988755 52 0.826705324609176 53 0.8462954981781139 54 0.8776397758884144 55 0.854131567605689 56 0.8776397758884144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 25523.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.44834071229871 #Duplication Level Percentage of deduplicated Percentage of total 1 68.99097080340077 41.013987383928225 2 17.445462334409807 20.742075774791367 3 7.249719897185791 12.929514555498962 4 3.2623739537336056 7.757708733299378 5 1.364265471561326 4.055165928770129 6 0.553614973966915 1.9746894957489325 7 0.2899887958874316 1.2067546918465697 8 0.19112897910762539 0.9089840535987149 9 0.12522243458775456 0.6699839360576735 >10 0.4745271205430699 5.0425106766445955 >50 0.019771963355961247 1.0343611644399169 >100 0.03295327225993541 2.6642636053755435 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 186 0.7287544567644869 No Hit CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGC 164 0.6425576930611605 No Hit CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGC 116 0.45449202679935746 No Hit CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 113 0.4427379226579948 No Hit CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 101 0.39572150609254403 No Hit CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 100 0.3918034713787565 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 92 0.3604591936684559 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 72 0.2820984993927046 No Hit CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 50 0.19590173568937824 No Hit CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA 49 0.19198370097559064 No Hit CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGC 42 0.1645574579790777 No Hit CATCCCGGGAGTTTCAGCATGGCCCTTTCTTTATGAGCTGGATTTAATAGACTCAA 40 0.15672138855150258 No Hit CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGC 39 0.15280335383771498 No Hit CATCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTT 39 0.15280335383771498 No Hit CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGACGCTGAAAAACATCGA 36 0.1410492496963523 No Hit CATCCCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC 35 0.13713121498256475 No Hit CATCCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 32 0.12537711084120207 No Hit CATCCCGGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTCT 30 0.11754104141362694 No Hit CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 28 0.1097049719860518 No Hit CATCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.1097049719860518 No Hit CATCCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 26 0.10186890255847666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.003918034713787565 0.0 0.0 0.0 0.0 15 0.003918034713787565 0.0 0.0 0.0 0.0 16 0.003918034713787565 0.0 0.0 0.0 0.0 17 0.003918034713787565 0.0 0.0 0.0 0.0 18 0.003918034713787565 0.0 0.0 0.0 0.0 19 0.00783606942757513 0.0 0.0 0.0 0.0 20 0.00783606942757513 0.0 0.0 0.0 0.0 21 0.00783606942757513 0.0 0.0 0.0 0.0 22 0.00783606942757513 0.0 0.0 0.0 0.0 23 0.00783606942757513 0.0 0.0 0.0 0.0 24 0.00783606942757513 0.0 0.0 0.0 0.0 25 0.00783606942757513 0.0 0.0 0.0 0.0 26 0.00783606942757513 0.0 0.0 0.0 0.0 27 0.00783606942757513 0.0 0.0 0.0 0.0 28 0.00783606942757513 0.0 0.0 0.0 0.0 29 0.011754104141362692 0.0 0.0 0.0 0.0 30 0.01567213885515026 0.0 0.0 0.0 0.0 31 0.023508208282725384 0.0 0.0 0.0 0.0 32 0.023508208282725384 0.0 0.0 0.0 0.0 33 0.03134427771030052 0.0 0.0 0.0 0.0 34 0.03134427771030052 0.0 0.0 0.0 0.0 35 0.03134427771030052 0.0 0.0 0.0 0.0 36 0.03134427771030052 0.0 0.0 0.0 0.0 37 0.03134427771030052 0.0 0.0 0.0 0.0 38 0.03134427771030052 0.0 0.0 0.0 0.0 39 0.03134427771030052 0.0 0.0 0.0 0.0 40 0.03918034713787564 0.0 0.0 0.0 0.0 41 0.04701641656545077 0.0 0.0 0.0 0.0 42 0.05093445127923833 0.0 0.0 0.0 0.0 43 0.05877052070681346 0.0 0.0 0.0 0.0 44 0.06268855542060103 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGGGT 45 1.2369128E-10 50.08268 28 GGTTCGC 45 1.2369128E-10 50.08268 32 GTTCGCT 35 4.955109E-8 50.08268 33 ACCGGAG 30 9.961841E-7 50.082676 40 GAGCGCA 15 0.008282739 50.082676 44 CGGAGCG 30 9.961841E-7 50.082676 42 ATCTCAT 15 0.008282739 50.082676 49 CACAATT 20 4.073156E-4 50.082676 42 TTATCTC 15 0.008282739 50.082676 47 TTATCAC 15 0.008282739 50.082676 38 ACCGTAG 20 4.073156E-4 50.082676 40 CAATTAT 15 0.008282739 50.082676 44 CGTAGCG 15 0.008282739 50.082676 42 CGCTACC 40 2.4720066E-9 50.082676 36 GCTACCG 40 2.4720066E-9 50.082676 37 CCGTAGC 20 4.073156E-4 50.082676 41 TACCGTA 15 0.008282739 50.082676 39 TACCGGA 25 2.0100953E-5 50.082676 39 TATCTCA 15 0.008282739 50.082676 48 TCGCTAC 40 2.4720066E-9 50.082676 35 >>END_MODULE