##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77370 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.85516350006463 39.0 38.0 40.0 33.0 40.0 2 37.45375468527853 39.0 38.0 40.0 33.0 40.0 3 37.34915341863771 39.0 37.0 40.0 33.0 40.0 4 37.73834819697557 39.0 38.0 40.0 33.0 40.0 5 37.63536254362156 39.0 38.0 40.0 33.0 40.0 6 37.897647667054414 39.0 38.0 40.0 35.0 40.0 7 38.10802636680884 39.0 38.0 40.0 35.0 40.0 8 38.124402223083884 39.0 38.0 40.0 35.0 40.0 9 38.08238335272069 39.0 38.0 40.0 35.0 40.0 10 37.246490887941064 39.0 37.0 40.0 33.0 40.0 11 37.18525268191806 39.0 37.0 40.0 33.0 40.0 12 37.10373529791909 39.0 36.0 40.0 32.0 40.0 13 37.06692516479256 39.0 36.0 40.0 32.0 40.0 14 37.0454827452501 39.0 36.0 40.0 32.0 40.0 15 37.238477446038516 39.0 37.0 40.0 33.0 40.0 16 37.09992245056223 39.0 36.0 40.0 33.0 40.0 17 36.68954375080781 38.0 36.0 40.0 31.0 40.0 18 36.617035026496055 38.0 36.0 40.0 31.0 40.0 19 36.49398991857309 38.0 35.0 40.0 31.0 40.0 20 36.44769290422644 38.0 35.0 40.0 31.0 40.0 21 36.31796561974926 38.0 35.0 40.0 31.0 40.0 22 36.114618069019 38.0 35.0 40.0 30.0 40.0 23 35.85018741114127 38.0 35.0 40.0 29.0 40.0 24 35.77367196587825 38.0 35.0 40.0 29.0 40.0 25 35.728499418379215 38.0 35.0 39.0 29.0 40.0 26 35.19848778596355 38.0 35.0 39.0 28.0 40.0 27 35.034587049243896 38.0 34.0 39.0 28.0 40.0 28 34.672353625436216 38.0 33.0 39.0 27.0 40.0 29 34.58323639653612 38.0 33.0 39.0 27.0 40.0 30 34.07683856792038 37.0 33.0 39.0 25.0 40.0 31 33.385252681918054 37.0 32.0 39.0 23.0 40.0 32 33.40073671965878 37.0 33.0 39.0 23.0 40.0 33 32.99287837663177 36.0 32.0 39.0 21.0 40.0 34 33.190409719529534 36.0 32.0 39.0 23.0 40.0 35 32.818598940157685 36.0 32.0 39.0 21.0 40.0 36 32.74125630089182 37.0 32.0 39.0 18.0 40.0 37 32.44788677782086 36.0 32.0 39.0 16.0 40.0 38 32.05093705570635 36.0 31.0 39.0 13.0 40.0 39 31.911684115290164 36.0 31.0 39.0 10.0 40.0 40 31.790887941062426 36.0 31.0 39.0 6.0 40.0 41 31.108892335530566 36.0 30.0 39.0 2.0 40.0 42 30.782396277626987 36.0 30.0 39.0 2.0 40.0 43 30.94672353625436 36.0 30.0 39.0 2.0 40.0 44 30.74192839601913 36.0 30.0 39.0 2.0 40.0 45 29.980056869587695 35.0 29.0 39.0 2.0 40.0 46 29.5436215587437 35.0 28.0 38.0 2.0 40.0 47 29.234444875274654 35.0 28.0 38.0 2.0 40.0 48 29.024789970272714 35.0 27.0 38.0 2.0 40.0 49 28.608323639653612 35.0 26.0 38.0 2.0 40.0 50 28.200529921158072 34.0 25.0 38.0 2.0 40.0 51 27.44120460126664 34.0 23.0 38.0 2.0 40.0 52 27.31441127051829 34.0 23.0 38.0 2.0 40.0 53 26.837068631252425 33.0 19.0 38.0 2.0 39.0 54 26.472185601654388 33.0 15.0 38.0 2.0 39.0 55 26.041372625048467 33.0 2.0 38.0 2.0 39.0 56 15.062401447589504 19.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.9082681723996942 1 2 -2.0441381522120707 1 3 -2.984102953842779 1 4 -2.4387021771041333 1 5 -1.6029209603419332 1 6 -2.0423045005169342 1 7 -3.261629275715478 1 8 -2.759526181802343 1 9 -2.6012592572521314 1 10 -2.488067428770627 1 11 -2.3363297821757314 1 12 -2.6870582251790935 1 13 -2.9218580866234447 1 14 -2.7959661808791836 1 15 -2.653213196326888 1 16 -2.3572977232571617 1 17 -2.6919520673137143 1 18 -1.5776694118948313 1 19 -1.813497542775437 1 20 -1.783770836333062 1 21 -2.275804073425242 1 22 -2.676904656178394 1 23 -2.5695700597145077 1 24 -3.7668950288518204 1 25 -3.3954397078085776 1 26 -3.9716578619099856 1 27 -1.8752437822031425 1 28 -2.5208004257396794 1 29 -3.7056940354180057 1 30 -2.6899778188259944 1 31 -1.4376076745237079 1 32 -1.6578776728680893 1 33 -1.367037853427302 1 34 -0.9773623641164875 1 35 -1.7328798728984687 1 36 -1.1967251129742031 1 37 -1.4622234993133496 1 38 -1.6078238834467342 1 39 -0.8882477840806686 1 40 -0.28369340759243755 1 41 -1.52923930322455 1 42 -1.2100577993988786 1 43 -0.7674595918757063 1 44 -1.013596962381765 1 45 -1.1706547153019784 1 46 -1.5485028469336157 1 47 -1.883813771448672 1 48 -1.2555787254987436 1 49 0.24938782856690267 1 50 0.29153301723270886 1 51 -0.7732083415124684 1 52 0.4606831881789333 1 53 0.3503320222064197 1 54 -1.3172516217091612 1 55 -0.4111890015054236 1 56 -0.5372541007492142 2 1 -0.4508607649922851 2 2 -0.4959900040639269 2 3 -1.7489177686575985 2 4 -0.6109243993263576 2 5 -0.9344024418234156 2 6 -0.4404526486650866 2 7 -0.45977742386362763 2 8 -0.478044700320865 2 9 -0.49570370169657707 2 10 -0.5065859472891461 2 11 -0.5826260784720318 2 12 -0.009280447401309289 2 13 0.19851228374692909 2 14 0.36699678208378117 2 15 0.2727127295990357 2 16 -0.9461866121460503 2 17 0.04138126601962 2 18 -1.0165583007837213 2 19 -0.7968308761087712 2 20 -0.2782152807775091 2 21 -0.05728555490671994 2 22 0.33235460308086573 2 23 0.5952447551003104 2 24 0.12199386003707247 2 25 0.10085658848772283 2 26 -0.7957319359840582 2 27 -1.427095634055 2 28 -0.6319115368507937 2 29 -1.164953294677268 2 30 -0.7992370780852553 2 31 -0.34316323007926286 2 32 -0.5115813765717903 2 33 -0.4170378534273027 2 34 -0.8681031048572265 2 35 -0.9402872803058742 2 36 0.6588304425813547 2 37 -0.2918531289429822 2 38 0.4218057461828977 2 39 0.3561966603637785 2 40 -0.25776748166650876 2 41 0.9281681041828591 2 42 1.0325347931937152 2 43 1.093651519235408 2 44 0.5697363709515706 2 45 1.2052712106239483 2 46 0.6607564123256466 2 47 0.07359363595873347 2 48 0.899976830056815 2 49 1.6771656063446798 2 50 1.5248663505660396 2 51 1.8064212881171642 2 52 1.812535040030788 2 53 2.087369059243457 2 54 2.245711341253802 2 55 2.385107294790874 2 56 2.3571903436952297 3 1 -0.1523759165074381 3 2 -0.8959900040639255 3 3 -0.46558443532425997 3 4 -0.07001530841726833 3 5 0.23377937635839885 3 6 -0.7510587092711418 3 7 -0.318868332954537 3 8 -0.3083477306238933 3 9 -0.3335824895753632 3 10 -0.46113140183459933 3 11 0.04919210334615087 3 12 -0.34867438679525264 3 13 -0.12724529201064882 3 14 0.500330115417114 3 15 -0.18183272494641756 3 16 -0.8522472182066565 3 17 -0.9343763097379565 3 18 -1.0120128462382638 3 19 -0.38925511853300776 3 20 -0.6070031595653873 3 21 -0.36334616096733185 3 22 0.052051572777834565 3 23 0.7906993005548557 3 24 0.12199386003707247 3 25 0.2947959824271109 3 26 0.8633589731068483 3 27 0.22744982049045603 3 28 0.4589975540582998 3 29 -0.8891957189196873 3 30 -0.34317647202464485 3 31 0.2189579820419496 3 32 0.5172065022160837 3 33 0.2693257829363347 3 34 0.7212908345367097 3 35 -0.45846909848769 3 36 0.661860745611655 3 37 0.4232983862085362 3 38 0.29453301891016537 3 39 0.5410451452122587 3 40 1.1422325183334898 3 41 0.3630165890313428 3 42 -0.7992833886244668 3 43 1.0254697010535878 3 44 0.38034243155762937 3 45 0.8310287863815233 3 46 0.9986352002044327 3 47 0.12965424201933828 3 48 1.0424010724810557 3 49 1.0726201517992244 3 50 1.624866350566041 3 51 1.4624818941777669 3 52 1.5852623127580578 3 53 1.0388842107586065 3 54 2.918438613981074 3 55 1.6426830523666318 3 56 1.4071903436952304 4 1 -0.3751777048829936 4 2 -0.5435309876704864 4 3 -0.6431800637395639 4 4 -0.5106511752826393 4 5 -0.3193751194190426 4 6 -1.0046602989383118 4 7 -1.1092309757761996 4 8 -0.9310501648017393 4 9 -0.6194741934998547 4 10 -1.9109575319886005 4 11 -0.7719703407671119 4 12 -0.8300454747237183 4 13 -1.1498483719907782 4 14 -0.8002163326703169 4 15 -1.8010577622042447 4 16 -1.3909953553154466 4 17 -1.2493291164940388 4 18 -1.305356114991369 4 19 -1.0867215864912794 4 20 -2.296521291706469 4 21 -1.8966298172018057 4 22 -1.45862900347651 4 23 -1.754755244899691 4 24 -1.8424870142798682 4 25 -2.238487673807363 4 26 -1.6195024277873387 4 27 -2.120538257005819 4 28 -2.4024033401294815 4 29 -3.0223303438575932 4 30 -2.818089537101649 4 31 -1.1297752519371898 4 32 -1.1648600650963843 4 33 -1.1812455037005272 4 34 -1.464004744201489 4 35 -1.2495768977922133 4 36 -1.2996395027738394 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0.5041392248432501 6 6 -0.0446193153317509 6 7 0.2985559094697052 6 8 0.16987196634580215 6 9 0.06367129830342577 6 10 -0.9701276139558104 6 11 -0.210751078472029 6 12 0.45530288593202073 6 13 0.4485122837469291 6 14 0.47949678208377833 6 15 -0.3054122704009643 6 16 -0.2513949454793831 6 17 -1.6294520673137143 6 18 -0.5550999674503885 6 19 0.019835790557898747 6 20 -0.30113194744417626 6 21 0.16458944509327722 6 22 -0.01139539691913427 6 23 -0.25475524489969104 6 24 0.9136605267037368 6 25 0.29148158848772 6 26 1.2511430640159418 6 27 0.7729043659450028 6 28 0.8524634631492063 6 29 1.7537967053227348 6 30 1.160137921914746 6 31 1.7047534365874029 6 32 1.7790436234282083 6 33 0.45067047990603015 6 34 1.735021895142772 6 35 2.1305460530274587 6 36 2.535913775914686 6 37 2.6112718710570206 6 38 2.7457640795162277 6 39 3.10827999369711 6 40 3.06410751833349 6 41 3.6021264375161905 6 42 2.189826459860381 6 43 3.4009431859020722 6 44 3.5749447042849027 6 45 2.0000628772906133 6 46 2.0336730789923116 6 47 1.7996353026254006 6 48 2.2062268300568135 6 49 2.4698739396780134 6 50 1.093616350566041 6 51 0.5074629547838292 6 52 0.5364933733641202 6 53 1.11132739257679 6 54 1.1665446745871364 6 55 0.322607294790874 6 56 -1.3584346563047696 7 1 -0.46105976996740594 7 2 -0.4407661234669078 7 3 -1.5227983656725215 7 4 -0.807690568480588 7 5 -0.7908701035149619 7 6 -0.7377163302571219 7 7 -0.36002618008253506 7 8 -0.3651093769377809 7 9 -0.4039126569204541 7 10 -0.9045958975379023 7 11 -0.14755145160635408 7 12 0.2822618411559006 7 13 0.14254213449319764 7 14 0.48042961790467587 7 15 0.04136944601694381 7 16 -0.39295278130028066 7 17 -1.1397132613435659 7 18 -0.42426974356979485 7 19 -0.5398657019794157 7 20 -0.03667299222029641 7 21 -1.2722109280410479 7 22 -0.4698842028892827 7 23 -0.6502776329593942 7 24 -0.6367126076246237 7 25 -0.6439195309152623 7 26 0.47665612371743293 7 27 0.9743969032584374 7 28 1.084506373596966 7 29 0.34101685457646624 7 30 0.797031578631163 7 31 0.38992134703516257 7 32 0.33991116074164296 7 33 0.6827133903537899 7 34 0.5878670443965035 7 35 0.8796132172065612 7 36 0.6996264624818522 7 37 0.7365050800122432 7 38 0.7506614675759309 7 39 0.00893297877173893 7 40 1.1123817720648326 7 41 1.5622477061729043 7 42 0.27308205687530673 7 43 -0.13893554544121045 7 44 0.8396368684640088 7 45 0.9517886235592705 7 46 -0.17481572697783676 7 47 1.2723498548642063 7 48 1.173111158415022 7 49 0.22617058146905933 7 50 0.7591947087749951 7 51 0.8962222831420377 7 52 1.3324355375432262 7 53 1.175926273173804 7 54 1.1656118387662424 7 55 0.28809236941773975 7 56 1.0713694481728417 8 1 -0.646694098325618 8 2 0.16650999593607452 8 3 0.18545723134240433 8 4 0.4276172673403096 8 5 -0.16773577515674987 8 6 -0.1852443153317509 8 7 0.11105590946970523 8 8 -0.12700303365419785 8 9 0.0011712983034257718 8 10 0.13924738604418962 8 11 -0.195126078472029 8 12 -0.24782211406797927 8 13 -0.4264877162530709 8 14 0.07324678208377833 8 15 -0.0554122704009643 8 16 0.2642300545206169 8 17 -0.20757706731371428 8 18 0.3980250325496115 8 19 0.06671079055789875 8 20 1.0426180525558237 8 21 0.00833944509327722 8 22 0.5354796030808657 8 23 0.35461975510030896 8 24 0.6480355267037368 8 25 0.26023158848772 8 26 1.1730180640159418 8 27 0.7104043659450028 8 28 0.2587134631492063 8 29 1.3319217053227348 8 30 0.6913879219147461 8 31 1.4391284365874029 8 32 1.4977936234282083 8 33 1.9819204799060302 8 34 1.110021895142772 8 35 2.1930460530274587 8 36 1.6296637759146861 8 37 1.6581468710570206 8 38 1.7145140795162277 8 39 2.35827999369711 8 40 1.3141075183334898 8 41 3.1802514375161905 8 42 2.283576459860381 8 43 2.3853181859020722 8 44 2.8718197042849027 8 45 1.2656878772906133 8 46 2.2211730789923116 8 47 2.1433853026254006 8 48 1.2999768300568135 8 49 2.3136239396780134 8 50 2.171741350566041 8 51 1.4293379547838292 8 52 1.3177433733641202 8 53 1.48632739257679 8 54 0.9477946745871364 8 55 0.775732294790874 8 56 -0.04593465630476956 9 1 -0.3726556367871581 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120 30 -1.8915447703929473 120 31 4.2651351048306196E-4 120 32 -0.2846582996487115 120 33 -0.36062759701705005 120 34 0.33958920283507865 120 35 -0.1146462546648479 120 36 1.365240698991606 120 37 1.3696853325954805 120 38 1.8551390795162277 120 39 1.7356838398509566 120 40 0.7383863644873365 120 41 2.0768860529008037 120 42 1.7222783829373007 120 43 1.3180104935943788 120 44 1.306915858131056 120 45 1.3257840311367666 120 46 0.12742307899231164 120 47 -0.2712781589130593 120 48 0.7807460608260435 120 49 0.6489604781395535 120 50 0.4133278890275811 120 51 -0.1307581990623241 120 52 -0.10413162663587983 120 53 0.39377931565371327 120 54 0.20981390535636635 120 55 -0.5379696282860493 120 56 1.0994980360029238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49.0 3 6.0 4 19.0 5 29.0 6 27.0 7 58.0 8 65.0 9 106.0 10 153.0 11 124.0 12 180.0 13 228.0 14 326.0 15 359.0 16 460.0 17 521.0 18 568.0 19 623.0 20 775.0 21 813.0 22 811.0 23 1067.0 24 1407.0 25 1457.0 26 1430.0 27 1437.0 28 1643.0 29 1855.0 30 2320.0 31 2670.0 32 3114.0 33 3885.0 34 5059.0 35 6590.0 36 8664.0 37 11111.0 38 11274.0 39 6087.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 65.3638361121882 21.620783249321445 3.569859118521391 9.445521519968981 11 33.34108827710999 19.671707380121497 15.767093188574382 31.22011115419413 12 36.803670673387614 10.301150316660204 33.183404420317956 19.711774589634228 13 23.324285898927233 13.223471629830682 30.288225410365776 33.16401706087631 14 17.358149153418637 19.87204342768515 42.87837663176942 19.891430787126794 15 24.092025332816334 15.076903192451855 39.37960449786739 21.451466976864417 16 25.980354142432464 14.685278531730644 36.01783637068631 23.316530955150576 17 28.73982163629314 14.839084916634354 26.877342639265866 29.543750807806646 18 30.798759208995737 16.872172676748093 24.92955926069536 27.39950885356081 19 26.99366679591573 23.179526948429626 25.619749256817887 24.20705699883676 20 29.20253328163371 21.45275946749386 27.674809357632157 21.669897893240275 21 28.322347162983068 21.31963293266124 25.879539873335915 24.478480031019775 22 27.978544655551246 20.51958123303606 28.190513118779887 23.311360992632803 23 31.207186247899703 22.051182628925943 24.81065012278661 21.930981000387746 24 31.279565723148504 21.6556804963164 25.552539744086854 21.512214036448235 25 28.751696942271636 22.79138923007305 19.587562221216626 28.86935160643868 26 28.499928869805878 20.06776767585324 25.60945643598929 25.82284701835159 27 24.121376811594203 16.66149068322981 24.205486542443065 35.01164596273292 28 24.74924612059482 25.25787205404636 22.215175948334995 27.777705877023827 29 30.637978354305833 20.07534565791518 23.802495986743306 25.48418000103568 30 26.50951949229374 22.352026939515607 30.13081207097526 21.00764149721539 31 31.553247156956715 19.261456363495068 28.742584773204154 20.442711706344067 32 32.69228277068619 16.3856670770427 28.761744314132052 22.160305838139053 33 34.760225121249064 15.890759135824883 27.569572321498043 21.779443421428017 34 25.990920881971462 20.52788586251621 33.09338521400778 20.38780804150454 35 24.10929755819083 23.386615460466565 35.73967875029192 16.76440823105068 36 27.272845275181723 28.857736240913813 27.7466251298027 16.122793354101766 37 30.886381636177145 22.55878266966152 28.287090533750536 18.267745160410797 38 24.212987012987014 26.10909090909091 28.380519480519478 21.2974025974026 39 29.137069021492113 20.97136549574703 29.926628141029806 19.964937341731055 40 28.502936437815084 22.326022556000208 26.495504391663633 22.675536614521075 41 25.05391991268872 23.110204505885715 29.201205727204222 22.634669854221347 42 20.426373226154706 22.001534839557237 32.43454169430679 25.13755023998127 43 25.625251986630076 18.353730702701295 27.586520828727124 28.4344964819415 44 23.782644886068045 17.850639891790657 29.463635417750496 28.903079804390803 45 25.18836619168456 21.745071247316027 29.17821588912746 23.88834667187195 46 25.548860634296794 23.177730638591377 26.707096471935554 24.566312255176275 47 22.568802562032964 20.255422188663523 30.20152576353269 26.974249485770823 48 21.95144189439133 19.414124573423294 32.4732331257978 26.16120040638758 49 23.99864620351206 21.143958032308873 31.258379870087605 23.599015894091462 50 22.668489939441297 20.467539232923095 33.98189750602331 22.882073321612296 51 24.470287939364734 20.128407152252333 34.20500866032011 21.196296248062822 52 23.364388559515277 20.953808065672032 31.420939474884356 24.260863899928335 53 18.607378833096583 22.56134909361031 36.08095604238073 22.750316030912384 54 20.096997509875756 22.671864203486177 35.11466305098888 22.11647523564919 55 21.433133056160152 20.284906225969998 31.738501440170996 26.543459277698854 56 20.162919517758226 23.54512870641903 27.804496578690124 28.487455197132615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 15.0 11 30.0 12 58.5 13 76.5 14 66.0 15 93.0 16 120.0 17 239.0 18 358.0 19 396.0 20 434.0 21 415.5 22 514.0 23 631.0 24 805.5 25 980.0 26 1174.0 27 1368.0 28 1547.0 29 1787.0 30 1848.0 31 1770.0 32 1692.0 33 1581.5 34 1471.0 35 1819.0 36 2167.0 37 1654.5 38 1027.0 39 912.0 40 1154.0 41 1396.0 42 1305.0 43 1214.0 44 1427.5 45 1641.0 46 1937.5 47 1955.0 48 1676.0 49 1759.0 50 1842.0 51 2296.0 52 2750.0 53 3095.0 54 3492.0 55 3544.0 56 3723.0 57 3902.0 58 5022.5 59 6143.0 60 6599.0 61 7055.0 62 7336.5 63 6551.0 64 5484.0 65 4950.0 66 4416.0 67 3607.5 68 2799.0 69 2213.0 70 1627.0 71 1278.5 72 896.5 73 863.0 74 637.0 75 411.0 76 451.5 77 492.0 78 344.5 79 156.0 80 115.0 81 95.5 82 76.0 83 58.0 84 40.0 85 30.0 86 20.0 87 15.0 88 7.0 89 4.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03231226573607341 26 0.060747059583818014 27 0.11632415664986429 28 0.13312653483262246 29 0.16285381930981002 30 0.20679850071086986 31 0.2119684632286416 32 0.264960579035802 33 0.3308776011373918 34 0.34897246994959286 35 0.3825772263151092 36 0.4265219077161691 37 0.4510792296755849 38 0.4782215328938865 39 0.47175907974667186 40 0.5247511955538322 41 0.5221662142949464 42 0.6320279177975959 43 0.6216879927620524 44 0.6229804833914954 45 0.6785575804575417 46 0.6837275429753135 47 0.7186247899702727 48 0.7703244151479902 49 0.7095773555641721 50 0.7561070182241179 51 0.753522036965232 52 0.8078066434018353 53 0.8233165309551507 54 0.8620912498384388 55 0.8310714747318083 56 0.8336564559906942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 77370.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.067985007108696 #Duplication Level Percentage of deduplicated Percentage of total 1 69.07420095925194 22.150704407393047 2 10.813751964854298 6.935504717590797 3 5.473378743299343 5.265606824350524 4 3.014791826206118 3.867131963293266 5 2.1966063439603403 3.522036965232002 6 1.7129499012534763 3.2958511050794885 7 1.2172020474789407 2.732325190642368 8 0.8746120672282455 2.243763732712938 9 0.794002660110435 2.2915858860023266 >10 4.340816573294103 24.39705312136487 >50 0.29019386562411836 6.455990694067467 >100 0.17734069565918342 10.634612899056481 >500 0.01612188142356213 4.539227090603593 >1k 0.004030470355890533 1.6686054026108312 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1291 1.6686054026108312 No Hit GCTCAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 970 1.2537159105596485 No Hit GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 874 1.1296368101331267 No Hit GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 856 1.1063719788031536 No Hit GCTCAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 812 1.0495023911076644 No Hit GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 474 0.6126405583559519 No Hit GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 397 0.5131187798888458 No Hit GCTCAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 391 0.5053638361121882 No Hit GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 368 0.4756365516350006 No Hit GCTCAAGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC 363 0.46917409848778596 No Hit GCTCAAGGGGTGCAGTGCTGCTCAAGGGCACAACCTATCTCAGAAGTTGTAGATGA 346 0.44720175778725607 No Hit GCTCAAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 263 0.3399250355434923 No Hit GCTCAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 233 0.3011503166602042 No Hit GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 229 0.29598035414243246 No Hit GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACTGTAGCGCAGGCCGC 221 0.28564042910688897 No Hit GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 213 0.2753005040713455 No Hit GCTCAAGGGGCCCTGGCGGCCCGGAGCCGCGCTCGTTGGGCTCCCATCCTGGGTCC 212 0.27400801344190256 No Hit GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 201 0.2597906165180302 No Hit GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 192 0.2481582008530438 No Hit GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 188 0.24298823833527206 No Hit GCTCAAGGGGGCTAGTCCTTCTTAAAGCTCTAGCCAGTGGGAAAGCGTTTGTGTTT 181 0.23394080392917152 No Hit GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 179 0.23135582267028565 No Hit GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 174 0.22489336952307096 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 174 0.22489336952307096 No Hit GCTCAAGGGATTCTCGCAAGGAAAACATTGCCACCATTTGTAGAACATGTAATCTT 172 0.2223083882641851 No Hit GCTCAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC 169 0.2184309163758563 No Hit GCTCAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 167 0.21584593511697042 No Hit GCTCAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 164 0.2119684632286416 No Hit GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.19775106630476927 No Hit GCTCAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 143 0.1848261600103399 No Hit GCTCAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT 136 0.17577872560423938 No Hit GCTCAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG 134 0.1731937443453535 No Hit GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 133 0.17190125371591056 No Hit GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATC 133 0.17190125371591056 No Hit GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 127 0.16414630993925294 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 125 0.16156132868036707 No Hit GCTCAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 125 0.16156132868036707 No Hit GCTCAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC 122 0.15768385679203825 No Hit GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.15509887553315238 No Hit GCTCAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 120 0.15509887553315238 No Hit GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 119 0.15380638490370943 No Hit GCTCAAGGGGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTG 115 0.1486364223859377 No Hit GCTCAAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA 114 0.14734393175649477 No Hit GCTCAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA 112 0.1447589504976089 No Hit GCTCAAGGGATGTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGA 111 0.14346645986816595 No Hit GCTCAAGGGAGACCAGAAATATTTTATTGTTCTTGGAATCATATTTTTGTGCTTCC 106 0.13700400672095128 No Hit GCTCAAGGGAGTGAAGTGGGGAAAATTAGCACCCTAAATCTTGTGATCTACACAGG 104 0.1344190254620654 No Hit GCTCAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT 103 0.13312653483262246 No Hit GCTCAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGA 102 0.13183404420317954 No Hit GCTCAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 100 0.12924906294429364 No Hit GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 98 0.12666408168540777 No Hit GCTCAAGGGGAAGCCAGCTTGGAGCACAGTGAGATGCTGAGGAATACCAGCCTCCA 94 0.12149411916763603 No Hit GCTCAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 93 0.12020162853819309 No Hit GCTCAAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG 93 0.12020162853819309 No Hit GCTCAAGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGGTCTGGGA 93 0.12020162853819309 No Hit GCTCAAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC 92 0.11890913790875016 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC 88 0.11373917539097841 No Hit GCTCAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAG 88 0.11373917539097841 No Hit GCTCAAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCG 87 0.11244668476153548 No Hit GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGT 86 0.11115419413209254 No Hit GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 86 0.11115419413209254 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCC 82 0.1059842316143208 No Hit GCTCAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG 82 0.1059842316143208 No Hit GCTCAAGGGGAGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 82 0.1059842316143208 No Hit GCTCAAGGGGGGGAGTAAAGAGTTCTACAAAAGGTAGCTGTGAAAATATACATACA 82 0.1059842316143208 No Hit GCTCAAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGCTC 81 0.10469174098487787 No Hit GCTCAAGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 80 0.10339925035543493 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 80 0.10339925035543493 No Hit GCTCAAGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACT 80 0.10339925035543493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002584981258885873 0.0 0.0 0.0 0.0 11 0.0038774718883288098 0.0 0.0 0.0 0.0 12 0.005169962517771746 0.0 0.0 0.0 0.0 13 0.005169962517771746 0.0 0.0 0.0 0.0 14 0.006462453147214683 0.0 0.0 0.0 0.0 15 0.006462453147214683 0.0 0.0 0.0 0.0 16 0.006462453147214683 0.0 0.0 0.0 0.0 17 0.006462453147214683 0.0 0.0 0.0 0.0 18 0.006462453147214683 0.0 0.0 0.0 0.0 19 0.006462453147214683 0.0 0.0 0.0 0.0 20 0.009047434406100556 0.0 0.0 0.0 0.0 21 0.009047434406100556 0.0 0.0 0.0 0.0 22 0.010339925035543492 0.0 0.0 0.0 0.0 23 0.010339925035543492 0.0 0.0 0.0 0.0 24 0.011632415664986429 0.0 0.0 0.0 0.0 25 0.011632415664986429 0.0 0.0 0.0 0.0 26 0.011632415664986429 0.0 0.0 0.0 0.0 27 0.011632415664986429 0.0 0.0 0.0 0.0 28 0.019387359441644048 0.0 0.0 0.0 0.0 29 0.023264831329972858 0.0 0.0 0.0 0.0 30 0.03360475636551635 0.0 0.0 0.0 0.0 31 0.03489724699495929 0.0 0.0 0.0 0.0 32 0.036189737624402224 0.0 0.0 0.0 0.0 33 0.036189737624402224 0.0 0.0 0.0 0.0 34 0.03748222825384516 0.0 0.0 0.0 0.0 35 0.054284606436603336 0.0 0.0 0.0 0.0 36 0.054284606436603336 0.0 0.0 0.0 0.0 37 0.05686958769548921 0.0 0.0 0.0 0.0 38 0.05816207832493214 0.0 0.0 0.0 0.0 39 0.05816207832493214 0.0 0.0 0.0 0.0 40 0.060747059583818014 0.0 0.0 0.0 0.0 41 0.06333204084270389 0.0 0.0 0.0 0.0 42 0.06462453147214682 0.0 0.0 0.0 0.0 43 0.0672095127310327 0.0 0.0 0.0 0.0 44 0.06979449398991858 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAAGC 25 2.0002364E-5 50.28702 50 CTGATAC 25 2.0002364E-5 50.28702 49 TGATACA 25 2.0002364E-5 50.28702 50 ACATCGA 40 2.4974725E-9 50.28702 50 ACACAGG 20 4.0434883E-4 50.28702 50 TATTATA 20 4.0434883E-4 50.28702 49 AGATTAA 25 2.0002364E-5 50.28702 49 AACGCAC 15 0.008213273 50.287018 49 AAGTACA 15 0.008213273 50.287018 49 TAAGTGT 15 0.008213273 50.287018 50 ACGCACC 15 0.008213273 50.287018 50 TTATTTG 15 0.008213273 50.287018 50 TAATCTT 15 0.008213273 50.287018 50 GCGCCAG 15 0.008213273 50.287018 49 TTAAGCT 15 0.008213273 50.287018 49 TCTCAGG 15 0.008213273 50.287018 49 GTAATCT 15 0.008213273 50.287018 49 TAGGCTA 15 0.008213273 50.287018 49 AAATTCG 25 2.0079793E-5 50.254242 48 CCGTAAA 25 2.0079793E-5 50.254242 43 >>END_MODULE