FastQCFastQC Report
Tue 31 May 2016
SRR212497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78809
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA10831.3742085294826734No Hit
GCTCAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10131.285386186856831No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8071.0239947214150669No Hit
GCTCAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5980.7587965841464807No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5390.6839320382189851No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5130.6509408823865295No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4440.5633874303696279No Hit
GCTCAAGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4300.5456229618444595No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACTGTAGCGCAGGCCGCTC3380.4288850258219239No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3170.4022383230341712No Hit
GCTCAAGGGGTGCAGTGCTGCTCAAGGGCACAACCTATCTCAGAAGTTGTAGATGAAG3120.3958938699894682No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2840.3603649329391313No Hit
GCTCAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2830.3590960423301907No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2790.3540204798944283No Hit
GCTCAAGGGGCCCTGGCGGCCCGGAGCCGCGCTCGTTGGGCTCCCATCCTGGGTCCCG2640.3349871207603192No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG2610.3311804489334974No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2500.31722265223515084No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2490.3159537616262102No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2470.313415980408329No Hit
GCTCAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2370.300727074318923No Hit
GCTCAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2250.2855003870116357No Hit
GCTCAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2020.2563159030060018No Hit
GCTCAAGGGGGCTAGTCCTTCTTAAAGCTCTAGCCAGTGGGAAAGCGTTTGTGTTTCT1940.246164778134477No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1870.23728254387189282No Hit
GCTCAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1840.233475872045071No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGA1780.22586252839142737No Hit
GCTCAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA1690.21444251291096195No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.21190473169308074No Hit
GCTCAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1650.20936695047519951No Hit
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1620.2055602786483777No Hit
GCTCAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC1600.2030224974304965No Hit
GCTCAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1580.2004847162126153No Hit
GCTCAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1480.18779581012320926No Hit
GCTCAAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT1480.18779581012320926No Hit
GCTCAAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT1440.18272024768744688No Hit
GCTCAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1440.18272024768744688No Hit
GCTCAAGGGGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTGCT1440.18272024768744688No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAA1440.18272024768744688No Hit
GCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1390.17637579464274386No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1360.17256912281592204No Hit
GCTCAAGGGGAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGCG1320.1674935603801596No Hit
GCTCAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1310.16622466977121902No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1290.16368688855333782No Hit
GCTCAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1290.16368688855333782No Hit
GCTCAAGGGATGTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGAAG1270.16114910733545662No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC1260.159880216726516No Hit
GCTCAAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA1250.15861132611757542No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACTGTAGCGCAGGCCGCTC1230.1560735448996942No Hit
GCTCAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1230.1560735448996942No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.1548046542907536No Hit
GCTCAAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1200.1522668730728724No Hit
GCTCAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1180.14972909185499117No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG1150.14592242002816935No Hit
GCTCAAGGGAGTGAAGTGGGGAAAATTAGCACCCTAAATCTTGTGATCTACACAGGTG1140.14465352941922877No Hit
GCTCAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG1100.13957796698346636No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG1100.13957796698346636No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1090.13830907637452575No Hit
GCTCAAGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT1060.13450240454770396No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1060.13450240454770396No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1040.13196462332982273No Hit
GCTCAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1000.12688906089406032No Hit
GCTCAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG990.1256201702851197No Hit
GCTCAAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCACGC980.12435127967617911No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA970.1230823890672385No Hit
GCTCAAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAAAG950.12054460784935729No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG950.12054460784935729No Hit
GCTCAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC930.1180068266314761No Hit
GCTCAAGGGGCCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATC910.1154690454135949No Hit
GCTCAAGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCTGGTATT900.1142001548046543No Hit
GCTCAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT900.1142001548046543No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA870.11039348297783248No Hit
GCTCAAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG870.11039348297783248No Hit
GCTCAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC860.10912459236889188No Hit
GCTCAAGGGGCCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA860.10912459236889188No Hit
GCTCAAGGGGCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCACG860.10912459236889188No Hit
GCTCAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC850.10785570175995127No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG850.10785570175995127No Hit
GCTCAAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGGGC840.10658681115101067No Hit
GCTCAAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCCTCG840.10658681115101067No Hit
GCTCAAGGGCTTCTTCTAGGCTCCGAGCGGTTGGTTTCTTGGGCCCTGTCTGTAGGCC830.10531792054207006No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCAA820.10404902993312946No Hit
GCTCAAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG820.10404902993312946No Hit
GCTCAAGGGAGACCAGAAATATTTTATTGTTCTTGGAATCATATTTTTGTGCTTCCAT820.10404902993312946No Hit
GCTCAAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT800.10151124871524825No Hit
GCTCAAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA800.10151124871524825No Hit
GCTCAAGGGGGCCATAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAA790.10024235810630765No Hit
GCTCAAGGGGACTTCGCCCTCCAGCCGCGGTGGCTGCAGCGCAACTTTCAGATAGCGG790.10024235810630765No Hit
GCTCAAGGGGCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCC790.10024235810630765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGT307.859071E-752.03875746
ATCCCGC203.4153627E-452.03875744
ATCCCCT203.4153627E-452.03875748
AAACCTC203.4153627E-452.03875746
TGGCAGA150.007174137552.03875743
GTGTTGA150.007174137552.03875748
CATGAAA150.007174137552.03875752
GTCATGA150.007174137552.03875750
TGCGCCC150.007174137552.03875745
CGCCTGA150.007174137552.03875746
GCGATGA203.4153627E-452.03875749
GATACAA150.007174137552.03875751
GTCGCGT203.4153627E-452.03875744
TAAAATC203.4153627E-452.03875750
CACAAGA150.007174137552.03875743
TCGCGTG203.4153627E-452.03875745
GATCGCG150.007174137552.03875745
CGAAGGG203.4153627E-452.03875752
CTCTATA150.007174137552.03875749
CTGATAC150.007174137552.03875749
Produced by FastQC (version 0.11.5)