SRR212494_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212494_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77370
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1291	1.6686054026108312	No Hit
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	970	1.2537159105596485	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	874	1.1296368101331267	No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	856	1.1063719788031536	No Hit
GCTCAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	812	1.0495023911076644	No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	474	0.6126405583559519	No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	397	0.5131187798888458	No Hit
GCTCAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	391	0.5053638361121882	No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	368	0.4756365516350006	No Hit
GCTCAAGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC	363	0.46917409848778596	No Hit
GCTCAAGGGGTGCAGTGCTGCTCAAGGGCACAACCTATCTCAGAAGTTGTAGATGA	346	0.44720175778725607	No Hit
GCTCAAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	263	0.3399250355434923	No Hit
GCTCAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	233	0.3011503166602042	No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	229	0.29598035414243246	No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACTGTAGCGCAGGCCGC	221	0.28564042910688897	No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	213	0.2753005040713455	No Hit
GCTCAAGGGGCCCTGGCGGCCCGGAGCCGCGCTCGTTGGGCTCCCATCCTGGGTCC	212	0.27400801344190256	No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	201	0.2597906165180302	No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	192	0.2481582008530438	No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	188	0.24298823833527206	No Hit
GCTCAAGGGGGCTAGTCCTTCTTAAAGCTCTAGCCAGTGGGAAAGCGTTTGTGTTT	181	0.23394080392917152	No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	179	0.23135582267028565	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	174	0.22489336952307096	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	174	0.22489336952307096	No Hit
GCTCAAGGGATTCTCGCAAGGAAAACATTGCCACCATTTGTAGAACATGTAATCTT	172	0.2223083882641851	No Hit
GCTCAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC	169	0.2184309163758563	No Hit
GCTCAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	167	0.21584593511697042	No Hit
GCTCAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA	164	0.2119684632286416	No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.19775106630476927	No Hit
GCTCAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	143	0.1848261600103399	No Hit
GCTCAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT	136	0.17577872560423938	No Hit
GCTCAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG	134	0.1731937443453535	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC	133	0.17190125371591056	No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATC	133	0.17190125371591056	No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	127	0.16414630993925294	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	125	0.16156132868036707	No Hit
GCTCAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	125	0.16156132868036707	No Hit
GCTCAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC	122	0.15768385679203825	No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	120	0.15509887553315238	No Hit
GCTCAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	120	0.15509887553315238	No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	119	0.15380638490370943	No Hit
GCTCAAGGGGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTG	115	0.1486364223859377	No Hit
GCTCAAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA	114	0.14734393175649477	No Hit
GCTCAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA	112	0.1447589504976089	No Hit
GCTCAAGGGATGTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGA	111	0.14346645986816595	No Hit
GCTCAAGGGAGACCAGAAATATTTTATTGTTCTTGGAATCATATTTTTGTGCTTCC	106	0.13700400672095128	No Hit
GCTCAAGGGAGTGAAGTGGGGAAAATTAGCACCCTAAATCTTGTGATCTACACAGG	104	0.1344190254620654	No Hit
GCTCAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT	103	0.13312653483262246	No Hit
GCTCAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGA	102	0.13183404420317954	No Hit
GCTCAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	100	0.12924906294429364	No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	98	0.12666408168540777	No Hit
GCTCAAGGGGAAGCCAGCTTGGAGCACAGTGAGATGCTGAGGAATACCAGCCTCCA	94	0.12149411916763603	No Hit
GCTCAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	93	0.12020162853819309	No Hit
GCTCAAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG	93	0.12020162853819309	No Hit
GCTCAAGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGGTCTGGGA	93	0.12020162853819309	No Hit
GCTCAAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC	92	0.11890913790875016	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC	88	0.11373917539097841	No Hit
GCTCAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAG	88	0.11373917539097841	No Hit
GCTCAAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCG	87	0.11244668476153548	No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGT	86	0.11115419413209254	No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	86	0.11115419413209254	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCC	82	0.1059842316143208	No Hit
GCTCAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG	82	0.1059842316143208	No Hit
GCTCAAGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	82	0.1059842316143208	No Hit
GCTCAAGGGGGGGAGTAAAGAGTTCTACAAAAGGTAGCTGTGAAAATATACATACA	82	0.1059842316143208	No Hit
GCTCAAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC	81	0.10469174098487787	No Hit
GCTCAAGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	80	0.10339925035543493	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	80	0.10339925035543493	No Hit
GCTCAAGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACT	80	0.10339925035543493	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAAGC	25	2.0002364E-5	50.28702	50
CTGATAC	25	2.0002364E-5	50.28702	49
TGATACA	25	2.0002364E-5	50.28702	50
ACATCGA	40	2.4974725E-9	50.28702	50
ACACAGG	20	4.0434883E-4	50.28702	50
TATTATA	20	4.0434883E-4	50.28702	49
AGATTAA	25	2.0002364E-5	50.28702	49
AACGCAC	15	0.008213273	50.287018	49
AAGTACA	15	0.008213273	50.287018	49
TAAGTGT	15	0.008213273	50.287018	50
ACGCACC	15	0.008213273	50.287018	50
TTATTTG	15	0.008213273	50.287018	50
TAATCTT	15	0.008213273	50.287018	50
GCGCCAG	15	0.008213273	50.287018	49
TTAAGCT	15	0.008213273	50.287018	49
TCTCAGG	15	0.008213273	50.287018	49
GTAATCT	15	0.008213273	50.287018	49
TAGGCTA	15	0.008213273	50.287018	49
AAATTCG	25	2.0079793E-5	50.254242	48
CCGTAAA	25	2.0079793E-5	50.254242	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content