FastQCFastQC Report
Tue 31 May 2016
SRR212492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99903
Sequences flagged as poor quality0
Sequence length58
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT39963.999879883486982No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14791.480436022942254No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11991.2001641592344574No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC11181.1190855129475592No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6360.6366175189934236No Hit
ATTCCAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC6000.6005825650881356No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.5965786813208812No Hit
ATTCCAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5500.5505340179974575No Hit
ATTCCAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5350.5355194538702541No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATTCCAGGGAAGCAGTGG4570.4574437204087965No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4430.4434301272234067No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.39538352201635585No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.3753641031800847No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.3493388586929322No Hit
ATTCCAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2660.26625827052240675No Hit
ATTCCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2120.21220583966447454No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1980.1981922464790847No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1810.18117574046825421Illumina Single End Adapter 2 (96% over 25bp)
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC1370.13713301902845762No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC1340.13413010620301694No Hit
ATTCCAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1310.13112719337757625No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG1280.12812428055213557No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.11310971642493217No Hit
ATTCCAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.11210874548311861No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT1090.10910583265767795No Hit
ATTCCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.10710389077405083No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT1000.10009709418135591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATGC700.052.07827852
TAGCGCA2000.052.07827443
CAATGCG150.00715820452.07827451
CTGGGAA150.00715820452.07827445
GGCCGCT1900.052.07827450
CCGCCCC550.052.07827452
CCGCTCC1900.052.07827452
TCGAACT650.052.07827452
GGATTTG150.00715820452.07827446
AGGCCGC2400.052.07827449
CGCAGGC2400.052.07827446
GCCGCCC550.052.07827451
CAGGCCG2400.052.07827448
GTTGCAA150.00715820452.07827447
TATGGAT203.407E-452.07827452
GCGGGTA150.00715820452.07827445
TTCCCGG150.007172426752.0521642
GTCTCGT150.007172426752.0521642
GAGGTGG150.007186670352.02606630
TTCGCTA2400.052.02606633
Produced by FastQC (version 0.11.5)