FastQCFastQC Report
Tue 31 May 2016
SRR212496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78669
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9991.269877588376616No Hit
GCTCAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9371.1910663666755648No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8141.034715072010576No Hit
GCTCAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5500.6991318054125513No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5300.6737088306702766No Hit
GCTCAAGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4770.606337947603249No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4550.578372675386747No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3920.498290304948582No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACTGTAGCGCAGGCCGCTC3370.4283771244073269No Hit
GCTCAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3150.40041185219082487No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2910.3699042825000953No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2870.3648196875516404No Hit
GCTCAAGGGGTGCAGTGCTGCTCAAGGGCACAACCTATCTCAGAAGTTGTAGATGAAG2820.3584639438660718No Hit
GCTCAAGGGGCCCTGGCGGCCCGGAGCCGCGCTCGTTGGGCTCCCATCCTGGGTCCCG2640.3355832665980246No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2600.3304986716495697No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2420.3076179943815226No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2400.30507569690729514No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG2380.3025333994330677No Hit
GCTCAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2310.29363535827327153No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2160.2745681272165656No Hit
GCTCAAGGGGGCTAGTCCTTCTTAAAGCTCTAGCCAGTGGGAAAGCGTTTGTGTTTCT2040.2593143423712009No Hit
GCTCAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2010.2555008961598597No Hit
GCTCAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1890.2402471113144949No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.22372217773201641No Hit
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1670.21228183909799284No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGA1660.2110106903608791No Hit
GCTCAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1640.20846839288665167No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1640.20846839288665167No Hit
GCTCAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA1580.2008415004639693No Hit
GCTCAAGGGGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTGCT1560.19829920298974182No Hit
GCTCAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1520.1932146080412869No Hit
GCTCAAGGGGAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGCG1480.18813001309283198No Hit
GCTCAAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT1440.18304541814437708No Hit
GCTCAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1400.17796082319592216No Hit
GCTCAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC1400.17796082319592216No Hit
GCTCAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1370.17414737698458096No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC1360.17287622824746723No Hit
GCTCAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1350.1716050795103535No Hit
GCTCAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1350.1716050795103535No Hit
GCTCAAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT1310.16652048456189858No Hit
GCTCAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1290.16397818708767115No Hit
GCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1280.16270703835055741No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACTGTAGCGCAGGCCGCTC1270.16143588961344366No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1260.16016474087632993No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1220.155080145927875No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1220.155080145927875No Hit
GCTCAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1200.15253784845364757No Hit
GCTCAAGGGATGTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGAAG1190.15126669971653384No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.15126669971653384No Hit
GCTCAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1160.14745325350519264No Hit
GCTCAAGGGAGTGAAGTGGGGAAAATTAGCACCCTAAATCTTGTGATCTACACAGGTG1150.1461821047680789No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAA1110.141097509819624No Hit
GCTCAAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1080.1372840636082828No Hit
GCTCAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT1080.1372840636082828No Hit
GCTCAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1080.1372840636082828No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG1070.1360129148711691No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG1070.1360129148711691No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1050.1334706173969416No Hit
GCTCAAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA1030.13092831992271417No Hit
GCTCAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG990.12584372497425922No Hit
GCTCAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC980.1245725762371455No Hit
GCTCAAGGGGCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTCCTC960.12203027876291805No Hit
GCTCAAGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT950.12075913002580432No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA940.11948798128869058No Hit
GCTCAAGGGAGACCAGAAATATTTTATTGTTCTTGGAATCATATTTTTGTGCTTCCAT940.11948798128869058No Hit
GCTCAAGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCTGGTATT940.11948798128869058No Hit
GCTCAAGGGGGCCATAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAA930.11821683255157686No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG930.11821683255157686No Hit
GCTCAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC910.1156745350773494No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCCAA900.11440338634023568No Hit
GCTCAAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCCTCG900.11440338634023568No Hit
GCTCAAGGGGCCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATC900.11440338634023568No Hit
GCTCAAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCACGC900.11440338634023568No Hit
GCTCAAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG890.11313223760312194No Hit
GCTCAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG890.11313223760312194No Hit
GCTCAAGGGGCCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA860.10931879139178076No Hit
GCTCAAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG860.10931879139178076No Hit
GCTCAAGGGCTTCTTCTAGGCTCCGAGCGGTTGGTTTCTTGGGCCCTGTCTGTAGGCC840.10677649391755328No Hit
GCTCAAGGGGCCTTTCCTTTCAGCGGCGCGCGGCTGCAAGATGGCGGTGCAGATTTCC840.10677649391755328No Hit
GCTCAAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT830.10550534518043955No Hit
GCTCAAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTCGA820.10423419644332584No Hit
GCTCAAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGGGC820.10423419644332584No Hit
GCTCAAGGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG820.10423419644332584No Hit
GCTCAAGGGGCTCTTTCCGTCTCCGGCCGCCATAGGGAGAGGAGCCGCCGCCATGTCC810.1029630477062121No Hit
GCTCAAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG810.1029630477062121No Hit
GCTCAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC800.10169189896909837No Hit
GCTCAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA800.10169189896909837No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA800.10169189896909837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG251.6297186E-552.0605526
CAGTAGC251.6297186E-552.0605527
GCCAGTA251.6297186E-552.0605525
ATTAAGC251.6297186E-552.0605551
ACTGGCT1100.052.0605524
GTAGATG150.007162187752.06054749
ATCCTAA203.408245E-452.06054725
CCAATTC203.408245E-452.06054752
AACAAGC203.408245E-452.06054750
GATACAA150.007162187752.06054751
CGCCTCC150.007162187752.06054724
CGCCCAG150.007162187752.06054752
ATTAGCA150.007162187752.06054725
AGGATGC150.007162187752.06054752
GGCCGCT850.052.06054751
TCGGCAC203.408245E-452.06054727
ACCAATT203.408245E-452.06054751
CGAAGGG150.007162187752.06054752
CTGATAC150.007162187752.06054749
GAGTTCA203.408245E-452.06054752
Produced by FastQC (version 0.11.5)