SRR212498_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212498_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24403
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT	196	0.8031799368930049	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGC	172	0.7048313731918208	No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	129	0.5286235298938655	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGC	108	0.44256853665532925	No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	93	0.3811006843420891	No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	71	0.2909478342826702	No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	63	0.25816497971560876	No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	57	0.2335778387903127	No Hit
CATCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTT	50	0.2048928410441339	No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	50	0.2048928410441339	No Hit
CATCCCGGGAGTTTCAGCATGGCCCTTTCTTTATGAGCTGGATTTAATAGACTCAA	48	0.19669712740236858	No Hit
CATCCCGGGATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTAGTCT	47	0.19259927058148588	No Hit
CATCCCGGGATGACTGAGTGGGAAGCAGCCACACCAGCGGTGGCAGAGACCCCTGA	43	0.1762078432979552	No Hit
CATCCCGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA	42	0.17210998647707249	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGC	41	0.16801212965618983	No Hit
CATCCCGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGC	38	0.15571855919354177	No Hit
CATCCCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC	36	0.14752284555177642	No Hit
CATCCCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	36	0.14752284555177642	No Hit
CATCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.12703356144736302	No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGACGCTGAAAAACATCGA	31	0.12703356144736302	No Hit
CATCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29	0.11883784780559767	No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	26	0.10654427734294965	No Hit
CATCCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	25	0.10244642052206696	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCAT	15	0.008138537	50.29814	49
GCCGGCC	15	0.008138537	50.29814	48
CGCCGGC	15	0.008138537	50.29814	47
ACCGTAG	35	4.77703E-8	50.298134	40
CGTAGCG	35	4.77703E-8	50.298134	42
CCGTAGC	35	4.77703E-8	50.298134	41
TACCGTA	35	4.77703E-8	50.298134	39
CGCAGGC	35	4.77703E-8	50.298134	47
GCGCAGG	35	4.77703E-8	50.298134	46
CGCTACC	50	5.456968E-12	50.194218	36
GCTACCG	50	5.456968E-12	50.194218	37
TCGCTAC	50	5.456968E-12	50.194218	35
CTACCGT	35	4.856156E-8	50.19421	38
TTCGCTA	50	5.456968E-12	50.09072	34
GGTTCGC	45	1.2369128E-10	50.09072	32
ATAACAA	20	4.0662338E-4	50.09072	26
TAACAAT	20	4.0662338E-4	50.09072	27
GTTCGCT	45	1.2369128E-10	50.09072	33
AATAACA	20	4.1079396E-4	49.987656	25
ACTGGCT	50	7.2759576E-12	49.88501	24

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content