FastQCFastQC Report
Tue 31 May 2016
SRR212491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99441
Sequences flagged as poor quality0
Sequence length58
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT40744.096901680393399No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG16041.6130167637091342No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11831.1896501443066743No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC10311.0367956878953348No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.6446033326293984No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6180.6214740398829456No Hit
ATTCCAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC6030.6063897185265634No Hit
ATTCCAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5800.5832604257801108No Hit
ATTCCAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5040.5068331975744411No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4230.42537786224997737No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATTCCAGGGAAGCAGTGG3670.36906306251948395No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.3499562554680665No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.3439225269255136No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.3268269627216138No Hit
ATTCCAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2590.2604559487535323No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC2210.2222423346506974Illumina Single End Adapter 2 (96% over 25bp)
ATTCCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2020.20313552759927997No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1960.19710179905672712No Hit
ATTCCAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1510.1518488349875806No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC1400.14078699932623365No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT1290.12972516366488673No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC1260.12670829939361028No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG1260.12670829939361028No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG1200.12067457085105741No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.11866332800353979No Hit
ATTCCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.11866332800353979No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1150.11564646373226335No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT1110.11162397803722811No Hit
ATTCCAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.1025733852233988No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA1010.10156776379963998No Hit
ATTCCAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.10156776379963998No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1000.10056214237588118Illumina Single End Adapter 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAA150.00712658252.13636427
CAGAGGG251.6188553E-552.13636427
ATATTAG600.052.13636427
AACGCGG203.396658E-452.1100523
CGCAGGA203.396658E-452.1100525
CTCTGCA150.007140835352.11004626
AACGCGA307.805902E-752.11004623
CAACATC150.007140835352.11004624
AACCCAT600.052.11004622
CCCATAT600.052.11004624
CCTCAGT150.007140835352.11004626
ACCCATA600.052.11004623
CATATTA600.052.11004626
ACGCGAT251.623706E-552.11004624
ACAACAT150.007140835352.11004623
AACATCT150.007140835352.11004625
CAACGCG503.6379788E-1252.11004622
AAACCCA600.052.08375521
TGATCAT150.007155109752.08375551
GGCCCCT150.007155109752.08375550
Produced by FastQC (version 0.11.5)