SRR212493_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212493_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74020
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	1298	1.7535801134828426	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1202	1.623885436368549	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	783	1.0578222102134558	No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	781	1.0551202377735747	No Hit
GCTCAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	738	0.9970278303161307	No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	606	0.8186976492839773	No Hit
GCTCAAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	407	0.5498513915158065	No Hit
GCTCAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	407	0.5498513915158065	No Hit
GCTCAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	369	0.49851391515806537	No Hit
GCTCAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	361	0.4877060253985409	No Hit
GCTCAAGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC	350	0.4728451769791948	No Hit
GCTCAAGGGGTGCAGTGCTGCTCAAGGGCACAACCTATCTCAGAAGTTGTAGATGA	346	0.4674412320994326	No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	336	0.453931369900027	No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	236	0.3188327479059714	No Hit
GCTCAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	235	0.3174817616860308	No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	214	0.2891110510672791	No Hit
GCTCAAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACTGTAGCGCAGGCCGC	213	0.2877600648473385	No Hit
GCTCAAGGGGCCCTGGCGGCCCGGAGCCGCGCTCGTTGGGCTCCCATCCTGGGTCC	208	0.28100513374763575	No Hit
GCTCAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	204	0.27560118886787355	No Hit
GCTCAAGGGATTCTCGCAAGGAAAACATTGCCACCATTTGTAGAACATGTAATCTT	187	0.25263442312888407	No Hit
GCTCAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG	185	0.24993245068900297	No Hit
GCTCAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	183	0.24723047824912187	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	182	0.2458794920291813	No Hit
GCTCAAGGGGGCTAGTCCTTCTTAAAGCTCTAGCCAGTGGGAAAGCGTTTGTGTTT	158	0.21345582275060795	No Hit
GCTCAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	157	0.21210483653066736	No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156	0.21075385031072685	No Hit
GCTCAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA	155	0.2094028640907863	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	145	0.1958930018913807	No Hit
GCTCAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	144	0.19454201567144014	No Hit
GCTCAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC	142	0.19184004323155904	No Hit
GCTCAAGGGGAGGCCTGCACGGCAGAGGGGGCAAATGCCTTCCAGCGCTGGAATTG	137	0.18508511213185624	No Hit
GCTCAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT	132	0.17833018103215345	No Hit
GCTCAAGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA	131	0.17697919481221291	No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC	123	0.16617130505268846	No Hit
GCTCAAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATC	121	0.16346933261280736	No Hit
GCTCAAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA	119	0.16076736017292623	No Hit
GCTCAAGGGGAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAG	109	0.14725749797352067	No Hit
GCTCAAGGGATGTATACCGACTTAGCCACAATCTATGAACGTGCCGGTCGAGTGGA	108	0.1459065117535801	No Hit
GCTCAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG	108	0.1459065117535801	No Hit
GCTCAAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCG	107	0.14455552553363954	No Hit
GCTCAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGC	107	0.14455552553363954	No Hit
GCTCAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAG	106	0.143204539313699	No Hit
GCTCAAGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC	103	0.13915158065387734	No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	100	0.13509862199405567	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	100	0.13509862199405567	No Hit
GCTCAAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG	98	0.13239664955417454	No Hit
GCTCAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	96	0.12969467711429344	No Hit
GCTCAAGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	95	0.12834369089435288	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTCTTCC	95	0.12834369089435288	No Hit
GCTCAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	94	0.12699270467441232	No Hit
GCTCAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGT	93	0.12564171845447178	No Hit
GCTCAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT	93	0.12564171845447178	No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC	91	0.12293974601459065	No Hit
GCTCAAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	91	0.12293974601459065	No Hit
GCTCAAGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGGTCTGGGA	90	0.1215887597946501	No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90	0.1215887597946501	No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA	89	0.12023777357470954	No Hit
GCTCAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	87	0.11753580113482844	No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	86	0.11618481491488786	No Hit
GCTCAAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGAT	85	0.1148338286949473	No Hit
GCTCAAGGGAGTGAAGTGGGGAAAATTAGCACCCTAAATCTTGTGATCTACACAGG	84	0.11348284247500676	No Hit
GCTCAAGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	83	0.11213185625506619	No Hit
GCTCAAGGGAGACCAGAAATATTTTATTGTTCTTGGAATCATATTTTTGTGCTTCC	83	0.11213185625506619	No Hit
GCTCAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	79	0.10672791137530398	No Hit
GCTCAAGGGGGGAATTCCTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	79	0.10672791137530398	No Hit
GCTCAAGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGC	78	0.10537692515536343	No Hit
GCTCAAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	77	0.10402593893542285	No Hit
GCTCAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG	76	0.1026749527154823	No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	76	0.1026749527154823	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGATG	40	2.5229383E-9	50.22109	49
ATCCTGG	25	2.0150012E-5	50.22109	46
AGAAGTT	40	2.5229383E-9	50.22109	42
TGGTATG	20	4.0686445E-4	50.22109	50
TTCAACA	20	4.0686445E-4	50.22109	50
AACCCCG	20	4.0686445E-4	50.22109	50
TTATACT	25	2.0150012E-5	50.22109	43
ATTATAC	25	2.0150012E-5	50.22109	42
GTCTTTA	20	4.0686445E-4	50.22109	43
AGTCGCG	40	2.5229383E-9	50.22109	43
TCTTGCT	20	4.0686445E-4	50.22109	44
TGTAGAT	40	2.5229383E-9	50.22109	48
TTGGTAT	20	4.0686445E-4	50.22109	49
GACCAGT	20	4.0686445E-4	50.22109	44
TAGATGA	40	2.5229383E-9	50.22109	50
AGTAGGA	15	0.0082546305	50.221085	48
GTCGCGT	35	5.021502E-8	50.221085	44
CGAGGGA	30	1.0034219E-6	50.221085	47
AATAGTA	15	0.0082546305	50.221085	45
CCATCGA	15	0.0082546305	50.221085	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content