FastQCFastQC Report
Tue 31 May 2016
SRR212495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77881
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8931.1466211271041717No Hit
GCTCAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8261.0605924423158408No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6940.8911030931806218No Hit
GCTCAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5240.672821355657991No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4840.6214609468291368No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4530.5816566299867747No Hit
GCTCAAGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4320.5546924153516262No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACTGTAGCGCAGGCCGCTC3720.4776518021083448No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3530.453255607914639No Hit
GCTCAAGGGGCCCTGGCGGCCCGGAGCCGCGCTCGTTGGGCTCCCATCCTGGGTCCCG2730.3505347902569304No Hit
GCTCAAGGGGTGCAGTGCTGCTCAAGGGCACAACCTATCTCAGAAGTTGTAGATGAAG2660.3415467187118809No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2590.3325586471668314No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2580.3312746369461101No Hit
GCTCAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2560.32870661650466737No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2400.30816245297312567No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2370.3043104223109616No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2180.2799142281172558No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2020.2593700645857141No Hit
GCTCAAGGGGGCTAGTCCTTCTTAAAGCTCTAGCCAGTGGGAAAGCGTTTGTGTTTCT1930.2478139725992219No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG1910.2452459521577792No Hit
GCTCAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1750.22470178862623746No Hit
GCTCAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1740.2234177784055161No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGA1710.21956574774335205No Hit
GCTCAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1680.21571371708118797No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1650.21186168641902392No Hit
GCTCAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1590.20415762509469576No Hit
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1560.20030559443253168No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.19902158421181032No Hit
GCTCAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1500.19260153310820355No Hit
GCTCAAGGGGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTGCT1380.17719341045954726No Hit
GCTCAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1300.1669213286937764No Hit
GCTCAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1290.16563731847305505No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC1280.16435330825233369No Hit
GCTCAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA1270.16306929803161235No Hit
GCTCAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1260.16178528781089097No Hit
GCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1250.16050127759016963No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1230.1579332571487269No Hit
GCTCAAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1220.15664924692800555No Hit
GCTCAAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT1210.1553652367072842No Hit
GCTCAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1190.15279721626584147No Hit
GCTCAAGGGATGTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGAAG1160.1489451856036774No Hit
GCTCAAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT1160.1489451856036774No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1110.14252513450007062No Hit
GCTCAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC1100.14124112427934926No Hit
GCTCAAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA1100.14124112427934926No Hit
GCTCAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1080.13867310383790654No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACTGTAGCGCAGGCCGCTC1070.13738909361718518No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG1070.13738909361718518No Hit
GCTCAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1040.13353706295502113No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAA1030.13225305273429977No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG1020.1309690425135784No Hit
GCTCAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1020.1309690425135784No Hit
GCTCAAGGGGAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGCG1000.1284010220721357No Hit
GCTCAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT990.12711701185141436No Hit
GCTCAAGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCTGGTATT980.12583300163069297No Hit
GCTCAAGGGAGTGAAGTGGGGAAAATTAGCACCCTAAATCTTGTGATCTACACAGGTG970.12454899140997162No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA960.12326498118925026No Hit
GCTCAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC930.1194129505270862No Hit
GCTCAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC920.11812894030636482No Hit
GCTCAAGGGGCCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATC910.11684493008564348No Hit
GCTCAAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAAAG910.11684493008564348No Hit
GCTCAAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGTGC890.11427690964420077No Hit
GCTCAAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG880.11299289942347943No Hit
GCTCAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC860.1104248789820367No Hit
GCTCAAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCCTCG850.10914086876131533No Hit
GCTCAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC850.10914086876131533No Hit
GCTCAAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG840.10785685854059399No Hit
GCTCAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG840.10785685854059399No Hit
GCTCAAGGGGGCCATAGTGGGTGATAGCCCTGTACGCGAAAATCTCTTAGTCATGAAA840.10785685854059399No Hit
GCTCAAGGGGCTCTTTCCGTCTCCGGCCGCCATAGGGAGAGGAGCCGCCGCCATGTCC830.10657284831987263No Hit
GCTCAAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCACGC830.10657284831987263No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.10657284831987263No Hit
GCTCAAGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT820.10528883809915127No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA810.1040048278784299No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA810.1040048278784299No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG800.10272081765770856No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG800.10272081765770856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAT150.00704487752.277679
AGGGCGT150.00704487752.277676
AGGGCCT458.54925E-1152.277676
GGATTCT307.6127253E-752.277678
GCAGTCA150.00704487752.277679
TATCCAC150.00704487752.2776710
GATCCAC150.00704487752.277679
AAGCGTG150.00704487752.2776710
GGGGATA307.6127253E-752.277677
AGGGGGA2300.052.2776686
GTACCTG203.3386162E-452.2776689
GGGTACC251.5898382E-552.2776687
GGACTAA251.5898382E-552.2776688
AGGGTCT203.3386162E-452.2776686
AGGGTAC401.7698767E-952.2776686
GGATCCA353.6634447E-852.2776688
AGGGACT1150.052.2776686
CGGGTGT203.3386162E-452.27766810
GGTAGTT203.3386162E-452.2776688
GGGACCA203.3386162E-452.2776687
Produced by FastQC (version 0.11.5)